Faculty of Biological Sciences

Dr Alex O'Neill

BSc, Bristol; PhD 2001, Leeds
Associate Professor
School of Molecular and Cellular Biology

Contact:  Garstang 8.52c, +44(0) 113 34 35600, email address for  

You can read more about Dr O'Neill's interests here:

Research Interests

Dissecting the molecular mechanisms of antibiotic resistance in bacteria, and discovery of new antibacterial drugs

Antibiotics enable the treatment and cure of life-threatening bacterial infections, and represent one of the great successes of modern medicine. Unfortunately, the utility of these agents is being progressively eroded as bacteria evolve to resist their effects.

My laboratory seeks to better understand and address this problem through a combination of fundamental and applied research. Whilst our core expertise is molecular microbiology, we favour a multi-disciplinary approach that involves working closely with medicinal chemists, structural biologists and clinical scientists.

Research in the laboratory is funded by the Research Councils (MRC, BBSRC), the EU Framework 7 Programme, and industry (Cubist Pharmaceuticals, Helperby Therapeutics, Smith & Nephew, Syntopix).

 

The prototype self-transmissible antimicrobial
resistance plasmid from Staphyloccus aureus

Current main areas of focus are;

(i) The molecular mechanisms of resistance to antibiotics, particularly in the ‘superbug’, Staphylococcus aureus

  • Investigating the genetic basis for the emergence and maintenance of resistance and tolerance to antibiotics
  • Characterization of genetic elements involved in spreading antibiotic resistance
  • Understanding the biochemical and structural basis for antibiotic resistance


Cells of Staphylococcus aureus bursting
following exposure to antibiotics(ii) Identification and biological characterization of antibacterial agents

  • Discovery and design of novel antibacterial agents (in collaboration with colleagues in the School of Chemistry)
  • Biological characterization of both novel and established antibacterial agents
  • Development of novel tools and approaches to elucidate antibiotic mode of action

 

 

Current Projects

Post-doctoral appointments

Posts for which funding has already been secured (where available) will be advertised on www.jobs.ac.uk.

Those wishing to apply for a Fellowship to join the O’Neill lab are encouraged to get in touch to discuss possibilities. A non-exhaustive list of organizations that offer Fellowships in biological sciences is provided below;

AXA

EMBO

HFSP

Marie Skłodowska-Curie

Newton

NIH

Wellcome Trust

 

Faculty Research and Innovation



Studentship information

Postgraduate studentship areas:

  • http://www.findaphd.com/search/ProjectDetails.aspx?PJID=24409&LID=735
  • http://www.findaphd.com/search/showproject.asp?projectid=11830

See also:

Modules managed

BIOL5296M - Treatment of Infectious Disease and Cancer
BIOL9001 - Study Year Abroad
BMSC2210 - Chemotherapy
MICR2220 - Medical Bacteriology

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC2301 - Intermediate Integrated Biochemistry
BIOC2303 - Intermediate Biochemistry: Skills
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOL2110 - The Power of Bacterial Genomics
BIOL2301 - Intermediate Skills for Biological Sciences
BIOL2301/03/05/MICR2320 - Intermediate Skills - BIOL/MICR
BIOL3306 - Biological Sciences Research Project
BIOL3399 - Extended Research Project Preparation
BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5296M - Treatment of Infectious Disease and Cancer
BIOL5373M - Protein Engineering Laboratory Project
BIOL5382M - Extended Research Project
BIOL5392M - Bioscience MSc Research Project
BIOL5394M - Specialised Research Topics and Skills
BMSC2210 - Chemotherapy
FOBS1135/BIOL1112 - The Basis of Life/The molecules of life
MICR1120 - Introduction to Microbiology
MICR2220 - Medical Bacteriology
MICR2320 - Skills for Microbiologists 2
MICR3120/BIOC3111/12/BIOL3112 A - ATU - Respiratory infections
MICR3120/BIOL3112/BMSC3143/46 - ATU - Antibiotics action and resistance
MICR3325 - Skills for Microbiologists 3

Academic roles

Study Abroad Tutor
Examinations Officer for PGT Programmes - PGT MCB programmes
Examinations Officer for UG Programmes - UG MCB programmes

Committees

Member of Undergraduate School Taught Student Education Committee

Centre memberships:

Group Leader Dr Alex O'Neill  (Associate Professor)

