Faculty of Biological Sciences

Prof Asipu Sivaprasadarao

BSc, PhD 1987, Leeds.
Professor of Membrane Biology
School of Biomedical Sciences

Background: BSc, MSc (Andhra University, Visakhapatnam, India); PhD (Leeds; Commonwealth Fellow); Professor of Membrane Biology

Contact:  Garstang G6.44d, +44(0) 113 34 34326, email address for  

You can read more about Prof Sivaprasadarao's interests here:
www.fbs.leeds.ac.uk/staff/Sivaprasadarao_A/
Integrative Membrane Biology


Research Interests

Research theme: Ion channels and Human Diseases

Aging is a major risk factor for most late-onset illnesses including diabetes, cancer, cardiovascular (high blood pressure, heart attack) and neuronal (stroke, Alzheimer’s and Parkinson’s) diseases. Interestingly, many of the diseases are interconnected- for example, diabetes is strongly associated with the risk of cardiovascular and neuronal disorders.

At the cellular level, aging leads to decline in mitochondrial health, resulting in increased production of reactive oxygen species and reduced production of ATP. These cellular changes affect the health of individual tissues and the quality of life as we age.

Our overarching goal is to understand the mechanism(s) by which reactive oxygen species affect the structure and function of mitochondria. The idea is to translate this knowledge to develop new medicines to improve the quality of life as we grow older. The focus will be on how ion channels regulate mitochondrial biology and disease outcome.

Our strategy is to build on our long-standing experience in the appreciation of cell biological and molecular basis of human diseases. We will combine the traditional cell biological and molecular approaches with emerging technologies (super-resolution microscopy; CRISPR/Cas9) and in vivo (transgenic) methods. With medicinal chemists we are developing new chemicals to prevent diseases such as diabetes.

Collaborators: We aim to achieve our goals through multi-disciplinary collaborative research.

For diabetes, we collaborate with clinical scientists: Prof. MT Kearney, Prof. DJ Beech, and Dr SB Wheatcroft.

For Alzheimer’s and Parkinson’s diseases, we collaborate with Dr Lin-Hua Jiang, Dr James Duce and Dr Stephen Clapcote.

For drug discovery research, we collaborate with the medicinal chemist, Dr Richard Foster.

For structural biology of ion channels, we collaborate with Prof. A. Goldman and Dr S Muench

Techniques to be used:

General molecular cloning techniques; Immunochemistry: confocal and super-resolution microscopy, live cell imaging, calcium imaging; in vivo techniques, including transgenics; protein expression and purification.

Funding: We thank the Wellcome Trust, MRC, British heart Foundation, GSK, and Millipore for supporting our research.

 

Current Projects

  1. Role of ion channels in Parkinson’s and Alzheimer’s disease.
  2. Regulation of mitochondrial dynamics in cardiovascular diseases
  3. Developing novel therapeutics to treat diabetes
  4. Role of ion channels in cancer cell migration

 

Applications are invited for Masters by Research and PhD degree programmes in the above topics. 

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Ion channels in human diseases, including diabetes, Parkinson's and cancer

Postgraduate studentship areas:

  • Role of ion channels in mitochondrial dynamics and human diseases (diabetes, cardiovascular and neuronal).

See also:

Modules managed

BMSC2230 - Topics in Pharmacology

Modules taught

BIOW5906X - Theory, computation and bioinformatics
BMSC1110 - Foundations of Biomedical Sciences
BMSC1212 - Introduction to Pharmacology
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2120 - Scientific Skills
BMSC2230 - Topics in Pharmacology
BMSC3101 - Inherited Disorders
BMSC3143/44/45/46 - Advanced topics for BMS students I
BMSC3233/34/35/36 - Advanced topics for BMS students II
BMSC3301 - Research Project in Biomedical Sciences
BMSC3399 - Extended Research Project Preparation

Centre membership: The Multidisciplinary Cardiovascular Research Centre (MCRC)

