Faculty of Biological Sciences

Dr Roman Tuma

Ph.D. 1996
Reader in Structural Biology
School of Molecular and Cellular Biology

Contact:  Miall 10.27, +44(0) 113 34 33080, email address for  

You can read more about Dr Tuma's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=RT

Research Interests

macromolecular assembly and dynamics, molecular motors, single molecule biophysics

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • 1. Catalytic cooperativity of hexameric helicases by site-directed mutagenesis
  • 2. dsRNA virus assembly in vitro
  • 3. Molecular motors in chromatin remodelling - domain structure and interactions

Postgraduate studentship areas:

  • 1. Single molecule studies of virus assembly
  • 2. Mechano-chemical coupling in molecular motors that translocate along nucleic acids
  • 3. Assembly of bacteriochlorophylls on solid surfaces

See also:

Modules managed

BIOC2302 - Intermediate Biochemistry: Practicals

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC1303 - Introductory Biochemistry: Problem Solving and Data Handling
BIOC2301 - Intermediate Integrated Biochemistry
BIOC2302 - Intermediate Biochemistry: Practicals
BIOC2303 - Intermediate Biochemistry: Skills
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOL2111/BIOC2301 - Integrated Biochemistry/Genetic Engineering
BIOL2301/03/05 - Skills for Biol Sci & Biosciences
BIOL3398 - Research Tools and Applications
BIOL3399 - Extended Research Project Preparation
BIOL5371M - Research Planning and Scientific Communication
BIOW5901X - Foundation module
BIOW5905X - Membrane proteins

Centre membership: The Astbury Centre for Structural Molecular Biology

Postgraduates

Jack Bravo (Primary supervisor) 50% FTE
Xiaoling Wang (Co-supervisor) 10% FTE

Li M, Khan S, Rong H, Tuma R, Hatzakis NS, Jeuken LJC Effects of membrane curvature and pH on proton pumping activity of single cytochrome bo<inf>3</inf>enzymes Biochimica et Biophysica Acta - Bioenergetics 1858 763-770, 2017
DOI:10.1016/j.bbabio.2017.06.003
View abstract

Patel N, White SJ, Thompson RF, Bingham R, Weiß EU, Maskell DP, Zlotnick A, Dykeman EC, Tuma R, Twarock R, Ranson NA, Stockley PG HBV RNA pre-genome encodes specific motifs that mediate interactions with the viral core protein that promote nucleocapsid assembly Nature Microbiology 2 -, 2017
DOI:10.1038/nmicrobiol.2017.98
View abstract

Dobson J, Kumar A, Willis LF, Tuma R, Higazi DR, Turner R, Lowe DC, Ashcroft AE, Radford SE, Kapur N, Brockwell DJ Inducing protein aggregation by extensional flow Proceedings of the National Academy of Sciences of the United States of America 114 4673-4678, 2017
DOI:10.1073/pnas.1702724114
View abstract

Borodavka A, Singaram SW, Stockley PG, Gelbart WM, Ben-Shaul A, Tuma R Sizes of Long RNA Molecules Are Determined by the Branching Patterns of Their Secondary Structures Biophysical Journal 111 2077-2085, 2016
DOI:10.1016/j.bpj.2016.10.014
View abstract

Morra R, Fessl T, Wang Y, Mancini EJ, Tuma R Biophysical characterization of chromatin remodeling protein CHD4 In Chromosome Architecture: Methods and Protocols , 2016
DOI:10.1007/978-1-4939-3631-1_14
View abstract

Allen WJ, Corey RA, Oatley P, Sessions RB, Baldwin SA, Radford SE, Tuma R, Collinson I Two-way communication between SecY and SecA suggests a brownian ratchet mechanism for protein translocation eLife 5 -, 2016
DOI:10.7554/eLife.15598
View abstract

Stockley PG, White SJ, Dykeman E, Manfield I, Rolfsson O, Patel N, Bingham R, Barker A, Wroblewski E, Chandler-Bostock R, Weiß EU, Ranson NA, Tuma R, Twarock R Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid. Bacteriophage 6 e1157666-, 2016
DOI:10.1080/21597081.2016.1157666
View abstract

