Faculty of Biological Sciences

Prof Michelle Peckham

BA, York; PhD 1984, London.
Professor of Cell Biology
School of Molecular and Cellular Biology

Background: Our research group works broadly on the cytoskeleton and cytoskeletal molecular motors, myosins and kinesins, to understand the structure, function and how the activity of these proteins are regulated in cells, as well as how these proteins are implicated in and contribute to disease processes. The involvement of many muscle myosins in heart and skeletal muscle disease has led to us developing an interest in muscle development, and the contribution of satellite cells (muscle stem cells) to muscle formation. We use a wide range of tools and approaches to address key questions about molecular motors, that include a wide range of cell and molecular biology techniques, protein expression and purification, as well as light microscopy, electron microscopy, X-ray crystallography, NMR, AFM and other biophysical approaches, ofter through collaborating with other research groups at Leeds. We are also developing 'super-resolution' imaging approaches, including PALM/STORM, and iSIM.

Contact:  Astbury 8.106, +44(0) 113 34 34348, email address for  

You can read more about Prof Peckham's interests here:
www.contractility.org

Research Interests

Myosins, motors, and muscle in health and disease

Our laboratory is interested in the cytoskeleton, from basic research into how myosins perform their functions in cells, to how mutations in cytoskeletal proteins cause disease.

For example, we recently discovered that many different types of myosin are overexpressed in prostate cancer and this contributes to the cellular phenotype and metastatic potential (Makowska et al., Cell Reports, 2015). The image below shows how the cytoskeleton changes when different myosin isoforms are depleted (from left to right: wild type cells, Myo1b, Myo9b, Myo10 and Myo18a knockdown cells: cells are stained for actin (red) and non-muscle myosin 2A (green)).

 prostate cancer cells

We collaborate with a company (Orla Protein Technologies) to develop new methodologies to better differentiate muscle cells in culture, which helps us investigate the effects of mutations in muscle sarcomeric proteins on disease (see our recent paper in Cytotechnology, 2016, Parker et al.)  The image below shows a human skeletal myotube differentiated on an Orla Surface, stained for skeletal myosin (green).

myotube in culture

 We have a very strong interest in imaging, including super-resolution imaging. We have built a 3D PALM/STORM system, that allows a resolution of ~10nm (about 20 times better than a normal wide-field microscope), and an iSIM (instant structured illumination microscope) which is very good for fast live cell imaging. The image below shows a 3D STORM image of the actin cytoskeleton in a cell.

super-resolution image

 

We are also interested in the structure and regulation of cytoskeletal proteins, recently obtaining crystal structures of the calponin homology domains of alpha-actinin, both wild type and mutated isoforms (see Haywood et al., 2016, Biochem. J.) And we are starting to use other structural approaches (Cryo-EM and NMR) to investigate protein structure.

 

Current Projects

We are funded by BBSRC to investigate the structure and function of stable single alpha helical domains. These domains are found in myosins and a wide range of other proteins, and appear to act as 'constant force springs' (Wolny et al., J. Biol. Chem. 2014). We think that they can unfold at low forces and then refold, which means that a force applied to the protein will unfold the SAH domain, but allow domains in the protein either side of the SAH domain to remain attached to their binding partners. 

We are also working with Stefan Kepinski on gravitropism in plants (BBSRC funded) and with Colin Johnson on primary cilia (BBSRC funded).

We are funded by the British Heart Foundation to investigate how mutations in slow (beta-cardiac) myosin heavy chain in the coiled-coil cause heart disease. We are using a combination of protein structure determination and cell biology to investigate how mutations affect the structure of the coiled-coil to understand this process.

We are funded by MRC to build and develop super-resolution imaging technologies such as PALM/STORM and iSIM. These technologies break or overcome the resolution limit of a normal wide-field microscope, allowing us to see a more detailed view of cellular structures. We have also recently built a simple light sheet microscope (Open SPIM).

We are funded by the Wellcome Trust to investigate how the activity of non-muscle myosin isoforms are regulated in cells. Non-muscle myosins are self-regulating. For example, the tail of the myosin interacts with the motor domain to prevent the motor from interacting with its actin track in myosin 5, 7 and 2, and probably many other myosins. (e.g. Baboolal et al., PNAS 2009). What is the nature of this interaction? How is this overcome so that the motor can be switched on? 