Postgraduates

Maali Alahmad (Primary supervisor) 100% FTE
Anthony Chan (Primary supervisor) 70% FTE
Andrew Tsatsanis (Primary supervisor) 50% FTE
Matthias Gantner (Co-supervisor) 50% FTE
Sophie Hesketh (Co-supervisor) 50% FTE
Xin Li (Co-supervisor) 25% FTE

Abuarab N, Munsey TS, Jiang LH, Li J, Sivaprasadarao A High glucose-induced ROS activates TRPM2 to trigger lysosomal membrane permeabilization and Zn<sup>2+</sup>-mediated mitochondrial fission Science Signaling 10 -, 2017
DOI:10.1126/scisignal.aal4161
View abstract

Li F, Munsey TS, Sivaprasadarao A TRPM2-mediated rise in mitochondrial Zn(2+) promotes palmitate-induced mitochondrial fission and pancreaticβ-cell death in rodents. Cell Death Differ -, 2017
DOI:10.1038/cdd.2017.118
View abstract

Li F, Abuarab N, Sivaprasadarao A Reciprocal regulation of actin cytoskeleton remodelling and cell migration by Ca 2+ and Zn 2+ : role of TRPM2 channels Development 143 e1.1-e1.1, 2016
DOI:10.1242/dev.139881

Li F, Abuarab N, Sivaprasadarao A Reciprocal regulation of actin cytoskeleton remodelling and cell migration by Ca<sup>2+</sup>and Zn<sup>2+</sup>: Role of TRPM2 channels Journal of Cell Science 129 2016-2029, 2016
DOI:10.1242/jcs.179796
View abstract

Martin DSD, Leonard S, Devine R, Redondo C, Kinsella GK, Breen CJ, McEneaney V, Rooney MF, Munsey TS, Porter RK, Sivaprasadarao A, Stephens JC, Findlay JBC Novel mitochondrial complex I inhibitors restore glucose-handling abilities of high-fat fed mice Journal of Molecular Endocrinology 56 261-271, 2016
DOI:10.1530/JME-15-0225
View abstract

Manna PT, Munsey TS, Abuarab N, Li F, Asipu A, Howell G, Sedo A, Yang W, Naylor J, Beech DJ, Jiang LH, Sivaprasadarao A TRPM2-mediated intracellular Zn&lt;sup&gt;2+&lt;/sup&gt; release triggers pancreaticβ-cell death Biochemical Journal 466 537-546, 2015
DOI:10.1042/BJ20140747
View abstract

Li F, Manna PT, Sivaprasadarao A Reciprocal regulation of actin remodelling and cell migration by Calcium and Zinc: role of H2O2 and TRPM2 channels MOLECULAR BIOLOGY OF THE CELL 25 -, 2014

Jopling HM, Odell AF, Pellet-Many C, Latham AM, Frankel P, Sivaprasadarao A, Walker JH, Zachary IC, Ponnambalam S Endosome-to-Plasma Membrane Recycling of VEGFR2 Receptor Tyrosine Kinase Regulates Endothelial Function and Blood Vessel Formation. Cells 3 363-385, 2014
DOI:10.3390/cells3020363
View abstract

Dhillon MS, Cockcroft CJ, Munsey T, Smith KJ, Powell AJ, Carter P, Wrighton DC, Rong H-L, Yusaf SP, Sivaprasadarao A A functional Kv1.2-hERG chimaeric channel expressed in Pichia pastoris SCIENTIFIC REPORTS 4 -, 2014
DOI:10.1038/srep04201

Ye M, Yang W, Ainscough JF, Hu XP, Li X, Sedo A, Zhang XH, Zhang X, Chen Z, Li XM, Beech DJ, Sivaprasadarao A, Luo JH, Jiang LH TRPM2 channel deficiency prevents delayed cytosolic Zn&lt;sup&gt;2+&lt;/sup&gt; accumulation and CA1 pyramidal neuronal death after transient global ischemia Cell Death and Disease 5 -, 2014
DOI:10.1038/cddis.2014.494
View abstract