Li M, Jørgensen SK, McMillan DGG, Krzemiński Ł, Daskalakis NN, Partanen RH, Tutkus M, Tuma R, Stamou D, Hatzakis NS, Jeuken LJC Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase, 2015
DOI:10.1021/jacs.5b08798
View abstract

Li M, Jorgensen SK, McMillan DGG, Krzemniski L, Daskalakis NN, Partanen RH, Tutkus M, Tuma R, Stamou D, Hatzakis NS, Jeuken LJC Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 137 16055-16063, 2015
DOI:10.1021/jacs.5b08798

Zhang N, Schäfer J, Sharma A, Rayner L, Zhang X, Tuma R, Stockley P, Buck M Mutations in RNA Polymerase Bridge Helix and Switch Regions Affect Active-Site Networks and Transcript-Assisted Hydrolysis Journal of Molecular Biology 427 3516-3526, 2015
DOI:10.1016/j.jmb.2015.09.005
View abstract

Tipping KW, Karamanos TK, Jakhria T, Iadanza MG, Goodchild SC, Tuma R, Ranson NA, Hewitt EW, Radford SE pH-induced molecular shedding drives the formation of amyloid fibril-derived oligomers Proceedings of the National Academy of Sciences of the United States of America 112 E5691-E5696, 2015
DOI:10.1073/pnas.1423174112
View abstract

Patel N, Dykeman EC, Coutts RHA, Lomonossoff GP, Rowlands DJ, Phillips SEV, Ranson N, Twarock R, Tuma R, Stockley PG Revealing the density of encoded functions in a viral RNA. Proc Natl Acad Sci U S A 112 2227-2232, 2015
DOI:10.1073/pnas.1420812112
View abstract

Borodavka A, Ault J, Stockley PG, Tuma R Evidence that avian reovirusσnS is an RNA chaperone: Implications for genome segment assortment Nucleic Acids Research 43 7044-7057, 2015
DOI:10.1093/nar/gkv639
View abstract

Radou G, Dreyer FN, Tuma R, Paci E Functional dynamics of hexameric helicase probed by hydrogen exchange and simulation Biophysical Journal 107 983-990, 2014
DOI:10.1016/j.bpj.2014.06.039
View abstract

Sharma A, Leach RN, Gell C, Zhang N, Burrows PC, Shepherd DA, Wigneshweraraj S, Smith DA, Zhang X, Buck M, Stockley PG, Tuma R Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies. Nucleic Acids Res 42 5177-5190, 2014
DOI:10.1093/nar/gku146
View abstract

Scott JNF, Kupinski AP, Kirkham CM, Tuma R, Boyes J TALE proteins bind to both active and inactive chromatin. Biochem J 458 153-158, 2014
DOI:10.1042/BJ20131327
View abstract

Psencik J, Butcher SJ, Tuma R Chlorosomes: Structure, Function and Assembly STRUCTURAL BASIS OF BIOLOGICAL ENERGY GENERATION 39 77-109, 2014
DOI:10.1007/978-94-017-8742-0_5

Borodavka A, Tuma R, Stockley PG A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence RNA Biology 10 481-489, 2013
DOI:10.4161/rna.23838
View abstract

Pšenčík J, Arellano JB, Collins AM, Laurinmäki P, Torkkeli M, Löflund B, Serimaa RE, Blankenship RE, Tuma R, Butcher SJ Structural and functional roles of carotenoids in chlorosomes Journal of Bacteriology 195 1727-1734, 2013
DOI:10.1128/JB.02052-12
View abstract

El Omari K, Meier C, Kainov D, Sutton G, Grimes JM, Poranen MM, Bamford DH, Tuma R, Stuart DI, Mancini EJ Tracking in atomic detail the functional specializations in viral RecA helicases that occur during evolution Nucleic Acids Research 41 9396-9410, 2013
DOI:10.1093/nar/gkt713
View abstract

