Faculty of Biological Sciences

Prof Peter Stockley

BSc, London; PhD 1980, Cambridge.
Professor of Biological Chemistry
School of Molecular and Cellular Biology

Background: Studied Chemistry at Imperial College, London, before completing his PhD on Chromatin Structure in Prof. Jean Thomas' laboratory in Cambridge. Subsequently worked with Prof. Steve Harrison at Harvard on the mechanisms of virus assembly before joining the staff in Leeds in 1986.

Contact:  Miall 10.29 | +44(0) 113 34 33092 | email address for Prof Peter Stockley 

You can read more about Prof Stockley's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=PGS

please wait

Modules managed

BIOC1301 - Intro Inte Biochem Mol Process

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC1303 - Intro Biochem Problem Solving
BIOC2301 - Interm Integrated Biochemistry
BIOC2302 - Intermediate Biochem: Prac
BIOC2303 - Intermediate Biochem: Skills
BIOC3160 - Lab/Lit/Comp Research Project
BIOC3221/22/23/BIOL3210/MICR3211 b - ATU 30 - Exploiting the RNA world
BIOW5901X - Foundation module
BIOW5903X - Structure/Function of Nucleic Acids
BIOW5904X - Folding and Assembly

Centre membership:

Group Leader Prof Peter Stockley  (Professor of Biological Chemistry)

Protein-nucleic acid interactions, genetic regulatory mechanisms, virus assembly 


Dr Oleksandr Borodavka  (Sir Henry Wellcome Post Doctoral Fellow)

Dr David Bunka  (Visiting Research Fellow)

Mrs Amy Barker  (Research Technician)

Dr Nikesh Patel  (Research Fellow)

Dr Amit Sharma  (Research Fellow)


Postgraduates

Amy Barker  
Christopher Kirkham  
Rachel Mahood  
Atul Mohan  
Christopher Rowley  
Ruiyang Yan  

Sharma A; Leach RN; Gell C; Zhang N; Burrows PC; Shepherd DA; Wigneshweraraj S; Smith DA; Zhang X; Buck M; Stockley PG; Tuma R Domain movements of the enhancer-dependent sigma factor drive DNA delivery into the RNA polymerase active site: insights from single molecule studies. Nucleic Acids Res -, 2014
DOI:10.1093/nar/gku146
View abstract

Bakker SE; Groppelli E; Pearson AR; Stockley PG; Rowlands DJ; Ranson NA The limits of structural plasticity in a picornavirus capsid revealed by a massively expanded equine rhinitis A virus particle. J Virol -, 2014
DOI:10.1128/JVI.01979-13
View abstract

Galaway FA; Stockley PG MS2 viruslike particles: a robust, semisynthetic targeted drug delivery platform. Mol Pharm 10 59-68, 2013
DOI:10.1021/mp3003368
View abstract

Wnęk M; Górzny ML; Ward MB; Wälti C; Davies AG; Brydson R; Evans SD; Stockley PG Fabrication and characterization of gold nano-wires templated on virus-like arrays of tobacco mosaic virus coat proteins. Nanotechnology 24 025605-, 2013
DOI:10.1088/0957-4484/24/2/025605
View abstract

Sarell CJ; Woods LA; Su Y; Debelouchina GT; Ashcroft AE; Griffin RG; Stockley PG; Radford SE Expanding the repertoire of amyloid polymorphs by co-polymerization of related protein precursors. J Biol Chem 288 7327-7337, 2013
DOI:10.1074/jbc.M112.447524
View abstract

Borodavka A; Tuma R; Stockley PG A two-stage mechanism of viral RNA compaction revealed by single molecule fluorescence. RNA Biol 10 481-489, 2013
DOI:10.4161/rna.23838
View abstract

Keef T; Wardman JP; Twarock R; Ranson NA; Stockley PG Structural constraints on the three-dimensional geometry of simple viruses: Case studies of a new predictive tool Acta Crystallographica Section A: Foundations of Crystallography 69 140-150, 2013
DOI:10.1107/S0108767312047150
View abstract

Dykeman EC; Stockley PG; Twarock R Building a viral capsid in the presence of genomic RNA PHYSICAL REVIEW E 87 -, 2013
DOI:10.1103/PhysRevE.87.022717

Ford RJ; Barker AM; Bakker SE; Coutts RH; Ranson NA; Phillips SE; Pearson AR; Stockley PG Sequence-specific, RNA-protein interactions overcome electrostatic barriers preventing assembly of satellite tobacco necrosis virus coat protein. J Mol Biol 425 1050-1064, 2013
DOI:10.1016/j.jmb.2013.01.004
View abstract

Stockley PG; Barker AM; Borodavka A; Ford RJ; Pearson AR; Ranson NA; Tuma R; Twarock R; Dykeman E; Phillips SEV; Bakker SE Packaging signals in single-stranded RNA viruses: nature's alternative to a purely electrostatic assembly mechanism Journal of Biological Physics 1-11, 2013
DOI:10.1007/s10867-013-9313-0
View abstract

Stockley PG; Ranson NA; Twarock R A new paradigm for the roles of the genome in ssRNA viruses Future Virology 8 531-543, 2013
DOI:10.2217/fvl.12.84
View abstract

Dent K; Thompson R; Barker A; Hiscox J; Barr J; Stockley P; Ranson N The Asymmetric Structure of an Icosahedral Virus Bound to Its Receptor Suggests a Mechanism for Genome Release Structure 21 1225-1234, 2013
DOI:10.1016/j.str.2013.05.012
View abstract

Dykeman EC; Stockley PG; Twarock R Packaging Signals in Two Single-Stranded RNA Viruses Imply a Conserved Assembly Mechanism and Geometry of the Packaged Genome JOURNAL OF MOLECULAR BIOLOGY 425 3235-3249, 2013
DOI:10.1016/j.jmb.2013.06.005

