Faculty of Biological Sciences

Dr Sreenivasan Ponnambalam

BSc, PhD, Birmingham
Reader in Human Disease Biology
School of Molecular and Cellular Biology

Background: BSc (1984), PhD (1988), Birmingham University. Postdoctoral work at Stanford University, USA (1988-1991) and Cancer Research UK, London (1991-1995). MRC Senior Research Fellow and Principal Investigator at the Wellcome Trust Biocentre & School of Life Sciences, University of Dundee (1995-2000). Lecturer in Molecular Cell Biology, University of Leeds (2000-2005), Senior Lecturer in Molecular Cell Biology (2005-2011), Reader in Human Disease Biology (2011-Present), Head of Endothelial Cell Biology Unit (2006-Present). Co-Director of the Leeds Bioimaging Facility.

Contact:  Garstang 6.44c, +44(0) 113 34 33007, email address for  

You can read more about Dr Ponnambalam's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=VP
www.fbs.leeds.ac.uk/research/ECBU/

Research Interests

Receptor-ligand Complex Regulation of Blood Vessel Formation, Maintenance and Dysfunction

The vascular system is made of different cell types that cooperate to regulate blood vessel formation and vascular physiology. The endothelial cell monolayer that lines all blood vessels plays key roles in diverse phenomena including new blood vessel sprouting (angiogenesis), blood pressure and wound healing. My laboratory is investigating how diverse substances which circulate in the blood and extracellular fluids such as growth factors or lipid particles regulate intracellular signalling and vascular physiology. Understanding such biological phenomena helps us to also devise new therapeutics and diagnostics for vascular disease therapy. Such strategies would be useful for clinical intervention in arterial repair and regeneration after heart attacks or strokes, diagnosing early events in atherosclerosis, and blocking solid tumour growth and metastasis.

 

 

 

The upper panel depicts a schematic of receptor-ligand activation, intracellular signalling, trafficking and proteolysis with changes in cellular outcomes such as cell proliferation, migration and apoptosis. The lower panels depict wide-field deconvolution microscopy images of primary human endothelial cells stained for key vascular marker proteins such as Von Willebrand Factor (VWF) and platelet-endothelial adhesion molecule 1 (PECAM-1). Each image comprises a projected stack of 15-20 optical sections, each of 0.4 microns thickness. Bar, 20 microns.
Areas from which our research benefits from collaborations within Leeds include the Endothelial Cell Biology Unit which comprises the groups of Ponnambalam, Homer-Vanniasinkam and Walker with an ethos of basic science translation into medicine. Cardiovascular research and translational research is further fostered by links to groups within the MultiDisciplinary Cardiovascular Research Centre (MCRC) and Biomedical Health Research Centre. We are carrying out structure-function studies through collaborations with the Astbury Centre for Structural Molecular Biology. We are collaborating with physical sciences experts to discover new therapeutics and ways of investigating biological function with the School of Chemistry and School of Mathematics.

 

 

Current Projects

1) VEGFR-VEGF regulation of endothelial function - Vascular endothelial growth factors (VEGFs) comprise a multigene family encoding many isoforms and variants. The VEGF-A gene products bind receptor tyrosine kinases (VEGFR1 and VEGFR2) on the surface of endothelial cells to activate intracellular signalling, vasodilation (reduced blood pressure), vasculogenesis, angiogenesis and wound healing. A key feature of endothelial cells is their ability to mediate angiogenesis i.e. the sprouting of new blood vessels from pre-existing ones. Understanding how VEGF-A regulates VEGFR-regulated signalling is important for for stimulating pro-angiogenic outcomes (to repair damaged blood vessels after heart attacks) or anti-angiogenic outcomes (to block blood vessel growth during cancer metastasis).
2) Scavenger receptor regulation of vascular function & atherosclerosis - Scavenger receptors are membrane proteins that bind a diverse variety of lipid particles, phospholipids and pathogens. The human lectin-like LOX-1 scavenger receptor binds oxidised low-density lipoprotein (OxLDL) and promotes atherosclerotic plaque initiation and progression. Polymorphisms in the human LOX-1 gene are linked to increased vascular disease including heart attacks.  LOX-1 is expressed on different vascular tissues including the endothelium, vascular smooth muscle and leukocytes (monocytes and macrophages). Understanding how LOX-1 regulates cell function and vascular physiology will enable us to target it for therapy. One idea is that ‘knocking out’ or inhibiting LOX-1 function could be beneficial for preventing atherosclerosis leading to plaque initiation and progression in arteries.
Our work on receptor-ligand regulation of vascular control of health and disease benefits from collaboration with Leeds basic scientists and clinicians. Our clinical collaborators include cardiologists Dr Stephen Wheatcroft, Professor Mark Kearney (Division of Cardiovascular Research & Diabetes, Faculty of Medicine & Health) and vascular surgeon Professor Shervanthi Homer-Vanniasinkam (Leeds Vascular Institute, Leeds General Infirmary). We have collaborations with Professor Carmen Molina-Paris, Dr Grant Lythe (School of Mathematics), Professor Colin Fishwick and Dr Bruce Turnbull (School of Chemistry). Within the Faculty of Biological Sciences, we have ongoing collaborations with Dr Darren Tomlinson, Dr John Walker, Dr Anastasia Zhuravleva (School of Molecular & Cellular Biology), Dr Michael Harrison, Dr Stephen Muench and Prof. Paul Millner (School of Biomedical Sciences).
Our current work is funded by the British Heart Foundation, Heart Research UK, The Leverhulme Trust, AstraZeneca & Brunei Darussalam government. Previous funding agencies for projects and equipment were from the Medical Research Council, The Wellcome Trust, BBSRC, The Circulation Foundation, European Union, Yorkshire Cancer Research, Pfizer Global Inc., White Rose Network and Yorkshire Enterprise Fellowship scheme.
 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Expression and localisation of human protein kinases
  • Expression and localisation of human scavenger receptors
  • Heart disease, lipid metabolism and therapeutics
  • Receptor tyrosine kinases: targets for therapy in human disease