Jin X, Shah S, Liu Y, Zhang H, Lees M, Fu Z, Lippiat JD, Beech DJ, Sivaprasadarao A, Baldwin SA, Zhang H, Gamper N Activation of the Cl- channel ANO1 by localized calcium signals in nociceptive sensory neurons requires coupling with the IP3 receptor. Sci Signal 6 ra73-, 2013
DOI:10.1126/scisignal.2004184
View abstract

Zou J, Ainscough JF, Yang W, Sedo A, Yu S-P, Mei Z-Z, Sivaprasadarao A, Beech DJ, Jiang L-H A differential role of macrophage TRPM2 channels in Ca2+ signaling and cell death in early responses to H2O2 AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY 305 C61-C69, 2013
DOI:10.1152/ajpcell.00390.2012

Sierra A, Zhu Z, Sapay N, Sharotri V, Kline CF, Luczak ED, Subbotina E, Sivaprasadarao A, Snyder PM, Mohler PJ, Anderson ME, Vivaudou M, Zingman LV, Hodgson-Zingman DM Regulation of cardiac ATP-sensitive potassium channel surface expression by calcium/calmodulin-dependent protein kinase II Journal of Biological Chemistry 288 1568-1581, 2013
DOI:10.1074/jbc.M112.429548
View abstract

Karnik R, Ludlow MJ, Abuarab N, Smith AJ, Hardy MEL, Elliott DJS, Sivaprasadarao A Endocytosis of HERG is clathrin-independent and involves arf6. PLoS One 8 e85630-, 2013
DOI:10.1371/journal.pone.0085630
View abstract

Elliott DJS, Neale EJ, Munsey TS, Bannister JP, Sivaprasadarao A Role of hydrophobic and ionic forces in the movement of S4 of the Shaker potassium channel. Mol Membr Biol 29 321-332, 2012
DOI:10.3109/09687688.2012.710343
View abstract

Yang W, Manna PT, Zou JE, Luo JH, Beech DJ, Sivaprasadarao A, Jiang LH Zinc Inactivates Melastatin Transient Receptor Potential 2 Channels via the Outer Pore J BIOL CHEM 286 23789-23798, 2011
DOI:10.1074/jbc.M111.247478

Bradley HJ, Baldwin JM, Goli GR, Johnson B, Zou J, Sivaprasadarao A, Baldwin SA, Jiang LH Residues 155 and 348 Contribute to the Determination of P2X(7) Receptor Function via Distinct Mechanisms Revealed by Single-nucleotide Polymorphisms J BIOL CHEM 286 8176-8187, 2011
DOI:10.1074/jbc.M110.211284

Manna PT, Smith AJ, Taneja TK, Howell GJ, Lippiat JD, Sivaprasadarao A Constitutive Endocytic Recycling and Protein Kinase C-mediated Lysosomal Degradation Control K-ATP Channel Surface Density J BIOL CHEM 285 5963-5973, 2010
DOI:10.1074/jbc.M109.066902

Manna PT, Smith AJ, Fletcher S, Sivaprasadarao A Differential PKC Isoform Regulation of K-ATP Channel Trafficking and Function BIOPHYSICAL JOURNAL 98 698A-698A, 2010

Karnik R, Smith AJ, Elliott DJ, Taneja TK, Aviss K, Sivaprasadarao A Lipid Raft and Arf6-GTPase Dependent Endocytosis of the hERG Potassium Channel BIOPHYSICAL JOURNAL 98 375A-375A, 2010

Elliott DJS, Dondas NY, Munsey TS, Sivaprasadarao A Movement of the S4 segment in the hERG potassium channel during membrane depolarization MOL MEMBR BIOL 26 435-447, 2009
DOI:10.3109/09687680903321081

Taneja TK, Mankouri J, Karnik R, Kannan S, Smith AJ, Munsey T, Christesen HBT, Beech DJ, Sivaprasadarao A Sar1-GTPase-dependent ER exit of K-ATP channels revealed by a mutation causing congenital hyperinsulinism HUM MOL GENET 18 2400-2413, 2009
DOI:10.1093/hmg/ddp179