Sarell CJ; Stockley PG; Radford SE Assessing the causes and consequences of co-polymerization in amyloid formation PRION 7 359-368, 2013
DOI:10.4161/pri.26415

Svobodova M; Nadal P; O'Sullivan CK; Bunka DHJ; Stockley PG Selection of 2′F-modified RNA aptamers against prostate-specific antigen and their evaluation for diagnostic and therapeutic applications Analytical and Bioanalytical Chemistry 1-9, 2013
DOI:10.1007/s00216-013-7350-y
View abstract

Svobodova M; Nadal P; O'Sullivan CK; Bunka DHJ; Stockley PG Selection of 2′F-modified RNA aptamers against prostate-specific antigen and their evaluation for diagnostic and therapeutic applications Analytical and Bioanalytical Chemistry 405 9149-9157, 2013
DOI:10.1007/s00216-013-7350-y
View abstract

Joce C; White R; Stockley PG; Warriner S; Turnbull WB; Nelson A Design, synthesis and in vitro evaluation of novel bivalent S-adenosylmethionine analogues. Bioorg Med Chem Lett 22 278-284, 2012
DOI:10.1016/j.bmcl.2011.11.017
View abstract

Derbyshire N; White SJ; Bunka DH; Song L; Stead S; Tarbin J; Sharman M; Zhou D; Stockley PG Toggled RNA aptamers against aminoglycosides allowing facile detection of antibiotics using gold nanoparticle assays. Anal Chem 84 6595-6602, 2012
DOI:10.1021/ac300815c
View abstract

Bakker SE; Ford RJ; Barker AM; Robottom J; Saunders K; Pearson AR; Ranson NA; Stockley PG Isolation of an asymmetric RNA uncoating intermediate for a single-stranded RNA plant virus. J Mol Biol 417 65-78, 2012
DOI:10.1016/j.jmb.2012.01.017
View abstract

White SJ; Morton DW; Cheah BC; Bronowska A; Davies AG; Stockley PG; Wälti C; Johnson S On-surface assembly of coiled-coil heterodimers. Langmuir 28 13877-13882, 2012
DOI:10.1021/la3025149
View abstract

Borodavka A; Tuma R; Stockley PG Evidence that viral RNAs have evolved for efficient, two-stage packaging Proceedings of the National Academy of Sciences of the United States of America 109 15769-15774, 2012
DOI:10.1073/pnas.1204357109
View abstract

White SJ; Johnson S; Szymonik M; Wardingley RA; Pye D; Davies AG; Wälti C; Stockley PG Directed surface attachment of nanomaterials via coiled-coil-driven self-assembly. Nanotechnology 23 495304-, 2012
DOI:10.1088/0957-4484/23/49/495304
View abstract

Bunka DHJ; Stockley PG Therapeutic applications of nucleic acid aptamer conjugates RSC Biomolecular Sciences 140-165, 2012

Zhang HY; Stockley PG; Zhou DJ Development of smart nanoparticle-aptamer sensing technology FARADAY DISCUSS 149 319-332, 2011
DOI:10.1039/c005373b

Dykeman EC; Grayson NE; Toropova K; Ranson NA; Stockley PG; Twarock R Simple Rules for Efficient Assembly Predict the Layout of a Packaged Viral RNA J MOL BIOL 408 399-407, 2011
DOI:10.1016/j.jmb.2011.02.039

Toropova K; Stockley PG; Ranson NA Visualising a Viral RNA Genome Poised for Release from Its Receptor Complex J MOL BIOL 408 408-419, 2011
DOI:10.1016/j.jmb.2011.02.040

Moore MD; Bunka DH; Forzan M; Spear PG; Stockley PG; McGowan I; James W Generation of neutralizing aptamers against herpes simplex virus type 2: potential components of multivalent microbicides. J Gen Virol 92 1493-1499, 2011
DOI:10.1099/vir.0.030601-0
View abstract

Moore MD; Bunka DHJ; Forzan M; Spear PG; Stockley PG; McGowan I; James W Generation of neutralizing aptamers against herpes simplex virus type 2: potential components of multivalent microbicides J GEN VIROL 92 1493-1499, 2011
DOI:10.1099/vir.0.030601-0

Lane SW; Dennis CA; Lane CL; Trinh CH; Rizkallah PJ; Stockley PG; Phillips SE Construction and crystal structure of recombinant STNV capsids. J Mol Biol 413 41-50, 2011
DOI:10.1016/j.jmb.2011.07.062
View abstract

Bunka DH; Lane SW; Lane CL; Dykeman EC; Ford RJ; Barker AM; Twarock R; Phillips SE; Stockley PG Degenerate RNA packaging signals in the genome of Satellite Tobacco Necrosis Virus: implications for the assembly of a T=1 capsid. J Mol Biol 413 51-65, 2011
DOI:10.1016/j.jmb.2011.07.063
View abstract

Lane SW; Dennis CA; Lane CL; Trinh CH; Stockley PG; Phillips SEV; Rizkallah PJ Construction and crystal structure of recombinant STNV capsids Journal of Molecular Biology 413 41-50, 2011
DOI:10.1016/j.jmb.2011.07.062
View abstract

Dykeman EC; Stockley PG; Twarock R Dynamic Allostery Controls Coat Protein Conformer Switching during MS2 Phage Assembly J MOL BIOL 395 916-923, 2010
DOI:10.1016/j.jmb.2009.11.016

Basnak G; Morton VL; Rolfsson O; Stonehouse NJ; Ashcroft AE; Stockley PG Viral Genomic Single-Stranded RNA Directs the Pathway Toward a T=3 Capsid J MOL BIOL 395 924-936, 2010
DOI:10.1016/j.jmb.2009.11.018

Bunka DHJ; Platonova O; Stockley PG Development of aptamer therapeutics CURR OPIN PHARMACOL 10 557-562, 2010
DOI:10.1016/j.coph.2010.06.009