Postgraduate studentship areas:

  • Vascular endothelial growth factor receptor (VEGFR)-mediated control of cell function
  • Scavenger receptor-mediated regulation of vascular function and atherosclerosis

See also:

Modules managed

BIOL3305 - Advanced Skills in the Biosciences

Modules taught

BIOC2302 - Intermediate Biochemistry: Practicals
BIOC2303 - Intermediate Biochemistry: Skills
BIOC2303/BIOL2303 - Intermediate MCB Skills
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOC3221/22/BIOL3210 A - ATU - Signal trasduction in cancer and cardiovascular disease
BIOL1302 - Introductory Skills for Biological Sciences
BIOL2210/BIOC2301 - Integrated Biochemistry/Biological Membranes
BIOL2211 - Human Diseases
BIOL2301 - Intermediate Skills for Biological Sciences
BIOL2301/03 - Skills for Biol Sci & Biosciences
BIOL2301/03/MICR2320/BIOL3400 - BIOL/BIOC/MICR advanced skills/Skills in the Cell Biology of Human Disease
BIOL3305 - Advanced Skills in the Biosciences
BIOL3305/MICR3325/BIOL3400 - BIOL/MICR advanced skills/Skills in the Cell Biology of Human Disease
BIOL3306 - Biological Sciences Research Project
BIOL3398 - Research Tools and Applications
BIOL3399 - Extended Research Project Preparation
BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5373M - Protein Engineering Laboratory Project
BIOL5392M - Bioscience MSc Research Project
BMSC1103 - Basic Laboratory and Scientific Skills
MICR2120/BIOC2301 - Integrated Biochemistry/Medical Bacteriology
MICR3110 - Medical Microbiology Research Project

Committees

Member of Graduate School Committee (Admissions Tutor (International))

Centre memberships:

Group Leader Dr Sreenivasan Ponnambalam  (Reader in Human Disease Biology)

Receptor-ligand Complex Regulation of Blood Vessel Formation, Maintenance and Dysfunction 

Postgraduates

Izma Abdul Zani (Primary supervisor) 80% FTE
Gary Cuthbert (Primary supervisor) 50% FTE
Jonathan de Siqueira (Primary supervisor) 34% FTE
Joanna Mitchell (Primary supervisor) 50% FTE
Faheem Shaik (Primary supervisor) 35% FTE

Fearnley GW, Odell AF, Latham AM, Mughal NA, Bruns AF, Burgoyne NJ, Homer-Vanniasinkam S, Zachary IC, Hollstein MC, Wheatcroft SB, Ponnambalam S VEGF-A isoforms differentially regulate ATF-2-dependent VCAM-1 gene expression and endothelial-leukocyte interactions Molecular Biology of the Cell 25 2509-2521, 2014
DOI:10.1091/mbc.E14-05-0962
View abstract

Cubbon RM, Yuldasheva NY, Viswambharan H, Mercer BN, Baliga V, Stephen SL, Askham J, Sukumar P, Skromna A, Mughal RS, Walker AMN, Bruns A, Bailey MA, Galloway S, Imrie H, Gage MC, Rakobowchuk M, Li J, Porter KE, Ponnambalam S, Wheatcroft SB, Beech DJ, Kearney MT Restoring Akt1 activity in outgrowth endothelial cells from South Asian men rescues vascular reparative potential Stem Cells 32 2714-2723, 2014
DOI:10.1002/stem.1766
View abstract

Fearnley GW, Smith GA, Odell AF, Latham AM, Wheatcroft SB, Harrison MA, Tomlinson DC, Ponnambalam S Vascular endothelial growth factor A-stimulated signaling from endosomes in primary endothelial cells Methods in Enzymology 535 265-292, 2014
DOI:10.1016/B978-0-12-397925-4.00016-X
View abstract

Bruns AF, Yuldasheva N, Latham AM, Bao L, Pellet-Many C, Frankel P, Stephen SL, Howell GJ, Wheatcroft SB, Kearney MT, Zachary IC, Ponnambalam S A heat-shock protein axis regulates VEGFR2 proteolysis, blood vessel development and repair. PLoS One 7 e48539-, 2012
DOI:10.1371/journal.pone.0048539
View abstract

Bruns AF, Herbert SP, Odell AF, Jopling HM, Hooper NM, Zachary IC, Walker JH, Ponnambalam S Ligand-Stimulated VEGFR2 Signaling is Regulated by Co-Ordinated Trafficking and Proteolysis TRAFFIC 11 161-174, 2010
DOI:10.1111/j.1600-0854.2009.01001.x