Stockley PG, Barker AM, Borodavka A, Ford RJ, Pearson AR, Ranson NA, Tuma R, Twarock R, Dykeman E, Phillips SEV, Bakker SE Packaging signals in single-stranded RNA viruses: nature's alternative to a purely electrostatic assembly mechanism Journal of Biological Physics 1-11, 2013
DOI:10.1007/s10867-013-9313-0
View abstract

Alster J, Kabeláč M, Tuma R, Pšenčík J, Burda JV Computational study of short-range interactions in bacteriochlorophyll aggregates Computational and Theoretical Chemistry 998 87-97, 2012
DOI:10.1016/j.comptc.2012.07.001
View abstract

Borodavka A, Tuma R, Stockley PG Evidence that viral RNAs have evolved for efficient, two-stage packaging PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 109 15769-15774, 2012
DOI:10.1073/pnas.1204357109

Morra R, Lee BM, Shaw H, Tuma R, Mancini EJ Concerted action of the PHD, chromo and motor domains regulates the human chromatin remodelling ATPase CHD4 FEBS LETTERS 586 2513-2521, 2012
DOI:10.1016/j.febslet.2012.06.017

Mancini EJ, Tuma R Mechanism of RNA packaging motor Advances in Experimental Medicine and Biology 726 609-629, 2012
DOI:10.1007/978-1-4614-0980-9_27
View abstract

Rabhi M, Tuma R, Boudvillain M RNA remodeling by hexameric RNA helicases RNA BIOL 7 655-666, 2010
DOI:10.4161/rna.7.6.13570

Psencik J, Torkkeli M, Zupcanova A, Vacha F, Serimaa RE, Tuma R The lamellar spacing in self-assembling bacteriochlorophyll aggregates is proportional to the length of the esterifying alcohol PHOTOSYNTH RES 104 211-219, 2010
DOI:10.1007/s11120-010-9541-0

Bohmova K, Hadravova R, Stokrova J, Tuma R, Ruml T, Pichova I, Rumlova M Effect of Dimerizing Domains and Basic Residues on In Vitro and In Vivo Assembly of Mason-Pfizer Monkey Virus and Human Immunodeficiency Virus J VIROL 84 1977-1988, 2010
DOI:10.1128/JVI.02022-09

Tuma R Chapter 9: Hexameric viral RNA helicases RSC Biomolecular Sciences 213-242, 2010

Tuma R, Jankowsky E Hexameric Viral RNA Helicases RNA HELICASES 213-242, 2010
DOI:10.1039/9781849732215-00213

Psencik J, Collins AM, Liljeroos L, Torkkeli M, Laurinmaki P, Ansink HM, Ikonen TP, Serimaa RE, Blankenship RE, Tuma R, Butcher SJ Structure of Chlorosomes from the Green Filamentous Bacterium Chloroflexus aurantiacus J BACTERIOL 191 6701-6708, 2009
DOI:10.1128/JB.00690-09

Tuma R, Tsuruta H, French KH, Prevelige PE Detection of intermediates and kinetic control during assembly of bacteriophage P22 procapsid J MOL BIOL 381 1395-1406, 2008
DOI:10.1016/j.jmb.2008.06.020

Wallin AE, Ojala H, Hæggström E, Tuma R Stiffer optical tweezers through real-time feedback control Applied Physics Letters 92 -, 2008
DOI:10.1063/1.2940339
View abstract

Ramani R, Hanski S, Laiho A, Tuma R, Kilpelainen S, Tuomisto F, Ruokolainen J, Ikkala O Evidence of PPII-like helical conformation and glass transition in a self-assembled solid-state polypeptide-surfactant complex: Poly(L-histidine)/docylbenzenesulfonic acid BIOMACROMOLECULES 9 1390-1397, 2008
DOI:10.1021/bm7012845

Suchanova B, Tuma R Folding and assembly of large macromolecular complexes monitored by hydrogen-deuterium exchange and mass spectrometry MICROB CELL FACT 7 -, 2008
DOI:10.1186/1475-2859-7-12

Arellano JB, Torkkeli M, Tuma R, Laurinmaki P, Melo TB, Ikonen TP, Butcher SJ, Serimaa RE, Psencik J Hexanol-induced order-disorder transitions in lamellar self-assembling aggregates of bacteriochlorophyll c in Chlorobium tepidum chlorosomes LANGMUIR 24 2035-2041, 2008
DOI:10.1021/la703024e

