Faculty of Biological Sciences

Dr Alan Radford

BSc, Leeds; MSc, PhD, McMaster.
Emeritus Professor
School of Biology

Background: Research Fellow, Stanford University 1966-70; Assistant Professor, UCLA 1970-71; Lecturer, Birkbeck College 1972-74; Lecturer, University of Leeds 1974-82; Senior Lecturer, University of Leeds 1982-2005; Visiting Professor, University of Brasilia 1987 and 1997; Visiting Professor, Oregon Graduate Institute 2004-07; Visiting Professor, Texas A & M University 2008-2010; C Biol, FRSB.

Contact:  Garstang 10.127b, +44(0) 113 34 33072, email address for  

Research Interests

Genomics of fungi, particularly the ascomycete Neurospora crassa: (a) integration of genetic and physical mapping data; (b) gene annotation; (c) maintenance of a Neurospora crassa genome database; (d) origin and evolution of gene families, particularly those for secreted hydrolytic enzymes; (e) ribosomes and other complex heterooligomeric protein complexes.

Radford A Burning bushes New Scientist 193 pp.57-, 2007

Radford AJ, Rothel JS, Jones SL, Sberna G Tuberculosis diagnostic tests: Sensitivity, specificity, and comparing apples with apples [1] American Journal of Respiratory and Critical Care Medicine 174 953-, 2006

Radford AJ, Rothel JS, Sberna G Whole blood IFN-γ assay for detecting TB in children [3] Thorax 61 919-920, 2006

Radford A, Davis RH John R. S. Fincham (1926-2005): a life in microbial genetics. Genetics. 171 1-5, 2005

Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler S, Bell-Pederson D, Paietta J, Plesofsky N, Plamann M, Goodrich-Tanrikulu M, Schulte U, Mannhaupt G, Nargang F, Radford A, Selitrennikoff CP, Galagan JE, Dunlap J, Loros J, Catcheside D, Inoue HH, Aramayo R, Polymenis M, Selker EU, Sachs MS, Marzluf GA, Paulsen I, Davis RH, Ebbole DJ, Zelter A, O'Rourke R, Bowring F, Yeadon J, Ishii C, Suzuki K, Sakai W, Pratt R Lessons from the genome sequence of Neurospora crassa: Tracing the path from genomic blueprint to multicellular organism Microbiology and Molecular Biology Reviews 68 1-109, 2004
View abstract

Radford A Metabolic highways of Neurospora crassa revisited Advances in Genetics 52 165-207, 2004

Radford A Polysaccharidases In The Mycota III, Biochemistry and Molecular Biology , 2004

Wood PR, Jones SL, Agadi S, Bothamley G, Lalvani A, Ewer K, Bryant G, Monk P, Radford AJ, Rothel JS Diagnosis of tuberculosis [2] (multiple letters) Lancet 361 2081-2083, 2003
DOI:10.1016/S0140-6736(03)13653-7

Radford AJ, Rothel JS Diagnosis of tuberculosis. Lancet 361 2083-, 2003

Rothel JS, Radford AJ Comparison of tuberculosis tests: Finding truth or confirming prejudice? [4] Clinical Infectious Diseases 36 1206-1207, 2003
DOI:10.1086/374670

Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read NO, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CPC, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B The genome sequence of the filamentous fungus Neurospora crassa Nature 422 859-868, 2003
DOI:10.1038/nature01554
View abstract

Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang SG, Nielson CB, Butler J, Endrizzi M, Qui DY, Ianakiev P, Pedersen DB, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stabge-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li WX, Pratt RJ, Osmani SA, DeSouza CPC, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seller S, Dunlap J, Aramayo R, Radford A, Natvig DO, Alex LA, Mannhaupt G, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B, Ebbole DJ The genome sequence of the filamentous fungus Neurospora crassa Nature 422 859-868, 2003
DOI:10.1038/nature01554

Radford A, Sachs A, Perkins DD The Neurospora Compendium: Chromosomal Loci, 2001

Radford A, Jiang M Exploitation of a cellulose-binding domain from Neurospora crassa Enzyme and Microbial Technology 27 434-442, 2000