Jopling HM, Odell AF, Hooper NM, Zachary IC, Walker JH, Ponnambalam S Rab GTPase Regulation of VEGFR2 Trafficking and Signaling in Endothelial Cells ARTERIOSCL THROM VAS 29 1119-U206, 2009
DOI:10.1161/ATVBAHA.109.186239

Herbert SP, Odell AF, Ponnambalam S, Walker JH Activation of Cytosolic Phospholipase A(2)-alpha as a Novel Mechanism Regulating Endothelial Cell Cycle Progression and Angiogenesis J BIOL CHEM 284 5784-5796, 2009
DOI:10.1074/jbc.M807282200

Murphy JE, Vohra RS, Dunn S, Holloway ZG, Monaco AP, Homer-Vanniasinkam S, Walker JH, Ponnambalam S Oxidised LDL internalisation by the LOX-1 scavenger receptor is dependent on a novel cytoplasmic motif and is regulated by dynamin-2 J CELL SCI 121 2136-2147, 2008
DOI:10.1242/jcs.020917

De Siqueira J, Zani IA, Russell D, Wheatcroft S, Tomlinson D, Homer-Vanniasinkam S, Ponnambalam S LOX-1-SPECIFIC AFFIMERS BLOCK OXLDL ACCUMULATION IN VITRO, 2017
DOI:10.1136/heartjnl-2017-311726.162

King RJ, Schuett K, Tiede C, Jankowski V, John V, Simmons K, Ponnambalam S, Fishwick C, McPherson M, Tomlinson D, Ajjan R Complement C3 and hypofibrinolysis in diabetes: role of protein glycation and development of a new methodology to reduce thrombosis risk, 2016

Fearnley GW, Smith GA, Abdul-Zani I, Yuldasheva N, Mughal NA, Homer-Vanniasinkam S, Kearney MT, Zachary IC, Tomlinson DC, Harrison MA, Wheatcroft SB, Ponnambalam S VEGF-A isoforms program differential VEGFR2 signal transduction, trafficking and proteolysis Biology Open 5 571-583, 2016
DOI:10.1242/bio.017434
View abstract

Smith GA, Howell GJ, Phillips C, Muench SP, Ponnambalam S, Harrison MA Extracellular and luminal pH regulation by vacuolar H<sup>+</sup>-ATPase isoform expression and targeting to the plasma membrane and endosomes Journal of Biological Chemistry 291 8500-8515, 2016
DOI:10.1074/jbc.M116.723395
View abstract

Windheim M, Höning S, Leppard KN, Butler L, Seed C, Ponnambalam S, Burgert HG Sorting motifs in the cytoplasmic tail of the immunomodulatory E3/49K protein of species D adenoviruses modulate cell surface expression and ectodomain shedding. Journal of Biological Chemistry 291 6796-6812, 2016
DOI:10.1074/jbc.M115.684787
View abstract

Smith GA, Tomlinson DC, Harrison MA, Ponnambalam S Ubiquitin-Mediated Regulation of Cellular Responses to Vascular Endothelial Growth Factors Progress in Molecular Biology and Translational Science -, 2016
DOI:10.1016/bs.pmbts.2016.02.006
View abstract

Peng H, Hao Y, Mousawi F, Roger S, Li J, Sim JA, Ponnambalam S, Yang X, Jiang LH Purinergic and Store-Operated Ca<sup>2+</sup>Signaling Mechanisms in Mesenchymal Stem Cells and their Roles in ATP-Induced Stimulation of Cell Migration Stem Cells -, 2016
DOI:10.1002/stem.2370
View abstract

Smith GA, Fearnley GW, Abdul-Zani I, Wheatcroft SB, Tomlinson DC, Harrison MA, Ponnambalam S VEGFR2 Trafficking, Signaling and Proteolysis is Regulated by the Ubiquitin Isopeptidase USP8 Traffic 17 53-65, 2016
DOI:10.1111/tra.12341
View abstract

De Siqueira J, Abdul Zani I, Russell DA, Wheatcroft SB, Ponnambalam S, Homer-Vanniasinkam S Clinical and Preclinical Use of LOX-1-Specific Antibodies in Diagnostics and Therapeutics Journal of Cardiovascular Translational Research 8 458-465, 2015
DOI:10.1007/s12265-015-9655-z
View abstract

Smith GA, Fearnley GW, Tomlinson DC, Harrison MA, Ponnambalam S The cellular response to vascular endothelial growth factors requires co-ordinated signal transduction, trafficking and proteolysis Bioscience Reports 35 -, 2015
DOI:10.1042/BSR20150171
View abstract

Fearnley GW, Wheatcroft SB, Ponnambalam S Detection and Quantification of Vascular Endothelial Growth Factor Receptor Tyrosine Kinases in Primary Human Endothelial Cells Methods in Molecular Biology 1332 49-65, 2015
DOI:10.1007/978-1-4939-2917-7_4
View abstract

Latham AM, Kankanala J, Fishwick CWG, Ponnambalam S Identification of Receptor Tyrosine Kinase Inhibitors Using Cell Surface Biotinylation and Affinity Isolation Methods in Molecular Biology 1332 121-131, 2015
DOI:10.1007/978-1-4939-2917-7_8
View abstract

