Faculty of Biological Sciences

Prof Adrian Whitehouse

BSc, Sheffield; DPhil, Oxford
Professor of Molecular Virology
School of Molecular and Cellular Biology

Background: Postdoctoral Research Fellow 1994-1998, Molecular Medicine Unit, Leeds; MRC Non-Clinical Research Fellow, 1998-2001, Molecular Medicine Unit, Leeds. Appointed Lecturer 2002, Reader 2005 and to Professor in 2010.

Contact:  Garstang 9.59 | +44(0) 113 34 37096 | email address for  

You can read more about Prof Whitehouse's interests here:
www.fbs.leeds.ac.uk/staff/Whitehouse_A/

Viruses are associated with up to 20% of cancers worldwide, representing approximately 1.2 million cancer cases worldwide annually. We are primarily interested in studying the molecular biology of 2 of the 7 viruses that have been identified as human tumour viruses. 

(i) Kaposi's sarcoma associated herpesvirus (KSHV)
This is a human gamma-2 herpesviruses which has been associated with a variety of lymphoproliferative disorders including Kaposi's sarcoma (KS), primary effusion lymphoma and multicentric Castleman's disease. Widespread HIV infection has now turned KS into an epidemic disease in Africa. KS is now the most common adult tumour in parts of Africa. Like other herpesviruses, KSHV has two distinct forms of infection, latency and lytic replication. Although latency has been implicated in tumourigenesis, reactivation and lytic replication play an important part in the pathogenesis and spread of KSHV infection. Therefore, we have a major research focus to study the molecular mechanisms which regulate reactivation and lytic gene expression to provide a better understanding of KSHV pathogenesis.

(ii) Merkel cell polyomavirus (MCPyV)
Merkel cell carcinoma (MCC) is a highly aggressive human cancer of the skin that occurs in elderly and immunosuppressed patients. Merkel cell polyomavirus was discovered in 2008 and is present in 80% of human Merkel cell carcinomas. Therefore, MCPyV is likely to have a causative role in MCC. Due to its recent discovery, little is known about the link between MCPyV and MCC. Therefore, we are currently investigating the role of MCPyV encoded proteins in transformation and immortalisation of human cells.

Current Projects 

 1. Identification of essential virus-host cell interactions which are required for virus replication or transformation.

We are utilising a range of cutting-edge transcriptomic and quantitative proteomic approaches to globally identify how viral proteins affect the cellular environment. These interactions can then be verified using biochemical and con-focal imaging techniques. This is helping to identify essential virus-host cell interactions which we can target by novel antiviral strategies to inhibit virus replication and transformation.
We are particularly interested in virus-host cell interactions which
1. Regulate reactivation in Kaposi's sarcoma associated herpesvirus
2. Regulate virus RNA processing
3. Immune evasion strategies employed by oncogenic viruses
4. Virus-host cell interactions required for the aggressive metastatic potential of some virus-induced cancers

2. Structural-based rational drug design approaches to inhibit oncogenic viruses.

To date, there are limited antiviral strategies for oncogenic viruses. Although vaccines have been developed for a few of these viruses, these are not available for all the 7 oncogenic viruses, incluing KSHV and MCPyV. Therefore novel antiviral straetgies are required to combat these important human pathogens.

Upon identification of essential virus-host cell interactions using transcriptomic and quantitative proteomic approaches, we utilise a structural-based rational drug design approach to molecular model and design small molecules to inhibit these interactions. Virtual high-throughput screening campaigns are conducted from a large libraries of commercially available compounds. Docking routines and ligand-similarity searches are utilised to design compounds which have the potential to inhibit these essential virus-host cel interactions. Once the virtual high-throughput screening campaign has been performed selected compounds are then assessed in virus-based assays for antiviral activity.

