Faculty of Biological Sciences

Prof David Rowlands

BSc (1961), London; PhD (1967), Southampton.
Emeritus Professor of Molecular Virology
School of Molecular and Cellular Biology

Background: 1964-1983 Animal Virus Research Institute, Pirbright; 1983-1995 Wellcome Foundation; 1996-present Professor of Molecular Virology, Division of Microbiology, School of Biochemistry and Molecular Biology, Leeds.

Contact:  Garstang 8.60 | +44(0) 113 34 35641 | email address for Prof David Rowlands 

You can read more about Prof Rowlands's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=DJR
www.fbs.leeds.ac.uk/research/immunology/res_rowlandsd.htm

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Centre membership:

Group Leader Prof David Rowlands  (Emeritus Professor of Molecular Virology)

Replication of positive strand RNA viruses 


Postgraduates

Oluwapelumi Adeyemi  
James Kelly  

Foster TL; Thompson GS; Kalverda AP; Kankanala J; Bentham M; Wetherill LF; Thompson J; Barker AM; Clarke D; Noerenberg M; Pearson AR; Rowlands DJ; Homans SW; Harris M; Foster R; Griffin S Structure-guided design affirms inhibitors of hepatitis C virus p7 as a viable class of antivirals targeting virion release. Hepatology 59 408-422, 2014
DOI:10.1002/hep.26685
View abstract

De Colibus L; Wang X; Spyrou JA; Kelly J; Ren J; Grimes J; Puerstinger G; Stonehouse N; Walter TS; Hu Z; Wang J; Li X; Peng W; Rowlands DJ; Fry EE; Rao Z; Stuart DI More-powerful virus inhibitors from structure-based analysis of HEV71 capsid-binding molecules. Nat Struct Mol Biol 21 282-288, 2014
DOI:10.1038/nsmb.2769
View abstract

Bakker SE; Groppelli E; Pearson AR; Stockley PG; Rowlands DJ; Ranson NA The limits of structural plasticity in a picornavirus capsid revealed by a massively expanded equine rhinitis A virus particle. J Virol -, 2014
DOI:10.1128/JVI.01979-13
View abstract

Shepherd DA; Holmes K; Rowlands DJ; Stonehouse NJ; Ashcroft AE Using ion mobility spectrometry-mass spectrometry to decipher the conformational and assembly characteristics of the hepatitis B capsid protein. Biophys J 105 1258-1267, 2013
DOI:10.1016/j.bpj.2013.07.028
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Ren J; Wang X; Hu Z; Gao Q; Sun Y; Li X; Porta C; Walter TS; Gilbert RJ; Zhao Y; Axford D; Williams M; McAuley K; Rowlands DJ; Yin W; Wang J; Stuart DI; Rao Z; Fry EE Picornavirus uncoating intermediate captured in atomic detail NATURE COMMUNICATIONS 4 -, 2013
DOI:10.1038/ncomms2889

Bentham M; Holmes K; Forrest S; Rowlands DJ; Stonehouse NJ Formation of higher-order foot-and-mouth disease virus 3D(pol) complexes is dependent on elongation activity. J Virol 86 2371-2374, 2012
DOI:10.1128/JVI.05696-11
View abstract

Wang X; Peng W; Ren J; Hu Z; Xu J; Lou Z; Li X; Yin W; Shen X; Porta C; Walter TS; Evans G; Axford D; Owens R; Rowlands DJ; Wang J; Stuart DI; Fry EE; Rao Z A sensor-adaptor mechanism for enterovirus uncoating from structures of EV71 NATURE STRUCTURAL&MOLECULAR BIOLOGY 19 424-429, 2012
DOI:10.1038/nsmb.2255

Walter TS; Ren J; Tuthill TJ; Rowlands DJ; Stuart DI; Fry EE A plate-based high-throughput assay for virus stability and vaccine formulation JOURNAL OF VIROLOGICAL METHODS 185 166-170, 2012
DOI:10.1016/j.jviromet.2012.06.014