Kainov DE, Mancini EJ, Telenius J, Lisai J, Grimes JM, Bamford DH, Stuart DI, Tuma R Structural basis of mechanochemical coupling in a hexameric molecular motor J BIOL CHEM 283 3607-3617, 2008
DOI:10.1074/jbc.M706366200

Telenius J, Wallin AE, Straka M, Zhang HB, Mancini EJ, Tuma R RNA packaging motor: From structure to quantum mechanical modelling and sequential-stochastic mechanism COMPUT MATH METHOD M 9 351-369, 2008
DOI:10.1080/17486700802168502

Wallin AE, Ojala H, Korsbäck A, Hæggström E, Tuma R Real-time control of optical tweezers Proceedings of SPIE - The International Society for Optical Engineering 6644 -, 2007
DOI:10.1117/12.737269
View abstract

Astier Y, Kainov DE, Bayley H, Tuma R, Howorka S Stochastic detection of motor protein-RNA complexes by single-channel current recording CHEMPHYSCHEM 8 2189-2194, 2007
DOI:10.1002/cphc.200700179

Mancini EJ, Assenberg R, Verma A, Walter TS, Tuma R, Grimes JM, Owens RJ, Stuart DI Structure of the Murray Valley encephalitis virus RNA helicase at 1.9 angstrom resolution PROTEIN SCI 16 2294-2300, 2007
DOI:10.1110/ps.072843107

Wallin AE, Salmi A, Tumay R Step length measurement - Theory and simulation for tethered bead constant-force single molecule assay BIOPHYS J 93 795-805, 2007
DOI:10.1529/biophysj.106.097915

Suchanova B, Manole V, Kang S, Happonen L, Ora A, Prevelige P, Butcher S, Tuma R Protein dynamics and assembly of dsRNA bacteriophage by hydrogen/deuterium exchange detected by mass spectrometry MOL CELL PROTEOMICS 6 26-26, 2007

Ikonen TP, Li H, Psencik J, Laurinmaki PA, Butcher SJ, Frigaard NU, Serimaa RE, Bryant DA, Tuma R X-ray scattering and electron cryomicroscopy study on the effect of carotenoid biosynthesis to the structure of Chlorobium tepidum chlorosomes BIOPHYS J 93 620-628, 2007
DOI:10.1529/biophysj.106.101444

Zabranska H, Tuma R, Kuhl I, Svatos A, Ruml T, Hrabal R, Pichova I The role of the S-S bridge in retroviral protease function and virion maturation J MOL BIOL 365 1493-1504, 2007
DOI:10.1016/j.jmb.2006.11.005

Psencik J, Laurinmaki P, Butcher S, Serimaa R, Tuma R Changes in the lamellar organization of bacteriochlorophyll aggregates in chlorosomes upon hexanol treatment PHOTOSYNTH RES 91 170-171, 2007

Psencik J, Arellano JB, Ikonen TP, Borrego CM, Laurinmaki PA, Butcher SJ, Serimaa RE, Tuma R Internal structure of chlorosomes from brown-colored Chlorobium species and the role of carotenoids in their assembly BIOPHYS J 91 1433-1440, 2006
DOI:10.1529/biophysj.106.084228

Lisal J, Kainov DE, Lam TT, Emmett MR, Wei H, Gottlieb P, Marshall AG, Tuma R Interaction of packaging motor with the polymerase complex of dsRNA bacteriophage VIROLOGY 351 73-79, 2006
DOI:10.1016/j.virol.2006.03.025

Kainov DE, Tuma R, Mancini EJ Hexameric molecular motors: P4 packaging ATPase unravels the mechanism CELL MOL LIFE SCI 63 1095-1105, 2006
DOI:10.1007/s00018-005-5450-3

Lisal J, Tuma R Cooperative mechanism of RNA packaging motor Journal of Biological Chemistry 280 23157-23164, 2005
View abstract

