Faculty of Biological Sciences

Prof Nicola Stonehouse

BSc, UEA; PhD 1992, Leeds.
Professor of Molecular Virology
School of Molecular and Cellular Biology

Contact:  Garstang 8.53b | +44(0) 113 34 33102 | email address for  

You can read more about Prof Stonehouse's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=NJS

Research Interests

Viral replication and assembly

The Stonehouse group have interests in virology and RNA biology, applying novel approaches to the study of virus replication and assembly in three main research areas:

1) We have a long-standing interest in picornaviral replication, especially the role of the viral polymerase of foot-and-mouth disease virus. Current projects, funded by the Wellcome Trust and BBSRC include the use of RNA aptamers as novel molecular tools.
 
2) We also use aptamers for the study of the oncogenes of human papillomavirus (the causative agent of cervical and other cancers).
 
3) We use structure-based methods in the design of novel vaccines e.g. to polio and EV71. THis work is funded by WHO/Gates foundation and Sanofi Pasteur.
 

Faculty Research and Innovation


Studentship information

Postgraduate studentship areas:

  • Use of RNA aptamers to study viral proteins and inhibit their function
  • Viral assembly and vaccine development

See also:

Admin roles

Chair of Exams Group of Graduate board
FBS Graduate School Committee
Wellcome Trust Module Manager

Modules managed

MICR3120 - Adv Topics: Microbiology 1

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC3111/12/13/BIOL3112/MICR3120 - ATU 36 - Visualising viruses
BIOC3160 - Lab/Lit/Comp Research Project
BIOL1302 - Intro Skills - Biological Sci
BIOL2301 - Intermediate Skills for BioSci
BIOL3305 - Adv Skills in the Biosciences
BIOL3306 - Biol Sciences Research Project
BIOL3399 - Extended Research Project Preparation
BIOL5243M - Topics in Cancer Biology
BIOW5903X - Structure/Function of Nucleic Acids
BLGY3110 - Applied Genetics
BMSC2120 - Scientific Skills
MICR1120/3342 - Introduction to Microbiology
MICR2121 - Molecular Virology
MICR2222 - Medical Virology
MICR3120 - Adv Topics: Microbiology 1
MICR3120/BMSC3143/44/45/46 b - ATU 29 - Respiratory infections
MICR3190 - Microbiology Research Programme

Committees

Member of Graduate School Committee

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Prof Nicola Stonehouse  (Professor of Molecular Virology)

Viral replication and assembly 


Dr Morgan Herod  (Research Fellow)

Mrs Susan Matthews  (Research Technician)

Dr Clare Nicol  (Research Fellow)

Dr Sam Stephen  (Research Fellow)


Postgraduates

Oluwapelumi Adeyemi  
Ozlem Cesur  
Julian Crandall Hollick  
James Kelly  
Joseph Lattimer  
Eleni-Anna Loundras  
Grace Roberts  
Thana Sutthibutpong  
Jessica Swanson  
Joseph Ward  

Tulloch F; Pathania U; Luke GA; Nicholson J; Stonehouse NJ; Rowlands DJ; Jackson T; Tuthill T; Haas J; Lamond AI; Ryan MD FMDV replicons encoding green fluorescent protein are replication competent. Journal of virological methods -, 2014
DOI:10.1016/j.jviromet.2014.08.020
View abstract

Forrest S; Lear Z; Herod MR; Ryan M; Rowlands DJ; Stonehouse NJ Inhibition of the FMDV sub-genomic replicon by RNA aptamers. J Gen Virol -, 2014
DOI:10.1099/vir.0.067751-0
View abstract

Panjwani A; Strauss M; Gold S; Wenham H; Jackson T; Chou JJ; Rowlands DJ; Stonehouse NJ; Hogle JM; Tuthill TJ Capsid protein VP4 of human rhinovirus induces membrane permeability by the formation of a size-selective multimeric pore. PLoS Pathog 10 e1004294-, 2014
DOI:10.1371/journal.ppat.1004294
View abstract

Belyaeva TA; Nicol C; Cesur O; Travé G; Blair GE; Stonehouse NJ An RNA Aptamer Targets the PDZ-Binding Motif of the HPV16 E6 Oncoprotein. Cancers 6 1553-1569, 2014
DOI:10.3390/cancers6031553
View abstract