PhD students in the lab are also studying aspects of these problems, including modelling of myosin 7 (EPSRC funded, with Sarah Harris, Oliver Harlen and Daniel Read in MAPS), using super-resolution microscopy, crystallography and electron microscopy to study the Z-disc (BBSRC DTP funded, with Neil Ranson and Thomas Edwards in FBS), super-resolution imaging of primary cilia (with Colin Johnson in teh faculty of medicine and health),  investigating the effects of mutations in the Z-disc protein, alpha-actinin, investigating ASPM, a protein involved in mitosis, the study of myosin 5 (with Peter Knight and Jim Sellers (NIH),  super-resolution imaging of virus complexes in cells (with Mark Harris, Wellcome Trust funded).

 

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Imaging, Microscopy, cytoskeleton, diseases linked to cytoskeletal proteins (including proteins in the muscle cytoskeleton)

Postgraduate studentship areas:

  • We have several PhD studentship opportunities available
  • (see https://www.findaphd.com/search/phd.aspx?keywords=Peckham)
  • These include:
  • 1. A directly funded BBSRC iCASE studentship with Orla Protein Technologies (closing date Monday January 16th 2017):
  • Developing and optimising defined tissue culture surfaces for cellular differentiation of muscle.
  • This project will investigate muscle differentiation on novel surfaces, and develop new and better ways for differentiating muscle in culture. Working with Orla, the student will generate new peptides to support and promote differentiation, and we will generate patterned surfaces, and being to work on differentiation in 3D. The project will use imaging to assess differentiation, including some 'super-resolution' microscopy and light sheet microscopy.
  • 2. Folding and regulation of non-muscle myosin 2A in vitro and in vivo, a mechanistic understanding. BBSRC DTP studentship (open competition). closing date Thursday January 5th, 2017.
  • Non-muscle myosin 2A (NM2A) is implicated in a range of diseases from cancer, to defective blood clotting and deafness. Common to all of these diseases is the likelihood that the regulation of NM2A is disrupted, however we only have a poor understanding of the structure and regulation of NM2A.The aim of this project is to use a combination of cell and structural biology, including high resolution cryo-EM and super-resolution light microscopy, to gain a detailed mechanistic insight into how NM2A activity is regulated in cells, and how this regulation is disrupted in disease.
  • 3. A mechanistic understanding of the role of non-muscle myosin 2A in platelet disorders. BHF funded studentship (Open competition) closing date 3 January 2017. See http://www.cardiovascular.leeds.ac.uk/opportunities/ for more details.
  • 4. DiMeN Doctoral Training Partnership (MRC): Understanding how mutations in myosin 7a contribute to sight and hearing loss (with Walter Marcotti, University of Sheffield). Closing date Friday January 6th, 2017. (Open Competition). This project will use a combination of cell and molecular biology, structural approaches, and super-resolution microscopy. See details on the 'findaphD' website.

See also:

Modules managed

BIOL2211 - Human Diseases

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC3111/12/BIOL3112 B - ATU - Cytoskeletal Molecular Motors
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOL1302 - Introductory Skills for Biological Sciences
BIOL2211 - Human Diseases
BIOL3306 - Biological Sciences Research Project
BIOL5112M/5312M - Bioimaging
BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5392M - Bioscience MSc Research Project
BMSC3101 - Inherited Disorders
DSUR1127 - Health and Health promotion
FOBS1201/BIOL1214 - Molecular Physiology/Multicellular Systems
MICR2120/BIOC2301 - Integrated Biochemistry/Medical Bacteriology
MICR3110 - Medical Microbiology Research Project

Centre memberships:

Group Leader Prof Michelle Peckham  (Professor of Cell Biology)

Myosins, motors, and muscle in health and disease 

Dr Matthew Batchelor  (Research Fellow)

Dr Sally Boxall  (Research Facility Manager)

Dr Alistair Curd  (Research Fellow)

Dr Marta Kurzawa  (Research Technician)