Tuma R, Tsuruta H, Prevelige PE, Kainov DE, Poranen MM, Bamford DH In vitro assembly of bacteriophages: Folding, kinetic control and intermediates Journal of Theoretical Medicine 6 139-, 2005
DOI:10.1080/08893110500150073
View abstract

Lisal J, Lam TT, Kainov DE, Emmett MR, Marshall AG, Tuma R Functional visualization of viral molecular motor by hydrogen-deuterium exchange reveals transient states Nature Structural&Molecular Biology 12 460-466, 2005
DOI:10.1038/nsmb927
View abstract

Tuma R Raman spectroscopy of proteins: from peptides to large assemblies J RAMAN SPECTROSC 36 307-319, 2005
DOI:10.1002/jrs.1323

Merckel MC, Huiskonen JT, Goldman A, Bamford DM, Tuma R The structure of the bacteriophage PRD1 spike sheds light on the evolution of viral capsid architecture Molecular Cell 18 161-170, 2005
View abstract

Poranen MM, Tuma R, Bamford DH Assembly of double-stranded RNA bacteriophages. Adv Virus Res 64 15-43, 2005
DOI:10.1016/S0065-3527(05)64002-X

Kainov DE, Simonov V, Bamford DH, Tuma R, Gottlieb P, Wei H, Walsh MA, Belrhali H, Merckel MC Crystallization and preliminary X-ray diffraction analysis of bacteriophage varphi12 packaging factor P7. Acta Crystallogr D Biol Crystallogr 60 2368-2370, 2004
DOI:10.1107/S0907444904026952
View abstract

Mancini EJ, Kainov DE, Grimes JM, Tuma R, Bamford DH, Stuart DI Atomic snapshots of an RNA packaging motor reveal conformational changes linking ATP hydrolysis to RNA translocation Cell 118 743-755, 2004
DOI:10.1016/j.cell.2004.09.007
View abstract

Psencik J, Ikonen TP, Laurinmaki P, Merckel MC, Butcher SJ, Serimaa RE, Tuma R Lamellar organization of pigments in chlorosomes, the light harvesting complexes of green photosynthetic bacteria Biophysical Journal 87 1165-1172, 2004
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Poranen MM, Tuma R Self-assembly of double-stranded RNA bacteriophages Virus Research 101 93-100, 2004
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Mancini EJ, Kainov DE, Hui W, Gottlieb P, Tuma R, Bamford DH, Stuart DI, Grimes JM Production, crystallization and preliminary X-ray crystallographic studies of the bacteriophage phi 12 packaging motor Acta Crystallographica. Section D: Biological Crystallography 588-590, 2004
View abstract

Kainov DE, Lísal J, Bamford DH, Tuma R Packaging motor from double-stranded RNA bacteriophage phi12 acts as an obligatory passive conduit during transcription. Nucleic Acids Res 32 3515-3521, 2004
DOI:10.1093/nar/gkh680
View abstract

Lisal J, Kainov DE, Bamford DH, Thomas Jr GJ, Tuma R Enzymatic mechanism of RNA translocation in double-stranded RNA Bacteriophages Journal of Biological Chemistry 279 1343-1350, 2004
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Mancini EJ, Grimes JM, malby R, Sutton GC, Kainov DE, Juuti JT, Makeyev EV, Tuma R, Bamford DH, Stuart DI Order and disorder in crystals of hexameric NTPases from dsRNA bacteriophages Acta Crystallographica. Section D: Biological Crystallography 59 2337-2341, 2003
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Kainov DE, Pirttimaa M, Tuma R, Butcher SJ, Thomas Jr GJ, Bamford DH, Makeyev EV RNA packaging device of double-stranded RNA bacteriophages, possibly as simple as hexamer of P4 protein Journal of Biological Chemistry 278 48084-48091, 2003
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Ikonen T, Kainov D, Timmins P, Serimaa R, Tuma R Locating the minor components of double-stranded RNA bacteriophage phi 6 by neutron scattering JOURNAL OF APPLIED CRYSTALLOGRAPHY 36 525-529, 2003
DOI:10.1107/S0021889803001857