Shepherd DA; Ariza A; Edwards TA; Barr JN; Stonehouse NJ; Ashcroft AE Probing Bunyavirus N protein oligomerisation using mass spectrometry Rapid Communications in Mass Spectrometry 28 793-800, 2014
DOI:10.1002/rcm.6841
View abstract

Doble R; McDermott MF; Cesur O; Stonehouse NJ; Wittmann M Could RNA aptamers be utilized for the topical treatment of inflammatory skin diseases?, 2014

Doble R; McDermott MF; Cesur O; Stonehouse NJ; Wittmann M IL-17A RNA aptamer: possible therapeutic potential in some cells, more than we bargained for in others? Journal of Investigative Dermatology 134 852-855, 2014
DOI:10.1038/jid.2013.399

Doble R; McDermott MF; Cesur O; Stonehouse NJ; Wittmann M Are RNA aptamers suitable therapeutic tools for topical treatment of inflammatory skin diseases?, 2014

Shepherd DA; Holmes K; Rowlands DJ; Stonehouse NJ; Ashcroft AE Using ion mobility spectrometry-mass spectrometry to decipher the conformational and assembly characteristics of the hepatitis B capsid protein. Biophysical Journal 105 1258-1267, 2013
DOI:10.1016/j.bpj.2013.07.028
View abstract

Ariza A; Tanner SJ; Walter CT; Dent KC; Shepherd DA; Wu W; Matthews SV; Hiscox JA; Green TJ; Luo M; Elliott RM; Fooks AR; Ashcroft AE; Stonehouse NJ; Ranson NA; Barr JN; Edwards TA Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization. Nucleic Acids Research 41 5912-5926, 2013
DOI:10.1093/nar/gkt268
View abstract

Nicol C; CesurÖ; Forrest S; Belyaeva TA; Bunka DH; Blair GE; Stonehouse NJ An RNA aptamer provides a novel approach for the induction of apoptosis by targeting the HPV16 E7 oncoprotein. PLoS One 8 e64781-, 2013
DOI:10.1371/journal.pone.0064781
View abstract

Wetherill LF; Holmes KK; Verow M; Müller M; Howell G; Harris M; Fishwick C; Stonehouse N; Foster R; Blair GE; Griffin S; Macdonald A High-risk human papillomavirus E5 oncoprotein displays channel-forming activity sensitive to small-molecule inhibitors. J Virol 86 5341-5351, 2012
DOI:10.1128/JVI.06243-11
View abstract

Bentham M; Holmes K; Forrest S; Rowlands DJ; Stonehouse NJ Formation of higher-order foot-and-mouth disease virus 3D(pol) complexes is dependent on elongation activity. J Virol 86 2371-2374, 2012
DOI:10.1128/JVI.05696-11
View abstract

Foster TL; Gallay P; Stonehouse NJ; Harris M Cyclophilin A Interacts with Domain II of Hepatitis C Virus NS5A and Stimulates RNA Binding in an Isomerase-Dependent Manner J VIROL 85 7460-7464, 2011
DOI:10.1128/JVI.00393-11

Nicol C; Bunka DH; Blair GE; Stonehouse NJ Effects of single nucleotide changes on the binding and activity of RNA aptamers to human papillomavirus 16 E7 oncoprotein. Biochem Biophys Res Commun 405 417-421, 2011
DOI:10.1016/j.bbrc.2011.01.044
View abstract

Knapman TW; Morton VL; Stonehouse NJ; Stockley PG; Ashcroft AE Determining the topology of virus assembly intermediates using ion mobility spectrometry-mass spectrometry RAPID COMMUN MASS SP 24 3033-3042, 2010
DOI:10.1002/rcm.4732

Foster TL; Belyaeva T; Stonehouse NJ; Pearson AR; Harris M All Three Domains of the Hepatitis C Virus Nonstructural NS5A Protein Contribute to RNA Binding J VIROL 84 9267-9277, 2010
DOI:10.1128/JVI.00616-10

Morton VL; Dykeman EC; Stonehouse NJ; Ashcroft AE; Twarock R; Stockley PG The Impact of Viral RNA on Assembly Pathway Selection J MOL BIOL 401 298-308, 2010
DOI:10.1016/j.jmb.2010.05.059

