Dr Stephen Muench

PhD 2004; BSc (Hons), Sheffield
Lecturer in Membrane Biology
School of Biomedical Sciences

Contact: Astbury 6.106, +44(0) 113 34 34279, email address for  

You can read more about Dr Muench's interests here:

Research Interests

Structure, function and molecular mechanism of large protein complexes

Overall Goal

One of the major challenges for structural biology will be not just to resolve the structures of large protein complexes, but crucially also to appreciate the dynamics and conformational variability of such systems. These challenges are best met through a combination of techniques such as X-ray crystallography and electron microscopy, in particular time-resolved applications. My research involves the combination of these techniques in order to understand the structure/function relationship of a number of medically important targets, with a particular interest in membrane protiens.

Structure, function and molecular mechanism of the vacuolar ATPase

The vacuolar H+-ATPase (V-ATPase) is an ATP-driven proton pump essential for the function of virtually all eukaryotic cells. It is a membrane-bound rotary motor, larger and more complex than the related ATPase synthase, and is responsible for acidifying intracellular compartments, energising membrane transport and pH homeostasis. Mutations in V-ATPase genes cause osteopetrosis, kidney disease and autophagic myopathy, and its involvement in osteoporosis and metastatic cancer means that controlling its activity with drugs has therapeutic potential.


 Figure1. A) M. sexta V-ATPase single particle cryo-EM reconstruction with subunit homologues fitted, B) Negative stain alalysis of the V-ATPase bound to a Pa1b-Strep-HRP complex, highlighted by an arrow (scale bar is 10nm). C) Slice through of the EM map of the catalytic V1 domain after accounting for flexibility in the complex.

Despite its importance, our understanding of the V-ATPase mechanism and regulation is poor and is a major block in therapeutic development, since structural data often underpins therapeutic drug design. We have now solved the first sub nm structure of the V-ATPase by single particle cryo-EM (Figure 1A), providing new insights into its structure and mechanism. Moreover, using a novel tagging mechanism we have studied the binding of the selective inhibitor PA1b to the V-ATPase (Figure 1B). Through accounting for the inherent flexibility within the system, in particular of the V1 and Vo domains its also possible improve the resulting resolution of the EM reconstruction (Figure 1C).

This complex motor is regulated in response to physiological signals indicating low energy, leading to reversible domain dissociation. The nature of the structural changes that occur in response to these signals is being studied by single particle cryo-EM and, where appropriate, crystallography.


Further Areas of Research

In addition to working on the V-ATPase we are working on a number of therapeutic targets both membrane bound and soluble and ranging from 120kDa->1MDa. Through single particle EM studies we have now been able to directly visualise the small molecule inhibitor binding site opening up new avaenues for structure based drug design on systems, intractable to other structural techniques (Figure 2).


 Figure 2. Cryo-EM map of an inhibitor-protein complex.

Centre Membership

Integrative Membrane Biology
Structural Molecular Biology


PhD opportunities

Wellcome Trust PhD program http://www.astbury.leeds.ac.uk/join/WT/intro.php

British Heart Foundation PhD studentships http://www.cardiovascular.leeds.ac.uk/opportunities/

BBSRC Doctoral training program http://www.astbury.leeds.ac.uk/join/BBSRC/intro.php





Faculty Research and Innovation

Studentship information

Undergraduate project topics:

  • Developing new inhibitors towards protozoan parasites.
  • Structural studies of membrane protiens

Postgraduate studentship areas:

  • Structural and biochemical analysis of large protein complexes, in particular membrane bound complexes.
  • The development of the time-resolved cryo-EM methodologies
  • Structure based inhibitor design of membrane proteins

See also:

Modules managed

BMSC5301M - Advanced Research Topics

Modules taught

BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5392M - Bioscience MSc Research Project
BIOW5901X - Foundation module
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2224 - Principles of Drug Discovery
BMSC2230 - Topics in Pharmacology
BMSC3140 - Advanced Scientific Skills
BMSC3301 - Research Project in Biomedical Sciences
BMSC5301M - Advanced Research Topics

Centre membership: The Astbury Centre for Structural Molecular Biology

Dr Dimitrios Kontziampasis  (Research Fellow in Cryo-Electron Microscopy)

My main interests revolve around inter/crossdisciplinary and applied research with a focus on surface modification of materials for biological, energy and other applications, and their characterization.  