Rolfsson O; Toropova K; Ranson NA; Stockley PG Mutually-induced conformational switching of RNA and coat protein underpins efficient assembly of a viral capsid. J Mol Biol 401 309-322, 2010
DOI:10.1016/j.jmb.2010.05.058
View abstract

Rolfsson O; Toropova K; Ranson NA; Stockley PG Mutually-induced Conformational Switching of RNA and Coat Protein Underpins Efficient Assembly of a Viral Capsid J MOL BIOL 401 309-322, 2010
DOI:10.1016/j.jmb.2010.05.058

Knapman TW; Morton VL; Stonehouse NJ; Stockley PG; Ashcroft AE Determining the topology of virus assembly intermediates using ion mobility spectrometry-mass spectrometry RAPID COMMUN MASS SP 24 3033-3042, 2010
DOI:10.1002/rcm.4732

Morton VL; Burkitt W; O'Connor G; Stonehouse NJ; Stockley PG; Ashcroft AE RNA-induced conformational changes in a viral coat protein studied by hydrogen/deuterium exchange mass spectrometry PHYS CHEM CHEM PHYS 12 13468-13475, 2010
DOI:10.1039/c0cp00817f

Stockley PG; Twarock R The physics of virus assembly PREFACE PHYS BIOL 7 -, 2010
DOI:10.1088/1478-3975/7/4/040301

Bunka DHJ; Platonova O; Stockley PG Development of aptamer therapeutics (vol 10, pg 557, 2010) CURR OPIN PHARMACOL 10 789-789, 2010
DOI:10.1016/j.coph.2010.09.010

Morton VL; Dykeman EC; Stonehouse NJ; Ashcroft AE; Twarock R; Stockley PG The Impact of Viral RNA on Assembly Pathway Selection J MOL BIOL 401 298-308, 2010
DOI:10.1016/j.jmb.2010.05.059

Morton V; Dykeman E; Stonehouse N; Ashcroft A; Twarock R; Stockley P The impact of viral RNA on assembly pathway selection J. Mol. Biol. in press-, 2010
DOI:10.1016/j.jmb.2010.05.059

Bunka DHJ; Platonova O; Stockley PG Erratum to "Development of aptamer therapeutics" [Curr. Opin. Pharmacol. 10 (2010) 557-562] Current Opinion in Pharmacology 10 789-789, 2010
DOI:10.1016/j.coph.2010.09.010

Stockley PG; Twarock R The physics of virus assembly Physical Biology 7 -, 2010
DOI:10.1088/1478-3975/7/4/040301

Manfield IW; Stockley PG Ethylation interference footprinting of DNA-protein complexes. Methods Mol Biol 543 105-120, 2009
DOI:10.1007/978-1-60327-015-1_9
View abstract

Barton JL; Bunka DHJ; Knowling SE; Lefevre P; Warren AJ; Bonifer C; Stockley PG Characterization of RNA aptamers that disrupt the RUNX1-CBF beta/DNA complex NUCLEIC ACIDS RES 37 6818-6830, 2009
DOI:10.1093/nar/gkp728

Ng CL; Waterman DG; Koonin EV; Walters AD; Chong JPJ; Isupov MN; Lebedev AA; Bunka DHJ; Stockley PG; Ortiz-Lombardia M; Antson AA Conformational flexibility and molecular interactions of an archaeal homologue of the Shwachman-Bodian-Diamond syndrome protein BMC STRUCT BIOL 9 -, 2009
DOI:10.1186/1472-6807-9-32

Camm KD; El-Sokkary A; Gott AL; Stockley PG; Belyaeva T; McGowan PC Synthesis, molecular structure and evaluation of new organometallic ruthenium anticancer agents DALTON T 10914-10925, 2009
DOI:10.1039/b918902e

Stockley PG; Persson B Surface plasmon resonance assays of DNA-protein interactions. Methods Mol Biol 543 653-669, 2009
DOI:10.1007/978-1-60327-015-1_38
View abstract

Stockley PG Filter-binding assays. Methods Mol Biol 543 1-14, 2009
DOI:10.1007/978-1-60327-015-1_1
View abstract

Joce C; Caryl J; Stockley PG; Warriner S; Nelson A Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNA ORG BIOMOL CHEM 7 635-638, 2009
DOI:10.1039/b816495a

Rolfsson O; Toropova K; Morton V; Francese S; Basnak G; Thompson GS; Homans SW; Ashcroft AE; Stonehouse NJ; Ranson NA; Stockley PG RNA packing specificity and folding during assembly of the bacteriophage MS2 COMPUT MATH METHOD M 9 339-349, 2008
DOI:10.1080/17486700802168445

Burrows PC; Wigneshweraraj S; Bose D; Joly N; Schumacher J; Rappas M; Pape T; Stockley PG; Zhang XD; Buck M Visualizing the organization and reorganization of transcription complexes for gene expression BIOCHEM SOC T 36 776-779, 2008
DOI:10.1042/BST0360776

Morton VL; Stockley PG; Stonehouse NJ; Ashcroft AE INSIGHTS INTO VIRUS CAPSID ASSEMBLY FROM NON-COVALENT MASS SPECTROMETRY MASS SPECTROM REV 27 575-595, 2008
DOI:10.1002/mas.20176

Gorzny ML; Walton AS; Wnek M; Stockley PG; Evans SD Four-probe electrical characterization of Pt-coated TMV-based nanostructures NANOTECHNOLOGY 19 -, 2008
DOI:10.1088/0957-4484/19/16/165704

Garnett JA; Marincs F; Baumberg S; Stockley PG; Phillips SEV Structure and function of the arginine repressor-operator complex from Bacillus subtilis J MOL BIOL 379 284-298, 2008
DOI:10.1016/j.jmb.2008.03.007

Toropova K; Basnak G; Twarock R; Stockley PG; Ranson NA The three-dimensional structure of genomic RNA in bacteriophage MS2: Implications for assembly J MOL BIOL 375 824-836, 2008
DOI:10.1016/j.jmb.2007.08.067

