Faculty of Biological Sciences

Dr Alan Radford

BSc, Leeds; MSc, PhD, McMaster.
Emeritus Professor
School of Biology

Background: Research Fellow, Stanford University 1966-70; Assistant Professor, UCLA 1970-71; Lecturer, Birkbeck College 1972-74; Lecturer, University of Leeds 1974-82; Senior Lecturer, University of Leeds 1982-2005; Visiting Professor, University of Brasilia 1987 and 1997; Visiting Professor, Oregon Graduate Institute 2004-07; Visiting Professor, Texas A & M University 2008-2010; C Biol, FRSB.

Contact:  Garstang 10.127b, +44(0) 113 34 33072, email address for  

Research Interests

Genomics of fungi, particularly the ascomycete Neurospora crassa: (a) integration of genetic and physical mapping data; (b) gene annotation; (c) maintenance of a Neurospora crassa genome database; (d) origin and evolution of gene families, particularly those for secreted hydrolytic enzymes; (e) ribosomes and other complex heterooligomeric protein complexes.

Radford A Burning bushes New Scientist 193 pp.57-, 2007

Radford A, Davis RH John R. S. Fincham (1926-2005): a life in microbial genetics. Genetics. 171 1-5, 2005

Borkovich KA, Alex LA, Yarden O, Freitag M, Turner GE, Read ND, Seiler S, Bell-Pederson D, Paietta J, Plesofsky N, Plamann M, Goodrich-Tanrikulu M, Schulte U, Mannhaupt G, Nargang F, Radford A, Selitrennikoff CP, Galagan JE, Dunlap J, Loros J, Catcheside D, Inoue HH, Aramayo R, Polymenis M, Selker EU, Sachs MS, Marzluf GA, Paulsen I, Davis RH, Ebbole DJ, Zelter A, O'Rourke R, Bowring F, Yeadon J, Ishii C, Suzuki K, Sakai W, Pratt R Lessons from the genome sequence of Neurospora crassa: Tracing the path from genomic blueprint to multicellular organism Microbiology and Molecular Biology Reviews 68 1-109, 2004
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Radford A Metabolic highways of Neurospora crassa revisited Advances in Genetics 52 165-207, 2004

Radford A Polysaccharidases In The Mycota III, Biochemistry and Molecular Biology , 2004

Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read NO, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang S, Nielsen CB, Butler J, Endrizzi M, Qui D, Ianakiev P, Bell-Pedersen D, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stange-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li W, Pratt RJ, Osmani SA, DeSouza CPC, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seiler S, Dunlap J, Radford A, Aramayo R, Natvig DO, Alex LA, Mannhaupt G, Ebbole DJ, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B The genome sequence of the filamentous fungus Neurospora crassa Nature 422 859-868, 2003
DOI:10.1038/nature01554
View abstract

Galagan JE, Calvo SE, Borkovich KA, Selker EU, Read ND, Jaffe D, FitzHugh W, Ma LJ, Smirnov S, Purcell S, Rehman B, Elkins T, Engels R, Wang SG, Nielson CB, Butler J, Endrizzi M, Qui DY, Ianakiev P, Pedersen DB, Nelson MA, Werner-Washburne M, Selitrennikoff CP, Kinsey JA, Braun EL, Zelter A, Schulte U, Kothe GO, Jedd G, Mewes W, Staben C, Marcotte E, Greenberg D, Roy A, Foley K, Naylor J, Stabge-Thomann N, Barrett R, Gnerre S, Kamal M, Kamvysselis M, Mauceli E, Bielke C, Rudd S, Frishman D, Krystofova S, Rasmussen C, Metzenberg RL, Perkins DD, Kroken S, Cogoni C, Macino G, Catcheside D, Li WX, Pratt RJ, Osmani SA, DeSouza CPC, Glass L, Orbach MJ, Berglund JA, Voelker R, Yarden O, Plamann M, Seller S, Dunlap J, Aramayo R, Radford A, Natvig DO, Alex LA, Mannhaupt G, Freitag M, Paulsen I, Sachs MS, Lander ES, Nusbaum C, Birren B, Ebbole DJ The genome sequence of the filamentous fungus Neurospora crassa Nature 422 859-868, 2003
DOI:10.1038/nature01554

Radford A, Sachs A, Perkins DD The Neurospora Compendium: Chromosomal Loci, 2001

Radford A, Jiang M Exploitation of a cellulose-binding domain from Neurospora crassa Enzyme and Microbial Technology 27 434-442, 2000

Radford A Humicola grisea var. thermoidea cbh1.2: A new gene in the family of cellobiohydrolases is expressed and encodes a cellulose-binding domain-less enzyme The Journal of General and Applied Microbioloy 43 115-120, 1997

Radford A The genome and genes of Neurospora crassa Fungal Genetics and Biology 21 258-266, 1997

