Faculty of Biological Sciences

Prof Peter Urwin

BSc, Coventry; PhD 1992, Durham.
Professor of Plant Nematology
School of Biology

Contact:  Manton 9.10, +44(0) 113 34 32909 office/33035 lab, email address for  

You can read more about Prof Urwin's interests here:
http://www.fbs.leeds.ac.uk/nem/

Research Interests

Devloping and deploying defenses against plant parasitic nematodes

Transgenic Potato Field TrialCysts on RootsMeloidogyne Section

Plant parasitic nematodes (PPNs) cause >$100 billion annual losses to world agriculture of which cyst and root-knot nematodes contribute over 80%. In the lab we are developing biofumigation to replace withdrawn nematicides and utilising well established anti-feedant technology as well as newly developed behavioural repellents and RNAi to provide plant based resistance to nematodes in several crops. We are also undertaking fundamental research using the model species C. elegans and utilising the data being generated by the sequencing of Globodera pallida to identify novel strategies and targets for engineering nematode resistance.

 

Current Projects

  • Establishing biofumigation as a sustainable pesticide replacement for control of soil-borne pests and pathogens in potato and horticultural crops.  
  • Effects of environmental change: increased nematode pest status on UK crops.  
  • Biotic constraints to crop production: biochemical and molecular basis of plant interactions with pests, parasites and symbionts.  
  • Development of nematode resistant cotton by genetic engineering.  
  • Characterisation of cue-dependent behaviour in plant parasitic nematodes (PPNs); the neurobiology of host plant invasion.  
  • Development of nematode resistant cooking banana by genetic engineering for sub-Saharan Africa.

 

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Plant nematology

Postgraduate studentship areas:

  • Understanding plant-nematode interactions, developing novel anti-nematode defenses

See also:

Modules managed

BLGY2225 - Sustainable Food Production
BLGY3205 - Applied Plant Science

Modules taught

BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5392M - Bioscience MSc Research Project
BLGY1211 - Applied Biology and Agriculture
BLGY1236 - Practical Applied Biology
BLGY1303 - Tutorials for Biology and Genetics
BLGY2100 - Enhanced Study Skills for Biologists
BLGY2225 - Sustainable Food Production
BLGY2253 - Animals as Pests
BLGY3021 - Research Project
BLGY3205 - Applied Plant Science
BLGY3340 - Biology Research Projects

Committees

Member of Undergraduate School Taught Student Education Committee (Programme Manager: Applied Biology)

Centre membership: The Centre for Plant Sciences

Mrs Jennie Hibbard  (Research Technician)

Dr Laura Jones  (Research Fellow)

Dr Catherine Lilley  (Senior Research Fellow)

Mrs Fiona Moulton  (Research Technician)

Dr Hugh Roderick  (Research Fellow)

Nematode Resistant Cooking Bananas for sub-Saharan Africa 


Postgraduates

Christopher Bell  
Refik Bozbuga  
Duqing Wu  
Grace Hoysted  
John Lennon  
Jessica Marvin  
Li Zhang [RPG]  

Eves-van den Akker S, Laetsch DR, Thorpe P, Lilley CJ, Danchin EGJ, Da Rocha M, Rancurel C, Holroyd NE, Cotton JA, Szitenberg A, Grenier E, Montarry J, Mimee B, Duceppe MO, Boyes I, Marvin JMC, Jones LM, Yusup HB, Lafond-Lapalme J, Esquibet M, Sabeh M, Rott M, Overmars H, Finkers-Tomczak A, Smant G, Koutsovoulos G, Blok V, Mantelin S, Cock PJA, Phillips W, Henrissat B, Urwin PE, Blaxter M, Jones JT The genome of the yellow potato cyst nematode, Globodera rostochiensis, reveals insights into the basis of parasitism and virulence Genome Biology 17 -, 2016
DOI:10.1186/s13059-016-0985-1
View abstract