Amer MS, Li J, O'Regan DJ, Steele DS, Porter KE, Sivaprasadarao A, Beech DJ Translocon closure to Ca2+ leak in proliferating vascular smooth muscle cells AM J PHYSIOL-HEART C 296 H910-H916, 2009
DOI:10.1152/ajpheart.00984.2008

Amer MS, Li J, Regan DO, Porter KE, Sivaprasadarao A, Beech DJ TRANSLOCON CLOSURE TO CALCIUM LEAK IN PROLIFERATING VASCULAR SMOOTH MUSCLE CELLS J PHYSIOL SCI 59 239-239, 2009

Carr R, Weinstock IA, Sivaprasadarao A, Muller A, Aksimentiev A Synthetic Ion Channels via Self-Assembly: A Route for Embedding Porous Polyoxometalate Nanocapsules in Lipid Bilayer Membranes NANO LETT 8 3916-3921, 2008
DOI:10.1021/nl802366k

Sukumar P, Xu S-Z, Zeng F, Li J, Bahnasi YM, Jiang L-H, Sivaprasadarao A, Beech DJ Functional cysteine pair in TRPC5 cation channel FASEB JOURNAL 22 -, 2008

Xu SZ, Sukumar P, Zeng FN, Li J, Jairaman A, English A, Naylor J, Ciurtin C, Majeed Y, Milligan CJ, Bahnasi YM, Al-Shawaf E, Porter KE, Jiang LH, Emery P, Sivaprasadarao A, Beech DJ TRPC channel activation by extracellular thioredoxin NATURE 451 69-U9, 2008
DOI:10.1038/nature06414

Smith AJ, Sivaprasadarao A Investigation of K(ATP) channel endocytosis and cell surface density by Biotinylation and Western blotting. Methods Mol Biol 491 79-89, 2008
DOI:10.1007/978-1-59745-526-8_6
View abstract

Smith AJ, Sivaprasadarao A Investigation of K(ATP) channel endocytosis by immunofluorescence. Methods Mol Biol 491 69-77, 2008
DOI:10.1007/978-1-59745-526-8_5
View abstract

Smith AJ, Sivaprasadarao A Chemiluminescence assays to investigate membrane expression and clathrin-mediated endocytosis of K(ATP) channels. Methods Mol Biol 491 63-68, 2008
DOI:10.1007/978-1-59745-526-8_4
View abstract

Neale EJ, Rong HL, Cockcroft CJ, Sivaprasadarao A Mapping the membrane-aqueous border for the voltage-sensing domain of a potassium channel J BIOL CHEM 282 37597-37604, 2007

Sivaprasadarao A, Taneja TK, Mankouri J, Smith AJ Trafficking of ATP-sensitive potassium channels in health and disease BIOCHEMICAL SOCIETY TRANSACTIONS 35 1055-1059, 2007
DOI:10.1042/BST0351055

Smith AJ, Taneja TK, Mankouri J, Sivaprasadarao A Molecular cell biology of KATP channels: implications for neonatal diabetes. Expert Reviews in Molecular Medicine 9 1-17, 2007

Smith AJ, Partridge CJ, Asipu A, Mair LA, Hunter M, Sivaprasadarao A Increased ATP-sensitive K+ channel expression during acute glucose deprivation BIOCHEM BIOPH RES CO 348 1123-1131, 2006
DOI:10.1016/j.bbrc.2006.07.170

Mankouri J, Taneja TK, Smith AJ, Ponnambalam S, Sivaprasadarao A Kir6.2 mutations causing neonatal diabetes prevent endocytosis of ATP-sensitive potassium channels EMBO J 25 4142-4151, 2006
DOI:10.1038/sj.emboj.7601275

Morton MJ, Abohamed A, Sivaprasadarao A, Hunter M pH sensing in the two-pore domain K+ channel, TASK2 P NATL ACAD SCI USA 102 16102-16106, 2005
DOI:10.1073/pnas.0506870102

Xu S-Z, Zeng F, Lei M, Li J, Gao B, Xiong C, Sivaprasadarao A, Beech DJ Generation of functional ion-channel tools by E3 targeting. Nat Biotechnol 23 1289-1293, 2005
DOI:10.1038/nbt1148
View abstract

