Faculty of Biological Sciences

Dr Neil Ranson

BSc, PhD 1997, Bristol
Associate Professor
School of Molecular and Cellular Biology

Background: PhD in Mechanistic Enzymology at the University of Bristol with Professor Tony Clarke, followed by Post-doctoral work cross-training into structural biology with Professor Helen Saibil at Birbkeck College London

Contact:  Astbury 8.108, +44(0) 113 34 37065, email address for  

You can read more about Dr Ranson's interests here:
http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=NAR

Research Interests

Cryo-EM of Macromolecular Assemblies including viruses and amyloids

Faculty Research and Innovation



Studentship information

See also:

Modules managed

BIOL3399 - Extended Research Project Preparation

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC1303 - Introductory Biochemistry: Problem Solving and Data Handling
BIOC2301 - Intermediate Integrated Biochemistry
BIOC2303 - Intermediate Biochemistry: Skills
BIOC3111/12/BIOL3112/MICR3120 a - ATU - Visualising Viruses
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOC3303 - Advanced Biochemistry: Skills
BIOL1112 - The Molecules of Life
BIOL3306 - Biological Sciences Research Project
BIOL5112M/5312M - Bioimaging
BIOL5312M - Bioimaging
BMSC2120 - Scientific Skills

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Dr Neil Ranson  (Associate Professor)

Cryo-EM of Macromolecular Assemblies including viruses and amyloids  

Dr Emma Hesketh  (Research Fellow)

Dr Matthew Iadanza  (Research Fellow)


Postgraduates

Francis Hopkins  
Daniel Hurdiss  
Nicholas Hurst [bs11nh]  
Ethan Morgan  
Brendan Rogers  
Jaskiran Sabharwal [bs12js]  
Rebecca Thompson [RPG]  

Rawson S, Iadanza MG, Ranson NA, Muench SP Erratum: Corrigendum to‘Methods to account for movement and flexibility in cryo-EM data processing’ [Methods (2016) 100 (35–41)] Methods 104 207-, 2016
DOI:10.1016/j.ymeth.2016.05.006
View abstract

Rawson S, Iadanza MG, Ranson NA, Muench SP Methods to account for movement and flexibility in cryo-EM data processing Methods 100 35-41, 2016
DOI:10.1016/j.ymeth.2016.03.011
View abstract

Hurdiss DL, Morgan EL, Thompson RF, Prescott EL, Panou MM, Macdonald A, Ranson NA New Structural Insights into the Genome and Minor Capsid Proteins of BK Polyomavirus using Cryo-Electron Microscopy Structure 24 528-536, 2016
DOI:10.1016/j.str.2016.02.008
View abstract

Huynh NAT, Hesketh EAL, Saxena P, Meshcheriakova Y, Ku YC, Hoang LAT, Johnson JAE, Ranson NAA, Lomonossoff GAP, Reddy VAS Crystal Structure and Proteomics Analysis of Empty Virus-like Particles of Cowpea Mosaic Virus Structure 24 567-575, 2016
DOI:10.1016/j.str.2016.02.011
View abstract

Iadanza MG, Jackson MP, Radford SE, Ranson NA MpUL-multi: Software for Calculation of Amyloid Fibril Mass per Unit Length from TB-TEM Images Scientific Reports 6 -, 2016
DOI:10.1038/srep21078
View abstract

RolfssonÓ, Middleton S, Manfield IW, White SJ, Fan B, Vaughan R, Ranson NA, Dykeman E, Twarock R, Ford J, Cheng Kao C, Stockley PG Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2 Journal of Molecular Biology 428 431-448, 2016
DOI:10.1016/j.jmb.2015.11.014
View abstract

Stockley PG, White SJ, Dykeman E, Manfield I, Rolfsson O, Patel N, Bingham R, Barker A, Wroblewski E, Chandler-Bostock R, Weiß EU, Ranson NA, Tuma R, Twarock R Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid. Bacteriophage 6 e1157666-, 2016
View abstract

Hesketh EL, Meshcheriakova Y, Dent KC, Saxena P, Thompson RF, Cockburn JJ, Lomonossoff GP, Ranson NA Mechanisms of assembly and genome packaging in an RNA virus revealed by high-resolution cryo-EM Nature Communications 6 -, 2015
DOI:10.1038/ncomms10113
View abstract

Thompson RF, Walker M, Siebert CA, Muench SP, Ranson NA An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology Methods -, 2015
DOI:10.1016/j.ymeth.2016.02.017
View abstract

Tipping KW, Karamanos TK, Jakhria T, Iadanza MG, Goodchild SC, Tuma R, Ranson NA, Hewitt EW, Radford SE pH-induced molecular shedding drives the formation of amyloid fibril-derived oligomers Proceedings of the National Academy of Sciences of the United States of America 112 E5691-E5696, 2015
DOI:10.1073/pnas.1423174112
View abstract

Geraets JA, Dykeman EC, Stockley PG, Ranson NA, Twarock R Asymmetric Genome Organization in an RNA Virus Revealed via Graph-Theoretical Analysis of Tomographic Data PLoS Computational Biology 11 -, 2015
DOI:10.1371/journal.pcbi.1004146
View abstract