Dr Francine Parker  (Research Fellow)

Dr Marcin Wolny  (Research Fellow)


Postgraduates

Glenn Carrington (Primary supervisor) 50% FTE
Sophie Hesketh (Primary supervisor) 50% FTE
Marcus Holt (Primary supervisor) 60% FTE
Brendan Rogers (Primary supervisor) 34% FTE
Christopher Bartlett (Co-supervisor) 50% FTE
Anna Lopata (Co-supervisor) 50% FTE
Rebecca Perrin (Co-supervisor) 40% FTE

Baboolal TG, Sakamoto T, Forgacs E, White HD, Jackson SM, Takagi Y, Farrow RE, Molloy JE, Knight PJ, Sellers JR, Peckham M The SAH domain extends the functional length of the myosin lever P NATL ACAD SCI USA 106 22193-22198, 2009
DOI:10.1073/pnas.0909851106

Yang Y, Baboolal TG, Siththanandan V, Chen M, Walker ML, Knight PJ, Peckham M, Sellers JR A FERM domain autoregulates Drosophila myosin 7a activity P NATL ACAD SCI USA 106 4189-4194, 2009
DOI:10.1073/pnas.0808682106

Dunn S, Morrison EE, Liverpool TB, Molina-Paris C, Cross RA, Alonso MC, Peckham M Differential trafficking of Kif5c on tyrosinated and detyrosinated microtubules in live cells J CELL SCI 121 1085-1095, 2008
DOI:10.1242/jcs.026492

Parker F, White K, Phillips S, Peckham M Promoting differentiation of cultured myoblasts using biomimetic surfaces that present alpha-laminin-2 peptides Cytotechnology 68 2159-2169, 2016
DOI:10.1007/s10616-016-0006-y
View abstract

Haywood NJ, Wolny M, Rogers B, Trinh CH, Shuping Y, Edwards TA, Peckham M Hypertrophic cardiomyopathy mutations in the calponin-homology domain of ACTN2 affect actin binding and cardiomyocyte Z-disc incorporation Biochemical Journal 473 2485-2493, 2016
DOI:10.1042/BCJ20160421
View abstract

Peckham M How myosin organization of the actin cytoskeleton contributes to the cancer phenotype. Biochemical Society transactions 44 1026-1034, 2016
DOI:10.1042/bst20160034
View abstract

Howe A, Streetley J, Peckham M, Rosenthal PB, Molloy JE Structure and Dynamics of Filopodia Studied by Electron Cryo-Tomography and Single Molecule Fluorescence Imaging, 2016

Lambacher NJ, Bruel AL, Van Dam TJP, Szymaska K, Slaats GG, Kuhns S, McManus GJ, Kennedy JE, Gaff K, Wu KM, Van Der Lee R, Burglen L, Doummar D, Rivière JB, Faivre L, Attié-Bitach T, Saunier S, Curd A, Peckham M, Giles RH, Johnson CA, Huynen MA, Thauvin-Robinet C, Blacque OE TMEM107 recruits ciliopathy proteins to subdomains of the ciliary transition zone and causes Joubert syndrome Nature Cell Biology 18 122-131, 2016
DOI:10.1038/ncb3273
View abstract

Bacon T, Seiler C, Wolny M, Hughes R, Watson P, Schwabe J, Grigg R, Peckham M Histone deacetylase 3 indirectly modulates tubulin acetylation Biochemical Journal 472 367-377, 2015
DOI:10.1042/BJ20150660
View abstract

Makowska KA, Hughes RE, White KJ, Wells CM, Peckham M Specific Myosins Control Actin Organization, Cell Morphology, and Migration in Prostate Cancer Cells Cell Reports 13 2118-2125, 2015
DOI:10.1016/j.celrep.2015.11.012
View abstract

Knight AE, Peckham M Recent innovations in super-resolution microscopy METHODS 88 1-2, 2015
DOI:10.1016/j.ymeth.2015.08.004