Hussain K, Cosgrove KE, Shepherd RM, Luharia A, Smith VV, Kassem S, Gregory JW, Sivaprasadarao A, Christesen HT, Jacobsen BB, Brusgaard K, Glaser B, Maher EA, Lindley KJ, Hindmarsh P, Dattani M, Dunne MJ Hyperinsulinemic hypoglycemia in Beckwith-Wiedemann syndrome due to defects in the function of pancreatic beta-cell adenosine triphosphate-sensitive potassium channels. J Clin Endocrinol Metab 90 4376-4382, 2005
DOI:10.1210/jc.2005-0158
View abstract

O'Connell AD, Morton MJ, Sivaprasadarao A, Hunter M Selectivity and interactions of Ba2+ and Cs+ with wild-type and mutant TASK1 K+ channels expressed in Xenopus oocytes. J Physiol 562 687-696, 2005
DOI:10.1113/jphysiol.2004.079020
View abstract

Morton MJ, Chipperfield S, Abohamed A, Sivaprasadarao A, Hunter M Na+-induced inward rectification in the two-pore domain K+ channel, TASK-2 AM J PHYSIOL-RENAL 288 F162-F169, 2005
DOI:10.1152/ajprenal.00248.2004

Elliott DJS, Neale EJ, Aziz Q, Dunham JP, Munsey TS, Hunter M, Sivaprasadarao A Molecular mechanism of voltage sensor movements in a potassium channel EMBO J 23 4717-4726, 2004
DOI:10.1038/sj.emboj.7600484

Fountain SJ, Cheong A, Flemming R, Mair L, Sivaprasadarao A, Beech DJ Functional up-regulation of KCNA gene family expression in murine mesenteric resistance artery smooth muscle Journal of Physiology 556 29-42, 2004

Cobbold C, Monaco AP, Sivaprasadarao A, Ponnambalam S Aberrant trafficking of transmembrane proteins in human disease. Trends in Cell Biology 13 639-647, 2003
DOI:10.1016/j.tcb.2003.10.008

Neale EJ, Elliott DJS, Hunter M, Sivaprasadarao A Evidence for intersubunit interactions between S4 and S5 transmembrane segments of the Shaker potassium channel J BIOL CHEM 278 29079-29085, 2003
DOI:10.1074/M301991200/6493

Neale EJ, Elliott DJS, Hunter M, Sivaprasadarao A Evidence for intersubunit interactions between S4 and S5 transmembrane segments of the Shaker potassium channel. J Biol Chem 278 29079-29085, 2003
DOI:10.1074/jbc.M301991200/6493
View abstract

Morton MJ, O'Connell AD, Sivaprasadarao A, Hunter M Determinants of pH sensing in the two-pore domain K+ channels TASK-1 and-2 PFLUG ARCH EUR J PHY 445 577-583, 2003
DOI:10.1007/s00424-002-0901-2

Claydon TW, Boyett MR, Sivaprasadarao A, Orchard CH Two pore residues mediate acidosis-induced enhancement of C-type inactivation of the Kv1.4 K+ channel AM J PHYSIOL-CELL PH 283 C1114-C1121, 2002
DOI:10.1152/ajpcell.00542.2001

Munsey TS, Mohindra A, Yusaf SP, Grainge A, Wang M-H, Wray D, Sivaprasadarao A Functional properties of Kch, a prokaryotic homologue of eukaryotic potassium channels. Biochem Biophys Res Commun 297 10-16, 2002
View abstract

Aziz QH, Partridge CJ, Munsey TS, Sivaprasadarao A Depolarization Induces Intersubunit Cross-linking in a S4 Cysteine Mutant of the Shaker Potassium Channel Journal of Biological Chemistry 277 42719-42725, 2002
DOI:10.1074/jbc.M207258200

Morton MJ, O'Connell AD, Garside E, Sivaprasadarao A, Hunter M pH sensing in the two-pore domain K+ channel TASK2 expressed in Xenopus oocytes J PHYSIOL-LONDON 539 16P-17P, 2002