Wozniak AL; Griffin S; Rowlands D; Harris M; Yi MK; Lemon SM; Weinman SA Intracellular Proton Conductance of the Hepatitis C Virus p7 Protein and Its Contribution to Infectious Virus Production PLOS PATHOG 6 -, 2010
DOI:10.1371/journal.ppat.1001087

Groppelli E; Tuthill TJ; Rowlands DJ Cell entry of the aphthovirus equine rhinitis A virus is dependent on endosome acidification. J Virol 84 6235-6240, 2010
DOI:10.1128/JVI.02375-09
View abstract

Tuthill TJ; Groppelli E; Hogle JM; Rowlands DJ Picornaviruses. Curr Top Microbiol Immunol 343 43-89, 2010
DOI:10.1007/82_2010_37
View abstract

Fry EE; Tuthill TJ; Harlos K; Walter TS; Rowlands DJ; Stuart DI Crystal structure of equine rhinitis A virus in complex with its sialic acid receptor J GEN VIROL 91 1971-1977, 2010
DOI:10.1099/vir.0.020420-0

Tuthill TJ; Groppelli E; Rowlands DJ; Hogle JM Picornaviruses Current Topics in Microbiology and Immunology 343 43-89, 2010
DOI:10.1007/82-2010-37
View abstract

Adair R; Patel AH; Corless L; Griffin S; Rowlands DJ; McCormick CJ Expression of hepatitis C virus (HCV) structural proteins in trans facilitates encapsidation and transmission of HCV subgenomic RNA. J Gen Virol 90 833-842, 2009
DOI:10.1099/vir.2008.006049-0
View abstract

Goodwin S; Tuthill TJ; Arias A; Killington RA; Rowlands DJ Foot-and-mouth disease virus assembly: processing of recombinant capsid precursor by exogenous protease induces self-assembly of pentamers in vitro in a myristoylation-dependent manner. J Virol 83 11275-11282, 2009
DOI:10.1128/JVI.01263-09
View abstract

StGelais C; Foster TL; Verow M; Atkins E; Fishwick CW; Rowlands D; Harris M; Griffin S Determinants of hepatitis C virus p7 ion channel function and drug sensitivity identified in vitro. J Virol 83 7970-7981, 2009
DOI:10.1128/JVI.00521-09
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Tuthill TJ; Harlos K; Walter TS; Knowles NJ; Groppelli E; Rowlands DJ; Stuart DI; Fry EE Equine rhinitis A virus and its low pH empty particle: clues towards an aphthovirus entry mechanism? PLoS Pathog 5 e1000620-, 2009
DOI:10.1371/journal.ppat.1000620
View abstract

Davis MP; Bottley G; Beales LP; Killington RA; Rowlands DJ; Tuthill TJ Recombinant VP4 of human rhinovirus induces permeability in model membranes J VIROL 82 4169-4174, 2008
DOI:10.1128/JVI.01070-07

Griffin S; Trowbridge R; Thommes P; Parry N; Rowlands D; Harris M; Bright H Chimeric GB virus B genomes containing hepatitis C virus p7 are infectious in vivo. J Hepatol 49 908-915, 2008
DOI:10.1016/j.jhep.2008.07.020
View abstract

Griffin S; Stgelais C; Owsianka AM; Patel AH; Rowlands D; Harris M Genotype-dependent sensitivity of hepatitis C virus to inhibitors of the p7 ion channel. Hepatology 48 1779-1790, 2008
DOI:10.1002/hep.22555
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Bartlett NW; Walton RP; Edwards MR; Aniscenko J; Caramori G; Zhu J; Glanville N; Choy KJ; Jourdan P; Burnet J; Tuthill TJ; Pedrick MS; Hurle MJ; Plumpton C; Sharp NA; Bussell JN; Swallow DM; Schwarze J; Guy B; WAlmond J; Jeffery PK; Lloyd CM; Papi A; Killington RA; Rowlands DJ; Blair ED; Clarke NJ; Johnston SL Mouse models of rhinovirus-induced disease and exacerbation of allergic airway inflammation NAT MED 14 199-204, 2008
DOI:10.1038/nm1713

Rowlands DJ; Skehel JJ Fred Brown: 31 January 1925 - 20 February 2004. Biogr Mem Fellows R Soc 53 93-108, 2007
View abstract