Mr Tony Palmer  (Visiting Research Fellow)


Simon Davies (Primary supervisor) 50% FTE
Sophie Hesketh (Primary supervisor) 50% FTE
Rachel Johnson (Primary supervisor) 50% FTE
Heather Darby (Co-supervisor) 40% FTE
Brendan Farrell (Co-supervisor) 33% FTE
Jonathan Foster (Co-supervisor) 30% FTE
Faheem Shaik (Co-supervisor) 30% FTE
Craig Wilkinson (Co-supervisor) 50% FTE
Jack Wright (Co-supervisor) 33% FTE

Agip A-NA, Blaza JN, Bridges HR, Viscomi C, Rawson S, Muench SP, Hirst J Cryo-EM structures of complex I from mouse heart mitochondria in two biochemically defined states. Nature structural&molecular biology, 2018
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Drulyte I, Johnson RM, Hesketh EL, Hurdiss DL, Scarff CA, Porav SA, Ranson NA, Muench SP, Thompson RF Approaches to altering particle distributions in cryo-electron microscopy sample preparation Acta Crystallographica Section D: Structural Biology 74 560-571, 2018
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Vasilca V, Sadeghpour A, Rawson S, Hawke LE, Baldwin SA, Wilkinson T, Bannister D, Postis VLG, Rappolt M, Muench SP, Jeuken LJC Spherical-supported membranes as platforms for screening against membrane protein targets Analytical Biochemistry 549 58-65, 2018
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Muench SP, van der Laan M No Longer Hidden Secrets of Proton Pumping: The Resolution Revolution Enlightens V-ATPases Molecular Cell 69 921-922, 2018
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Amporndanai K, Johnson RM, O'Neill PM, Fishwick CWG, Jamson AH, Rawson S, Muench SP, Samar Hasnain S, Antonyuk SV X-ray and cryo-EM structures of inhibitor-bound cytochrome bc 1 complexes for structure-based drug discovery IUCrJ 5 200-210, 2018
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Rawson S, Bisson C, Hurdiss DL, Fazal A, McPhillie MJ, Sedelnikova SE, Baker PJ, Rice DW, Muench SP Elucidating the structural basis for differing enzyme inhibitor potency by cryo-EM Proceedings of the National Academy of Sciences of the United States of America 115 1795-1800, 2018
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Parmar M, Rawson S, Scarff CA, Goldman A, Dafforn TR, Muench SP, Postis VLG Using a SMALP platform to determine a sub-nm single particle cryo-EM membrane protein structure Biochimica et Biophysica Acta - Biomembranes 1860 378-383, 2018
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Harrison MA, Muench SP The vacuolar ATPase– A nano-scale motor that drives cell biology In Subcellular Biochemistry, 2018
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Seneviratne R, Khan S, Moscrop E, Rappolt M, Muench SP, Jeuken LJC, Beales PA A reconstitution method for integral membrane proteins in hybrid lipid-polymer vesicles for enhanced functional durability Methods, 2018
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Berrout J, Kyriakopoulou E, Moparthi L, Hogea AS, Berrout L, Ivan C, Lorger M, Boyle J, Peers C, Muench S, Gomez JE, Hu X, Hurst C, Hall T, Umamaheswaran S, Wesley L, Gagea M, Shires M, Manfield I, Knowles MA, Davies S, Suhling K, Gonzalez YT, Carragher N, Macleod K, Abbott NJ, Calin GA, Gamper N, Zygmunt PM, Timsah Z TRPA1-FGFR2 binding event is a regulatory oncogenic driver modulated by miRNA-142-3p Nature Communications 8, 2017
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Johnson R, Rawson S, McPhillie M, Fishwick C, Muench S The Growing Role of Electron Microscopy in Anti-parasitic Drug Discovery Current Medicinal Chemistry 24, 2017
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Qi W, Manfield IW, Muench SP, Baker A AtSPX1 affects the AtPHR1–DNA-binding equilibrium by binding monomeric AtPHR1 in solution Biochemical Journal 474 3675-3687, 2017
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Muench SP Everyone needs good neighbours– the intricate relationship between the acetylcholine-receptor channel and its membrane environment IUCrJ 4 306-307, 2017