Gell C; Sabir T; Westwood J; Rashid A; Smith DAM; Harris SA; Stockley PG Single-Molecule Fluorescence Resonance Energy Transfer Assays Reveal Heterogeneous Folding Ensembles in a Simple RNA Stem-Loop J MOL BIOL 384 264-278, 2008
DOI:10.1016/j.jmb.2008.08.088

Madine J; Jack E; Stockley PG; Radford SE; Serpell LC; Middleton DA Structural Insights into the Polymorphism of Amyloid-Like Fibrils Formed by Region 20-29 of Amylin Revealed by Solid-State NMR and X-ray Fiber Diffraction J AM CHEM SOC 130 14990-15001, 2008
DOI:10.1021/ja802483d

Roach PCJ; O'Reilly J; Norbertczak HT; Hope RJ; Patching SG; Stockley PG; Baldwin SA; Rutherford NG; Henderson PJF; Venter H; Jamshad M; Bill RM; Herbert RB Equipping a Research Scale Fermentation Laboratory for Production of Membrane Proteins Practical Fermentation Technology 37-67, 2008
DOI:10.1002/9780470725306.ch3

Bunka DH; Mantle BJ; Morten IJ; Tennent GA; Radford SE; Stockley PG Production and characterization of RNA aptamers specific for amyloid fibril epitopes., 2007
DOI:10.1074/jbc.M703679200
View abstract

Bunka DHJ; Mantle BJ; Morten IJ; Tennent GA; Radford SE; Stockley PG Production and characterization of RNA aptamers specific for amyloid fibril epitopes J BIOL CHEM 282 34500-34509, 2007
DOI:10.1074/jbc.M703679200

Stockley PG; Rolfsson O; Thompson GS; Basnak G; Francese S; Stonehouse NJ; Homans SW; Ashcroft AE A simple, RNA-mediated allosteric switch controls the pathway to formation of a T=3 viral capsid J MOL BIOL 369 541-552, 2007
DOI:10.1016/j.jmb.2007.03.020

Garnett JA; Baumberg S; Stockley PG; Phillips SEV Structure of the C-terminal effector-binding domain of AhrC bound to its corepressor L-arginine ACTA CRYSTALLOGR F 63 918-921, 2007
DOI:10.1107/S1744309107049391

Toropova K; Basnak G; Twarock R; Stockley PG; Ranson NA The three-dimensional structure of genomic RNA in bacteriophage MS2: Implications for assembly, 2007
DOI:10.1016/j.jmb.2007.08.067

Garnett JA; Baumberg S; Stockley PG; Phillips SEV A high-resolution structure of the DNA-binding domain of AhrC, the arginine repressor/activator protein from Bacillus subtilis ACTA CRYSTALLOGR F 63 914-917, 2007
DOI:10.1107/S1744309107048166

Ironmonger A; Whittaker B; Baron AJ; Clique B; Adams CJ; Ashcroft AE; Stockley PG; Nelson A Scanning conformational space with a library of stereo- and regiochemically diverse aminoglycoside derivatives: the discovery of new ligands for RNA hairpin sequences ORG BIOMOL CHEM 5 1081-1086, 2007
DOI:10.1039/b618683a

Nevskaya N; Tishchenko S; Volchkov S; Kljashtorny V; Nikonova E; Nikonov O; Nikulin A; Kohrer C; Piendl W; Zimmermann R; Stockley PG; Garber M; Nikonov S New insights into the interaction of ribosornal protein L1 with RNA Journal of Molecular Biology 355 747-759, 2006
DOI:10.1016/j.jmb.2005.10.084
View abstract

Horn WT; Tars K; Grahn E; Heigstrand C; Baron AJ; Lago H; Adams CJ; Peabody DS; Phillips SEV; Stonehouse NJ; Liljas L; Stockley PG Structural basis of RNA binding discrimination between bacteriophages Q beta and MS2 STRUCTURE 14 487-495, 2006
DOI:10.1016/j.str.2005.12.006

Marincs F; Manfield IW; Stead JA; McDowall KJ; Stockley PG Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor. Biochem J 396 227-234, 2006
DOI:10.1042/BJ20060021
View abstract

Bunka DHJ; Stockley PG Aptamers come of age - at last NAT REV MICROBIOL 4 588-596, 2006
DOI:10.1038/nrmicro1458

Jack E; Newsome M; Stockley PG; Radford SE; Middleton DA The organization of aromatic side groups in an amyloid fibril probed by solid-state H-2 and F-19 NMR spectroscopy J AM CHEM SOC 128 8098-8099, 2006
DOI:10.1021/ja0581898

Leach RN; Gell C; Wigneshweraraj S; Buck M; Smith A; Stockley PG Mapping ATP-dependent activation at a sigma54 promoter. J Biol Chem 281 33717-33726, 2006
DOI:10.1074/jbc.M605731200
View abstract

Ironmonger A; Stockley PG; Nelson AS Asymmetric double ring-opening of a C-2h-symmetric bis-epoxide: improved enantiomeric excess of the product through enantioselective desymmetrisation and 'proof-reading' steps Organic&Biomolecular Chemistry 3 2350-2353, 2005
DOI:10.1039/b504972e
View abstract

Wu M; Sherwin T; Brown WL; Stockley PG Delivery of antisense oligonucleotides to leukemia cells by RNA bacteriophage capsids Nanomedicine 1 67-76, 2005

Clique B; Ironmonger A; Whittaker BP; Colley J; Titchmarsh J; Stockley PG; Nelson AS Synthesis of a library of stereo- and regiochemically diverse aminoglycoside derivatives Organic&Biomolecular Chemistry 3 2776-2785, 2005
DOI:10.1039/b505865a
View abstract