 

Faculty Research and Innovation


Studentship information

Undergraduate project topics:

  • Investigation of viral-host cell interactions in oncogenic viruses
  • Development of herpesvirus-based gene delivery vectors
  • Keywords: Cancer, oncogenic viruses, herpesvirus, polyomavirus, gene therapy, viral vectors, molecular biology

Postgraduate studentship areas:

  • Virus-host cell interactions involved in viral mRNA export
  • Identification of oncoproteins encoded by McPyV
  • Development of Herpesvirus-based Gene Delivery Vectors

See also:

Admin roles

FBS Faculty Board

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC2301/MICR2120 - Integrated Biochemistry/Medical Bacteriology
BIOC3231/32/33/3900/BIOL3211/MICR3212 - ATU 26 - Molecular Oncology
BIOC3231/32/33/BIOL3211/BMSC3233/34/35/36/MICR3212 a - ATU 14 - Gene Therapy
BIOC3231/32/33/BIOL3211/BMSC3233/34/35/36/MICR3212 b - ATU 35 - Microbial and cellular systems biology
BIOC3231/32/33/BIOL3211/MICR3212 a - ATU 21 - Life of an RNA
BIOC3900/BIOL3211/5243M/BMSC3233/34/35/36/37 - ATU 07 - Cancer Therapy/Biology
BIOL2301/05/MICR2320 - Skills for Biol Sci, Biosciences and Microbiology
BIOL3306 - Biol Sciences Research Project
BIOL5262M/CMNS5710M - Molecular Diagnostics/Biosensors and drug delivery
BIOW5901X - Foundation module
BMSC2210 - Chemotherapy
MICR1120/3342 - Introduction to Microbiology
MICR1320 - Skills for Microbiologists
MICR2121 - Molecular Virology
MICR2320 - Skills for Microbiologists 2
MICR3110 - Microbiology Research Programme
MICR3211/12 - Advanced Topics Microbiology
MICR3211/BIOL3210 a - ATU 11 - Emerging infections
MICR3325 - Skills for Microbiologists 3
MICR3370 - Microbiology Research Programme

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Prof Adrian Whitehouse  (Professor of Molecular Virology)


Dr Belinda Baquero  (Research Fellow)

Dr Anja Berndt  (Research Fellow)

Dr David Hughes  (Research Fellow)

Dr Brian Jackson  (Wellcome Trust Research Fellow)

A link between mRNA export factors, genome instability and human cancer 

Miss Jennifer Wood  (Research Technician)


Postgraduates

Noor Suhana Adzahar  
Alexander Coleman  
Laura Knight  
Zoe Lear  
Nnenna Nwogu  
Christopher Owen  
Shola Richards  
Sophie Schumann  
Gabriele Stakaityte  

Jackson BR; Noerenberg M; Whitehouse A A novel mechanism inducing genome instability in Kaposi's sarcoma-associated herpesvirus infected cells. PLoS Pathog 10 e1004098-, 2014
DOI:10.1371/journal.ppat.1004098
View abstract

Stakaitytė G; Wood JJ; Knight LM; Abdul-Sada H; Adzahar NS; Nwogu N; Macdonald A; Whitehouse A Merkel cell polyomavirus: molecular insights into the most recently discovered human tumour virus. Cancers (Basel) 6 1267-1297, 2014
DOI:10.3390/cancers6031267
View abstract

Brown HF; Unger C; Whitehouse A Potential of herpesvirus saimiri-based vectors to reprogram a somatic Ewing's Sarcoma family tumor cell line Journal of Virology 87 7127-7139, 2013
DOI:10.1128/JVI.03147-12
View abstract

Schumann S; Jackson BR; Baquero-Perez B; Whitehouse A Kaposi's sarcoma-associated herpesvirus ORF57 protein: exploiting all stages of viral mRNA processing. Viruses 5 1901-1923, 2013
DOI:10.3390/v5081901
View abstract

Emmott E; Munday D; Bickerton E; Britton P; Rodgers MA; Whitehouse A; Zhou E-M; Hiscox JA The cellular interactome of the coronavirus infectious bronchitis virus nucleocapsid protein and functional implications for virus biology Journal of Virology 87 9486-9500, 2013
DOI:10.1128/JVI.00321-13
View abstract

Griffiths DA; Abdul-Sada H; Knight LM; Jackson BR; Richards K; Prescott EL; Peach AHS; Blair GE; Macdonald A; Whitehouse A Merkel Cell Polyomavirus Small T Antigen Targets the NEMO Adaptor Protein To Disrupt Inflammatory Signaling JOURNAL OF VIROLOGY 87 13853-13867, 2013
DOI:10.1128/JVI.02159-13