Rawson S, McPhillie MJ, Johnson RM, Fishwick CWG, Muench SP The potential use of single-particle electron microscopy as a tool for structure-based inhibitor design Acta Crystallographica Section D: Structural Biology 73 534-540, 2017
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Caseley EA, Muench SP, Jiang LH Conformational changes during human P2X7 receptor activation examined by structural modelling and cysteine-based cross-linking studies Purinergic Signalling 13 135-141, 2017
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Beales PA, Khan S, Muench SP, Jeuken LJC Durable vesicles for reconstitution of membrane proteins in biotechnology Biochemical Society Transactions 45 15-26, 2017
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Ceasar SA, Baker A, Muench SP, Ignacimuthu S, Baldwin SA The conservation of phosphate-binding residues among PHT1 transporters suggests that distinct transport affinities are unlikely to result from differences in the phosphate-binding site Biochemical Society Transactions 44 1541-1548, 2016
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Caseley EA, Muench SP, Fishwick CW, Jiang LH Structure-based identification and characterisation of structurally novel human P2X7 receptor antagonists Biochemical Pharmacology 116 130-139, 2016
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Rawson S, Iadanza MG, Ranson NA, Muench SP Erratum: Corrigendum to‘Methods to account for movement and flexibility in cryo-EM data processing’ [Methods (2016) 100 (35–41)] Methods 104 207-, 2016
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Lee SC, Knowles TJ, Postis VLG, Jamshad M, Parslow RA, Lin YP, Goldman A, Sridhar P, Overduin M, Muench SP, Dafforn TR A method for detergent-free isolation of membrane proteins in their local lipid environment Nature Protocols 11 1149-1162, 2016
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Palmer AJ, Baker A, Muench SP The varied functions of aluminium-activated malate transporters–much more than aluminium resistance Biochemical Society Transactions 44 856-862, 2016
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Qi W, Baldwin SA, Muench SP, Baker A P<inf>i</inf>sensing and signalling: From prokaryotic to eukaryotic cells Biochemical Society Transactions 44 766-773, 2016
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Rawson S, Harrison MA, Muench SP Rotating with the brakes on and other unresolved features of the vacuolar ATPase Biochemical Society Transactions 44 851-855, 2016
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McPhillie M, Zhou Y, El Bissati K, Dubey J, Lorenzi H, Capper M, Lukens AK, Hickman M, Muench S, Verma SK, Weber CR, Wheeler K, Gordon J, Sanders J, Moulton H, Wang K, Kim T-K, He Y, Santos T, Woods S, Lee P, Donkin D, Kim E, Fraczek L, Lykins J, Esaa F, Alibana-Clouser F, Dovgin S, Weiss L, Brasseur G, Wirth D, Kent M, Hood L, Meunieur B, Roberts CW, Hasnain SS, Antonyuk SV, Fishwick C, McLeod R New paradigms for understanding and step changes in treating active and chronic, persistent apicomplexan infections. Sci Rep 6 29179-, 2016
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Papachristos K, Muench SP, Paci E Characterization of the flexibility of the peripheral stalk of prokaryotic rotary A-ATPases by atomistic simulations. Proteins 84 1203-1212, 2016
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Muench SP Guest Editor’s Introduction: Single Particle Cryo-EM, from sample to reconstruction Methods 100 1-2, 2016

Rawson S, Iadanza MG, Ranson NA, Muench SP Methods to account for movement and flexibility in cryo-EM data processing Methods 100 35-41, 2016
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Thompson RF, Walker M, Siebert CA, Muench SP, Ranson NA An introduction to sample preparation and imaging by cryo-electron microscopy for structural biology Methods 100 3-15, 2016
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Smith GA, Howell GJ, Phillips C, Muench SP, Ponnambalam S, Harrison MA Extracellular and luminal pH regulation by vacuolar H<sup>+</sup>-ATPase isoform expression and targeting to the plasma membrane and endosomes Journal of Biological Chemistry 291 8500-8515, 2016
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Solernou A, Read D, Harris S, Harlen O, Smith K, Muench S Bottom-up approach to describe protein-protein interactions in a continuum mechanics biomolecular model of fibrin, 2016