Patel N, Dykeman EC, Coutts RHA, Lomonossoff GP, Rowlands DJ, Phillips SEV, Ranson N, Twarock R, Tuma R, Stockley PG, Johnson JE Revealing the density of encoded functions in a viral RNA Proceedings of the National Academy of Sciences of the United States of America 112 2227-2232, 2015
DOI:10.1073/pnas.1420812112
View abstract

Bakker SE, Groppelli E, Pearson AR, Stockley PG, Rowlands DJ, Ranson NA Limits of structural plasticity in a picornavirus capsid revealed by a massively expanded equine rhinitis A virus particle. Journal of Virology 88 6093-6099, 2014
DOI:10.1128/JVI.01979-13
View abstract

Goodchild SC, Sheynis T, Thompson R, Tipping KW, Xue WF, Ranson NA, Beales PA, Hewitt EW, Radford SE β2-Microglobulin amyloid fibril-induced membrane disruption is enhanced by endosomal lipids and acidic pH. PLoS One 9 e104492-, 2014
DOI:10.1371/journal.pone.0104492
View abstract

Dent K, Thompson R, Barker A, Hiscox J, Barr J, Stockley P, Ranson N The Asymmetric Structure of an Icosahedral Virus Bound to Its Receptor Suggests a Mechanism for Genome Release Structure 21 1225-1234, 2013
DOI:10.1016/j.str.2013.05.012
View abstract

Young A, Stoilova-Mcphie S, Rothnie A, Vallis Y, Harvey-Smith P, Ranson N, Kent H, Brodsky FM, Pearse BMF, Roseman A, Smith CJ Hsc70-induced changes in clathrin-auxilin cage structure suggest a role for clathrin light chains in cage disassembly Traffic -, 2013
DOI:10.1111/tra.12085
View abstract

Stockley PG, Ranson NA, Twarock R A new paradigm for the roles of the genome in ssRNA viruses FUTURE VIROLOGY 8 531-543, 2013
DOI:10.2217/FVL.12.84

Ariza A, Tanner SJ, Walter CT, Dent KC, Shepherd DA, Wu W, Matthews SV, Hiscox JA, Green TJ, Luo M, Elliott RM, Fooks AR, Ashcroft AE, Stonehouse NJ, Ranson NA, Barr JN, Edwards TA Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization. Nucleic Acids Research 41 5912-5926, 2013
DOI:10.1093/nar/gkt268
View abstract

Ford RJ, Barker AM, Bakker SE, Coutts RH, Ranson NA, Phillips SE, Pearson AR, Stockley PG Sequence-specific, RNA-protein interactions overcome electrostatic barriers preventing assembly of satellite tobacco necrosis virus coat protein. J Mol Biol 425 1050-1064, 2013
DOI:10.1016/j.jmb.2013.01.004
View abstract

Keef T, Wardman JP, Ranson NA, Stockley PG, Twarock R Structural constraints on the three-dimensional geometry of simple viruses: Case studies of a new predictive tool Acta Crystallographica Section A: Foundations of Crystallography 69 140-150, 2013
DOI:10.1107/S0108767312047150
View abstract

Stockley PG, Barker AM, Borodavka A, Ford RJ, Pearson AR, Ranson NA, Tuma R, Twarock R, Dykeman E, Phillips SEV, Bakker SE Packaging signals in single-stranded RNA viruses: nature's alternative to a purely electrostatic assembly mechanism Journal of Biological Physics 1-11, 2013
DOI:10.1007/s10867-013-9313-0
View abstract

Bakker SE, Ford RJ, Barker AM, Robottom J, Saunders K, Pearson AR, Ranson NA, Stockley PG Isolation of an asymmetric RNA uncoating intermediate for a single-stranded RNA plant virus. J Mol Biol 417 65-78, 2012
DOI:10.1016/j.jmb.2012.01.017
View abstract

Dykeman EC, Grayson NE, Toropova K, Ranson NA, Stockley PG, Twarock R Simple Rules for Efficient Assembly Predict the Layout of a Packaged Viral RNA J MOL BIOL 408 399-407, 2011
DOI:10.1016/j.jmb.2011.02.039

Toropova K, Stockley PG, Ranson NA Visualising a Viral RNA Genome Poised for Release from Its Receptor Complex J MOL BIOL 408 408-419, 2011
DOI:10.1016/j.jmb.2011.02.040

Rolfsson O, Toropova K, Ranson NA, Stockley PG Mutually-induced conformational switching of RNA and coat protein underpins efficient assembly of a viral capsid. J Mol Biol 401 309-322, 2010
DOI:10.1016/j.jmb.2010.05.058
View abstract

Rolfsson O, Toropova K, Ranson NA, Stockley PG Mutually-induced Conformational Switching of RNA and Coat Protein Underpins Efficient Assembly of a Viral Capsid J MOL BIOL 401 309-322, 2010
DOI:10.1016/j.jmb.2010.05.058