Moore RJ, Rothel L, Krywult J, Radford AJ, Lund K, Hodgson ALM Foreign gene expression in Corynebacterium pseudotuberculosis: Development of a live vaccine vector Vaccine 18 487-497, 1999
DOI:10.1016/S0264-410X(99)00243-1
View abstract

Radford A Humicola grisea var. thermoidea cbh1.2: A new gene in the family of cellobiohydrolases is expressed and encodes a cellulose-binding domain-less enzyme The Journal of General and Applied Microbioloy 43 115-120, 1997

Radford A The genome and genes of Neurospora crassa Fungal Genetics and Biology 21 258-266, 1997

Bruchez JJP, Eberle J, Kohler W, Kruft V, Radford A, Russo VEA bli-4, a gene that is rapidly induced by blue light, encodes a novel mitochondrial, short chain alcohol dehydrogenase-like protein in Neurospora crassa Molecular and General Genetics 252 223-229, 1996
DOI:10.1007/s004380050223
View abstract

Baumgart KW, Mckenzie KR, Radford AJ, Ramshaw I, Britton WJ Immunogenicity and protection studies with recombinant mycobacteria and vaccinia vectors coexpressing the 18-kilodalton protein of Mycobacterium leprae Infection and Immunity 64 2274-2281, 1996
View abstract

Radford A A fast blue-light regulated gene encoding a novel mitochondrial, short-chain alcohol dehydrogenase-like protein in Neurospora crassa Molecular and General Genetics 252 223-229, 1996

Radford A, Bruchez JJP, Taleb F, Stone PJ Mutation in Neurospora crassa - from X-rays to RIP In Fungal Genetics - Principles and Practice , 1996

Radford A, Stone PJ, Taleb F Cellulase and amylase complexes In The Mycota III , 1996

O'Neill MP, Anne Eady E, Radford A, Baumberg S, Cove JH The use of PCR to isolate a putative ABC transporter from Saccharopolyspora erythraea FEMS Microbiology Letters 131 189-195, 1995
DOI:10.1016/0378-1097(95)00258-7
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Radford A The cellulase complex of Neurospora crassa: cbh-1 cloning, sequencing and homologies Gene 161 137-138., 1995

Billman-Jacobe H, Wang L, Kortt A, Stewart D, Radford A Expression and secretion of heterologous proteases by Corynebacterium glutamicum Applied and Environmental Microbiology 61 1610-1613, 1995
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Hodgson ALM, Tachedjian M, Corner LA, Radford AJ Protection of sheep against caseous lymphadenitis by use of a single oral dose of live recombinant Corynebacterium pseudotuberculosis Infection and Immunity 62 5275-5280, 1994
View abstract

DORAN TJ, DAVIES JK, RADFORD AJ, HODGSON ALM Putative functional domain within ORF2 on the Mycobacterium insertion sequences IS900 and IS902 Immunology and Cell Biology 72 427-434, 1994
DOI:10.1111/j.1440-1711.1994.tb03812.x
View abstract

Radford A Evolution of transcription-regulating proteins by enzyme recruitment: molecular models for nitrogen metabolite repression and ethanol utilization in eukaryotes Gene 146 145-158., 1994

Billman-Jacobe H, Hodgson ALM, Lightowlers M, Wood PR, Radford AJ Expression of ovine gamma interferon in Escherichia coli and Corynebacterium glutamicum Applied and Environmental Microbiology 60 1641-1645, 1994
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Collins DM, Radford AJ, de Lisle GW, Billman-Jacobe H Diagnosis and epidemiology of bovine tuberculosis using molecular biological approaches Veterinary Microbiology 40 83-94, 1994
DOI:10.1016/0378-1135(94)90048-5
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Doran TJ, Hodgson ALM, Davies JK, Radford AJ Characterisation of a highly repeated DNA sequence from Mycobacterium bovis FEMS Microbiology Letters 111 147-152, 1993
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Radford A A fungal phylogeny based upon orotidine 5''-monophosphate decarboxylase J Mol Evol 36 389-395, 1993