Tuthill TJ; Rowlands DJ; Killington RA Picornavirus entry FUTURE VIROL 2 343-351, 2007
DOI:10.2217/17460794.2.4.343

StGelais C; Tuthill TJ; Clarke DS; Rowlands DJ; Harris M; Griffin S Inhibition of hepatitis C virus p7 membrane channels in a liposome-based assay system ANTIVIR RES 76 48-58, 2007
DOI:10.1016/j.antiviral.2007.05.001

McCormick CJ; Brown D; Griffin S; Challinor L; Rowlands DJ; Harris M A link between translation of the hepatitis C virus polyprotein and polymerase function; possible consequences for hyperphosphorylation of NS5A J GEN VIROL 87 93-102, 2006
DOI:10.1099/vir.0.81180.0

Tuthill TJ; Bubeck D; Rowlands DJ; Hogle JM Characterization of early steps in the poliovirus infection process: Receptor-decorated liposomes induce conversion of the virus to membrane-anchored entry-intermediate particles J VIROL 80 172-180, 2006
DOI:10.1128/JVI.80.1.172-180.2006

Ellingham M; Bunka DHJ; Rowlands DJ; Stonehouse NJ Selection and characterization of RNA aptamers to the RNA-dependent RNA polymerase from foot-and-mouth disease virus RNA 12 1970-1979, 2006
DOI:10.1261/rna.161006

Clarke D; Griffin S; Beales L; Gelais CS; Burgess S; Harris M; Rowlands D Evidence for the formation of a heptameric ion channel complex by the hepatitis C virus p7 protein in vitro J BIOL CHEM 281 37057-37068, 2006
DOI:10.1074/jbc.M602434200

McCormick CJ; Maucourant S; Griffin S; Rowlands DJ; Harris M Tagging of NS5A expressed from a functional hepatitis C virus replicon J GEN VIROL 87 635-640, 2006
DOI:10.1099/vir.0.81553-0

Gilbert RJC; Beales LP; Blond DMR; Simon MN; Lin BY; Chisari FV; Stuart DI; Rowlands DJ Hepatitis B small surface antigen particles are octahedral Proceedings of the National Academy of Sciences of the United States of America 102 14783-14788, 2005
DOI:10.1073/pnas.0505062102
View abstract

Fry EE; Stuart DI; Rowlands DJ The structure of foot-and-mouth disease virus CURR TOP MICROBIOL 288 71-101, 2005

Fry EE; Stuart DI; Rowlands DJ The structure of foot-and-mouth disease virus Current Topics in Microbiology and Immunology 288 71-101, 2005
View abstract

Griffin SDC; Clarke D; McCormick CJ; Rowlands DJ; Harris MPG Signal peptide cleavage and internal targeting signals direct the hepatitis C virus p7 protein to distinct intracellular membranes Journal of Virology 79 15525-15536, 2005
DOI:10.1128/JVI.79.24.15525-15536.2005
View abstract

Mahy BW; Rowlands DJ In memoriam Fred Brown (1925-2004) - Obituary ARCH VIROL 150 199-200, 2005
DOI:10.1007/s00705-004-0442-4

McCormick CJ; Challinor L; Macdonald A; Rowlands DJ; Harris M Introduction of replication-competent hepatitis C virus transcripts using a tetracycline-regulable baculovirus delivery system. J Gen Virol 85 429-439, 2004
View abstract

Rowlands DJ Foot-and-Mouth Disease peptide vaccines In Current Perspectives - Foot-and-Mouth Disease Virus , 2004

Griffin SD; Harvey R; Clarke DS; Barclay WS; Harris M; Rowlands DJ A conserved basic loop in hepatitis C virus p7 protein is required for amantadine-sensitive ion channel activity in mammalian cells but is dispensable for localization to mitochondria. J Gen Virol 85 451-461, 2004
View abstract

Xiao C; Tuthill TJ; Kelly CMB; Challinor LJ; Chipman PR; Killington RA; Rowlands DJ; Craig A; Rossmann MG Discrimination among rhinovirus serotypes for a variant ICAM-1 receptor molecule J VIROL 78 10034-10044, 2004
DOI:10.1128/JVI.78.18.10034-10044.2004