Eves-van den Akker S, Lilley CJ, Yusup HB, Jones JT, Urwin PE Functional C-terminally encoded plant peptide (CEP) hormone domains evolved de novo in the plant parasite Rotylenchulus reniformis. Molecular plant pathology -, 2016
DOI:10.1111/mpp.12402
View abstract

Holden-Dye L, Kearn J, Crisford A, Ludlow E, Urwin PE, Lilley CJ, O'Connor V THE NEMATICIDAL MODE OF ACTION OF FLUENSULFONE., 2015

Eves-van den Akker S, Lilley CJ, Danchin EGJ, Rancurel C, Cock PJA, Jones LM, Yusup HB, Jones JT, Urwin PE COMPARATIVE GENOMICS/TRANSCRIPTOMICS TO STUDY EFFECTOR GENE BIRTH IN PLANT-PARASITIC NEMATODES., 2015

Jones LM, Flemming AJ, Urwin PE NHR-176 regulates cyp-35d1 to control hydroxylation-dependent metabolism of thiabendazole in caenorhabditis elegans Biochemical Journal 466 37-44, 2015
DOI:10.1042/BJ20141296
View abstract

Eves-van den Akker S, Lilley CJ, Jones JT, Urwin PE Plant-parasitic nematode feeding tubes and plugs: new perspectives on function NEMATOLOGY 17 1-9, 2015
DOI:10.1163/15685411-00002832

Lilley CJ, Jain R, Urwin PE Reduction of phytate by down-regulation of Arabidopsis thaliana MIPS and IPK1 genes alters susceptibility to beet cyst nematodes Nematology 17 401-407, 2015
DOI:10.1163/15685411-00002874
View abstract

Eves-Van Den Akker S, Lilley CJ, Reid A, Pickup J, Anderson E, Cock PJA, Blaxter M, Urwin PE, Jones JT, Blok VC A metagenetic approach to determine the diversity and distribution of cyst nematodes at the level of the country, the field and the individual Molecular Ecology 24 5842-5851, 2015
DOI:10.1111/mec.13434
View abstract

Atkinson NJ, Jain R, Urwin PE The response of plants to simultaneous biotic and abiotic stress In Combined Stresses in Plants: Physiological, Molecular, and Biochemical Aspects , 2015
DOI:10.1007/978-3-319-07899-1_9
View abstract

Tripathi L, Babirye A, Roderick H, Tripathi JN, Changa C, Urwin PE, Tushemereirwe WK, Coyne D, Atkinson HJ Field resistance of transgenic plantain to nematodes has potential for future African food security. Sci Rep 5 8127-, 2015
DOI:10.1038/srep08127
View abstract

Thorpe P, Mantelin S, Cock PJ, Blok VC, Coke MC, Eves-van den Akker S, Guzeeva E, Lilley CJ, Smant G, Reid AJ, Wright KM, Urwin PE, Jones JT Genomic characterisation of the effector complement of the potato cyst nematode Globodera pallida BMC GENOMICS 15 -, 2014
DOI:10.1186/1471-2164-15-923

Eves-van den Akker S, Lilley CJ, Danchin EG, Rancurel C, Cock PJ, Urwin PE, Jones JT The transcriptome of Nacobbus aberrans reveals insights into the evolution of sedentary endoparasitism in plant-parasitic nematodes. Genome Biol Evol 6 2181-2194, 2014
DOI:10.1093/gbe/evu171
View abstract

Eves-van den Akker S, Lilley CJ, Jones JT, Urwin PE Identification and characterisation of a hyper-variable apoplastic effector gene family of the potato cyst nematodes. PLoS Pathog 10 e1004391-, 2014
DOI:10.1371/journal.ppat.1004391
View abstract

Cotton JA, Lilley CJ, Jones LM, Kikuchi T, Reid AJ, Thorpe P, Tsai IJ, Beasley H, Blok V, Cock PJ, Eves-van den Akker S, Holroyd N, Hunt M, Mantelin S, Naghra H, Pain A, Palomares-Rius JE, Zarowiecki M, Berriman M, Jones JT, Urwin PE The genome and life-stage specific transcriptomes of Globodera pallida elucidate key aspects of plant parasitism by a cyst nematode. Genome Biol 15 R43-, 2014
DOI:10.1186/gb-2014-15-3-r43
View abstract