Stone PJA, Makoff AJB, Parish JHA, Radford A Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa Current Genetics 24 205-211, 1993
DOI:10.1007/BF00351793
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Jones SL, Cox JC, Shepherd JM, Rothel JS, Wood PR, Radford AJ Removal of false-positive reactions from plasma in an enzyme immunoassay for bovine interferon-γ Journal of Immunological Methods 155 233-240, 1992
DOI:10.1016/0022-1759(92)90290-A
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Doran TJ, Hodgson ALM, Davies JK, Radford AJ Characterisation of a novel repetitive DNA sequence from Mycobacterium bovis FEMS Microbiology Letters 96 179-185, 1992
DOI:10.1016/0378-1097(92)90400-I
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Woodward JR, Radford A, Rodriguez L, Aguado J, Romero MD Immobilization ofβ‐glucosidase from different sources on nylon tube Acta Biotechnologica 12 357-364, 1992
DOI:10.1002/abio.370120502
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Hodgson ALM, Krywult J, Corner LA, Rothel JS, Radford AJ Rational attenuation of Corynebacterium pseudotuberculosis: Potential cheesy gland vaccine and live delivery vehicle Infection and Immunity 60 2900-2905, 1992
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Fifis T, Costopoulos C, Radford AJ, Bacic A, Wood PR Purification and characterization of major antigens from a Mycobacterium bovis culture filtrate Infection and Immunity 59 800-807, 1991
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Radford AJ, Hodgson ALM Construction and characterization of a Mycobacterium-Escherichia coli shuttle vector Plasmid 25 149-153, 1991
DOI:10.1016/0147-619X(91)90029-V
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Radford AJ, Hodgson AL, Rothel JS, Wood PR Cloning and sequencing of the ovine gamma-interferon gene. Australian veterinary journal 68 82-84, 1991
DOI:10.1111/j.1751-0813.1991.tb00755.x
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De Azevedo OM, Felipe MSS, Astolfi-Filho S, Radford A Cloning, sequencing and homologies of the cbh-1 (exoglucanase) gene of Humicola grisea var- thermoidea Journal of General Microbiology 136 2569-2576, 1990
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Seow HF, Rothel JS, Radford AJ, Wood PR The molecular cloning of ovine interleukin 2 gene by the polymerase chain reaction Nucleic Acids Research 18 7175-, 1990
DOI:10.1093/nar/18.23.7175

Oliviera Alzevedo M, Radford A DNA sequence policy Nucleic Acids Research 18 6195-, 1990

Hodgson ALM, Krywult J, Radford A Nucleotide sequence of the erythromycin resistance gene from the Corynebacterium plasmid pNG2 Nucleic Acids Research 18 1891-, 1990
DOI:10.1093/nar/18.7.1891

Radford AJ, Wood PR, Billman-Jacobe H, Geysen HM, Mason TJ, Tribbick G Epitope mapping of the Mycobacterium bovis secretory protein MBP70 using overlapping peptide analysis Journal of General Microbiology 136 265-272, 1990
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de Oliviera Azevedo M, Radford A Sequence of cbh-1 gene of humicola grisea var. thermoidea Nucleic Acids Research 18 668-, 1990
DOI:10.1093/nar/18.3.668

Yazdi MT, Woodward JR, Radford A The cellulase complex of Neurospora crassa: Activity, stability and release Journal of General Microbiology 136 1313-1319, 1990
DOI:10.1099/00221287-136-7-1313
View abstract

Yazdi MT, Radford A, Keen JN, Woodward JR Cellulase production by Neurospora crassa: Purification and characterization of cellulolytic enzymes Enzyme and Microbial Technology 12 120-123, 1990
DOI:10.1016/0141-0229(90)90084-4
View abstract