Stockley PG; Ashcroft AE; Francese S; Thompson GS; Ranson NA; Smith AM; Homans SW; Stonehouse NJ Dissecting the fine details of assembly of a T=3 capsid Journal of Theoretical Medicine 6 119-125, 2005
DOI:10.1080/10273660500149869

Ashcroft AE; Lago H; Macedo JMB; Horn WT; Stonehouse NJ; Stockley PG Engineering thermal stability in RNA phage capsids via disulphide bonds J NANOSCI NANOTECHNO 5 2034-2041, 2005
DOI:10.1166/jnn.2005.507

Marincs F; McDowall KJ; Stockley PG A combined in vitro transposition-in vivo recombination mutagenesis method to knock out genes in Escherichia coli American Biotechnology Laboratory 22 8-10, 2004

Stockley PG; Gell C; Sabir T; Leach R; Adams CJ; Wigneshveraraj S; Buck M; Smith AM Use Of Single-Molecule FRET And Fluorescence Correlation Spectroscopy To Probe RNA Folding And Transcriptional Initiation In Proceedings of International Symposium on Nanoscale Devices, Materials, and Biological Systems , 2004

Ryan DE; Kim CH; Murray JB; Adams CJ; Stockley PG; Abelson J New tertiary constraints between the RNA components of active yeast spliceosomes: A photo-crosslinking study RNA 10 1251-1265, 2004
DOI:10.1261/rna.7060404
View abstract

Stockley PG; Phillips SEV Repressor-operator recognition In Encyclopedia of Molecular Cell Biology and Molecular Medicine , 2004

Lawrenson ID; Stockley PG Kinetic analysis of operator binding by the E. coli methionine repressor highlights the role(s) of electrostatic interactions. FEBS Lett 564 136-142, 2004
DOI:10.1016/S0014-5793(04)00336-9
View abstract

Horn WT; Convery MA; Stonehouse NJ; Adams CJ; Liljas L; Phillips SE; Stockley PG The crystal structure of a high affinity RNA stem-loop complexed with the bacteriophage MS2 capsid: further challenges in the modeling of ligand-RNA interactions. RNA 10 1776-1782, 2004
DOI:10.1261/rna.7710304
View abstract

Stockley PG; Townsend R Whither biochemistry and chemistry The Biochemist 26 72-74, 2004

Yoburn JC; Deb S; Manfield IW; Stockley PG; Van Vranken DL A biaryl peptide crosslink in a MetJ peptide model confers cooperative, nonspecific binding to DNA that ablates both repressor binding and in vitro transcription. Bioorg Med Chem 11 811-816, 2003
View abstract

Wu M; Stockley PG; Martin WJ An improved Western blotting technique effectively reduces background ELECTROPHORESIS 23 2373-2376, 2002

He YY; Garvie CW; Elworthy S; Manfield IW; McNally T; Lawrenson ID; Phillips SE; Stockley PG Structural and functional studies of an intermediate on the pathway to operator binding by Escherichia coli MetJ. J Mol Biol 320 39-53, 2002
DOI:10.1016/S0022-2836(02)00423-0
View abstract

Brown WL; Mastico RA; Wu M; Heal KG; Adams CJ; Murray JB; Simpson JC; Lord JM; Taylor-Robinson AW; Stockley PG RNA bacteriophage capsid-mediated drug delivery and epitope presentation. Intervirology 45 371-380, 2002
View abstract

Helgstrand C; Grahn E; Moss T; Stonehouse NJ; Tars K; Stockley PG; Liljas L Investigating the structural basis of purine specificity in the structures of MS2 coat protein RNA translational operator hairpins. Nucleic Acids Res 30 2678-2685, 2002
View abstract

Stockley PG Filter-binding assays. Methods Mol Biol 148 1-11, 2001
DOI:10.1385/1-59259-208-2:001

Lago H; Parrott AM; Moss T; Stonehouse NJ; Stockley PG Probing the kinetics of formation of the bacteriophage MS2 translational operator complex: Identification of a protein conformer unable to bind RNA J MOL BIOL 305 1131-1144, 2001

Grahn E; Moss T; Helgstrand C; Fridborg K; Sundaram M; Tars K; Lago H; Stonehouse NJ; Davis DR; Stockley PG; Liljas L Structural basis of pyrimidine specificity in the MS2 RNA hairpin-coat-protein complex RNA 7 1616-1627, 2001

Manfield IW; Stockley PG Ethylation interference. Methods Mol Biol 148 229-243, 2001
DOI:10.1385/1-59259-208-2:229

Grahn E; Stonehouse NJ; Adams CJ; Fridborg K; Beigelman L; Matulic-Adamic J; Warriner SL; Stockley PG; Liljas L Deletion of a single hydrogen bonding atom from the MS2 RNA operator leads to dramatic rearrangements at the RNA-coat protein interface Nucleic Acids Research 28 4611-4616, 2000
View abstract

Heal KG; Hill HR; Stockley PG; Hollingdale MR; Taylor-Robinson AW Expression and immunogenicity of a liver stage malaria epitope presented as a foreign peptide on the surface of RNA-free MS2 bacteriophage capsids Vaccine 18 251-258, 2000

Argent RH; Parrott A; Day PJ; Roberts LM; Stockley PG; Lord JM; Radford SE Ribosome-mediated refolding of ricin A chain. Journal of Biological Chemistry 275 9263-9268, 2000
View abstract

Parrott A; Lago-Zarrilli HAH; Adams CJ; Ashcroft AE; Stonehouse NJ; Stockley PG RNA aptamers for the MS2 bacteriophage coat protein have similar solution conformations and dynamics to the wild-type RNA translational operator Nucleic Acids Research 28 489-497, 2000
View abstract

Stockley PG; Mastico RA Use of fusions to viral coat proteins as antigenic carriers for vaccine development. Methods Enzymol 326 551-569, 2000