Eves-van den Akker S, Lilley CJ, Ault JR, Ashcroft AE, Jones JT, Urwin PE The feeding tube of cyst nematodes: characterisation of protein exclusion. PLoS One 9 e87289-, 2014
DOI:10.1371/journal.pone.0087289
View abstract

Crisford A, Ludlow E, Marvin J, Kearn J, O'Connor V, Urwin PE, Lilley C, Holden-Dye L LEVERAGING C. ELEGANS CUE-DEPENDENT BEHAVIOR TO UNDERSTAND THE HOST/PARASITE INTERACTION FOR PLANT PARASITIC NEMATODES JOURNAL OF NEMATOLOGY 45 285-285, 2013

Atkinson NJ, Lilley CJ, Urwin PE Identification of Genes Involved in the Response of Arabidopsis to Simultaneous Biotic and Abiotic Stresses PLANT PHYSIOLOGY 162 2028-2041, 2013
DOI:10.1104/pp.113.222372

Jones LM, Rayson SJ, Flemming AJ, Urwin PE Adaptive and specialised transcriptional responses to xenobiotic stress in Caenorhabditis elegans are regulated by nuclear hormone receptors. PLoS One 8 e69956-, 2013
DOI:10.1371/journal.pone.0069956
View abstract

Davies LJ, Urwin PE The cell walls of syncytia formed by Heterodera schachtii in Arabidopsis thaliana are abundant in methyl-esterified pectin. Plant Signal Behav 7 1404-1406, 2012
DOI:10.4161/psb.21925
View abstract

Davies LJ, Lilley CJ, Paul Knox J, Urwin PE Syncytia formed by adult female Heterodera schachtii in Arabidopsis thaliana roots have a distinct cell wall molecular architecture New Phytologist 196 238-246, 2012
DOI:10.1111/j.1469-8137.2012.04238.x
View abstract

Roderick H, Tripathi L, Babirye A, Wang D, Tripathi J, Urwin PE, Atkinson HJ Generation of transgenic plantain (Musa spp.) with resistance to plant pathogenic nematodes. Mol Plant Pathol 13 842-851, 2012
DOI:10.1111/j.1364-3703.2012.00792.x
View abstract

Atkinson NJ, Urwin PE The interaction of plant biotic and abiotic stresses: from genes to the field. J Exp Bot 63 3523-3543, 2012
DOI:10.1093/jxb/ers100
View abstract

Atkinson HJ, Lilley CJ, Urwin PE Strategies for transgenic nematode control in developed and developing world crops. Curr Opin Biotechnol 23 251-256, 2012
DOI:10.1016/j.copbio.2011.09.004
View abstract

Lilley CJ, Davies LJ, Urwin PE RNA interference in plant parasitic nematodes: a summary of the current status. Parasitology 139 630-640, 2012
DOI:10.1017/S0031182011002071
View abstract

Green J, Wang D, Lilley CJ, Urwin PE, Atkinson HJ Transgenic potatoes for potato cyst nematode control can replace pesticide use without impact on soil quality. PLoS One 7 e30973-, 2012
DOI:10.1371/journal.pone.0030973
View abstract

Atkinson NJ, Dew TP, Orfila C, Urwin PE Influence of combined biotic and abiotic stress on nutritional quality parameters in tomato (Solanum lycopersicum). J Agric Food Chem 59 9673-9682, 2011
DOI:10.1021/jf202081t
View abstract

Lord JS, Lazzeri L, Atkinson HJ, Urwin PE Biofumigation for Control of Pale Potato Cyst Nematodes: Activity of Brassica Leaf Extracts and Green Manures on Globodera pallida in Vitro and in Soil J AGR FOOD CHEM 59 7882-7890, 2011
DOI:10.1021/jf200925k