Yazdi MT, Woodward JR, Radford A Cellulase production by Neurospora crassa: The enzymes of the complex and their regulation Enzyme and Microbial Technology 12 116-119, 1990
DOI:10.1016/0141-0229(90)90083-3
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Messerotti LJ, Radford AJ, Hodgson ALM Nucleotide sequence of the replication region from the Mycobacterium-Escherichia coli shuttle vector pEP2 Gene 96 147-148, 1990
DOI:10.1016/0378-1119(90)90356-V
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Billman Jacobe H, Radford AJ, Rothel JS, Wood PR Mapping of the T and B cell epitopes of the Mycobacterium bovis protein, MPB70 Immunology and Cell Biology 68 359-365, 1990
DOI:10.1111/j.1440-1711.1990.tb03670.x
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Plackett P, Corner LA, Fifis T, Radford AJ, Ripper JL, Naichang C, Yumei L Discrimination between sheep antibodies to Brucella melitensis and to Brucella ovis Veterinary Microbiology 20 339-348, 1989
DOI:10.1016/0378-1135(89)90058-8
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Garsia RJ, Hellqvist L, Booth RJ, Radford AJ, Britton WJ, Astbury L, Trent RJ, Basten A Homology of the 70-kilodalton antigens from Mycobacterium leprae and Mycobacterium bovis with the Mycobacterium tuberculosis 71-kilodalton antigen with the conserved heat shock protein 70 of eucaryyotes Infection and Immunity 57 204-212, 1989
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Wood P, Fifis T, Corner L, Radford A Isolation and characterisation of an immunodominant protein from Mycobacterium bovis. Acta Leprologica 7 Suppl 1 186-187, 1989

Radford AJ, Duffield BJ, Plackett P Cloning of species-specific antigen of Mycobacterium bovis Infection and Immunity 56 921-925, 1988
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Wood PR, Ripper J, Radford AJ, Bundesen PG, Rylatt DB, Cottis LE, John M, Plackett P Production and characterization of monoclonal antibodies specific for Mycobacterium bovis Journal of General Microbiology 134 2599-2604, 1988
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Radford A, Dix NIM Comparison of the orotidine 5'-monophosphate decarboxylase sequences of eight species Genome 30 501-505, 1988
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Glazebrook JA, Mitchell K, Radford A Molecular genetic analysis of the pyr-4 gene of Neurospora crassa MGG Molecular& General Genetics 209 399-402, 1987
DOI:10.1007/BF00329672
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Radford A, Baumberg S A glossary of terms for teaching genetics Journal of Biological Education 21 127-135, 1987
DOI:10.1080/00219266.1987.9654878

Sazci A, Erenler K, Radford A Detection of cellulolytic fungi by using Congo red as an indicator: a comparative study with the dinitrosalicyclic acid reagent method Journal of Applied Bacteriology 61 559-562, 1986
DOI:10.1111/j.1365-2672.1986.tb01729.x
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Newbury SF, Glazebrook JA, Radford A Sequence analysis of the pyr-4 (orotidine 5'-P decarboxylase) gene of Neurospora crassa Gene 43 51-58, 1986
DOI:10.1016/0378-1119(86)90007-7
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Buxton FP, Radford A The transformation of mycelial spheroplasts of Neurospora crassa and the attempted isolation of an autonomous replicator MGG Molecular& General Genetics 196 339-344, 1984
DOI:10.1007/BF00328068
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Buxton FP, Radford A Cloning of the structural gene for orotidine 5′-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli MGG Molecular& General Genetics 190 403-405, 1983
DOI:10.1007/BF00331067
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Radford A, Bird-Stewart JA Letters Journal of Biological Education 17 2-3, 1983
DOI:10.1080/00219266.1983.9654485

Rigby DJ, Radford A Investigation of binding sites in the complex pyrimidine-specific carbamoyl-phosphate synthetase/aspartate carbamoyl transferase enzyme of Neurospora crassa Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 709 154-159, 1982
DOI:10.1016/0167-4838(82)90455-1
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Perkins DD, Radford A, Newmeyer D, Bjorkman M Chromosomal loci of Neurospora crassa Microbiological Reviews 46 426-570, 1982