Ingram N; Macnab SA; Marston G; Scott N; Carr IM; Markham AF; Whitehouse A; Coletta PL The use of high-frequency ultrasound imaging and biofluorescence for in vivo evaluation of gene therapy vectors BMC Medical Imaging 13 1-9, 2013
DOI:10.1186/1471-2342-13-35
View abstract

Turrell SJ; Filby MH; Whitehouse A; Wilson AJ Cellular uptake of highly-functionalized ruthenium(II) tris-bipyridine protein-surface mimetics. Bioorg Med Chem Lett 22 985-988, 2012
DOI:10.1016/j.bmcl.2011.12.007
View abstract

Munday DC; Surtees R; Emmott E; Dove BK; Digard P; Barr JN; Whitehouse A; Matthews D; Hiscox JA Using SILAC and quantitative proteomics to investigate the interactions between viral and host proteomes. Proteomics 12 666-672, 2012
DOI:10.1002/pmic.201100488
View abstract

Jackson BR; Noerenberg M; Whitehouse A The Kaposi's Sarcoma-Associated Herpesvirus ORF57 Protein and Its Multiple Roles in mRNA Biogenesis. Front Microbiol 3 59-, 2012
DOI:10.3389/fmicb.2012.00059
View abstract

Turrell SJ; Macnab SA; Rose A; Melcher AA; Whitehouse A A Herpesvirus saimiri-based vector expressing TRAIL induces cell death in human carcinoma cell lines and multicellular spheroid cultures. Int J Oncol 40 2081-2089, 2012
DOI:10.3892/ijo.2012.1394
View abstract

Dyson OF; Walker LR; Whitehouse A; Cook PP; Akula SM Resveratrol Inhibits KSHV Reactivation by Lowering the Levels of Cellular EGR-1 PLOS ONE 7 -, 2012
DOI:10.1371/journal.pone.0033364

Hong Y; Macnab S; Lambert LA; Turner AJ; Whitehouse A; Usmani BA Herpesvirus saimiri-based endothelin-converting enzyme-1 shRNA expression decreases prostate cancer cell invasion and migration INT J CANCER 129 586-598, 2011
DOI:10.1002/ijc.25719

Turrell SJ; Whitehouse A Mutation of Herpesvirus Saimiri ORF51 Glycoprotein Specifically Targets Infectivity to Hepatocellular Carcinoma Cell Lines J BIOMED BIOTECHNOL -, 2011
DOI:10.1155/2011/785158

Tunnicliffe RB; Hautbergue GM; Kalra P; Jackson BR; Whitehouse A; Wilson SA; Golovanov AP Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57 PLOS PATHOG 7 -, 2011
DOI:10.1371/journal.ppat.1001244

Taylor A; Jackson BR; Noerenberg M; Hughes DJ; Boyne JR; Verow M; Harris M; Whitehouse A Mutation of a C-Terminal Motif Affects Kaposi's Sarcoma-Associated Herpesvirus ORF57 RNA Binding, Nuclear Trafficking, and Multimerization J VIROL 85 7881-7891, 2011
DOI:10.1128/JVI.00138-11

Khandelwal N; Simpson J; Taylor G; Rafique S; Whitehouse A; Hiscox J; Stark LA Nucleolar NF-kappa B/RelA mediates apoptosis by causing cytoplasmic relocalization of nucleophosmin CELL DEATH AND DIFFERENTIATION 18 1889-1903, 2011
DOI:10.1038/cdd.2011.79

Macnab SA; Turrell SJ; Carr IM; Markham AF; Coletta PL; Whitehouse A Herpesvirus saimiri-mediated delivery of the adenomatous polyposis coli tumour suppressor gene reduces proliferation of colorectal cancer cells. Int J Oncol 39 1173-1181, 2011
DOI:10.3892/ijo.2011.1130
View abstract

Jackson BR; Boyne JR; Noerenberg M; Taylor A; Hautbergue GM; Walsh MJ; Wheat R; Blackbourn DJ; Wilson SA; Whitehouse A An Interaction between KSHV ORF57 and UIF Provides mRNA-Adaptor Redundancy in Herpesvirus Intronless mRNA Export PLOS PATHOG 7 -, 2011
DOI:10.1371/journal.ppat.1002138