Wang D, Jones LM, Urwin PE, Atkinson HJ A Synthetic Peptide Shows Retro- and Anterograde Neuronal Transport before Disrupting the Chemosensation of Plant-Pathogenic Nematodes PLOS ONE 6 -, 2011
DOI:10.1371/journal.pone.0017475

Lilley CJ, Wang D, Atkinson HJ, Urwin PE Effective delivery of a nematode-repellent peptide using a root-cap-specific promoter PLANT BIOTECHNOL J 9 151-161, 2011
DOI:10.1111/j.1467-7652.2010.00542.x

Atkinson HJ, Urwin PE, Hussey RS Plant biotechnology and control, 2009

Costa JC, Lilley CJ, Atkinson HJ, Urwin PE Functional characterisation of a cyst nematode acetylcholinesterase gene using Caenorhabditis elegans as a heterologous system INT J PARASITOL 39 849-858, 2009
DOI:10.1016/j.ijpara.2008.12.007

Siddique S, Endres S, Atkins JM, Szakasits D, Wieczorek K, Hofmann J, Blaukopf C, Urwin PE, Tenhaken R, Grundler FMW, Kreil DP, Bohlmann H Myo-inositol oxygenase genes are involved in the development of syncytia induced by Heterodera schachtii in Arabidopsis roots NEW PHYTOL 184 457-472, 2009
DOI:10.1111/j.1469-8137.2009.02981.x

Bakhetia M, Urwin PE, Atkinson HJ Characterisation by RNAi of pioneer genes expressed in the dorsal pharyngeal gland cell of Heterodera glycines and the effects of combinatorial RNAi INT J PARASITOL 38 1589-1597, 2008
DOI:10.1016/j.ijpara.2008.05.003

Fuller VL, Lilley CJ, Urwin PE Nematode resistance NEW PHYTOL 180 27-44, 2008
DOI:10.1111/j.1469-8137.2008.02508.x

Atkinson HJ, Urwin PE Europe needs to protect its transgenic crop research NATURE 453 979-979, 2008
DOI:10.1038/453979c

Fuller VL, Lilley CJ, Atkinson HJ, Urwin PE Differential gene expression in Arabidopsis following infection by plant-parasitic nematodes Meloidogyne incognita and Heterodera schachtii MOL PLANT PATHOL 8 595-609, 2007
DOI:10.1111/J.1364-3703.2007.00416.X

Lilley CJ, Bakhetia M, Charlton WL, Urwin PE Recent progress in the development of RNA interference for plant parasitic nematodes MOL PLANT PATHOL 8 701-711, 2007
DOI:10.1111/J.1364-3703.2007.00422.X

Urwin PE Optimizing biotech crops for nematode resistance. PHYTOPATHOLOGY 97 S144-S144, 2007

Bakhetia M, Urwin PE, Atkinson HJ qPCR analysis and RNAi define pharyngeal gland cell-expressed genes of Heterodera glycines required for initial interactions with the host MOL PLANT MICROBE IN 20 306-312, 2007
DOI:10.1094/MPMI-20-3-0306

Shingles J, Lilley CJ, Atkinson HJ, Urwin PE Meloidogyne incognita: Molecular and biochemical characterisation of a cathepsin L cysteine proteinase and the effect on parasitism following RNAi EXP PARASITOL 115 114-120, 2007
DOI:10.1016/j.exppara.2006.07.008

Costa JC, Lilley CJ, Urwin PE Caenorhabditis elegans as a model for plant-parasitic nematodes NEMATOLOGY 9 3-16, 2007

Lilley CJ, Goodchild SA, Atkinson HJ, Urwin PE Cloning and characterisation of a Heterodera glycines aminopeptidase cDNA INT J PARASITOL 35 1577-1585, 2005
DOI:10.1016/j.ijpara.2005.07.017

Lilley CJ, Atkinson HJ, Urwin PE Molecular aspects of cyst nematodes MOL PLANT PATHOL 6 577-588, 2005
DOI:10.1111/J.1364-3703.2005.00306.X