Smith GA, Fearnley GW, Harrison MA, Tomlinson DC, Wheatcroft SB, Ponnambalam S Vascular endothelial growth factors: multitasking functionality in metabolism, health and disease Journal of Inherited Metabolic Disease 38 753-763, 2015
DOI:10.1007/s10545-015-9838-4
View abstract

Fearnley GW, Bruns AF, Wheatcroft SB, Ponnambalam S VEGF-A isoform-specific regulation of calcium ion flux, transcriptional activation and endothelial cell migration. Biology open 4 731-742, 2015
DOI:10.1242/bio.201410884
View abstract

Zani IA, Stephen SL, Mughal NA, Russell D, Homer-Vanniasinkam S, Wheatcroft SB, Ponnambalam S Scavenger receptor structure and function in health and disease. Cells 4 178-201, 2015
DOI:10.3390/cells4020178
View abstract

Yang Z, Kirton HM, MacDougall DA, Boyle JP, Deuchars J, Frater B, Ponnambalam S, Hardy ME, White E, Calaghan SC, Peers C, Steele DS The Golgi apparatus is a functionally distinct Ca<sup>2+</sup>store regulated by the PKA and Epac branches of the b1-adrenergic signaling pathway Science Signaling 8 -, 2015
DOI:10.1126/scisignal.aaa7677
View abstract

Karpov OA, Fearnley GW, Smith GA, Kankanala J, McPherson MJ, Tomlinson DC, Harrison MA, Ponnambalam S Receptor tyrosine kinase structure and function in health and disease AIMS Biophysics 2 476-502, 2015
DOI:10.3934/biophy.2015.4.476
View abstract

Latham AM, Kankanala J, Fearnley GW, Gage MC, Kearney MT, Homer-Vanniasinkam S, Wheatcroft SB, Fishwick CWG, Ponnambalam S In silico design and biological evaluation of a dual specificity kinase inhibitor targeting cell cycle progression and angiogenesis PLoS ONE 9 -, 2014
DOI:10.1371/journal.pone.0110997
View abstract

Shaw DJ, Seese R, Ponnambalam S, Ajjan R The role of lectin-like oxidised low-density lipoprotein receptor-1 in vascular pathology Diabetes and Vascular Disease Research 11 410-418, 2014
DOI:10.1177/1479164114547704
View abstract

Jopling HM, Odell AF, Pellet-Many C, Latham AM, Frankel P, Sivaprasadarao A, Walker JH, Zachary IC, Ponnambalam S Endosome-to-plasma membrane recycling of VEGFR2 receptor tyrosine kinase regulates endothelial function and blood vessel formation. Cells 3 363-385, 2014
DOI:10.3390/cells3020363
View abstract

Odell AF, Odell LR, Askham JM, Alogheli H, Ponnambalam S, Hollstein M A Novel p53 Mutant Found in Iatrogenic Urothelial Cancers Is Dysfunctional and Can Be Rescued by a Second-site Global Suppressor Mutation JOURNAL OF BIOLOGICAL CHEMISTRY 288 16704-16714, 2013
DOI:10.1074/jbc.M112.443168

Holloway ZG, Velayos-Baeza A, Howell GJ, Levecque C, Ponnambalam S, Sztul E, Monaco AP Trafficking of the Menkes copper transporter ATP7A is regulated by clathrin-, AP-2-, AP-1-, and Rab22-dependent steps Molecular Biology of the Cell 24 1735-1748, 2013
DOI:10.1091/mbc.E12-08-0625
View abstract

Fearnley GW, Smith GA, Harrison MA, Wheatcroft SB, Tomlinson DC, Ponnambalam S The biochemistry of new blood vessel formation in health and disease OA Biochemistry 1 -, 2013

Bao L, Odell AF, Stephen SL, Wheatcroft SB, Walker JH, Ponnambalam S The S100A6 calcium-binding protein regulates endothelial cell-cycle progression and senescence FEBS Journal 279 4576-4588, 2012
DOI:10.1111/febs.12044
View abstract

Bao L, Odell AF, Stephen SL, Wheatcroft SB, Walker JH, Ponnambalam S The S100A6 calcium-binding protein regulates endothelial cell-cycle progression and senescence. FEBS J 279 4576-4588, 2012
DOI:10.1111/febs.12044
View abstract

Latham AM, Odell AF, Mughal NA, Issitt T, Ulyatt C, Walker JH, Homer-Vanniasinkam S, Ponnambalam S A biphasic endothelial stress-survival mechanism regulates the cellular response to vascular endothelial growth factor A. Exp Cell Res 318 2297-2311, 2012
DOI:10.1016/j.yexcr.2012.06.023
View abstract

Billah MM, Hays HCW, Hodges CS, Ponnambalam S, Vohra R, Millner PA Mixed self-assembled monolayer (mSAM) based impedimetric immunosensors for cardiac troponin i (cTnI) and soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) Sensors and Actuators, B: Chemical 173 361-366, 2012
DOI:10.1016/j.snb.2012.07.017
View abstract

Stephen SL, Ponnambalam S Scavenger receptors: structure, function and diversity Wiley online library -, 2012
DOI:10.1002/9780470015902.a0024247/full

Kankanala J, Latham AM, Johnson AP, Homer-Vanniasinkam S, Fishwick CWG, Ponnambalam S A combinatorial in silico and cellular approach to identify a new class of compounds that target VEGFR2 receptor tyrosine kinase activity and angiogenesis. Br J Pharmacol 166 737-748, 2012
DOI:10.1111/j.1476-5381.2011.01801.x
View abstract