Grahn E; Stonehouse NJ; Murray JB; van den Worm SHE; Valegard K; Fridborg K; Stockley PG; Lijas L Crystallographic studies of RNA hairpins in complexes with recombinant MS2 capsids: Implications for binding requirements. RNA 5 131-138, 1999
View abstract

Holtham C; Jumel K; Harding S; Baumberg S; Stockley PG Probing activation of the prokaryotic arginine transcriptional regulator using chimeric proteins Journal of Molecular Biology 289 707-727, 1999

Stockley PG; Stonehouse NJ Virus Assembly and Morphogenesis In Development-genetics, epigentics and environmental regulation , 1999

McGregor A; Murray JB; Adams CJ; Stockley PG; Connolly BA Secondary structure mapping of an RNA ligand that has high affinity for the MetJ repressor protein and interference modification analysis of the protein-RNA complex J BIOL CHEM 274 2255-2262, 1999

Stonehouse NJ Dissecting the key recognition features of the MS2 bacteriophage translational repression complex Nucleic Acids Research 26 1337-1344, 1998

Stockley PG Dissecting the molecular details of prokaryotic transcriptional control by surface plasmon resonance: the methionine and arginine repressor proteins Biosensors and Bioelectronics 13 637-650, 1998

Stockley PG Dissecting the key recognition features of the MS2 bacteriophage translational repression complex Nucleic Acids Research 26 1337-1344, 1998

van den Worm SHE; Stonehouse NJ; Valegard K; Murray JB; Walton C; Fridborg K; Stockley PG Crystal structures of MS2 coat protein mutants in complex with wild-type RNA operator fragments Nucleic Acids Research 26 1345-1351, 1998

Stonehouse NJ; Phillips SEV; Stockley PG; Convery MA; Rowsell S; Murray JB; Hirao I; Ellington AD; Peabody DS Crystal structure of an RNA aptamer protein complex at 2.8 angstrom resolution Nature Structural Biology 5 133-139, 1998
View abstract

Rowsell S; Stonehouse NJ; Convery MA; Adams CJ; Ellington AD; Hirao I; Peabody DS; Stockley PG; Phillips SE Crystal structures of a series of RNA aptamers complexed to the same protein target. Nat Struct Biol 5 970-975, 1998
DOI:10.1038/2946
View abstract

Stonehouse NJ The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions Journal of Molecular Biology 270 724-738, 1997

Stockley PG The three-dimensional structures of two complexes between recombinant MS2 capsids and RNA operator fragments reveal sequence-specific protein-RNA interactions Journal of Molecular Biology 270 724-738, 1997

Stockley PG Quantitation of the Escherichia coli methionine repressor-operator interaction by the surface plasmon resonance is not affected by the presence of a dextran matrix Unknown Journal - Requested journal to be added to ULPD 254 82-87, 1997

Stockley PG Analysis of phage MS2 coat protein mutants expressed from a reconstituted phagemid reveals that proline 78 is essential for viral infectivity Journal of Molecular Biology 266 1-7, 1997

Kim CH; Murray JB; Adams CJ; Stockley PG; Abelson J Probing the active site of the yeast spliceosome The FASEB Journal 11 23-23, 1997

Miller CM; Baumberg S; Stockley PG Operator interactions by the Bacillus subtilis arginine repressor/activator, AhrC: novel positioning and DNA-mediated assembly of a transcriptional activator at catabolic sites Molecular Microbiology 26 37-48, 1997
View abstract

Stockley PG Operator interactions by the Bacillus subtilis arginine repressor/activator, AhrC: novel positioning and DNA-mediated assembly of a transcriptional activator at catabolic sites Molecular Microbiology 24 37-48, 1997

Stockley PG Specific cytotoxicity against cells bearing HIV1 gp120 antigen by bacteriophage-encapsidated ricin A chain: Implications for cell specific drug delivery Biochemical Society Transactions 25 158-158, 1997

Wu M; Brown WL; Hill HR; Stockley PG Specific cytotoxicity against cells bearing HIV1 gp120 antigen by bacteriophage-encapsidated ricin A chain: implications for cell specific drug delivery. Biochem Soc Trans 25 158S-, 1997

Kim CH; Adams CJ; Abelson J; Murray JB; Stockley PG Probing the active site of the yeast spliceosome.With the site-specifically derivated 5'ss RNA FASEB Journal 11 -, 1997
View abstract

Stockley PG Preparation of Oligoribonucleotides containg 4-Thiouridine using FPMP chemistry - photo-cross-linking to RNA-binding proteins using 350 NM irradiation Nucleic Acids Research 24 3173-3180, 1996

Stockley PG Molecular-interactions in the RNA bacteriophage-MS2 Biochemical Society Transactions 24 412-412, 1996

Stockley PG Structure and function of Escherichia-Coli met repressor-similarities and contrasts with TRP repressor Unknown Journal - Requested journal to be added to ULPD 351 527-535, 1996

Stockley PG Effects of systematic variation of the minimul Escherichia-Coli met consensus operator site- in-vivo and in-vitro met repressor binding Molecular Microbiology 21 1125-1135, 1996

Stockley PG Phage presentation Molecular Microbiology 20 685-692, 1996

Stockley PG Development of a novel drug-delivery system using Bacteriophage-MS2 capsids Unknown Journal - Requested journal to be added to ULPD 24 413-413, 1996

Stonehouse NJ; Valegard K; Golmohammadi R; van den Worm SHE; Walton C; Stockley PG; Liljas L Crystal-structures of MS2 capsids with mutations in the subunit FG loop Journal of Molecular Biology 256 330-339, 1996

Stockley PG Biomolecular interaction analysis Unknown Journal - Requested journal to be added to ULPD 14 39-41, 1996

Stockley PG Cell-specific delivery of drugs via bacteriophage-MS2 synthetic virions (SVS) Journal of Pathology 179 38-38, 1996

He YY; Stockley PG; Gold L In-vitro evolution of the DNA-binding sites of Escherichia-Coli Methionine repressor, METJ Journal of Molecular Biology 255 55-66, 1996
View abstract