Dissecting the molecular mechanisms of antibiotic resistance in bacteria, and discovery of new antibacterial drugs 

Dr Louise Kime  (Research Fellow)

Dr Christopher Randall  (Teaching Fellow)

Dr Jennifer Tomlinson  (Research Fellow)

Mr John Wright  (Technician)


Postgraduates

Asma Akter (Primary supervisor) 70% FTE
Fawaz Almufarriji (Primary supervisor) 100% FTE
Zeyad Alzeyadi (Primary supervisor) 90% FTE
Elham Elkrewi (Primary supervisor) 90% FTE
Luiza Galarion (Primary supervisor) 100% FTE
Ryan Gonciarz (Primary supervisor) 50% FTE
Arya Gupta (Primary supervisor) 50% FTE
Nicole Jackson (Primary supervisor) 60% FTE
Aisha Kalendar (Primary supervisor) 100% FTE
Victoria Lee (Primary supervisor) 90% FTE
Jennifer Mitchell (Primary supervisor) 50% FTE
Merianne Mohamad (Primary supervisor) 100% FTE
Fatma Salama (Primary supervisor) 50% FTE
Lia De Faveri (Co-supervisor) 20% FTE
David Nicholson (Co-supervisor) 33% FTE
Virginia Pappa (Co-supervisor) 50% FTE

Elkrewi E, Randall CP, Ooi N, Cottell JL, O'Neill AJ Cryptic silver resistance is prevalent and readily activated in certain Gram-negative pathogens JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY 72 3043-3046, 2017
DOI:10.1093/jac/dkx258

Renzoni A, Von Dach E, Landelle C, Diene SM, Manzano C, Gonzales R, Abdelhady W, Randall CP, Bonetti EJ, Baud D, O'Neill AJ, Bayer A, Cherkaoui A, Schrenzel J, Harbarth S, François P Impact of exposure of methicillin-resistant Staphylococcus aureus to polyhexanide in vitro and in vivo. Antimicrobial Agents and Chemotherapy 61 -, 2017
DOI:10.1128/AAC.00272-17
View abstract

Ooi N, O'Neill AJ Revisiting unexploited antibiotics in search of new antibacterial drug candidates: the case of MSD-819 (6-chloro-2-quinoxalinecarboxylic acid 1,4-dioxide) JOURNAL OF ANTIBIOTICS 70 317-319, 2017
DOI:10.1038/ja.2016.140

Lee VE, O'Neill AJ Batumin does not exert its antistaphylococcal effect through inhibition of aminoacyl-tRNA synthetase enzymes. Int J Antimicrob Agents 49 121-122, 2017
DOI:10.1016/j.ijantimicag.2016.10.005

Randall CP, Rasina D, Jirgensons A, O'Neill AJ Targeting Multiple Aminoacyl-tRNA Synthetases Overcomes the Resistance Liabilities Associated with Antibacterial Inhibitors Acting on a Single Such Enzyme Antimicrobial Agents and Chemotherapy 60 6359-6361, 2016
DOI:10.1128/AAC.00674-16
View abstract

Ooi N, Eady EA, Cove JH, O'Neill AJ Tert-butyl benzoquinone: mechanism of biofilm eradication and potential for use as a topical antibiofilm agent Journal of Antimicrobial Chemotherapy 71 1841-1844, 2016
DOI:10.1093/jac/dkw100
View abstract

Gupta A, Monteferrante C, Rasina D, Leitis G, Randall CP, Tomlinson JH, Jirgensons A, Goessens WHF, Hays JP, O'Neill AJ A polymorphism in leuS confers reduced susceptibility to GSK2251052 in a clinical isolate of Staphylococcus aureus Antimicrobial Agents and Chemotherapy 60 3219-3221, 2016
DOI:10.1128/AAC.02940-15
View abstract

Gerits E, Blommaert E, Lippell A, O'Neill AJ, Weytjens B, De Maeyer D, Fierro AC, Marchal K, Marchand A, Chaltin P, Spincemaille P, De Brucker K, Thevissen K, Cammue BP, Swings T, Liebens V, Fauvart M, Verstraeten N, Michiels J Elucidation of the Mode of Action of a New Antibacterial Compound Active against Staphylococcus aureus and Pseudomonas aeruginosa. PloS one 11 e0155139-e0155139, 2016
DOI:10.1371/journal.pone.0155139
View abstract