Boyne JR; Jackson BR; Taylor A; Macnab SA; Whitehouse A Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with PYM to enhance translation of viral intronless mRNAs EMBO J 29 1851-1864, 2010
DOI:10.1038/emboj.2010.77

Hiscox JA; Whitehouse A; Matthews DA Nucleolar proteomics and viral infection PROTEOMICS 10 4077-4086, 2010
DOI:10.1002/pmic.201000251

Boyne JR; Jackson BR; Whitehouse A ORF57 Master regulator of KSHV mRNA biogenesis CELL CYCLE 9 2702-2703, 2010
DOI:10.4161/cc.9.14.12627

Colgan KJ; Boyne JR; Whitehouse A Identification of a response element In a herpesvirus saimiri mRNA recognized by the ORF57 protein J GEN VIROL 90 596-601, 2009
DOI:10.1099/vir.0.007476-0

Colgan KJ; Boyne JR; Whitehouse A Uncoupling of hTREX demonstrates that UAP56 and hTHO-complex recruitment onto herpesvirus saimiri intronless transcripts is required for replication J GEN VIROL 90 1455-1460, 2009
DOI:10.1099/vir.0.010124-0

Gould F; Harrison SM; Hewitt EW; Whitehouse A Kaposi's Sarcoma-Associated Herpesvirus RTA Promotes Degradation of the Hey1 Repressor Protein through the Ubiquitin Proteasome Pathway J VIROL 83 6727-6738, 2009
DOI:10.1128/JVI.00351-09

Gao JY; Coulson JM; Whitehouse A; Blake N Reduction in RNA Levels Rather than Retardation of Translation Is Responsible for the Inhibition of Major Histocompatibility Complex Class I Antigen Presentation by the Glutamic Acid-Rich Repeat of Herpesvirus Saimiri Open Reading Frame 73 J VIROL 83 273-282, 2009
DOI:10.1128/JVI.01532-08

Macnab S; Whitehouse A Progress and prospects: human artificial chromosomes GENE THER 16 1180-1188, 2009
DOI:10.1038/gt.2009.102

Boyne JR; Whitehouse A Nucleolar disruption impairs Kaposi's sarcoma-associated herpesvirus ORF57-mediated nuclear export of intronless viral mRNAs FEBS LETT 583 3549-3556, 2009
DOI:10.1016/j.febslet.2009.10.040

Griffiths R; Harrison SM; Macnab S; Whitehouse A Mapping the minimal regions within the ORF73 protein required for herpesvirus saimiri episomal persistence J GEN VIROL 89 2843-2850, 2008
DOI:10.1099/vir.0.2008/002725-0

Emmott EP; Dove BK; Howell G; Chappell LA; Reed ML; Boyne JR; You JH; Brooks G; Whitehouse A; Hiscox JA Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus Virology 380 191-202, 2008
DOI:10.1016/j.virol.2008.05.032
View abstract

Harrison SM; Whitehouse A Kaposi's sarcoma-associated herpesvirus (KSHV) Rta and cellular HMGB1 proteins synergistically transactivate the KSHVORF50 promoter FEBS LETT 582 3080-3084, 2008
DOI:10.1016/j.febslet.2008.07.055

Macnab S; White R; Hiscox J; Whitehouse A Production of an infectious Herpesvirus saimiri-based episomally maintained amplicon system J BIOTECHNOL 134 287-296, 2008
DOI:10.1016/j.jbiotec.2008.01.012

Boyne JR; Colgan KJ; Whitehouse A Recruitment of the Complete hTREX Complex Is Required for Kaposi's Sarcoma-Associated Herpesvirus Intronless mRNA Nuclear Export and Virus Replication PLOS PATHOG 4 -, 2008
DOI:10.1371/journal.ppat.1000194

Boyne JR; Colgan KJ; Whitehouse A Herpesvirus saimiri ORF57: a post-transcriptional regulatory protein FRONT BIOSCI 13 2928-2938, 2008
DOI:10.2741/2898

Emmott E; Dove BK; Howell G; Chappell LA; Reed ML; Boyne JR; You JH; Brooks G; Whitehouse A; Hiscox JA Viral nucleolar localisation signals determine dynamic trafficking within the nucleolus VIROLOGY 380 191-202, 2008
DOI:10.1016/j.virol.2008.05.032