Samejima K, Platani M, Wolny M, Ogawa H, Vargiu G, Knight PJ, Peckham M, Earnshaw WC The Inner Centromere Protein (INCENP) Coil Is a Singleα-Helix (SAH) Domain That Binds Directly to Microtubules and Is Important for Chromosome Passenger Complex (CPC) Localization and Function in Mitosis Journal of Biological Chemistry 290 21460-21472, 2015
DOI:10.1074/jbc.M115.645317
View abstract

Batchelor M, Wolny M, Dougan L, Paci E, Knight PJ, Peckham M Myosin tails and singleα-helical domains Biochemical Society Transactions 43 58-63, 2015
DOI:10.1042/BST20140302
View abstract

Wolny MD, Batchelor MR, Knight PJ, Paci E, Peckham M Determining How Many Ionic Interactions are Needed for the High Stability of Single Alpha Helical (SAH) Domains, 2015

Curd A, Cleasby A, Makowska K, York A, Shroff H, Peckham M Construction of an instant structured illumination microscope Methods 88 37-47, 2015
DOI:10.1016/j.ymeth.2015.07.012
View abstract

Wolny M, Batchelor M, Knight PJ, Paci E, Dougan L, Peckham M Stable singleα-helices are constant force springs in proteins. Journal of Biological Chemistry 289 27825-27835, 2014
DOI:10.1074/jbc.M114.585679
View abstract

Soppina V, Norris SR, Dizaji AS, Kortus M, Veatch S, Peckham M, Verhey KJ Dimerization of mammalian kinesin-3 motors results in superprocessive motion. Proc Natl Acad Sci U S A 111 5562-5567, 2014
DOI:10.1073/pnas.1400759111
View abstract

Colegrave M, Peckham M Structural implications ofβ-cardiac myosin heavy chain mutations in human disease Anatomical Record 297 1670-1680, 2014
DOI:10.1002/ar.22973
View abstract

Wolny M, Colegrave M, Colman L, White E, Knight PJ, Peckham M Erratum: Cardiomyopathy mutations in the tail ofβ-Cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes (Journal of Biological Chemistry (2013) 288 (36260)) Journal of Biological Chemistry 288 36260-, 2013
DOI:10.1074/jbc.A113.513291

Wolny M, Colegrave M, Colman L, White E, Knight P, Peckham M Cardiomyopathy mutations in the tail of beta cardiac myosin modify the coiled-coil structure and affect integration into thick filaments in muscle sarcomeres in adult cardiomyocytes. Journal of Biological Chemistry 288 31952-31962, 2013
DOI:10.1074/jbc.M113.513291
View abstract

Riches K, Franklin L, Maqbool A, Peckham M, Adams M, Bond J, Warburton P, Feric NT, Koschinsky ML, O'Regan DJ, Ball SG, Turner NA, Porter KE Apolipoprotein(a) acts as a chemorepellent to human vascular smooth muscle cells via integrin alpha(v)beta(3) and RhoA/ROCK-mediated mechanisms INTERNATIONAL JOURNAL OF BIOCHEMISTRY&CELL BIOLOGY 45 1776-1783, 2013
DOI:10.1016/j.biocel.2013.05.021

Sevdali M, Kumar V, Peckham M, Sparrow J Human congenital myopathy actin mutants cause myopathy and alter Z-disc structure in Drosophila flight muscle. Neuromuscular disorders : NMD 23 243-255, 2013
View abstract

Stones R, Benoist D, Peckham M, White E Microtubule proliferation in right ventricular myocytes of rats with monocrotaline-induced pulmonary hypertension. J Mol Cell Cardiol 56 91-96, 2013
DOI:10.1016/j.yjmcc.2012.12.010
View abstract

Peckham M, Takagi Y, Ushakov D, Ma X, Temprine K, Shroff H 3D PALM shows distinct distributions of Z-disc proteins with the Z-discs in cardiomyocytes. MOLECULAR BIOLOGY OF THE CELL 23 -, 2012

Yoon JR, Whipple RA, Balzer EM, Cho EH, Matrone MA, Peckham M, Martin SS Local anesthetics inhibit kinesin motility and microtentacle protrusions in human epithelial and breast tumor cells BREAST CANCER RES TR 129 691-701, 2011
DOI:10.1007/s10549-010-1239-7