Bruchez JJP, Eberle J, Kohler W, Kruft V, Radford A, Russo VEA bli-4, a gene that is rapidly induced by blue light, encodes a novel mitochondrial, short chain alcohol dehydrogenase-like protein in Neurospora crassa Molecular and General Genetics 252 223-229, 1996
DOI:10.1007/s004380050223
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Radford A A fast blue-light regulated gene encoding a novel mitochondrial, short-chain alcohol dehydrogenase-like protein in Neurospora crassa Molecular and General Genetics 252 223-229, 1996

Radford A, Bruchez JJP, Taleb F, Stone PJ Mutation in Neurospora crassa - from X-rays to RIP In Fungal Genetics - Principles and Practice , 1996

Radford A, Stone PJ, Taleb F Cellulase and amylase complexes In The Mycota III , 1996

O'Neill MP, Anne Eady E, Radford A, Baumberg S, Cove JH The use of PCR to isolate a putative ABC transporter from Saccharopolyspora erythraea FEMS Microbiology Letters 131 189-195, 1995
DOI:10.1016/0378-1097(95)00258-7
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Radford A The cellulase complex of Neurospora crassa: cbh-1 cloning, sequencing and homologies Gene 161 137-138., 1995

Radford A Evolution of transcription-regulating proteins by enzyme recruitment: molecular models for nitrogen metabolite repression and ethanol utilization in eukaryotes Gene 146 145-158., 1994

Radford A A fungal phylogeny based upon orotidine 5''-monophosphate decarboxylase J Mol Evol 36 389-395, 1993

Stone PJA, Makoff AJB, Parish JHA, Radford A Cloning and sequence analysis of the glucoamylase gene of Neurospora crassa Current Genetics 24 205-211, 1993
DOI:10.1007/BF00351793
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Woodward JR, Radford A, Rodriguez L, Aguado J, Romero MD Immobilization ofβ‐glucosidase from different sources on nylon tube Acta Biotechnologica 12 357-364, 1992
DOI:10.1002/abio.370120502
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De Azevedo OM, Felipe MSS, Astolfi-Filho S, Radford A Cloning, sequencing and homologies of the cbh-1 (exoglucanase) gene of Humicola grisea var- thermoidea Journal of General Microbiology 136 2569-2576, 1990
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de Oliviera Azevedo M, Radford A Sequence of cbh-1 gene of humicola grisea var. thermoidea Nucleic Acids Research 18 668-, 1990
DOI:10.1093/nar/18.3.668

Yazdi MT, Woodward JR, Radford A The cellulase complex of Neurospora crassa: Activity, stability and release Journal of General Microbiology 136 1313-1319, 1990
DOI:10.1099/00221287-136-7-1313
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Yazdi MT, Radford A, Keen JN, Woodward JR Cellulase production by Neurospora crassa: Purification and characterization of cellulolytic enzymes Enzyme and Microbial Technology 12 120-123, 1990
DOI:10.1016/0141-0229(90)90084-4
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Yazdi MT, Woodward JR, Radford A Cellulase production by Neurospora crassa: The enzymes of the complex and their regulation Enzyme and Microbial Technology 12 116-119, 1990
DOI:10.1016/0141-0229(90)90083-3
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Radford A, Dix NIM Comparison of the orotidine 5'-monophosphate decarboxylase sequences of eight species Genome 30 501-505, 1988
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Glazebrook JA, Mitchell K, Radford A Molecular genetic analysis of the pyr-4 gene of Neurospora crassa MGG Molecular& General Genetics 209 399-402, 1987
DOI:10.1007/BF00329672
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Newbury SF, Glazebrook JA, Radford A Sequence analysis of the pyr-4 (orotidine 5'-P decarboxylase) gene of Neurospora crassa Gene 43 51-58, 1986
DOI:10.1016/0378-1119(86)90007-7
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Sazci A, Erenler K, Radford A Detection of cellulolytic fungi by using Congo red as an indicator: a comparative study with the dinitrosalicyclic acid reagent method Journal of Applied Bacteriology 61 559-562, 1986
DOI:10.1111/j.1365-2672.1986.tb01729.x
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Buxton FP, Radford A The transformation of mycelial spheroplasts of Neurospora crassa and the attempted isolation of an autonomous replicator MGG Molecular& General Genetics 196 339-344, 1984
DOI:10.1007/BF00328068
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Buxton FP, Radford A Cloning of the structural gene for orotidine 5′-phosphate carboxylase of Neurospora crassa by expression in Escherichia coli MGG Molecular& General Genetics 190 403-405, 1983
DOI:10.1007/BF00331067
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Rigby DJ, Radford A Investigation of binding sites in the complex pyrimidine-specific carbamoyl-phosphate synthetase/aspartate carbamoyl transferase enzyme of Neurospora crassa Biochimica et Biophysica Acta (BBA)/Protein Structure and Molecular 709 154-159, 1982
DOI:10.1016/0167-4838(82)90455-1
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Perkins DD, Radford A, Newmeyer D, Bjorkman M Chromosomal loci of Neurospora crassa Microbiological Reviews 46 426-570, 1982