Wilson SJ; Tsao EH; Webb BLJ; Ye HT; Dalton-Griffin L; Tsantoulas C; Gale CV; Du MQ; Whitehouse A; Kellam P X box binding protein XBP-1s transactivates the Kaposi's sarcoma-associated herpesvirus (KSHV) ORF50 promoter, linking plasma cell differentiation to KSHV reactivation from latency J VIROL 81 13578-13586, 2007
DOI:10.1128/JVI.01663-07

Griffiths R; Whitehouse A Herpesvirus saimiri episomal persistence is maintained via interaction between open reading frame 73 and the cellular chromosome-associated protein MeCP2 J VIROL 81 4021-4032, 2007
DOI:10.1128/JVI.02171-06

Boyne JR; Whitehouse A gamma-2 Herpes virus post-transcriptional gene regulation CLIN MICROBIOL INFEC 12 110-117, 2006
DOI:10.1111/j.1469-0691.2005.01317.x

Boyne JR; Whitehouse A Nucleolar trafficking is essential for nuclear export of intronless herpesvirus mRNA P NATL ACAD SCI USA 103 15190-15195, 2006
DOI:10.1073/pnas.0604890103

Griffiths RA; Boyne JA; Whitehouse A Herpesvirus saimiri-based gene delivery vectors CURR GENE THER 6 1-15, 2006

Boyne JR; Whitehouse A γ-2 herpes virus post-transcriptional gene regulation Clinical Microbiology and Infection 12 110-117, 2006
DOI:10.1111/j.1469-0691.2005.01317.x
View abstract

Wakenshaw L; Walters MS; Whitehouse A The herpesvirus saimiri replication and transcription activator acts synergistically with CCAAT enhancer binding protein alpha to activate the DNA polymerase promoter J VIROL 79 13548-13560, 2005
DOI:10.1128/JVI.79.21.13548-13560.2005

Smith PG; Burchill SA; Brooke D; Coletta PL; Whitehouse A Efficient infection and persistence of a herpesvirus saimiri-based gene delivery vector into human tumor xenografts and multicellular spheroid cultures CANCER GENE THER 12 248-256, 2005
DOI:10.1038/sj.cgt.7700788

Calderwood M; White RE; Griffiths RA; Whitehouse A Open reading frame 73 is required for herpesvirus saimiri A11-S4 episomal persistence J GEN VIROL 86 2703-2708, 2005
DOI:10.1099/vir.0.81230-0

Williams BJL; Boyne JR; Goodwin DJ; Roaden L; Hautbergue GM; Wilson SA; Whitehouse A The prototype gamma-2 herpesvirus nucleocytoplasmic shuttling protein, ORF 57, transports viral RNA through the cellular mRNA export pathway BIOCHEM J 387 295-308, 2005
DOI:10.1042/BJ20041223

Walters MS; Hall KT; Whitehouse A The herpesvirus saimiri Rta gene autostimulates via binding to a non-consensus response element J GEN VIROL 86 581-587, 2005
DOI:10.1099/vir.0.80723-0

Smith PG; Oakley F; Fernandez M; Mann DA; Lemoine NR; Whitehouse A Herpesvirus saimiri-based vector biodistribution using noninvasive optical imaging GENE THER 12 1465-1476, 2005
DOI:10.1038/sj.gt.3302543

Calderwood NA; White RE; Whitehouse A Development of herpesvirus-based episomally maintained gene delivery vectors EXPERT OPIN BIOL TH 4 493-505, 2004

Walters MS; Hall KT; Whitehouse A The herpesvirus saimiri open reading frame (ORF) 50 (Rta) protein contains an AT hook required for binding to the ORF 50 response element in delayed-early promoters J VIROL 78 4936-4942, 2004
DOI:10.1128/JVI.78.9.4936-4942.2004

Calderwood MA; Hall KT; Matthews DA; Whitehouse A The herpesvirus saimiri ORF73 gene product interacts with host-cell mitotic chromosomes and self-associates via its C terminus J GEN VIROL 85 147-153, 2004
DOI:10.1099/vir.0.19437-0