Peckham M Coiled coils and SAH domains in cytoskeletal molecular motors BIOCHEM SOC T 39 1142-1148, 2011
DOI:10.1042/BST0391142

Nenasheva TA, Mashanov GI, Peckham M, Molloy JE Imaging Individual Myosin Molecules Within Living Cells In Single Molecule Enzymology , 2011
DOI:10.1007/978-1-61779-261-8_9
View abstract

Allsop G, Peckham M Cytoskeleton and Cell Motility In Comprehensive Biotechnology , 2011

Peckham M Histology at a Glance, 2011
View abstract

Thompson O, Moore CJ, Hussaina SA, Kleino I, Peckham M, Hohenester E, Ayscough KR, Saksela K, Winder SJ Modulation of cell spreading and cell-substrate adhesion dynamics by dystroglycan J CELL SCI 123 118-127, 2010
DOI:10.1242/jcs.047902

Peckham M Journal club. A cell biologist ponders an outstanding mystery in muscle formation. Nature 458 1081-, 2009

Sellers JR, Yang Y, Baboolal T, Siththanandan V, Walker ML, Knight PJ, Peckham M Structural Basis for The Regulation of Drosophila Myosin 7a Biophysical Journal 96 3a-4a, 2009
DOI:10.1016/j.bpj.2008.12.910

Takagi Y, Farrow RE, Mashanov GI, Batters C, Yang Y, Peckham M, Sellers JR, Molloy JE Single Molecule Investigation of the Acto-Myosin-10 Complex Using Optical Tweezers Biophysical Journal 96 139a-140a, 2009
DOI:10.1016/j.bpj.2008.12.3871

Peckham M, Knight PJ When a predicted coiled coil is really a single alpha-helix, in myosins and other proteins SOFT MATTER 5 2493-2503, 2009
DOI:10.1039/b822339d

Ridley A, Peckham M, Clark P Introduction Journal of Microscopy 231 440-, 2008
DOI:10.1111/j.1365-2818.2008.02067.x

Peltzer J, Colman L, Cebrian J, Musa H, Peckham M, Keller A Novel murine clonal cell lines either express slow or mixed (fast and slow) muscle markers following differentiation in vitro DEV DYNAM 237 1412-1423, 2008
DOI:10.1002/dvdy.21543

Domazetovska A, Ilkovski B, Kumar V, Valova VA, Vandebrouck A, Hutchinson DO, Robinson PJ, Cooper ST, Sparrow JC, Peckham M, North KN Intranuclear rod myopathy: Molecular pathogenesis and mechanisms of weakness ANN NEUROL 62 597-608, 2007
DOI:10.1002/ana.21200

Mashanov GI, Nenasheva TA, Peckham M, Molloy JE Cell biochemistry studied by single-molecule imaging BIOCHEM SOC T 34 983-988, 2006

Musa H, Meek S, Gautel M, Peddie D, Smith AJH, Peckham M Targeted homozygous deletion of M-band titin in cardiomyocytes prevents sarcomere formation J CELL SCI 119 4322-4331, 2006
DOI:10.1242/jcs.03198

Swailes NT, Colegrave M, Knight PJ, Peckham M Non-muscle myosins 2A and 2B drive changes in cell morphology that occur as myoblasts align and fuse J CELL SCI 119 3561-3570, 2006
DOI:10.1242/jcs.03096

Peckham M Research Highlights: Journal Club Nature pp.895-, 2006

Murphy JE, Tacon D, Tedbury PR, Hadden JM, Knowling S, Sawamura T, Peckham M, Phillips SEV, Walker JH, Ponnambalam S LOX-1 scavenger receptor mediates calcium-dependent recognition of phosphatidylserine and apoptotic cells BIOCHEM J 393 107-115, 2006

Knight PJ, Thirumurugan K, Xu YH, Wang F, Kalverda AP, Stafford WF, Sellers JR, Peckham M The predicted coiled-coil domain of myosin 10 forms a novel elongated domain that lengthens the head J BIOL CHEM 280 34702-34708, 2005
DOI:10.1074/jbc.M504887200