Kainov DE, Butcher SJ, Bamford DH, Tuma R Conserved intermediates on the assembly pathway of double-stranded RNA bacteriophages J MOL BIOL 328 791-804, 2003
DOI:10.1016/S0022-2836(03)00322-X

Ojala PJ, Paavilainen VO, Vartiainen MK, Tuma R, Weeds AG, Lappalalainen P The two ADF-H domains of twinfilin play functionally distinct roles in interactions with actin monomers Molecular Biology of the Cell 13 3811-3821, 2002
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Benevides JM, Juuti JT, Tuma R, Bamford DH, Thomas GJ Characterization of subunit-specific interactions in a double-stranded RNA virus: Raman difference spectroscopy of the phi 6 procapsid BIOCHEMISTRY-US 41 11946-11953, 2002
DOI:10.1021/bi0201623

Tuma R, Kainov D, Bamford DH In vitro assembly of dsRNA bacteriophage phi 8. BIOPHYS J 82 325A-325A, 2002

Sokolova A, Malfois M, Caldentey J, Svergun DI, Koch MH, Bamford DH, Tuma R Solution structure of bacteriophage PRD1 vertex complex Journal of Biological Chemistry 276 46187-46195, 2001
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Tuma R, Russell MP, Rosendahl M, Thomas Jr GJ SOLUTION CONFORMATION OF THE EXTRACELLULAR DOMAIN OF THE HUMAN TUMOR-NECROSIS-FACTOR RECEPTOR PROBED BY RAMAN AND UV-RESONANCE RAMAN-SPECTROSCOPY - STRUCTURAL EFFECTS OF AN ENGINEERED PEG LINKER Biochemistry 34 15150-15156, 2001
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Poranen MM, Paatero AO, Tuma R, Bamford DH Self-assembly of a viral molecular machine from purified protein and RNA constituents Molecular Cell 7 845-854, 2001
DOI:10.1016/S1097-2765(01)00228-3
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Tuma R, Coward LU, Kirk MC, Barnes S, Prevelige PE Hydrogen-deuterium exchange as a probe of folding and assembly in viral capsids Journal of Molecular Biology 306 389-396, 2001
DOI:10.1006/jmbi.2000.4383
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Tuma R, Tsuruta H, Benevides JM, Prevelige PE, Thomas GJ Characterization of subunit structural changes accompanying assembly of the bacteriophage P22 procapsid BIOCHEMISTRY-US 40 665-674, 2001
DOI:10.1021/bi001965y

Thomas GJ, Benevides JM, Tuma R, Juuti J, Bamford DH Raman spectroscopy of the dsRNA bacteriophage Phi 6 procapsid and precursors: Structural determinants in the assembly of an RNA polymerase core BIOPHYS J 80 293A-294A, 2001

Tuma R, Tsuruta H, Prevelige PE Assembly of bacteriophage P22 studied by solution X-ray scattering. BIOPHYS J 80 401A-401A, 2001

Caldentey J, Tuma R, Bamford DH Assembly of bacteriophage PRD1 spike complex: Role of the multidomain protein P5 BIOCHEMISTRY-US 39 10566-10573, 2000

Tuma R, Bamford JKH, Bamford DH, Thomas GJ Assembly dynamics of the nucleocapsid shell subunit (P8) of bacteriophage phi 6 BIOCHEMISTRY-US 38 15025-15033, 1999

Lanman J, Tuma R, Prevelige PE Identification and characterization of the domain structure of bacteriophage P22 coat protein BIOCHEMISTRY-US 38 14614-14623, 1999

de Sousa PC, Tuma R, Prevelige PE, Silva JL, Foguel D Cavity defects in the procapsid of bacteriophage P22 and the mechanism of capsid maturation J MOL BIOL 287 527-538, 1999

Jenkins RH, Tuma R, Juuti JT, Bamford DH, Thomas GJ Raman spectral studies of nucleic acids series. part LXIX - A novel Raman spectrophotometric method for quantitative measurement of nucleoside triphosphate hydrolysis BIOSPECTROSCOPY 5 3-8, 1999

Tuma R, Coward LU, Kirk MC, Barnes S, Prevelige PE Folding and assembly dynamics of bacteriophage P22 coat protein BIOPHYS J 76 A142-A142, 1999