Whitehouse A Herpesvirus saimiri: A potential gene delivery vector (Review) INT J MOL MED 11 139-148, 2003

Giles MS; Smith PG; Coletta PL; Hall KT; Whitehouse A The herpesvirus saimiri ORF 73 regulatory region provides long-term transgene expression in human carcinoma cell lines CANCER GENE THER 10 49-56, 2003

White RE; Calderwood MA; Whitehouse A Generation and precise modification of a herpesvirus salmiri bacterial artificial chromosome demonstrates that the terminal repeats are required for both virus production and episomal persistence J GEN VIROL 84 3393-3403, 2003
DOI:10.1099/vir.0.19387-0

Hall KT; Giles MS; Calderwood MA; Goodwin DJ; Matthews DA; Whitehouse A The herpesvirus saimiri open reading frame 73 gene product interacts with the cellular protein p32 J VIROL 76 11612-11622, 2002
DOI:10.1128/JVI.76.2211612-11612.2002

Goodwin DJ; Walters MS; Smith PG; Thurau M; Fickenscher H; Whitehouse A Herpesvirus saimiri open reading frame 50 (Rta) protein reactivates the lytic replication cycle in a persistently infected A549 cell line. J Virol 75 4008-4013, 2001
DOI:10.1128/JVI.75.8.4008-4013.2001
View abstract

Smith PG; Coletta PL; Markham AF; Whitehouse A In vivo episomal maintenance of a herpesvirus saimiri-based gene delivery vector. Gene Ther 8 1762-1769, 2001
DOI:10.1038/sj.gt.3301595
View abstract

Goodwin DJ; Whitehouse A A gamma-2 herpesvirus nucleocytoplasmic shuttle protein interacts with importin alpha 1 and alpha 5 J BIOL CHEM 276 19905-19912, 2001

Goodwin DJ; Whitehouse A Aγ-2 Herpesvirus Nucleocytoplasmic Shuttle Protein Interacts with Importin α1 and α5 Journal of Biological Chemistry 276 19905-19912, 2001
DOI:10.1074/jbc.M009513200
View abstract

Thurau M; Whitehouse A; Wittmann S; Meredith D; Fickenscher H Distinct transcriptional and functional properties of the R transactivator gene orf50 of the transforming herpesvirus Saimiri strain C488 VIROLOGY 268 167-177, 2000

Stevenson AJ; Clarke D; Meredith DM; Kinsey SE; Whitehouse A; Bonifer C Herpesvirus saimiri-based gene delivery vectors maintain heterologous expression throughout mouse embryonic stem cell differentiation in vitro GENE THER 7 464-471, 2000

Stevenson AJ; Frolova-Jones E; Hall KT; Kinsey SE; Markham AF; Whitehouse A; Meredith DM A herpesvirus saimiri-based gene therapy vector with potential for use in cancer immunotherapy CANCER GENE THER 7 1077-1085, 2000

Stevenson AJ; Giles MS; Hall KT; Goodwin DJ; Calderwood MA; Markham AF; Whitehouse A Specific oncolytic activity of herpesvirus saimiri in pancreatic cancer cells. Br J Cancer 83 329-332, 2000
DOI:10.1054/bjoc.2000.1346
View abstract

Hall KT; Giles MS; Goodwin DJ; Calderwood MA; Carr IM; Stevenson AJ; Markham AF; Whitehouse A Analysis of gene expression in a human cell line stably transduced with herpesvirus saimiri J VIROL 74 7331-7337, 2000

Goodwin DJ; Hall KT; Giles MS; Calderwood MA; Markham AF; Whitehouse A The carboxy terminus of the herpesvirus saimiri ORF 57 gene contains domains that are required for transactivation and transrepression J GEN VIROL 81 2253-2265, 2000

Hall KT; Giles MS; Goodwin DJ; Calderwood MA; Markham AF; Whitehouse A Characterization of the herpesvirus saimiri ORF73 gene product J GEN VIROL 81 2653-2658, 2000

Stevenson AJ; Cooper M; Griffiths JC; Gibson PC; Whitehouse A; Jones EF; Markham AF; Kinsey SE; Meredith DM Assessment of Herpesvirus saimiri as a potential human gene therapy vector J MED VIROL 57 269-277, 1999