Odell AF, Hollstein M, Ponnambalam S, Walker JH A VE-cadherin-PAR3-α-catenin complex regulates the Golgi localization and activity of cytosolic phospholipase A(2)α in endothelial cells. Mol Biol Cell 23 1783-1796, 2012
DOI:10.1091/mbc.E11-08-0694
View abstract

Twigg MW, Freestone K, Homer-Vanniasinkam S, Ponnambalam S The LOX-1 scavenger receptor and its implications in the treatment of vascular disease Cardiology Research and Practice 1 -, 2012
DOI:10.1155/2012/632408
View abstract

Twigg MW, Freestone K, Homer-Vanniasinkam S, Ponnambalam S The LOX-1 Scavenger Receptor and Its Implications in the Treatment of Vascular Disease. Cardiol Res Pract 2012 632408-, 2012
DOI:10.1155/2012/632408
View abstract

Latham AM, Bruns AF, Kankanala J, Johnson AP, Fishwick CWG, Homer-Vanniasinkam S, Ponnambalam S Indolinones and anilinophthalazines differentially target VEGF-A- and basic fibroblast growth factor-mediated responses in primary human endothelial cells. Br J Pharmacol 165 245-259, 2012
DOI:10.1111/j.1476-5381.2011.01545.x
View abstract

Mughal NA, Russell DA, Ponnambalam S, Homer-Vanniasinkam S Gene therapy in the treatment of peripheral arterial disease. Br J Surg 99 6-15, 2012
DOI:10.1002/bjs.7743
View abstract

Ponnambalam S Different sorts for different sprouts BLOOD 118 490-491, 2011
DOI:10.1182/blood-2011-05-356592

Jopling HM, Howell GJ, Gamper N, Ponnambalam S The VEGFR2 receptor tyrosine kinase undergoes constitutive endosome-to-plasma membrane recycling BIOCHEM BIOPH RES CO 410 170-176, 2011
DOI:10.1016/j.bbrc.2011.04.093

Ponnambalam S, Alberghina M Evolution of the VEGF-Regulated Vascular Network from a Neural Guidance System MOL NEUROBIOL 43 192-206, 2011
DOI:10.1007/s12035-011-8167-3

Mahlin D, Ponnambalam S, Höckerfelt MH, Bergström CA Toward in silico prediction of glass-forming ability from molecular structure alone: a screening tool in early drug development. Molecular pharmaceutics 8 498-506, 2011
DOI:10.1021/mp100339c
View abstract

Ulyatt C, Walker J, Ponnambalam S Hypoxia differentially regulates VEGFR1 and VEGFR2 levels and alters intracellular signaling and cell migration in endothelial cells BIOCHEM BIOPH RES CO 404 774-779, 2011
DOI:10.1016/j.bbrc.2010.12.057

Stephen SL, Freestone K, Dunn S, Twigg MW, Homer-Vanniasinkam S, Walker JH, Wheatcroft SB, Ponnambalam S Scavenger receptors and their potential as therapeutic targets in the treatment of cardiovascular disease. Int J Hypertens 2010 646929-, 2010
DOI:10.4061/2010/646929
View abstract

Latham AM, Molina-Paris C, Homer-Vanniasinkam S, Ponnambalam S An integrative model for vascular endothelial growth factor A as a tumour biomarker INTEGR BIOL-UK 2 397-407, 2010
DOI:10.1039/c0ib00008f

Bruns AF, Bao L, Walker JH, Ponnambalam S VEGF-A-stimulated signalling in endothelial cells via a dual receptor tyrosine kinase system is dependent on co-ordinated trafficking and proteolysis BIOCHEM SOC T 37 1193-1197, 2009
DOI:10.1042/BST0371193

Thomas JC, Vohra RS, Beer S, Bhatti K, Ponnambalam S, Homer-Vanniasinkam S Biomarkers in Peripheral Arterial Disease TRENDS CARDIOVAS MED 19 147-151, 2009

Vohra RS, Walker JH, Howell GJ, Homer-Vanniasinkam S, Ponnambalam S The LOX-1 scavenger receptor cytoplasmic domain contains a transplantable endocytic motif BIOCHEM BIOPH RES CO 383 269-274, 2009
DOI:10.1016/j.bbrc.2009.04.012

Bao LY, Redondo C, Findlay JBC, Walker JH, Ponnambalam S Deciphering soluble and membrane protein function using yeast systems MOL MEMBR BIOL 26 127-135, 2009
DOI:10.1080/09687680802637652

Mittar S, Ulyatt C, Howell GJ, Bruns AF, Zachary I, Walker JH, Ponnambalam S VEGFR1 receptor tyrosine kinase localization to the Golgi apparatus is calcium-dependent EXP CELL RES 315 877-889, 2009
DOI:10.1016/j.yexcr.2008.12.020

Ponnambalam S The molecular&cellular basis of angiogenesis, 2009

Dunn S, Vohra RS, Murphy JE, Homer-Vanniasinkam S, Walker JH, Ponnambalam S The lectin-like oxidized low-density-lipoprotein receptor: a pro-inflammatory factor in vascular disease BIOCHEM J 409 349-355, 2008
DOI:10.1042/BJ20071196