Sharkey LKR, Edwards TA, O Neill AJ ABC-F proteins mediate antibiotic resistance through ribosomal protection mBio 7 -, 2016
DOI:10.1128/mBio.01975-15
View abstract

Tomlinson JH, Thompson GS, Kalverda AP, Zhuravleva A, O'Neill AJ A target-protection mechanism of antibiotic resistance at atomic resolution: Insights into FusB-type fusidic acid resistance Scientific Reports 6 -, 2016
DOI:10.1038/srep19524
View abstract

Jiang Y, O'Neill AJ, Ding Y Zinc oxide nanoparticle-coated films: fabrication, characterization, and antibacterial properties Journal of Nanoparticle Research 17 -, 2015
DOI:10.1007/s11051-015-2993-6
View abstract

Ooi N, Eady EA, Cove JH, O'Neill AJ Redox-active compounds with a history of human use: antistaphylococcal action and potential for repurposing as topical antibiofilm agents Journal of Antimicrobial Chemotherapy 70 479-488, 2015
DOI:10.1093/jac/dku409
View abstract

Randall C, Gupta A, Jackson N, Busse D, O'Neill AJ Silver resistance in Gram-negative bacteria: a dissection of endogenous and exogenous mechanisms Journal of Antimicrobial Chemotherapy 70 1037-1046, 2015
DOI:10.1093/jac/dku523
View abstract

Liebens V, Gerits E, Knapen WJ, Swings T, Beullens S, Steenackers HP, Robijns S, Lippell A, O'Neill AJ, Veber M, Fröhlich M, Krona A, Lövenklev M, Corbau R, Marchand A, Chaltin P, De Brucker K, Thevissen K, Cammue BP, Fauvart M, Verstraeten N, Michiels J Identification and characterization of an anti-pseudomonal dichlorocarbazol derivative displaying anti-biofilm activity Bioorganic and Medicinal Chemistry Letters 24 5404-5408, 2014
DOI:10.1016/j.bmcl.2014.10.039
View abstract

Hrast M, Anderluh M, Knez D, Randall CP, Barreteau H, O'Neill AJ, Blanot D, Gobec S Design, synthesis and evaluation of second generation MurF inhibitors based on a cyanothiophene scaffold. Eur J Med Chem 73 83-96, 2014
DOI:10.1016/j.ejmech.2013.11.031
View abstract

Cox G, Edwards TA, O'Neill AJ Mutagenesis mapping of the protein-protein interaction underlying FusB-type Fusidic acid resistance Antimicrobial Agents and Chemotherapy 57 4640-4644, 2013
DOI:10.1128/AAC.00198-13
View abstract

Škedelj V, Perdih A, Brvar M, Kroflič A, Dubbée V, Savage V, O'Neill AJ, Solmajer T, Bešter-Rogač M, Blanot D, Hugonnet J-E, Magnet S, Arthur M, Mainardi J-L, Stojan J, Zega A Discovery of the first inhibitors of bacterial enzyme D-aspartate ligase from Enterococcus faecium (Aslfm). Eur J Med Chem 67 208-220, 2013
DOI:10.1016/j.ejmech.2013.06.017
View abstract

Hrast M, Turk S, Sosič I, Knez D, Randall CP, Barreteau H, Contreras-Martel C, Dessen A, O'Neill AJ, Mengin-Lecreulx D, Blanot D, Gobec S Structure-activity relationships of new cyanothiophene inhibitors of the essential peptidoglycan biosynthesis enzyme MurF. Eur J Med Chem 66 32-45, 2013
DOI:10.1016/j.ejmech.2013.05.013
View abstract

Ooi N, Chopra I, Eady A, Cove J, Bojar R, O'Neill AJ Antibacterial activity and mode of action of tert-butylhydroquinone (TBHQ) and its oxidation product, tert-butylbenzoquinone (TBBQ). J Antimicrob Chemother 68 1297-1304, 2013
DOI:10.1093/jac/dkt030
View abstract

Savage VJ, Chopra I, O'Neill AJ Population diversification in Staphylococcus aureus biofilms may promote dissemination and persistence. PLoS One 8 -, 2013
DOI:10.1371/journal.pone.0062513
View abstract

Savage VJ, Chopra I, O'Neill AJ Staphylococcus aureus biofilms promote horizontal transfer of antibiotic resistance. Antimicrob Agents Chemother 57 1968-1970, 2013
DOI:10.1128/AAC.02008-12
View abstract