Beales LP; Holzenburg A; Rowlands DJ Viral internal ribosome entry site structures segregate into two distinct morphologies. J Virol 77 6574-6579, 2003
View abstract

Rowlands DJ Special issue - Foot-and-mouth disease - Preface Virus Research 91 1-1, 2003
DOI:10.1016/S0168-1702(02)00264-2

Griffin SDC; Beales LP; Clarke DS; Worsfold O; Evans SD; Jaeger J; Harris MPG; Rowlands DJ The p7 protein of hepatitis C virus forms an ion channel that is blocked by the antiviral drug, Amantadine FEBS LETT 535 34-38, 2003
DOI:10.1016/S0014-5793(02)03851-6

O'Farrell D; Trowbridge R; Rowlands DJ; Jaeger J Substrate complexes of hepatitis C virus RNA polymerase (HC-J4): Structural evidence for nucleotide import and De-novo initiation Journal of Molecular Biology 326 1025-1035, 2003
DOI:10.1016/S0022-2836(02)01439-0

Tuthill TJ; Papadopoulos NG; Jourdan P; Challinor LJ; Sharp NA; Plumpton C; Shah K; Barnard S; Dash L; Burnet J; Killington RA; Rowlands DJ; Clarke NJ; Blair ED; Johnston SL Mouse respiratory epithelial cells support efficient replication of human rhinovirus. J Gen Virol 84 2829-2836, 2003
View abstract

McCormick CJ; Rowlands DJ; Harris M Efficient delivery and regulable expression of hepatitis C virus full-length and minigenome constructs in hepatocyte-derived cell lines using baculovirus vectors J GEN VIROL 83 383-394, 2002

Aoubala M; Holt J; Clegg RA; Rowlands DJ; Harris M The inhibition of cAMP-dependent protein kinase by full-length hepatitis C virus NS3/4A complex is due to ATP hydrolysis. J Gen Virol 82 1637-1646, 2001
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Beales LP; Rowlands DJ; Holzenburg A The internal ribosome entry site (IRES) of hepatitis C virus visualized by electron microscopy RNA 7 661-670, 2001

Sharma Oates A; Rao Z; Fry E; Booth T; Jones EY; Rowlands DJ; Simmons D; Stuart DI Specific interactions between human integrin alpha v beta 3 and chimeric hepatitis B virus core particles bearing the receptor binding epitope of foot and mouth disease virus. Virology 239 150-157, 1997

Honda M; Ping LH; Rijnbrand RCA; Amphlet A; Clarke B; Rowlands DJ; Lemon SM Structural requirements for initiation of translation by internal ribosome entry within genome-length hepatitis C virus RNA. Virology 222 31-42, 1996

Curry S; Fry E; Blakemore W; AbuGhaazaleh R; Jackson T; King A; Lea S; Newman J; Rowlands DJ; Stuart D Perturbations in the surface structure of A22 Iraq foot-and-mouth disease virus accompanying coupled changes in host cell specificity and antigenicity Structure 4 135-145, 1996

Chambers M; Dougan G; Newman J; Brown F; Crowther J; Mould P; Humphries MJ; Francis M; Clarke B; Brown AL; Rowlands DJ Chimeric hepatitis B virus core particles as probes for studying peptide-integrin interactions. Journal of Virology 70 4045-4052, 1996
View abstract

Pegna M; Molinari H; Zetta L; Melacini G; Gibbons WA; Brown F; Rowlands DJ; Chan E; Mascagni P The solution conformational features of two highly homologous antigenic peptides of Foot and Mouth Disease Virus serotype A, variants A and USA, correlate with their serological properties. Journal of Peptide Science 2 91-105, 1996

Reynolds JE; Kaminski A; Carroll AR; Clarke BE; Rowlands DJ; Jackson RJ Internal initiation of translation of hepatitis C virus RNA: The ribosome entry site is at the authentic initiation codon. RNA 2 867-878, 1996

Rowlands DJ Editorship: Virus Research Virus Research 91 -,