Swailes NT, Knight PJ, Peckham M Actin filament organization in aligned prefusion myoblasts J ANAT 205 381-391, 2004

Tacon D, Knight PJ, Peckham M Imaging myosin 10 in cells BIOCHEMICAL SOCIETY TRANSACTIONS 32 689-693, 2004

Mashanov GI, Tacon D, Peckham M, Molloy JE The spatial and temporal dynamics of pleckstrin homology domain binding at the plasma membrane measured by Imaging single molecules in live mouse myoblasts J BIOL CHEM 279 15274-15280, 2004
DOI:10.1074/jbc.M312140200

Ridley A, Clark P, Peckham M Cell Motility, from molecules to organisms, 2004

Miller G, Musa H, Gautel M, Peckham M A targeted deletion of the C-terminal end of titin, including the titin kinase domain, impairs myofibrillogenesis J CELL SCI 116 4811-4819, 2003
DOI:10.1242/jcs.00768

Miller G, Maycock J, White E, Peckham M, Calaghan S Heterologous expression of wild-type and mutant beta-cardiac myosin changes the contractile kinetics of cultured mouse myotubes. J Physiol 548 167-174, 2003
DOI:10.1113/jphysiol.2002.031922
View abstract

Musa H, Orton C, Morrison EE, Peckham M Microtubule assembly in cultured myoblasts and myotubes following nocodazole induced microtubule depolymerisation. J Muscle Res Cell Motil 24 301-308, 2003
View abstract

Mashanov GI, Tacon DS, Knight AE, Peckham M, Molloy JE Visualizing single molecules inside living cells using total internal reflection fluorescence microscopy. Methods: A Companion to Methods in Enzymology 29 142-152, 2003
DOI:10.1016/S1046-2023(02)00305-5

Clark P, Dunn GA, Knibbs A, Peckham M Alignment of myoblasts on ultrafine gratings inhibits fusion in vitro INT J BIOCHEM CELL B 34 816-825, 2002

Natali AJ, Wilson LA, Peckham M, Turner DL, Harrison SM, White E Different regional effects of voluntary exercise on the mechanical and electrical properties of rat ventricular myocytes Journal of Physiology 541 863-875, 2002
DOI:10.1113/jphysiol.2001.013415

Dobrzynski H, Musa H, Claydon TW, Coppen SR, Dupont E, Severs NJ, Peckham M, Billeter-Clark R, Boyett MR Different distribution and cellular localisation of connexin43 and a-myosin heavy chain mRNA in the sinoatrial node. Pflugers Archiv: European Journal of Physiology 443 pp.208-, 2002

Peckham M, Miller G, Wells C, Zicha D, Dunn GA Specific changes to the mechanism of cell locomotion induced by overexpression of beta-actin J CELL SCI 114 1367-1377, 2001

Miller G, Colegrave M, Peckham M N232S, G741R and D778G beta-cardiac myosin mutants, implicated in familial hypertrophic cardiomyopathy, do not disrupt myofibrillar organisation in cultured myotubes FEBS LETT 486 325-327, 2000

Maggs AM, Taylor-Harris P, Peckham M, Hughes SM Evidence for differential post-translational modifications of slow myosin heavy chain during murine skeletal muscle development. J Muscle Res Cell Motil 21 101-113, 2000
View abstract

Harris JM, Morgan JE, Rosenblatt JD, Rosenblatt D, Peckham M, Partridge T Forced MyHCIIB expression following targeted genetic manipulation of conditionally immortalized muscle precursor cells Experimental Cell Research 253 523-532, 1999

Peckham M, Wells C, Taylor-Harris P, Coles D, Zicha D, Dunn GA Using molecular genetics as a tool in understanding crawling cell locomotion in myoblasts Biochemical Society Symposium 65 281-299, 1999

Wells C, Coles D, Entwistle A, Peckham M Myogenic cells express multiple myosin isoforms J MUSCLE RES CELL M 18 501-515, 1997

Clark P, Coles D, Peckham M Preferential adhesion to and survival on patterned laminin organizes myogenesis in vitro EXP CELL RES 230 275-283, 1997