Radford A, Bird-Stewart JA Teaching genetics in schools Journal of Biological Education 16 177-180, 1982
DOI:10.1080/00219266.1982.9654454
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Buxton FP, Radford A Nitrogen metabolite repression of fluoropyrimidine resistance and pyrimidine uptake in Neurospora crassa MGG Molecular& General Genetics 186 259-262, 1982
DOI:10.1007/BF00331859
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Buxton FP, Radford A Partial characterization of 5-fluoropyrimidine-resistant mutants of Neurospora crassa MGG Molecular& General Genetics 185 132-135, 1982
DOI:10.1007/BF00333802
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Buxton FP, Radford A Isolation and mapping of fluoropyrimidine-resistant mutants of Neurospora crassa MGG Molecular& General Genetics 185 129-131, 1982
DOI:10.1007/BF00333801
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Radford A, Pope S, Sazci A, Fraser MJ, Parish JH Liposome-mediated genetic transformation of Neurospora crassa MGG Molecular& General Genetics 184 567-569, 1981
DOI:10.1007/BF00352544
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Rigby DJ, Radford A The involvement of proteolysis in conformational stability of the carbamoyl-phosphate synthetase/aspartate carbamoyltransferase enzyme of Neurospora crassa BBA - Enzymology 661 315-322, 1981
DOI:10.1016/0005-2744(81)90020-6
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Makoff AJ, Radford A Genetics and biochemistry of carbamoyl phosphate biosynthesis and its utilization in the pyrimidine biosynthetic pathway Microbiological Reviews 42 307-328, 1978

Makoff AJ, Buxton FP, Radford A A possible model for the structure of the Neurospora carbamoyl phosphate synthase-aspartate carbamoyl transferase complex enzyme MGG Molecular& General Genetics 161 297-304, 1978
DOI:10.1007/BF00331004
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Makoff AJ, Radford A Dissociation of an enzyme complex from Neurospora crassa. Evidence for a protease BBA - Enzymology 485 314-329, 1977
DOI:10.1016/0005-2744(77)90167-X
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Makoff AJ, Radford A Glutamine utilization in both the arginine-specific and pyrimidine-specific carbamoyl phosphate synthase enzymes of Neurospora crassa MGG Molecular& General Genetics 149 175-178, 1976
DOI:10.1007/BF00332886
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Makoff AJ, Radford A The location of the feedback-specific region within the Pyrimidine-3 locus of Neurospora crassa MGG Molecular& General Genetics 146 247-251, 1976
DOI:10.1007/BF00701247
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Radford A Restoration of a single enzyme function in bifunctionally defective non-polar pyirmidine-<inf>3</inf>mutants of neurospora Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 15 23-29, 1972
DOI:10.1016/0027-5107(72)90088-7
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Radford A Change in complementation patterns and enzyme activity of pyrimidine-3 mutants of Neurospora crassa by induced reversion Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 12 57-64, 1971
DOI:10.1016/0027-5107(71)90072-8
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Radford A Intragenic mapping of the Neurospora pyrimidine-3 locus by functional deletions MGG Molecular& General Genetics 109 241-245, 1970
DOI:10.1007/BF00267012
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Radford A Pyrimidine-requiring suppressor mutations of Arginine-3 in Neurospora and their bearing on the structure of the Pyrimidine-3 locus MGG Molecular& General Genetics 107 97-106, 1970
DOI:10.1007/BF00333625
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Radford A, Threlkeld SF Genetically mixed perithecia and somatic recombination in a pseudowild type strain of Neurospora crassa. Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie 12 547-552, 1970
DOI:10.1139/g70-073

Radford A Polarised complementation at the pyrimidine-3 locus of Neurospora Molecular and General Genetics MGG 104 288-294, 1969
DOI:10.1007/BF02539292
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Radford A, Threlkeld SF High frequency of 3:1 tetrad ratios for the PDX-1 locus of Neurospora crassa. Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie 11 60-64, 1969
DOI:10.1139/g69-007

Radford A Information from ICR-170-induced mutations on the structure of the pyramidine-3 locus in neurospora Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 8 537-544, 1969
DOI:10.1016/0027-5107(69)90071-2
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Radford A Variability of recombination within the pyridoxine locus of Neurospora. Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie 10 444-447, 1968
DOI:10.1139/g68-059

Radford A High resolution recombination analysis of the pyridoxine-1 locus of Neurospora. Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie 10 893-897, 1968
DOI:10.1139/g68-111

Radford A Heterokaryon complementation among the pyridoxine auxotrophs of Neurospora crassa. Canadian journal of genetics and cytology. Journal canadien de génétique et de cytologie 7 472-477, 1965
DOI:10.1139/g65-061