Basnak G; Morton VL; Rolfsson O; Stonehouse NJ; Ashcroft AE; Stockley PG Viral Genomic Single-Stranded RNA Directs the Pathway Toward a T=3 Capsid J MOL BIOL 395 924-936, 2010
DOI:10.1016/j.jmb.2009.11.018

Morton VL; Burkitt W; O'Connor G; Stonehouse NJ; Stockley PG; Ashcroft AE RNA-induced conformational changes in a viral coat protein studied by hydrogen/deuterium exchange mass spectrometry PHYS CHEM CHEM PHYS 12 13468-13475, 2010
DOI:10.1039/c0cp00817f

Morton VL; Stockley PG; Stonehouse NJ; Ashcroft AE INSIGHTS INTO VIRUS CAPSID ASSEMBLY FROM NON-COVALENT MASS SPECTROMETRY MASS SPECTROM REV 27 575-595, 2008
DOI:10.1002/mas.20176

Rolfsson O; Toropova K; Morton V; Francese S; Basnak G; Thompson GS; Homans SW; Ashcroft AE; Stonehouse NJ; Ranson NA; Stockley PG RNA packing specificity and folding during assembly of the bacteriophage MS2 COMPUT MATH METHOD M 9 339-349, 2008
DOI:10.1080/17486700802168445

Tolley AC; Stonehouse NJ Conformational changes in the connector protein complex of the bacteriophage phi 29 DNA packaging motor COMPUT MATH METHOD M 9 327-337, 2008
DOI:10.1080/17486700802168486

Stockley PG; Rolfsson O; Thompson GS; Basnak G; Francese S; Stonehouse NJ; Homans SW; Ashcroft AE A simple, RNA-mediated allosteric switch controls the pathway to formation of a T=3 viral capsid J MOL BIOL 369 541-552, 2007
DOI:10.1016/j.jmb.2007.03.020

Ellingham M; Bunka DHJ; Rowlands DJ; Stonehouse NJ Selection and characterization of RNA aptamers to the RNA-dependent RNA polymerase from foot-and-mouth disease virus RNA 12 1970-1979, 2006
DOI:10.1261/rna.161006

Horn WT; Tars K; Grahn E; Heigstrand C; Baron AJ; Lago H; Adams CJ; Peabody DS; Phillips SEV; Stonehouse NJ; Liljas L; Stockley PG Structural basis of RNA binding discrimination between bacteriophages Q beta and MS2 STRUCTURE 14 487-495, 2006
DOI:10.1016/j.str.2005.12.006

Robinson MA; Wood JPA; Capaldi SA; Baron AJ; Gell C; Smith DA; Stonehouse NJ Affinity of molecular interactions in the bacteriophage phi 29 DNA packaging motor NUCLEIC ACIDS RES 34 2698-2709, 2006
DOI:10.1093/nar/gkl318

Ashcroft AE; Lago H; Macedo JMB; Horn WT; Stonehouse NJ; Stockley PG Engineering thermal stability in RNA phage capsids via disulphide bonds J NANOSCI NANOTECHNO 5 2034-2041, 2005
DOI:10.1166/jnn.2005.507

Wood JPA; Capaldi SA; Robinson MA; Baron AJ; Stonehouse NJ RNA multimerisation in the DNA packaging motor of bacteriophage phi29 Journal of Theoretical Medicine 6 127-134, 2005
DOI:10.1080/10273660500149802

Stockley PG; Ashcroft AE; Francese S; Thompson GS; Ranson NA; Smith AM; Homans SW; Stonehouse NJ Dissecting the fine details of assembly of a T=3 capsid Journal of Theoretical Medicine 6 119-125, 2005
DOI:10.1080/10273660500149869

Hall J; Moll D; Zhang W; Guo P; Stonehouse N; Urquidi-Macdonald M Determination of molecular size, distance and stochiometry of bionanoparticles using nanoscale measurement techniques In Handbook of nanostructured biomaterials and their applications in nanobiotechnology , 2005

Horn WT; Convery MA; Stonehouse NJ; Adams CJ; Liljas L; Phillips SEV; Stockley PG The crystal structure of a high affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: Further challenges in the modeling of ligand-RNA interactions RNA 10 1776-1782, 2004
DOI:10.1261/rna.7710304