Liu B, Hibbard JK, Urwin PE, Atkinson HJ The production of synthetic chemodisruptive peptides in planta disrupts the establishment of cyst nematodes PLANT BIOTECHNOL J 3 487-496, 2005
DOI:10.1111/j.1467-7652.2005.00139.x

Bakhetia M, Charlton WL, Urwin PE, McPherson MJ, Atkinson HJ RNA interference and plant parasitic nematodes TRENDS PLANT SCI 10 362-367, 2005
DOI:10.1016/j.tplants.2005.06.007

Lilley CJ, Urwin PE, Johnston KA, Atkinson HJ Preferential expression of a plant cystatin at nematode feeding sites confers resistance to Meloidogyne incognita and Globodera pallida PLANT BIOTECHNOL J 2 3-12, 2004
DOI:10.1046/j.1467-7652.2003.00037.x

Urwin PE, Green J, Atkinson HJ Expression of a plant cystatin confers partial resistance to Globodera, full resistance is achieved by pyramiding a cystatin with natural resistance MOL BREEDING 12 263-269, 2003

Atkinson HJ, Urwin PE, McPherson MJ Engineering plants for nematode resistance. Annu Rev Phytopathol 41 615-639, 2003
DOI:10.1146/annurev.phyto.41.052002.095737
View abstract

Urwin PE, Zubko EI, Atkinson HJ The biotechnological application and limitation of IRES to deliver multiple defence genes to plant pathogens Physiological and Molecular Plant Pathology 61 103-108, 2002
DOI:10.1016/S0885-5765(02)90421-2
View abstract

Urwin PE, Lilley CJ, Atkinson HJ Ingestion of double-stranded RNA by preparasitic juvenile cyst nematodes leads to RNA interference. Mol Plant Microbe Interact 15 747-752, 2002
DOI:10.1094/MPMI.2002.15.8.747
View abstract

Urwin PE, Troth KM, Zubko EI, Atkinson HJ Effective transgenic resistance to Globodera pallida in potato field trials MOL BREEDING 8 95-101, 2001

Urwin PE, Yi L, Martin H, Atkinson HJ, Gilmartin PM Functional characterization of the EMCV IRES in plants The Plant Journal 24 583-589, 2000
View abstract

Urwin PE, Green J, Atkinson HJ Resistance to Globodera spp. in transgenic Solanum tuberosum cv. Desiree that express proteinase inhibitors. In Aspects of Applied Biology , 2000

Urwin PE, Levesley A, McPherson MJ, Atkinson HJ Transgenic resistance to the nematode Rotylenchulus reniformis conferred by A. thaliana plants expressing proteinase inhibitors Molecular Breeding 6 257-264, 2000

Walker AJ, Urwin PE, Atkinson HJ, Brain P, Glen DM, Shewry PR Transgenic Arabidopsis leaf tissue expressing a modified oryzacystatin shows resistance to the field slug Deroceras reticulatum (Muller) TRANSGENIC RES 8 95-103, 1999

Lilley CJ, Urwin PE, Atkinson HJ Characterization of plant nematode genes: identifying targets for a transgenic defence. Parasitology 118 Suppl S63-S72, 1999
View abstract

Urwin PE, Møller SG, Blumsom JK, Atkinson HJ Continual green fluorescent protein monitoring of promoter activity in plants. Methods Enzymol 302 316-328, 1999

Lilley CJ, Devlin P, Urwin PE, Atkinson HJ Parasitic nematodes, Proteinases and transgenic plants Parasitology Today 15 414-417, 1999

Urwin PE, Lilley CJ Resistance to plant parasitic nematodes conferred by GM-crops. Pesticide Outlook 10 145-149, 1999

Urwin PE, Moller SG, Blumsom JK, Atkinson HJ Continual monitoring of promoter activity in plants Methods in Enzymology 302 316-328, 1999