Murphy JE, Vohra RS, Dunn S, Holloway Z, Monaco AP, Homer-Vanniasinkam S, Walker JH, Ponnambalam S Oxidized low-density lipoprotein internalization by the LOX-1 scavenger receptors is dependent on a novel cytoplasmic motif and regulated by dynamin-2 J. Cell Science 121: 2136-2147 -, 2008

Bhatti K, Thomas JC, Homer-Vanniasinkam S, Ponnambalam S Scavenger receptors: therapeutic targets and vascular disease biomarkers? International Atherosclerosis Society -, 2008

Dunn S, Vohra RS, Murphy JE, Homer-Vanniasinkam S, Walker JH, Ponnambalam S The lectin-like oxidized lipoprotein particle scavenger receptor: a pro-inflammatory factory in vascular disease. Biochemical Journal 409 349-355, 2008

Herbert SP, Odell AF, Ponnambalam S, Walker JH The confluence-dependent interaction of cytosolic phospholipase A(2)-alpha with annexin A1 regulates endothelial cell prostaglandin E-2 generation J BIOL CHEM 282 34468-34478, 2007
DOI:10.1074/jbc.M701541200

Herbert SP, Odell AF, Ponnambalam S, Walker JH The confluence-dependent interaction of cytosolic phospholipase A2-alpha with annexin A1 regulates endothelial cell prostaglandin E2 generation., 2007
DOI:10.1074/jbc.M701541200
View abstract

Herbert SP, Odell AF, Ponnambalam S, Walker JH The confluence-dependent interaction of cytosolic phospholipase A<inf>2</inf>-α with annexin A1 regulates endothelial cell prostaglandin E<inf>2</inf>generation Journal of Biological Chemistry 282 34469-34478, 2007
DOI:10.1074/jbc.M701541200
View abstract

Vohra RS, Murphy JE, Walker JH, Homer-Vanniasinkam S, Ponnambalam S Functional refolding of a recombinant C-type lectin-like domain containing intramolecular disulfide bonds PROTEIN EXPRES PURIF 52 415-421, 2007
DOI:10.1016/j.pep.2006.11.012

Netherton CL, McCrossan MC, Denyer M, Ponnambalam S, Armstrong J, Takamatsu HH, Wileman TE African swine fever virus causes microtubule-dependent dispersal of the trans-golgi network and slows delivery of membrane protein to the plasma membrane J VIROL 80 11385-11392, 2006
DOI:10.1128/JVI.00439-06

Mankouri J, Taneja TK, Smith AJ, Ponnambalam S, Sivaprasadarao A Kir6.2 mutations causing neonatal diabetes prevent endocytosis of ATP-sensitive potassium channels EMBO J 25 4142-4151, 2006
DOI:10.1038/sj.emboj.7601275

Ewan LC, Jopling HM, Jia HY, Mittar S, Bagherzadeh A, Howell GJ, Walker JH, Zachary IC, Ponnambalam S Intrinsic tyrosine kinase activity is required for vascular endothelial growth factor receptor 2 ubiquitination, sorting and degradation in endothelial cells TRAFFIC 7 1270-1282, 2006
DOI:10.1111/j.1600-0854.2006.00462.x

Vohra RS, Murphy JE, Walker JH, Ponnambalam S, Homer-Vanniasinkam S Atherosclerosis and the lectin-like OXidized low-density lipoprotein scavenger receptor TRENDS CARDIOVAS MED 16 60-64, 2006

Howell GJ, Holloway ZG, Cobbold C, Monaco AP, Ponnambalam S Cell biology of membrane trafficking in human disease INT REV CYTOL 252 1-69, 2006
DOI:10.1016/S0074-7696(06)52005-4

Murphy JE, Tacon D, Tedbury PR, Hadden JM, Knowling S, Sawamura T, Peckham M, Phillips SEV, Walker JH, Ponnambalam S LOX-1 scavenger receptor mediates calcium-dependent recognition of phosphatidylserine and apoptotic cells BIOCHEM J 393 107-115, 2006

Murphy JE, Tedbury PR, Homer-Vanniasinkam S, Walker JH, Ponnambalam S Biochemistry and cell biology of mammalian scavenger receptors ATHEROSCLEROSIS 182 1-15, 2005
DOI:10.1016/j.atherosclerosis.2005.03.036

Herbert SP, Ponnambalam S, Walker JH Cytosolic phospholipase A(2)-&alpha; mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus Molecular Biology of the Cell 16 3800-3809, 2005
DOI:10.1091/mbc.E05-02-0164
View abstract

Herbert SP, Ponnambalam S, Walker JH Cytosolic phospholipase A(2)-alpha mediates endothelial cell proliferation and is inactivated by association with the Golgi apparatus MOL BIOL CELL 16 3800-3809, 2005
DOI:10.1091/mbc.E05-02-0164

Grewal S, Herbert SP, Ponnambalam S, Walker JH Cytosolic phospholipase A<sub>2</sub>-&alpha; and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus FEBS Journal 272 1278-1290, 2005
DOI:10.1111/j.1742-4658.2005.04565.x
View abstract

Grewal S, Herbert SP, Ponnambalam S, Walker JH Cytosolic phospholipase A2-alpha and cyclooxygenase-2 localize to intracellular membranes of EA.hy.926 endothelial cells that are distinct from the endoplasmic reticulum and the Golgi apparatus. FEBS J 272 1278-1290, 2005
DOI:10.1111/j.1742-4658.2005.04565.x
View abstract