Helgstrand C; Grahn E; Moss T; Stonehouse NJ; Tars K; Stockley PG; Liljas L Investigating the structural basis of purine specificity in the structures of MS2 coat protein RNA translational operator hairpins NUCLEIC ACIDS RES 30 2678-2685, 2002

Grahn E; Moss T; Helgstrand C; Fridborg K; Sundaram M; Tars K; Lago H; Stonehouse NJ; Davis DR; Stockley PG; Liljas L Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex RNA 7 1616-1627, 2001

Lago H; Parrott AM; Moss T; Stonehouse NJ; Stockley PG Probing the kinetics of formation of the bacteriophage MS2 translational operator complex: Identification of a protein conformer unable to bind RNA J MOL BIOL 305 1131-1144, 2001

Grahn E; Stonehouse NJ; Adams CJ; Fridborg K; Beigelman L; Matulic-Adamic J; Warriner SL; Stockley PG; Liljas L Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface Nucleic Acids Research 28 4611-4616, 2000
View abstract

Parrott A; Lago-Zarrilli HAH; Adams CJ; Ashcroft AE; Stonehouse NJ; Stockley PG RNA aptamers for the MS2 bacteriophage coat protein have similar solution conformations and dynamics to the wild-type RNA translational operator Nucleic Acids Research 28 489-497, 2000
View abstract

Stonehouse NJ; Fonseca SA; Wood JPA RNA-protein interactions in the assembly of bacteriophage phi 29 (Abstract), 2000

Grahn E; Stonehouse NJ; Murray JB; van den Worm SHE; Valegard K; Fridborg K; Stockley PG; Lijas L Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements. RNA 5 131-138, 1999
View abstract

Stockley PG; Stonehouse NJ Virus Assembly and Morphogenesis In Development-genetics, epigentics and environmental regulation , 1999

Stonehouse NJ Dissecting the key recognition features of the MS2 bacteriophage translational repression complex Nucleic Acids Research 26 1337-1344, 1998

van den Worm SHE; Stonehouse NJ; Valegard K; Murray JB; Walton C; Fridborg K; Stockley PG Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments Nucleic Acids Research 26 1345-1351, 1998

Stonehouse NJ; Phillips SEV; Stockley PG; Convery MA; Rowsell S; Murray JB; Hirao I; Ellington AD; Peabody DS Crystal structure of an RNA aptamer protein complex at 2.8 angstrom resolution Nature Structural Biology 5 133-139, 1998
View abstract

Stonehouse NJ The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions Journal of Molecular Biology 270 724-738, 1997

Stonehouse NJ; Valegard K; Golmohammadi R; van den Worm SHE; Walton C; Stockley PG; Liljas L Crystal-structures of MS2 capsids with mutations in the subunit FG loop Journal of Molecular Biology 256 330-339, 1996

Stonehouse NJ Molecular-interactions in the RNA bacteriophage MS2 Biochemical Society Transactions 24 412-412, 1996

Stockley PG; Stonehouse NJ; Walton C Molecular mechanism of RNA Phage Morphogenesis Biochemical Society Transactions 21 627-633, 1993

Robinson C; Kirkham J; Brookes SJ; Stonehouse NJ Growth of Fluorosed Rat Enamel Crystals in vitro Journal of Dental Research 71 696-696, 1992

Shore RC; Robinson C; Kirkham J; Stonehouse NJ Development of Crystal Morphology in Rat Incisor Enamel Journal of Dental Research 71 728-728, 1992

Robinson C; Kirkham J; Shore RC; Stonehouse NJ Extracellular matrix enzymes and the control of enamel crystal growth Journal of Dental Research 70 350-350, 1991

Robinson C; Kirkham J; Shore RC; Stonehouse NJ Degradative activity in the developing tooth Journal of Dental Research 70 683-683, 1991

Robinson C; Shore RC; Kirkham J; Stonehouse NJ Extracellular processing of enamel matrix proteins and the control of crystal growth Journal de Biologie Buccale 18 355-361, 1990

Robinson C; Stonehouse NJ; Shore RC; Kirkham J Porous enamel hypoplasia: A new hypopthesis - the role of serum proteins Journal of Dental Research 69 967-967, 1990