O'Connell AD, Morton MJ, Sivaprasadarao A, Hunter M Barium sensitivity of TASK-1 (KCNK-3) mutants expressed in Xenopus oocytes J PHYSIOL-LONDON 539 16P-16P, 2002

Hough H, Mair L, Mackenzie W, Sivaprasadarao A Expression, purification and evidence for the interaction of the two nucleotide-binding folds of the sulphonylurea receptor. Biochemical and Biophysical Research Communications 294 191-197, 2002
DOI:10.1016/S0006-291X(02)00454-0

Sundaram M, van Aalten DMF, Findlay JBC, Sivaprasadarao A The transfer of transthyretin and receptor-binding properties from the plasma retinol-binding protein to the epididymal retinoic acid-binding protein. Biochemical Journal 362 265-271, 2002
DOI:10.1042/0264-6021:3620265

Partridge CJ, Beech DJ, Sivaprasadarao A Identification and Pharmacological Correction of a Membrane Trafficking Defect Associated with a Mutation in the Sulfonylurea Receptor Causing Familial Hyperinsulinism Journal of Biological Chemistry 276 35947-35952, 2001
DOI:10.1074/jbc.M104762200

Smith JA, Sivaprasadarao A, Munsey TS, Bowmer CJ, Yates MS Immunolocalisation of adenosine A(1) receptors in the rat kidney. Biochem Pharmacol 61 237-244, 2001
View abstract

Claydon TW, Boyett MR, Sivaprasadarao A, Leach R, Orchard CH Modification of an extracellular residue by MTSET mimics the effect of acidosis on the Kv1.4 K+ channel BIOPHYS J 80 443A-444A, 2001

Aziz QH, Partridge CJ, Hunter M, Sivaprasadarao A Engineered disulphides between the S4 segments of the Shaker potassium channel stabilises the open conformational state BIOPHYS J 80 218A-218A, 2001

Claydon T, Boyett MR, Sivaprasadarao A, Ishii K, Owen JM, O'Beirne HA, Leach R, Komukai K, Orchard CH Inhibition of the K+ channel Kv1.4 by acidosis: protonation of an extracellular histidine slows the recover from N-type inactivation Journal of Physiology 526.2 253-264, 2000

Hough E, Beech DJ, Sivaprasadarao A Identification of Molecular Regions Responsible for Membrane Trafficking of Kir6.2 Pflugers Archiv: European Journal of Physiology 440 481-487, 2000

Bannister J, Young BA, Sivaprasadarao A, Wray D Conserved extracellular cysteine residues in the inwardly rectifying potassium channel Kir2.3 are required for function but not expression in the membraine. FEBS Letters 458 393-399, 1999

Wang MH, Yusaf SP, Elliott DJS, Wray D, Sivaprasadarao A Effect of cysteine substitutions on the topology of the S4 segment of shaker potassium channel: implications for molecular models of gating. Journal of Physiology 521 315-326, 1999

Bannister JPA, Young BA, Main MJ, Sivaprasadarao A, Wray D The effects of oxidizing and cysteine-reactive reagents on the inward rectifier potassium channels Kir2.3 and Kir1.1 Pflugers Archiv: European Journal of Physiology 438 868-878, 1999

Surah-Narwal S, Xu S, McHugh D, McDonald RL, Hough E, Cheong A, Partridge CJ, Sivaprasadarao A, Beech DJ Block of human aorta Kir6.1 by the vascular K ATP Channel Inhibitor U37883A British Journal of Pharmacology 128 667-672, 1999

Sundaram M, Sivaprasadarao A, Aalten DM, Findlay JB Expression, characterization and engineered specificity of rat epididymal retinoic acid-binding protein. Biochem J 334 ( Pt 1) 155-160, 1998
View abstract

Sundaram M, Sivaprasadarao A, Findlay JB Expression and mutagenesis of retinol-binding protein. Methods Mol Biol 89 141-153, 1998
DOI:10.1385/0-89603-438-0:141