Randall CP, Mariner KR, Chopra I, O'Neill AJ The target of daptomycin is absent from Escherichia coli and other gram-negative pathogens. Antimicrob Agents Chemother 57 637-639, 2013
DOI:10.1128/AAC.02005-12
View abstract

Randall CP, Oyama LB, Bostock JM, Chopra I, O'Neill AJ The silver cation (Ag+): antistaphylococcal activity, mode of action and resistance studies. J Antimicrob Chemother 68 131-138, 2013
DOI:10.1093/jac/dks372
View abstract

Blake KL, O'Neill AJ Transposon library screening for identification of genetic loci participating in intrinsic susceptibility and acquired resistance to antistaphylococcal agents. J Antimicrob Chemother 68 12-16, 2013
DOI:10.1093/jac/dks373
View abstract

Caryl JA, Cox G, Trimble S, O'Neill AJ "tet(U)" is not a tetracycline resistance determinant. Antimicrob Agents Chemother 56 3378-3379, 2012
DOI:10.1128/AAC.05957-11
View abstract

Rosado H, O'Neill AJ, Blake KL, Walther M, Long PF, Hinds J, Taylor PW Rotating wall vessel exposure alters protein secretion and global gene expression in Staphylococcus aureus International Journal of Astrobiology 11 71-81, 2012
DOI:10.1017/S1473550411000346
View abstract

Cox G, Thompson GS, Jenkins HT, Peske F, Savelsbergh A, Rodnina MV, Wintermeyer W, Homans SW, Edwards TA, O'Neill AJ Ribosome clearance by FusB-type proteins mediates resistance to the antibiotic fusidic acid. Proc Natl Acad Sci U S A 109 2102-2107, 2012
DOI:10.1073/pnas.1117275109
View abstract

Griffiths JM, O'Neill AJ Loss of function of the gdpP protein leads to jointβ-lactam/glycopeptide tolerance in Staphylococcus aureus. Antimicrob Agents Chemother 56 579-581, 2012
DOI:10.1128/AAC.05148-11
View abstract

Škedelj V, Arsovska E, Tomašić T, Kroflič A, Hodnik V, Hrast M, Bešter-Rogač M, Anderluh G, Gobec S, Bostock J, Chopra I, O'Neill AJ, Randall C, Zega A 6-Arylpyrido[2,3-d]pyrimidines as novel ATP-competitive inhibitors of bacterial D-alanine:D-alanine ligase. PLoS One 7 e39922-, 2012
DOI:10.1371/journal.pone.0039922
View abstract

Ryder VJ, Chopra I, O'Neill AJ Increased mutability of Staphylococci in biofilms as a consequence of oxidative stress. PLoS One 7 e47695-, 2012
DOI:10.1371/journal.pone.0047695
View abstract

McPhillie MJ, Trowbridge R, Mariner KR, O'Neill AJ, Johnson AP, Chopra I, Fishwick CWG Structure-Based Ligand Design of Novel Bacterial RNA Polymerase Inhibitors ACS MED CHEM LETT 2 729-734, 2011
DOI:10.1021/ml200087m

Vehar B, Hrast M, Kovac A, Konc J, Mariner K, Chopra I, O'Neill A, Janezic D, Gobec S Ellipticines and 9-acridinylamines as inhibitors of D-alanine:D-alanine ligase BIOORGAN MED CHEM 19 5137-5146, 2011
DOI:10.1016/j.bmc.2011.07.020

O'Neill AJ Bacterial phenotypes refractory to antibiotic-mediated killing: mechanisms and mitigation In Emerging Trends in Antibacterial Discovery: Answering the Call to Arms , 2011

Blake KL, Randall CP, O'Neill AJ In Vitro Studies Indicate a High Resistance Potential for the Lantibiotic Nisin in Staphylococcus aureus and Define a Genetic Basis for Nisin Resistance ANTIMICROB AGENTS CH 55 2362-2368, 2011
DOI:10.1128/AAC.01077-10

Mariner K, McPhillie M, Trowbridge R, Smith C, O'Neill AJ, Fishwick CWG, Chopra I Activity of and Development of Resistance to Corallopyronin A, an Inhibitor of RNA Polymerase ANTIMICROB AGENTS CH 55 2413-2416, 2011
DOI:10.1128/AAC.01742-10