Buxton FP, Radford A Nitrogen metabolite repression of fluoropyrimidine resistance and pyrimidine uptake in Neurospora crassa MGG Molecular& General Genetics 186 259-262, 1982
DOI:10.1007/BF00331859
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Buxton FP, Radford A Partial characterization of 5-fluoropyrimidine-resistant mutants of Neurospora crassa MGG Molecular& General Genetics 185 132-135, 1982
DOI:10.1007/BF00333802
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Buxton FP, Radford A Isolation and mapping of fluoropyrimidine-resistant mutants of Neurospora crassa MGG Molecular& General Genetics 185 129-131, 1982
DOI:10.1007/BF00333801
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Radford A, Pope S, Sazci A, Fraser MJ, Parish JH Liposome-mediated genetic transformation of Neurospora crassa MGG Molecular& General Genetics 184 567-569, 1981
DOI:10.1007/BF00352544
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Rigby DJ, Radford A The involvement of proteolysis in conformational stability of the carbamoyl-phosphate synthetase/aspartate carbamoyltransferase enzyme of Neurospora crassa BBA - Enzymology 661 315-322, 1981
DOI:10.1016/0005-2744(81)90020-6
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Makoff AJ, Radford A Genetics and biochemistry of carbamoyl phosphate biosynthesis and its utilization in the pyrimidine biosynthetic pathway Microbiological Reviews 42 307-328, 1978

Makoff AJ, Buxton FP, Radford A A possible model for the structure of the Neurospora carbamoyl phosphate synthase-aspartate carbamoyl transferase complex enzyme MGG Molecular& General Genetics 161 297-304, 1978
DOI:10.1007/BF00331004
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Makoff AJ, Radford A Dissociation of an enzyme complex from Neurospora crassa. Evidence for a protease BBA - Enzymology 485 314-329, 1977
DOI:10.1016/0005-2744(77)90167-X
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Makoff AJ, Radford A Glutamine utilization in both the arginine-specific and pyrimidine-specific carbamoyl phosphate synthase enzymes of Neurospora crassa MGG Molecular& General Genetics 149 175-178, 1976
DOI:10.1007/BF00332886
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Makoff AJ, Radford A The location of the feedback-specific region within the Pyrimidine-3 locus of Neurospora crassa MGG Molecular& General Genetics 146 247-251, 1976
DOI:10.1007/BF00701247
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Radford A Restoration of a single enzyme function in bifunctionally defective non-polar pyirmidine-<inf>3</inf>mutants of neurospora Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 15 23-29, 1972
DOI:10.1016/0027-5107(72)90088-7
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Radford A Change in complementation patterns and enzyme activity of pyrimidine-3 mutants of Neurospora crassa by induced reversion Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 12 57-64, 1971
DOI:10.1016/0027-5107(71)90072-8
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Radford A Intragenic mapping of the Neurospora pyrimidine-3 locus by functional deletions MGG Molecular& General Genetics 109 241-245, 1970
DOI:10.1007/BF00267012
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Radford A, Threlkeld SF Genetically mixed perithecia and somatic recombination in a pseudowild type strain of Neurospora crassa. Canadian Journal of Genetics and Cytology 12 547-552, 1970

Radford A Pyrimidine-requiring suppressor mutations of Arginine-3 in Neurospora and their bearing on the structure of the Pyrimidine-3 locus MGG Molecular& General Genetics 107 97-106, 1970
DOI:10.1007/BF00333625
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Radford A Information from ICR-170-induced mutations on the structure of the pyramidine-3 locus in neurospora Mutation Research - Fundamental and Molecular Mechanisms of Mutagenesis 8 537-544, 1969
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Radford A Polarised complementation at the pyrimidine-3 locus of Neurospora Molecular and General Genetics MGG 104 288-294, 1969
DOI:10.1007/BF02539292
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Radford A, Threlkeld SF High frequency of 3:1 tetrad ratios for the PDX-1 locus of Neurospora crassa. Canadian Journal of Genetics and Cytology 11 60-64, 1969

Radford A High resolution recombination analysis of the pyridoxine-1 locus of Neurospora. Canadian Journal of Genetics and Cytology 10 893-897, 1968

Radford A Variability of recombination within the pyridoxine locus of Neurospora. Canadian Journal of Genetics and Cytology 10 444-447, 1968

Radford A Heterokaryon complementation among the pyridoxine auxotrophs of Neurospora crassa. Canadian Journal of Genetics and Cytology 7 472-477, 1965