Carter SD, Dent KC, Atkins E, Foster TL, Verow M, Gorny P, Harris M, Hiscox JA, Ranson NA, Griffin S, Barr JN Direct visualization of the small hydrophobic protein of human respiratory syncytial virus reveals the structural basis for membrane permeability FEBS LETT 584 2786-2790, 2010
DOI:10.1016/j.febslet.2010.05.006

Toropova K, Basnak G, Twarock R, Stockley PG, Ranson NA The three-dimensional structure of genomic RNA in bacteriophage MS2: Implications for assembly J MOL BIOL 375 824-836, 2008
DOI:10.1016/j.jmb.2007.08.067

Rolfsson O, Toropova K, Morton V, Francese S, Basnak G, Thompson GS, Homans SW, Ashcroft AE, Stonehouse NJ, Ranson NA, Stockley PG RNA packing specificity and folding during assembly of the bacteriophage MS2 COMPUT MATH METHOD M 9 339-349, 2008
DOI:10.1080/17486700802168445

Toropova K, Basnak G, Twarock R, Stockley PG, Ranson NA The three-dimensional structure of genomic RNA in bacteriophage MS2: Implications for assembly, 2007
DOI:10.1016/j.jmb.2007.08.067

Ranson NA, Stromer T, Bousset L, Melki R, Serpell LC Insights into the architecture of the Ure2p yeast protein assemblies from helical twisted fibrils Protein Science 15 2481-2487, 2006
DOI:10.1110/ps.062215206
View abstract

Ranson NA, Clare DK, Farr GW, Houldershaw D, Horwich AL, Saibil HR Allosteric signaling of ATP hydrolysis in GroEL-GroES complexes NAT STRUCT MOL BIOL 13 147-152, 2006
DOI:10.1038/nsmb1046

Stockley PG, Ashcroft AE, Francese S, Thompson GS, Ranson NA, Smith AM, Homans SW, Stonehouse NJ Dissecting the fine details of assembly of a T=3 capsid Journal of Theoretical Medicine 6 119-125, 2005
DOI:10.1080/10273660500149869

Saibil HR, Ranson NA The chaperonin folding machine TRENDS BIOCHEM SCI 27 627-632, 2002

Ranson NA, Farr GW, Roseman AM, Gowen B, Fenton WA, Horwich AL, Saibil HR ATP-Bound States of GroEL Captured by Cryo-Electron Microscopy Cell 107 869-879, 2001
DOI:10.1016/S0092-8674(01)00617-1

Roseman AM, Ranson NA, Gowen BT, Fuller SD, Saibil HR Structures of Unliganded and ATP-Bound States of the Escherichia coli Chaperonin GroEL by Cryoelectron Microscopy Journal of Structural Biology 135 115-125, 2001
DOI:10.1006/jsbi.2001.4374
View abstract

Roseman AM, Ranson NA, Gowen B, Fuller SD, Saibil HR The ATP-bound state of the E Coli Chaperonin GroEL studied by Cryo-Electron Microscopy Journal of Structural Biology 135 115-125, 2001
DOI:10.1006/jsbi.2001.4374

Farr GW, Furtak K, Rowland MB, Ranson NA, Saibil HR, Kirchhausen T, Horwich AL Multivalent binding of nonnative substrate proteins by the chaperonin GroEL. Cell 100 561-573, 2000
DOI:10.1016/s0092-8674(00)80692-3
View abstract

Nouwen N, Ranson N, Saibil H, Wolpensinger B, Engel A, Ghazi A, Pugsley AP Secretin PulD: association with pilot PulS, structure, and ion-conducting channel formation. Proceedings of the National Academy of Sciences of the United States of America 96 8173-8177, 1999
DOI:10.1073/pnas.96.14.8173
View abstract

Ranson NA, White HE, Saibil HR Chaperonins. The Biochemical journal 333 ( Pt 2) 233-242, 1998
DOI:10.1042/bj3330233
View abstract

Kad NM, Ranson NA, Cliff MJ, Clarke AR Asymmetry, commitment and inhibition in the GroE ATPase cycle impose alternating functions on the two GroEL rings. Journal of molecular biology 278 267-278, 1998
DOI:10.1006/jmbi.1998.1704
View abstract

Ranson NA, Burston SG, Clarke AR Binding, encapsulation and ejection: substrate dynamics during a chaperonin-assisted folding reaction. Journal of molecular biology 266 656-664, 1997
DOI:10.1006/jmbi.1996.0815
View abstract

Ranson NA, Dunster NJ, Burston SG, Clarke AR Chaperonins can catalyse the reversal of early aggregation steps when a protein misfolds. Journal of molecular biology 250 581-586, 1995
DOI:10.1006/jmbi.1995.0399
View abstract

Burston SG, Ranson NA, Clarke AR The origins and consequences of asymmetry in the chaperonin reaction cycle. Journal of molecular biology 249 138-152, 1995
DOI:10.1006/jmbi.1995.0285
View abstract

Chen S, Roseman AM, Hunter AS, Wood SP, Burston SG, Ranson NA, Clarke AR, Saibil HR Location of a folding protein and shape changes in GroEL-GroES complexes imaged by cryo-electron microscopy. Nature 371 261-264, 1994
DOI:10.1038/371261a0
View abstract