Mariner KR, Ooi N, Roebuck D, O'Neill AJ, Chopra I Further Characterization of Bacillus subtilis Antibiotic Biosensors and Their Use for Antibacterial Mode-of-Action Studies ANTIMICROB AGENTS CH 55 1784-1786, 2011
DOI:10.1128/AAC.01710-10

McLaws FB, Larsen AR, Skov RL, Chopra I, O'Neill AJ Distribution of Fusidic Acid Resistance Determinants in Methicillin-Resistant Staphylococcus aureus ANTIMICROB AGENTS CH 55 1173-1176, 2011
DOI:10.1128/AAC.00817-10

Hurdle JG, O'Neill AJ, Chopra I, Lee RE Targeting bacterial membrane function: an underexploited mechanism for treating persistent infections NAT REV MICROBIOL 9 62-75, 2011
DOI:10.1038/nrmicro2474

Mariner KR, Trowbridge R, Agarwal AK, Miller K, O'Neill AJ, Fishwick CWG, Chopra I Furanyl-Rhodanines Are Unattractive Drug Candidates for Development as Inhibitors of Bacterial RNA Polymerase ANTIMICROB AGENTS CH 54 4506-4509, 2010
DOI:10.1128/AAC.00753-10

O'Neill AJ Staphylococcus aureus SH1000 and 8325-4: comparative genome sequences of key laboratory strains in staphylococcal research LETT APPL MICROBIOL 51 358-361, 2010
DOI:10.1111/j.1472-765X.2010.02885.x

Zhang LL, Jiang YH, Ding YL, Daskalakis N, Jeuken L, Povey M, O'Neill AJ, York DW Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli J NANOPART RES 12 1625-1636, 2010
DOI:10.1007/s11051-009-9711-1

Caryl JA, O'Neill AJ Complete nucleotide sequence of pGO1, the prototype conjugative plasmid from the staphylococci PLASMID 62 35-38, 2009
DOI:10.1016/j.plasmid.2009.03.001

Vickers AA, Potter NJ, Fishwick CWG, Chopra I, O'Neill AJ Analysis of mutational resistance to trimethoprim in Staphylococcus aureus by genetic and structural modelling techniques J ANTIMICROB CHEMOTH 63 1112-1117, 2009
DOI:10.1093/jac/dkp090

Blake KL, O'Neill AJ, Mengin-Lecreulx D, Henderson PJF, Bostock JM, Dunsmore CJ, Simmons KJ, Fishwick CWG, Leeds JA, Chopra I The nature of Staphylococcus aureus MurA and MurZ and approaches for detection of peptidoglycan biosynthesis inhibitors MOL MICROBIOL 72 335-343, 2009
DOI:10.1111/j.1365-2958.2009.06648.x

O'Neill AJ, Lindsay JA, Gould K, Hinds J, Chopra I Transcriptional Signature following Inhibition of Early-Stage Cell Wall Biosynthesis in Staphylococcus aureus ANTIMICROB AGENTS CH 53 1701-1704, 2009
DOI:10.1128/AAC.01309-08

Hobbs JK, Miller K, O'Neill AJ, Chopra I Consequences of daptomycin-mediated membrane damage in Staphylococcus aureus Journal of Antimicrobial Chemotherapy 62 1003-1008, 2008
DOI:10.1093/jac/dkn321

Miller K, O'Neill AJ, Wilcox MH, Ingham E, Chopra I Delayed development of linezolid resistance in Staphylococcus aureus following exposure to low levels of antimicrobial agents ANTIMICROB AGENTS CH 52 1940-1944, 2008
DOI:10.1128/AAC.01302-07

McLaws F, Chopra I, O'Neill AJ High prevalence of resistance to fusidic acid in clinical isolates of Staphylococcus epidermidis J ANTIMICROB CHEMOTH 61 1040-1043, 2008
DOI:10.1093/jac/dkn071

Driffield K, Miller K, Bostock JM, O'Neill AJ, Chopra I Increased mutability of Pseudomonas aeruginosa in biofilms J ANTIMICROB CHEMOTH 61 1053-1056, 2008
DOI:10.1093/jac/dkn044

O'Neill AJ New antibacterial agents for treating infections caused by multi-drug resistant Gram-negative bacteria. Expert Opin Investig Drugs 17 297-302, 2008
DOI:10.1517/13543784.17.3.297
View abstract