Sivaprasadarao A, Sundaram M, Findlay JB Interactions of retinol-binding protein with transthyretin and its receptor. Methods Mol Biol 89 155-163, 1998
DOI:10.1385/0-89603-438-0:155

Morton MJ, Sivaprasadarao A, Bowmer CJ, Yates MS Adenosine receptor mRNA levels during postnatal renal maturation in the rat Journal of Pharmacy and Pharmacology 50 649-654, 1998

Sundaram M, Sivaprasadarao A, DeSouza MM, Findlay JBC The transfer of retinol from serum retinol-binding protein to cellular retinol-binding is mediated by a membrane receptor Journal of Biological Chemistry 273 3336-3342, 1998

Gould J, Morton MJ, Sivaprasadarao A, Bowmer CJ, Yates MS Renal adenosine A1 receptor binding characteristics and mRNA levels during the development of acute renal failure in the rat. British Journal of Pharmacology 120 947-953, 1997

Boudjelal M, Sivaprasadarao A, Findlay JB Membrane receptor for odour-binding proteins. Biochem J 317 ( Pt 1) 23-27, 1996
View abstract

Jones P, Sivaprasadarao A, Findlay JBC, Wray D A method for determining transmembrane protein structure Molecular Membrane Biology 13 53-60, 1996

Yusaf SP, Wray D, Sivaprasadarao A A measurement of the movement of the S4 segment during the activation of a voltage-gated potassium channel. Pflugers Archiv: European Journal of Physiology 433 91-97, 1996

Carne A, McGregor RA, Bhatia J, Sivaprasadarao A, Keen JN, Davies A, Findlay JB A beta-subclass phosphatidylinositol-specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C-terminus. FEBS Lett 372 243-248, 1995
View abstract

Carne A, McGregor RA, Bhatia J, Sivaprasadarao A, Keen JN, Davies A, Findlay JBC Aβ-subclass phosphatidylinositol-specific phospholipase C from squid (Loligo forbesi) photoreceptors exhibiting a truncated C-terminus FEBS Letters 372 243-248, 1995
DOI:10.1016/0014-5793(95)00936-4
View abstract

Wilson GG, Sivaprasadarao A, Findlay JB, Wray D Changes in activation gating of IsK potassium currents brought about by mutations in the transmembrane sequence. FEBS Lett 353 251-254, 1994
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Wilson GG, O'Neill CA, Sivaprasadarao A, Findlay JB, Wray D Modulation by protein kinase A of a cloned rat brain potassium channel expressed in Xenopus oocytes. Pflugers Arch 428 186-193, 1994
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Sivaprasadarao A, Boudjelal M, Findlay JB Solubilization and purification of the retinol-binding protein receptor from human placental membranes. Biochem J 302 ( Pt 1) 245-251, 1994
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Sivaprasadarao A, Findlay JB Structure-function studies on human retinol-binding protein using site-directed mutagenesis. Biochem J 300 ( Pt 2) 437-442, 1994
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Sivaprasadarao A, Findlay JB Expression of functional human retinol-binding protein in Escherichia coli using a secretion vector. Biochem J 296 ( Pt 1) 209-215, 1993
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Sivaprasadarao A, Boudjelal M, Findlay JB Lipocalin structure and function. Biochem Soc Trans 21 ( Pt 3) 619-622, 1993

Sivaprasadarao A, Findlay JB The interaction of retinol-binding protein with its plasma-membrane receptor. Biochem J 255 561-569, 1988
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Sivaprasadarao A, Findlay JB The mechanism of uptake of retinol by plasma-membrane vesicles. Biochem J 255 571-579, 1988
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Papiz MZ, Sawyer L, Eliopoulos EE, North AC, Findlay JBC, Sivaprasadarao A, Jones TA, Kraulis PJ The structure of beta-lactoglobulin and its similarity to plasma retinol-binding protein. nature 324 383-385, 1986
View abstract

Li F, Abuarab N, Sivaprasadarao A TRPM2: Shredding the mitochondrial network. Channels -,
DOI:10.1080/19336950.2017.1376982