Stonehouse NJ; Scott DJ; Fonseca S; Murray J; Adams C; Clarke AR; Valegård K; Golmohammadi R; van den Worm S; Liljas L; Stockley PG Molecular interactions in the RNA bacteriophage MS2. Biochem Soc Trans 24 412S-, 1996

Wu M; Brown WL; Hill HR; Stockley PG Development of a novel drug-delivery system using bacteriophage MS2 capsids. Biochem Soc Trans 24 413S-, 1996

Stockley PG Probing the molecular mechanism of action of co-repressor in the E.coli methionine repressor-operator complex using surface plasmon resonance Nucleic Acids Res 23 211-216., 1995

Klingel U; Miller CM; North AK; Stockley PG; Baumberg S A binding site for activation by the Bacillus subtilis AhrC protein, a repressor activator of arginine metabolism. Molecular&General Genetics 248(3) 329-340., 1995

Arnold JRP The role of conserved pyrimidine bases in hammerhead ribozyme catalysis: Evidence for a magnesium binding site Biochemical Journal, Portland Press 311 487-494, 1995

Knight CD Molecular responses to abscisic acid and stress are conserved between mosses and cereals The Plant Cell 7 499-506., 1995

Knight CD; Sehgal A; Atwal K; Wallace JC; Cove DJ; Coates D; Quatrano RS; Bahadur S; Stockley PG; Cuming AC Molecular Responses to Abscisic Acid and Stress Are Conserved between Moss and Cereals. Plant Cell 7 499-506, 1995
DOI:10.1105/tpc.7.5.499
View abstract

Murray JB; Adams CJ; Arnold JR; Stockley PG The roles of the conserved pyrimidine bases in hammerhead ribozyme catalysis: evidence for a magnesium ion-binding site. Biochem J 311 ( Pt 2) 487-494, 1995
View abstract

Stockley PG; Stonehouse NJ; Murray JB; Goodman ST; Talbot SJ; Adams CJ; Liljas L; Valegård K Probing sequence-specific RNA recognition by the bacteriophage MS2 coat protein. Nucleic Acids Res 23 2512-2518, 1995
View abstract

Renwick SB; Critchley AD; Adams CJ; Kelly SM; Price NC; Stockley PG Probing the details of the HIV-1 Rev-Rev-responsive element interaction: effects of modified nucleotides on protein affinity and conformational changes during complex formation. Biochem J 308 ( Pt 2) 447-453, 1995
View abstract

Wu M; Brown WL; Stockley PG Cell-specific delivery of bacteriophage-encapsidated ricin A chain. Bioconjug Chem 6 587-595, 1995
View abstract

ADAMS CJ; MURRAY JB; FARROW MA; ARNOLD JRP; STOCKLEY PG INCORPORATION OF 6-THIOGUANOSINE INTO OLIGORIBONUCLEOTIDES TETRAHEDRON LETT 36 5421-5424, 1995

ADAMS CJ; FARROW MA; MURRAY JB; KELLY SM; PRICE NC; STOCKLEY PG INCORPORATION OF 6-THIOINOSINE INTO OLIGORIBONUCLEOTIDES TETRAHEDRON LETT 36 4637-4640, 1995

KNOWLES PF; STOCKLEY PG LESSONS FROM NATURE CHEM BRIT 31 27-&, 1995

Arnold JRP A convenient synthesis of s-cyanoethyl-protected 2-thiouridine and its incorporation into oligoribonucleotides Tetrahedron Letters, Elsevier 35 765-768, 1994

Stockley PG Crystal structure of the MS2 coat protein-operator complex at 3.0Å resolution. Nature 371 623-626., 1994

Stockley PG; Stonehouse NJ; Valegård K Molecular mechanism of RNA phage morphogenesis. Int J Biochem 26 1249-1260, 1994
View abstract

Manfield I; Stockley PG Ethylation interference. Methods Mol Biol 30 125-139, 1994
DOI:10.1385/0-89603-256-6:125

Stockley PG Filter-binding assays. Methods Mol Biol 30 251-262, 1994
DOI:10.1385/0-89603-256-6:251

VALEGARD K; MURRAY JB; STOCKLEY PG; STONEHOUSE NJ; LILJAS L CRYSTAL-STRUCTURE OF AN BACTERIOPHAGE-RNA COAT PROTEIN-OPERATOR COMPLEX NATURE 371 623-626, 1994

COOPER A; MCALPINE A; STOCKLEY PG CALORIMETRIC STUDIES OF THE ENERGETICS OF PROTEIN-DNA INTERACTIONS IN THE ESCHERICHIA-COLI METHIONINE REPRESSOR (METJ) SYSTEM FEBS LETT 348 41-45, 1994

ADAMS CJ; MURRAY JB; ARNOLD JRP; STOCKLEY PG INCORPORATION OF A FLUORESCENT NUCLEOTIDE INTO OLIGORIBONUCLEOTIDES TETRAHEDRON LETT 35 1597-1600, 1994

PHILLIPS SEV; STOCKLEY PG SIMILARITY OF MET AND TRP REPRESSORS NATURE 368 106-106, 1994

ADAMS CJ; MURRAY JB; ARNOLD JRP; STOCKLEY PG A CONVENIENT SYNTHESIS OF S-CYANOETHYL-PROTECTED 4-THIOURIDINE AND ITS INCORPORATION INTO OLIGORIBONUCLEOTIDES TETRAHEDRON LETT 35 765-768, 1994

Stockley PG; Stonehouse NJ; Walton C Molecular mechanism of RNA Phage Morphogenesis Biochemical Society Transactions 21 627-633, 1993

Mastico RA; Talbot SJ; Stockley PG Multiple presentation of foreign peptides on the surface of an RNA-free spherical bacteriophage capsid. J Gen Virol 74 ( Pt 4) 541-548, 1993
View abstract