Peckham M, Young P, Gautel M Constitutive and variable regions of Z-disk titin/connectin in myofibril formation: A dominant-negative screen Cell Structure and Function 22 95-101, 1997

Clark P, Coles D, Peckham M The behaviour of myoblasts, and myotube formation, on micropatterned substrata, 1997

Peckham M Chapter 7 The cellular and molecular basis of skeletal and cardiac muscle contraction Principles of Medical Biology 4 201-237, 1996
DOI:10.1016/S1569-2582(96)80132-2
View abstract

PECKHAM M, FERENCZI MA, IRVING M A BIREFRINGENCE STUDY OF CHANGES IN MYOSIN ORIENTATION DURING RELAXATION OF SKINNED MUSCLE-FIBERS INDUCED BY PHOTOLYTIC ATP RELEASE BIOPHYS J 67 1141-1148, 1994

MORGAN JE, BEAUCHAMP JR, PAGEL CN, PECKHAM M, ATALIOTIS P, JAT PS, NOBLE MD, FARMER K, PARTRIDGE TA MYOGENIC CELL-LINES DERIVED FROM TRANSGENIC MICE CARRYING A THERMOLABILE T-ANTIGEN - A MODEL SYSTEM FOR THE DERIVATION OF TISSUE-SPECIFIC AND MUTATION-SPECIFIC CELL-LINES DEV BIOL 162 486-498, 1994

Noble M, Groves AK, Ataliotis P, Morgan J, Peckham M, Partridge T, Jat PS Biological and Molecular Approaches to the Generation of Conditionally Immortal Neural Cells Neuroprotocols 3 189-199, 1993
DOI:10.1006/ncmn.1993.1054
View abstract

PECKHAM M, CRIPPS R, WHITE D, BULLARD B MECHANICS AND PROTEIN-CONTENT OF INSECT FLIGHT MUSCLES J EXP BIOL 168 57-76, 1992

PECKHAM M, WHITE DCS MECHANICAL-PROPERTIES OF DEMEMBRANATED FLIGHT-MUSCLE FIBERS FROM A DRAGONFLY J EXP BIOL 159 135-147, 1991

Sparrow J, Drummond D, Peckham M, Hennessey E, White D Protein engineering and the study of muscle contraction in Drosophila flight muscles. J Cell Sci Suppl 14 73-78, 1991
View abstract

DRUMMOND DR, PECKHAM M, SPARROW JC, WHITE DCS ALTERATION IN CROSSBRIDGE KINETICS CAUSED BY MUTATIONS IN ACTIN NATURE 348 440-442, 1990

Peckham M, Molloy JE, Sparrow JC, White DC Physiological properties of the dorsal longitudinal flight muscle and the tergal depressor of the trochanter muscle of Drosophila melanogaster. J Muscle Res Cell Motil 11 203-215, 1990
View abstract

PECKHAM M, IRVING M MYOSIN CROSSBRIDGE ORIENTATION IN DEMEMBRANATED MUSCLE-FIBERS STUDIED BY BIREFRINGENCE AND X-RAY-DIFFRACTION MEASUREMENTS J MOL BIOL 210 113-126, 1989

Irving M, Peckham M, Ferenczi MA Birefringence as a probe of crossbridge orientation in demembranated muscle fibres. Advances in Experimental Medicine and Biology 226 299-306, 1988
View abstract

PECKHAM M, WOLEDGE RC LABILE HEAT AND CHANGES IN RATE OF RELAXATION OF FROG MUSCLES J PHYSIOL-LONDON 374 123-135, 1986

ELZINGA G, PECKHAM M, WOLEDGE RC THE SARCOMERE-LENGTH DEPENDENCE OF THE RATE OF HEAT-PRODUCTION DURING ISOMETRIC TETANIC CONTRACTION OF FROG MUSCLES J PHYSIOL-LONDON 357 495-504, 1984

Baboolal TG, Mashanov GI, Nenasheva TA, Peckham M, Molloy JE A Combination of Diffusion and Active Translocation Localizes Myosin 10 to the Filopodial Tip. The Journal of Biological Chemistry -,
DOI:10.1074/jbc.M116.730689
View abstract