Roman R, Urwin PE, Atkinson HJ, Gilmartin PM Hgm1, a novel male-specific gene from Heterodera glycines identified by differential mRNA display Molecular and Biochemical Parasitology 95 45-52, 1998
View abstract

Moller SG, Urwin PE, Atkinson HJ, McPherson MJ Nematode-induced expression of atao1, a gene encoding an extracellular diamine oxidase associated with developing vascular tissue PHYSIOL MOL PLANT P 53 73-79, 1998

Urwin PE, McPherson MJ, Atkinson HJ Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs PLANTA 204 472-479, 1998

Lilley CJ, Urwin PE, Atkinson HJ, McPherson MJ Characterisation of cDNAs encoding serine proteinases from the soybean cyst nematode Molecular and Biochemical Parasitology 89 195-207, 1997
View abstract

Urwin PE, Lilley CJ, McPherson MJ, Atkinson HJ Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin PLANT J 12 455-461, 1997

Urwin PE, Lilley CJ, McPherson MJ, Atkinson HJ Characterization of two cDNAs encoding cysteine proteinases from the soybean cyst nematode Heterodera glycines PARASITOLOGY 114 605-613, 1997

Urwin PE, Møller SG, Lilley CJ, McPherson MJ, Atkinson HJ Continual green-fluorescent protein monitoring of cauliflower mosaic virus 35S promoter activity in nematode-induced feeding cells in Arabidopsis thaliana. Mol Plant Microbe Interact 10 394-400, 1997
DOI:10.1094/MPMI.1997.10.3.394
View abstract

Urwin PE, Moller SG, Lilley CJ, Atkinson HJ, McPherson MJ Continual GFP monitoring of CaMV35S promoter activity in nematode induced feeding cells in Arabidopsis thaliana Molecular Plant-Microbe Interactions 10 394-400, 1997

Atkinson HJ, Urwin PE, Lilley CJ, McPherson MJ Engineering transgenic resistance to potato cyst nematodes In Potato Cyst Nematodes , 1997

Fordam-skelton AP, Lilley CJ, Urwin PE, Robinson NJ GUS expression in Arabidopsis directed by the 5' regions of the pea metallothionein-like gene PsMTa Plant Molecular Biology 34 659-668, 1997

Urwin PE, Groom QJ, Robinson NJ Characterization of two cDNAs and identification of two proteins that accumulate in response to cadmium in cadmium-tolerant Datura innoxia (Mill.) cells Journal of Experimental Botany 47 1019-1024, 1996
View abstract

Lilley CJ, Urwin PE, McPherson MJ, Atkinson HJ Characterization of intestinally active proteinases of cyst-nematodes PARASITOLOGY 113 415-424, 1996

Atkinson HJ, Urwin PE, Clarke MC, McPherson MJ Image analysis of the growth of Globodera pallida and Meloidogyne incognita on transgenic tomato roots expressing cystatins J NEMATOL 28 209-215, 1996

Urwin PE, Atkinson HJ, McPherson MJ Involvement of the NH2-terminal region of oryzacystatin-I in cysteine proteinase inhibition. Protein Eng 8 1303-1307, 1995
View abstract

ATKINSON HJ, URWIN PE, HANSEN E, MCPHERSON MJ DESIGNS FOR ENGINEERED RESISTANCE TO ROOT-PARASITIC NEMATODES TRENDS BIOTECHNOL 13 369-374, 1995

Urwin PE, Atkinson HJ, Waller DA, McPherson MJ Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant J 8 121-131, 1995
View abstract

Tommey AM, Shi J, Lindsay WP, Urwin PE, Robinson NJ Expression of the pea gene PSMTA in E. coli. Metal-binding properties of the expressed protein. FEBS Lett 292 48-52, 1991
View abstract

Papolu PK, Dutta TK, Tyagi N, Urwin PE, Lilley CJ, Rao U Expression of a cystatin transgene in eggplant provides resistance to root-knot nematode, Meloidogyne incognita Frontiers in Plant Science -,
View abstract