Howell GJ, Herbert SP, Smith JM, Mittar S, Ewan LC, Mohammed M, Hunter AR, Simpson N, Turner AJ, Zachary I, Walker JH, Ponnambalam S Endothelial cell confluence regulates Weibel-Palade body formation MOL MEMBR BIOL 21 413-421, 2004
DOI:10.1080/09687860400011571

Cobbold C, Coventry J, Ponnambalam S, Monaco AP Actin and microtubule regulation of Trans-Golgi network architecture, and copper-dependent protein transport to the cell surface MOL MEMBR BIOL 21 59-66, 2004
DOI:10.1080/096870310001607350

Grewal S, Smith J, Ponnambalam S, Walker J Stimulation-dependent recruitment of cytosolic phospholipase A2-alpha to EA.hy.926 endothelial cell membranes leads to calcium-independent association. Eur J Biochem 271 69-77, 2004
View abstract

Cobbold C, Monaco AP, Sivaprasadarao A, Ponnambalam S Aberrant trafficking of transmembrane proteins in human disease TRENDS CELL BIOL 13 639-647, 2003
DOI:10.1016/j.tcb.2003.10.008

O'Leary R, Ponnambalam S, Wood EJ Pioglitazone-induced myofibroblast cell death: implications for cutaneous scarring BRIT J DERMATOL 149 665-667, 2003

Cobbold C, Coventry J, Ponnambalam S, Monaco AP The Menkes disease ATPase (ATP7A) is internalized via a Rac1-regulated, clathrin- and caveolae-independent pathway HUM MOL GENET 12 1523-1533, 2003
DOI:10.1093/hmg/ddg166

Grewal S, Ponnambalam S, Walker JH Association of cPLA(2)-alpha and COX-1 with the Golgi apparatus of A549 human lung epithelial cells J CELL SCI 116 2303-2310, 2003
DOI:10.1242/jcs.00446

Ponnambalam S Foreword: Protein secretion and the Golgi apparatus MOL MEMBR BIOL 20 97-98, 2003
DOI:10.1080/096878031000104935

Ponnambalam S, Baldwin SA Constitutive protein secretion from the trans-Golgi network to the plasma membrane. Mol Membr Biol 20 129-139, 2003
DOI:10.1080/0968768031000084172
View abstract

Grewal S, Morrison EE, Ponnambalam S, Walker JH Nuclear localisation of cytosolic phospholipase A(2)-alpha in the EA.hy.926 human endothelial cell line is proliferation dependent and modulated by phosphorylation J CELL SCI 115 4533-4543, 2002
DOI:10.1242/jcs.00148

Cobbold C, Ponnambalam S, Francis MJ, Monaco AP Novel membrane traffic steps regulate the exocytosis of the Menkes disease ATPase HUM MOL GENET 11 2855-2866, 2002

McCrossan M, Windsor M, Ponnambalam S, Armstrong J, Wileman T The trans Golgi network is lost from cells infected with African swine fever virus. J Virol 75 11755-11765, 2001
DOI:10.1128/JVI.75.23.11755-11765.2001
View abstract

Prescott AR, Farmaki T, Thomson C, James J, Paccaud JP, Tang BL, Hong WJ, Quinn M, Ponnambalam S, Lucocq J Evidence for prebudding arrest of ER export in animal cell mitosis and its role in generating golgi partitioning intermediates TRAFFIC 2 321-335, 2001

McLauchlan HJ, James J, Lucocq JM, Ponnambalam S Characterization and regulation of constitutive transport intermediates involved in trafficking from the trans-Golgi network. Cell Biol Int 25 705-713, 2001
DOI:10.1006/cbir.2001.0717
View abstract

Danzeisen R, Ponnambalam S, Lea RG, Page K, Gambling L, McArdle HJ The effect of ceruloplasmin on iron release from placental (BeWo) cells: Evidence for an endogenous Cu oxidase PLACENTA 21 805-812, 2000

Towler MC, Prescott AR, James J, Lucocq JM, Ponnambalam S The manganese cation disrupts membrane dynamics along the secretory pathway EXP CELL RES 259 167-179, 2000
DOI:10.1006/excr.2000.4958

Massat N, Ponnambalam S, Watts C Processing of apoptotic/necrotic cell derived antigens expressed in different cellular compartments by murine Dendritic Cells. FASEB J 14 A1052-A1052, 2000

Ponnambalam S, Clough S, Downes CP, Lucocq JM, McLauchlan HJ, Towler MC Lipid kinases and trans-Golgi network membrane dynamics. Biochem Soc Trans 27 670-673, 1999

Francis MJ, Jones EE, Levy ER, Martin RL, Ponnambalam S, Monaco AP Identification of a di-leucine motif within the C terminus domain of the Menkes disease protein that mediates endocytosis from the plasma membrane J CELL SCI 112 1721-1732, 1999

Farmaki T, Ponnambalam S, Prescott AR, Clausen H, Tang BL, Hong WJ, Lucocq JM Forward and retrograde trafficking in mitotic animal cells - ER-Golgi transport arrest restricts protein export from the ER into COPII-coated structures J CELL SCI 112 589-600, 1999