Chopra I, Schofield C, Everett M, O'Neill A, Miller K, Wilcox M, Frere JM, Dawson M, Czapiewski L, Urleb U, Courvalin P Treatment of health-care-associated infections caused by Gram-negative bacteria: a consensus statement LANCET INFECT DIS 8 133-139, 2008

Vickers AA, Chopra I, O'Neill AJ Intrinsic novobiocin resistance in Staphylococcus saprophyticus ANTIMICROB AGENTS CH 51 4484-4485, 2007
DOI:10.1128/AAC.00708-07

Vickers AA, O'Neill AJ, Chopra I Emergence and maintenance of resistance to fluoroquinolones and coumarins in Staphylococcus aureus: predictions from in vitro studies J ANTIMICROB CHEMOTH 60 269-273, 2007
DOI:10.1093/jac/dkm19l

O'Neill AJ, Larsen AR, Skov R, Henriksen AS, Chopra I Characterization of the epidemic European fusidic acid-resistant impetigo clone of Staphylococcus aureus J CLIN MICROBIOL 45 1505-1510, 2007
DOI:10.1128/JCM.01984-06

O'Neill AJ, McLaws F, Kahlineter G, Henriksen AS, Chopra I Genetic basis of resistance to fusidic acid in staphylococci ANTIMICROB AGENTS CH 51 1737-1740, 2007
DOI:10.1128/AAC.01542-06

Driffield KL, Bostock JM, Miller K, O'Neill AJ, Hobbs JK, Chopra I Evolution of extended-spectrum beta-lactamases in a MutS-deficient Pseudomonas aeruginosa hypermutator Journal of Antimicrobial Chemotherapy 58 905-907, 2006

O'Neill AJ, Chopra I Molecular basis of fusB-mediated resistance to fusidic acid in Staphylococcus aureus. Mol Microbiol 59 664-676, 2006
DOI:10.1111/j.1365-2958.2005.04971.x
View abstract

O'Neill AJ, Huovinen T, Fishwick CWG, Chopra I Molecular Genetic and Structural Modeling Studies of Staphylococcus aureus RNA Polymerase and the Fitness of Rifampin Resistance Genotypes in Relation to Clinical Prevalence Antimicrobial Agents&Chemotherapy 50 298-309, 2006
DOI:10.1128/AAC.50.1.298-309.2006
View abstract

Hurdle JG, O'Neill AJ, Chopra I Prospects for aminoacyl-tRNA synthetase inhibitors as new antimicrobial agents. Antimicrob Agents Chemother 49 4821-4833, 2005
DOI:10.1128/AAC.49.12.4821-4833.2005

Hurdle JG, O'Neill AJ, Mody L, Chopra I, Bradley SF In vivo transfer of high-level mupirocin resistance from Staphylococcus epidermidis to methicillin-resistant Staphylococcus aureus associated with failure of mupirocin prophylaxis. J Antimicrob Chemother 56 1166-1168, 2005
DOI:10.1093/jac/dki387
View abstract

O'Neill AJ, Miller K, Oliva B, Chopra I Comparison of assays for detection of agents causing membrane damage in Staphylococcus aureus J ANTIMICROB CHEMOTH 54 1127-1129, 2004
DOI:10.1093/jac/dkh476

Hurdle JG, O'Neill AJ, Ingham E, Fishwick C, Chopra I Analysis of mupirocin resistance and fitness in Staphylococcus aureus by molecular genetic and structural modeling techniques ANTIMICROB AGENTS CH 48 4366-4376, 2004
DOI:10.1128/AAC.48.11.4366-4376.2004

O'Neill AJ, Larsen AR, Henriksen AS, Chopra I A fusidic acid-resistant epidemic strain of Staphylococcus aureus carries the fusB determinant, whereas fusA mutations are prevalent in other resistant isolates ANTIMICROB AGENTS CH 48 3594-3597, 2004
DOI:10.1128/AAC.48.9.3594-3597.2004

Hurdle JG, O'Neill AJ, Chopra I Anti-staphylococcal activity of indolmycin, a potential topical agent for control of staphylococcal infections J ANTIMICROB CHEMOTH 54 549-552, 2004
DOI:10.1093/jac/dkh352