Stonehouse NJ; Stockley PG Effects of amino acid substitution on the thermal stability of MS2 capsids lacking genomic RNA. FEBS Lett 334 355-359, 1993
View abstract

Critchley AD; Haneef I; Cousens DJ; Stockley PG Modeling and solution structure probing of the HIV-1 TAR stem-loop. J Mol Graph 11 92-124, 1993
View abstract

Baumberg S Purification and initial characterization of AhrC - the regulator of arginine metabolism genes in Bacillus subtilis. Molecular Microbiology 6(2) 267-275., 1992

Czaplewski LG; North AK; Smith MC; Baumberg S; Stockley PG Purification and initial characterization of AhrC: the regulator of arginine metabolism genes in Bacillus subtilis. Mol Microbiol 6 267-275, 1992
View abstract

He YY; McNally T; Manfield I; Navratil O; Old IG; Phillips SE; Saint-Girons I; Stockley PG Probing met repressor-operator recognition in solution. Nature 359 431-433, 1992
DOI:10.1038/359431a0
View abstract

JOHNSON CM; COOPER A; STOCKLEY PG DIFFERENTIAL SCANNING CALORIMETRY OF THERMAL UNFOLDING OF THE METHIONINE REPRESSOR PROTEIN (METJ) FROM ESCHERICHIA-COLI BIOCHEMISTRY-US 31 9717-9724, 1992

TILEY LS; MALIM MH; TEWARY HK; STOCKLEY PG; CULLEN BR IDENTIFICATION OF A HIGH-AFFINITY RNA-BINDING SITE FOR THE HUMAN-IMMUNODEFICIENCY-VIRUS TYPE-1 REV PROTEIN P NATL ACAD SCI USA 89 758-762, 1992

Stockley PG Viral protein-nucleic acid interactions Current Opinion in Structural Biology 2 143-149, 1992
View abstract

Walton C; Booth RK; Stockley PG Random chemical mutagenesis and non-selective isolation of mutated DNA sequences in vitro In Directed Mutagenesis: A Practical Approach , 1991

Talbot SJ; Medina G; Fishwick CW; Haneef I; Stockley PG Hyperreactivity of adenines and conformational flexibility of a translational repression site. FEBS Lett 283 159-164, 1991
View abstract

Goodman ST; Talbot SJ; Haneef I; Tewary HK; Fishwick CW; Stockley PG Synthetic RNA in the study of RNA-protein interactions of MS2 bacteriophage. Nucleic Acids Symp Ser 293-, 1991

OLD IG; PHILLIPS SEV; STOCKLEY PG; SAINTGIRONS I REGULATION OF METHIONINE BIOSYNTHESIS IN THE ENTEROBACTERIACEAE PROG BIOPHYS MOL BIO 56 145-185, 1991

Talbot SJ; Goodman S; Bates SR; Fishwick CW; Stockley PG Use of synthetic oligoribonucleotides to probe RNA-protein interactions in the MS2 translational operator complex. Nucleic Acids Res 18 3521-3528, 1990
View abstract

Boys CW; Czaplewski LG; Phillips SE; Baumberg S; Stockley PG Crystallization of the arginine-dependent repressor/activator AhrC from Bacillus subtilis. J Mol Biol 213 227-228, 1990
DOI:10.1016/S0022-2836(05)80185-8
View abstract

Haneef I; Talbot SJ; Stockley PG Modeling loop structures in proteins and nucleic acids: an RNA stem-loop. J Mol Graph 7 186-195, 1989
View abstract

Boys CW; Hill DJ; Stockley PG; Woodward JR Crystallization of alcohol oxidase from Pichia pastoris. J Mol Biol 208 211-212, 1989
View abstract

Phillips SE; Manfield I; Parsons I; Davidson BE; Rafferty JB; Somers WS; Margarita D; Cohen GN; Saint-Girons I; Stockley PG Cooperative tandem binding of met repressor of Escherichia coli. Nature 341 711-715, 1989
DOI:10.1038/341711a0
View abstract

SMITH MCM; CZAPLEWSKI L; NORTH AK; BAUMBERG S; STOCKLEY PG SEQUENCES REQUIRED FOR REGULATION OF ARGININE-BIOSYNTHESIS PROMOTERS ARE CONSERVED BETWEEN BACILLUS-SUBTILIS AND ESCHERICHIA-COLI MOL MICROBIOL 3 23-28, 1989

CARRINGTON JC; MORRIS TJ; STOCKLEY PG; HARRISON SC STRUCTURE AND ASSEMBLY OF TURNIP CRINKLE VIRUS .4. ANALYSIS OF THE COAT PROTEIN GENE AND IMPLICATIONS OF THE SUBUNIT PRIMARY STRUCTURE J MOL BIOL 194 265-276, 1987

SORGER PK; STOCKLEY PG; HARRISON SC STRUCTURE AND ASSEMBLY OF TURNIP CRINKLE VIRUS .2. MECHANISM OF REASSEMBLY INVITRO J MOL BIOL 191 639-658, 1986

STOCKLEY PG; KIRSH AL; CHOW EP; SMART JE; HARRISON SC STRUCTURE OF TURNIP CRINKLE VIRUS .3. IDENTIFICATION OF A UNIQUE COAT PROTEIN DIMER J MOL BIOL 191 721-725, 1986

STOCKLEY PG; THOMAS JO NUCLEOSOME-LIKE PARTICLE CONTAINING AN OCTAMER OF THE ARGININE-RICH HISTONES H-3 AND H-4 FEBS LETT 99 129-135, 1979

Joce C; Caryl JA; Stockley PG; Warriner S; Nelson A Identification of stable S-adenosylmethionine (SAM) analogues derivatised with bioorthogonal tags: effect of ligands on the affinity of the E. coli methionine repressor, MetJ, for its operator DNA Organic&Biomolecular Chemistry 7 635-638,
DOI:10.1039/b816495a