Tuma R, Parker MH, Weigele P, Sampson L, Sun Y, Krishna NR, Casjens S, Thomas GJ, Prevelige PE A helical coat protein recognition domain of the bacteriophage P22 scaffolding protein. J Mol Biol 281 81-94, 1998
DOI:10.1006/jmbi.1998.1916
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Tuma R, Prevelige PE, Thomas Jr GJ Mechanism of capsid maturation in a double-stranded DNA virus Proceedings of the National Academy of Sciences of the United States of America 95 9885-9890, 1998
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Juuti JT, Bamford DH, Tuma R, Thomas Jr GJ Structure and NTPase activity of the RNA-translocating protein (P4) of bacteriophage phi 6 Journal of Molecular Biology 279 347-359, 1998
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Tuma R, Prevelige PE, Bamford DH, Thomas GJ Time-resolved Raman spectroscopy for investigation of hydrogen isotope exchanges in proteins, nucleic acids and nucleoprotein assemblies. BIOPHYS J 74 A29-A29, 1998

Tuma R, Parker MH, Prevelige PE, Thomas GJ Structure and function of the C-terminal domain of phage P22 scaffolding protein. BIOPHYS J 74 A72-A72, 1998

Vohnik S, Hanson C, Tuma R, Fuchs JA, Woodward C, Thomas GJ Conformation, stability, and active-site cysteine titrations of Escherichia coli D26A thioredoxin probed by Raman spectroscopy PROTEIN SCI 7 193-200, 1998

Tuma R, Thomas GJ Mechanisms of virus assembly probed by Raman spectroscopy: The icosahedral bacteriophage P22 BIOPHYSICAL CHEMISTRY 68 17-31, 1997
DOI:10.1016/S0301-4622(97)00001-X

Tuma R, Prevelige PE, Thomas GJ Folding intermediates in virus assembly: Raman dynamic probe of procapsid maturation in the dsDNA phage P22. BIOPHYS J 72 WAMB3-WAMB3, 1997

Tuma R, Thomas Jr GJ Theory, design, and characterization of a microdialysis flow cell for Raman spectroscopy Biophysical Journal 71 3454-3466, 1996
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Tuma R, Prevelige PE, Thomas Jr GJ Structural transitions in the scaffolding and coat proteins of P22 virus during assembly and disassembly Biochemistry 35 4619-4627, 1996
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Tuma R, Bamford JK, Bamford DH, Thomas Jr GJ Structure, interactions and dynamics of PRD1 virus .2. Organization of the viral membrane and DNA Journal of Molecular Biology 257 102-115, 1996
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Tuma R, Bamford JK, Bamford DH, Russell MP, Thomas Jr GJ Structure, interactions and dynamics of PRD1 virus .1. Coupling of subunit folding and capsid assembly Journal of Molecular Biology 257 87-101, 1996
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WALIN L, TUMA R, THOMAS GJ, BAMFORD DH PURIFICATION OF VIRUSES AND MACROMOLECULAR ASSEMBLIES FOR STRUCTURAL INVESTIGATIONS USING A NOVEL ION-EXCHANGE METHOD VIROLOGY 201 1-7, 1994

TUMA R, BAMFORD DH, THOMAS GJ STRUCTURE, INTERACTIONS AND THERMOSTABILITY OF THE MEMBRANE-PACKAGED DSDNA GENOME OF BACTERIOPHAGE PRD1 BIOPHYS J 66 A296-A296, 1994

Tuma R, Vohnik S, Li H, Thomas Jr GJ CYSTEINE CONFORMATION AND SULFHYDRYL INTERACTIONS IN PROTEINS AND VIRUSES .3. QUANTITATIVE MEASUREMENT OF THE RAMAN S-H BAND INTENSITY AND FREQUENCY Biophysical Journal 65 1066-1072, 1993
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Patel N, Wroblewski E, Leonov G, Phillips S, Tuma R, Twarock R, Stockley PG Rewriting Nature’s Assembly Manual for a ssRNA Virus Proceedings of the National Academy of Sciences of the United States of America -,
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