O'Neill AJ, Chopra I Preclinical evaluation of novel antibacterial agents by microbiological and molecular techniques EXPERT OPIN INV DRUG 13 1045-1063, 2004

Oliva B, O'Neill AJ, Miller K, Stubbings WJ, Chopra I Anti-staphylococcal activity and mode of action of clofazimine Journal of Antimicrobial Chemotherapy 53 435-440, 2004
DOI:10.1093/jac/dkh114
View abstract

Hurdle JG, O'Neill AJ, Chopra I The isoleucyl-tRNA synthetase mutation V588F conferring mupirocin resistance in glycopeptide-intermediate Staphylococcus aureus is not associated with a significant fitness burden. J Antimicrob Chemother 53 102-104, 2004
DOI:10.1093/jac/dkh020
View abstract

Miller K, O'Neill AJ, Chopra I Escherichia coli mutators present an enhanced risk for emergence of antibiotic resistance during urinary tract infections ANTIMICROB AGENTS CH 48 23-29, 2004
DOI:10.1128/AAC.48.1.23-29.2004

Chopra I, O'Neill AJ, Miller K The role of mutators in the emergence of antibiotic-resistant bacteria DRUG RESIST UPDATE 6 137-145, 2003
DOI:10.1016/S1368-7646(03)00041-4

Bostock JM, Miller K, O'Neill AJ, Chopra I Zeocin resistance suppresses mutation in hypermutable Escherichia coli MICROBIOL-SGM 149 815-816, 2003
DOI:10.1099/mic.0.C0111-0

Oliva B, Miller K, Caggiano N, O'Neill AJ, Cuny GD, Hoemann MZ, Hauske JR, Chopra I Biological properties of novel antistaphylococcal quinoline-indole agents. Antimicrob Agents Chemother 47 458-466, 2003
View abstract

O'Neill AJ, Chopra I Lack of evidence for involvement of hypermutability in emergence of vancomycin-intermediate Staphylococcus aureus Antimicrobial Agents&Chemotherapy 47 1484-1485, 2003

O'Neill AJ, Bostock JM, Moita AM, Chopra I Antimicrobial activity and mechanisms of resistance to cephalosporin P1, an antibiotic related to fusidic acid. J Antimicrob Chemother 50 839-848, 2002
View abstract

O'Neill AJ, Chopra I Insertional inactivation of mutS in Staphylococcus aureus reveals potential for elevated mutation frequencies, although the prevalence of mutators in clinical isolates is low J ANTIMICROB CHEMOTH 50 161-169, 2002
DOI:10.1093/jac/dkf118

Miller K, O'Neill AJ, Chopra I Response of Escherichia coli hypermutators to selection pressure with antimicrobial agents from different classes J ANTIMICROB CHEMOTH 49 925-934, 2002
DOI:10.1093/jac/dkf044

Chopra I, Hesse L, O'Neill AJ Exploiting current understanding of antibiotic action for discovery of new drugs. J Appl Microbiol 92 Suppl 4S-15S, 2002
DOI:10.1046/j.1365-2672.92.5s1.13.x
View abstract

M'Zali FH, O'Neill AJ, Hawkey PM, Dalhoff A, Ambler JE Examination of the spectrum of mutations involved in resistance to fluoroquinolones in Staphylococcus aureus by a combination of polymerase chain reaction (PCR) and denaturing high performance liquid chromatography (DHPLC) J ANTIMICROB CHEMOTH 47 36-36, 2001

O'Neill AJ, Chopra I Use of mutator strains for characterization of novel antimicrobial agents ANTIMICROB AGENTS CH 45 1599-1599, 2001

O'Neill AJ, Cove JH, Chopra I Mutation frequencies for resistance to fusidic acid and rifampicin in Staphylococcus aureus Journal of Antimicrobial Chemotherapy 47 647-650, 2001
DOI:10.1093/jac/47.5.647

Oliva B, O'Neill AJ, Wilson JM, O'Hanlon PJ, Chopra I Antimicrobial properties and mode of action of the pyrrothine holomycin International Journal of Antimicrobial Agents and Chemotherapy 45 532-539, 2001
DOI:10.1128/AAC.45.2.532-539.2001

O'Neill A, Oliva B, Storey C, Hoyle A, Fishwick C, Chopra I RNA polymerase inhibitors with activity against rifampin-resistant mutants of Staphylococcus aureus ANTIMICROB AGENTS CH 44 3163-3166, 2000