Hall KT; Stevenson AJ; Goodwin DJ; Gibson PC; Markham AF; Whitehouse A The activation domain of herpesvirus saimiri R protein interacts with the TATA-binding protein J VIROL 73 9756-9763, 1999

Goodwin DJ; Hall KT; Stevenson AJ; Markham AF; Whitehouse A The open reading frame 57 gene product of herpesvirus saimiri shuttles between the nucleus and cytoplasm and is involved in viral RNA nuclear export J VIROL 73 10519-10524, 1999

Cooper M; Goodwin DJ; Hall KT; Stevenson AJ; Meredith DM; Markham AF; Whitehouse A The gene product encoded by ORF 57 of herpesvirus saimiri regulates the redistribution of the splicing factor SC-35 J GEN VIROL 80 1311-1316, 1999

Bello LJ; Davison AJ; Glenn MA; Whitehouse A; Rethmeier N; Schulz TF; Clements JB The human herpesvirus-8 ORF 57 gene and its properties J GEN VIROL 80 3207-3215, 1999

Whitehouse A; Cooper M; Meredith DM The immediate-early gene product encoded by open reading frame 57 of herpesvirus saimiri modulates gene expression at a posttranscriptional level J VIROL 72 857-861, 1998

Whitehouse A; Cooper M; Hall KT; Meredith DM The open reading frame (ORF) 50a gene product regulates ORF 57 gene expression in herpesvirus saimiri J VIROL 72 1967-1973, 1998

Carr IM; Whitehouse A; Coletta PL; Markham AF Structural and evolutionary characterization of the human sorbitol dehydrogenase gene duplication MAMM GENOME 9 1042-1048, 1998

Whitehouse A; Meredith DM; Markham AF DNA mismatch repair genes and their association with colorectal cancer (Review) INT J MOL MED 1 469-474, 1998

Whitehouse A; Deeble J; Parmar R; Taylor GR; Markham AF; Meredith DM Analysis of the mismatch and insertion/deletion binding properties of Thermus thermophilus, HB8, MutS BIOCHEM BIOPH RES CO 233 834-837, 1997

Whitehouse A; Deeble J; Taylor GR; Guillou PJ; Phillips SEV; Meredith DM; Markham AF Mapping the minimal domain of hMSH-2 sufficient for binding mismatched oligonucleotides BIOCHEM BIOPH RES CO 232 10-13, 1997

Whitehouse A; Stevenson AJ; Cooper M; Meredith DM Identification of a cis-acting element within the herpesvirus saimiri ORF 6 promoter that is responsive to the HVS.R transactivator J GEN VIROL 78 1411-1415, 1997

Deeble JV; Whitehouse A; Roberts E; Woods CG; Meredith D; Markham AF; Taylor GR Evaluation of enzymatic methods of mutation detection. AM J HUM GENET 61 A219-A219, 1997

Deeble J; Whitehouse A; Meredith DM; Markham AF; Woods CG; Taylor GR Use of Thermus thermophilus HB8 MutS in a filter binding format for mismatch detection. J MED GENET 34 1306-1306, 1997

Whitehouse A; Carr IM; Griffiths JC; Meredith DM The herpesvirus Saimiri ORF50 gene, encoding a transcriptional activator homologous to the Epstein-Barr virus R protein, is transcribed from two distinct promoters of different temporal phases J VIROL 71 2550-2554, 1997

Norris AL; Bailey A; Askham J; Whitehouse A; Clissold PM; Markham AF; Meredith DM Characterization of the human beta-catenin gene MAMM GENOME 7 160-162, 1996

Whitehouse A; Parmar R; Deeble J; Taylor GR; Phillips SEV; Meredith DM; Markham AF Mutational analysis of the nucleotide binding domain of the mismatch repair enzyme hMSH-2 BIOCHEM BIOPH RES CO 229 147-153, 1996

Whitehouse A; Taylor GR; Deeble J; Phillips SEV; Meredith DM; Markham AF A carboxy terminal domain of the hMSH-2 gene product is sufficient for binding specific mismatched oligonucleotides BIOCHEM BIOPH RES CO 225 289-295, 1996