Francis MJ, Jones EE, Levy ER, Ponnambalam S, Chelly J, Monaco AP A Golgi localization signal identified in the Menkes recombinant protein HUM MOL GENET 7 1245-1252, 1998

Hickinson DM, Lucocq JM, Towler MC, Clough S, James J, James SR, Downes CP, Ponnambalam S Association of a phosphatidylinositol-specific 3-kinase with a human trans-Golgi network resident protein CURR BIOL 7 987-990, 1997

Knight AM, Lucocq JM, Prescott AR, Ponnambalam S, Watts C Antigen endocytosis and presentation mediated by human membrane IgG1 in the absence of the Ig(alpha)/Ig(beta) dimer. EMBO J 16 3842-3850, 1997
DOI:10.1093/emboj/16.13.3842
View abstract

Prescott AR, Lucocq JM, James J, Lister JM, Ponnambalam S Distinct compartmentalization of TGN46 and beta 1,4-galactosyltransferase in HeLa cells EUR J CELL BIOL 72 238-246, 1997

Banting G, Ponnambalam S TGN38 and its orthologues: Roles in post-TGN vesicle formation and maintenance of TGN morphology BBA-MOL CELL RES 1355 209-217, 1997

Ponnambalam S, Banting G Protein secretion: Sorting sweet sorting CURR BIOL 6 1076-1078, 1996

Copier J, Kleijmeer MJ, Ponnambalam S, Oorschot V, Potter P, Trowsdale J, Kelly A Targeting signal and subcellular compartments involved in the intracellular trafficking of HLA-DMB J IMMUNOL 157 1017-1027, 1996

Ponnambalam S, Girotti M, Yaspo ML, Owen CE, Perry ACF, Suganuma T, Nilsson T, Fried M, Ranting G, Warren G Primate homologues of rat TGN38: Primary structure, expression and functional implications J CELL SCI 109 675-685, 1996

PONNAMBALAM S, JACKSON AP, LEBEAU MM, PRAVTCHEVA D, RUDDLE FH, ALIBERT C, PARHAM P CHROMOSOMAL LOCATION AND SOME STRUCTURAL FEATURES OF HUMAN CLATHRIN LIGHT-CHAIN GENES (CLTA AND CLTB) GENOMICS 24 440-444, 1994

Ponnambalam S, Rabouille C, Luzio JP, Nilsson T, Warren G The TGN38 glycoprotein contains two non-overlapping signals that mediate localization to the trans-Golgi network. Journal of Cell Biology 125 253-268, 1994

Armstrong J, Craighead MW, Watson R, Ponnambalam S, Bowden S Schizosaccharomyces pombe ypt5 : a homologue of the rab5 endosome fusion regulator. Molecular Biology of the Cell 4 583-592, 1993

BRODSKY FM, HILL BL, ACTON SL, NATHKE I, WONG DH, PONNAMBALAM S, PARHAM P CLATHRIN LIGHT-CHAINS - ARRAYS OF PROTEIN MOTIFS THAT REGULATE COATED-VESICLE DYNAMICS TRENDS BIOCHEM SCI 16 208-213, 1991

PONNAMBALAM S, ROBINSON MS, JACKSON AP, PEIPERL L, PARHAM P CONSERVATION AND DIVERSITY IN FAMILIES OF COATED VESICLE ADAPTINS J BIOL CHEM 265 4814-4820, 1990

PONNAMBALAM S, CHAN B, BUSBY S FUNCTIONAL-ANALYSIS OF DIFFERENT SEQUENCE ELEMENTS IN THE ESCHERICHIA-COLI GALACTOSE OPERON P2 PROMOTER MOL MICROBIOL 2 165-172, 1988

PONNAMBALAM S, SPASSKY A, BUSBY S STUDIES WITH THE ESCHERICHIA-COLI GALACTOSE OPERON REGULATORY REGION CARRYING A POINT MUTATION THAT SIMULTANEOUSLY INACTIVATES THE 2 OVERLAPPING PROMOTERS - INTERACTIONS WITH RNA-POLYMERASE AND THE CYCLIC-AMP RECEPTOR PROTEIN FEBS LETT 219 189-196, 1987

JOHNSTON F, PONNAMBALAM S, BUSBY S BINDING OF ESCHERICHIA-COLI RNA-POLYMERASE TO A PROMOTER CARRYING MUTATIONS THAT STOP TRANSCRIPTION INITIATION J MOL BIOL 195 745-748, 1987

PONNAMBALAM S, BUSBY S RNA-POLYMERASE MOLECULES INITIATING TRANSCRIPTION AT TANDEM PROMOTERS CAN COLLIDE AND CAUSE PREMATURE TRANSCRIPTION TERMINATION FEBS LETT 212 21-27, 1987

PONNAMBALAM S, WEBSTER C, BINGHAM A, BUSBY S TRANSCRIPTION INITIATION AT THE ESCHERICHIA-COLI GALACTOSE OPERON PROMOTERS IN THE ABSENCE OF THE NORMAL-35 REGION SEQUENCES J BIOL CHEM 261 6043-6048, 1986

BINGHAM AHA, PONNAMBALAM S, CHAN B, BUSBY S MUTATIONS THAT REDUCE EXPRESSION FROM THE P2 PROMOTER OF THE ESCHERICHIA-COLI GALACTOSE OPERON GENE 41 67-74, 1986