Rawson S, Davies S, Lippiat JD, Muench SP The changing landscape of membrane protein structural biology through developments in electron microscopy Molecular Membrane Biology 33 12-22, 2016
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Khan S, Li M, Muench SP, Jeuken LJC, Beales PA Durable proteo-hybrid vesicles for the extended functional lifetime of membrane proteins in bionanotechnology Chemical Communications 52 11020-11023, 2016
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Bain J, Ruiz-Pérez L, Kennerley AJ, Muench SP, Thompson R, Battaglia G, Staniland SS In situ formation of magnetopolymersomes via electroporation for MRI Scientific Reports 5, 2015
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Wrighton DC, Muench SP, Lippiat JD Mechanism of inhibition of mouse Slo3 (K&lt;inf&gt;Ca&lt;/inf&gt;5.1) potassium channels by quinine, quinidine and barium British Journal of Pharmacology 172 4355-4363, 2015
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Caseley EA, Muench SP, Baldwin SA, Simmons K, Fishwick CW, Jiang LH Docking of competitive inhibitors to the P2X7 receptor family reveals key differences responsible for changes in response between rat and human Bioorganic and Medicinal Chemistry Letters 25 3164-3167, 2015
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Rawson S, Phillips C, Huss M, Tiburcy F, Wieczorek H, Trinick J, Harrison MA, Muench SP Structure of the vacuolar H<sup>+</sup>-ATPase rotary motor reveals new mechanistic insights Structure 23 461-471, 2015
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Postis V, Rawson S, Mitchell JK, Lee SC, Parslow RA, Dafforn TR, Baldwin SA, Muench SP The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy Biochimica et Biophysica Acta - Biomembranes 1848 496-501, 2015
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Henriquez FL, Campbell SJ, Sundararaj BK, Cano A, Muench SP, Roberts CW The Acanthamoeba Shikimate Pathway has a Unique Molecular Arrangement and is Essential for Aromatic Amino Acid Biosynthesis Protist 166 93-105, 2015
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Magee T, Henderson P, Baker A, Postis V, Muench S A Tribute to Stephen Allan Baldwin Molecular membrane biology 32 33-34, 2015

Baker A, Ceasar SA, Palmer AJ, Paterson JB, Qi W, Muench SP, Baldwin SA Replace, reuse, recycle: Improving the sustainable use of phosphorus by plants Journal of Experimental Botany 66 3523-3540, 2015
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Caseley EA, Muench SP, Roger S, Mao H-J, Baldwin SA, Jiang L-H Non-synonymous single nucleotide polymorphisms in the P2X receptor genes: association with diseases, impact on receptor functions and potential use as diagnosis biomarkers. Int J Mol Sci 15 13344-13371, 2014
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Witola WH, Liu SR, Montpetit A, Welti R, Hypolite M, Roth M, Zhou Y, Mui E, Cesbron-Delauw M-F, Fournie GJ, Cavailles P, Bisanz C, Boyer K, Withers S, Noble AG, Swisher CN, Heydemann PT, Rabiah P, Muench SP, McLeod R ALOX12 in human toxoplasmosis. Infect Immun 82 2670-2679, 2014
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Muench SP, Rawson S, Eyraud V, Delmas AF, Da Silva P, Phillips C, Trinick J, Harrison MA, Gressent F, Huss M PA1b inhibitor binding to subunits c and e of the vacuolar ATPase reveals its insecticidal mechanism. J Biol Chem 289 16399-16408, 2014
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Wilkinson C, McPhillie MJ, Zhou Y, Woods S, Afanador GA, Rawson S, Khaliq F, Prigge ST, Roberts CW, Rice DW, McLeod R, Fishwick CW, Muench SP The benzimidazole based drugs show good activity against T. gondii but poor activity against its proposed enoyl reductase enzyme target. Bioorg Med Chem Lett 24 911-916, 2014
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Muench SP, Scheres SHW, Huss M, Phillips C, Vitavska O, Wieczorek H, Trinick J, Harrison MA Subunit positioning and stator filament stiffness in regulation and power transmission in the V1 motor of the Manduca sexta V-ATPase. J Mol Biol 426 286-300, 2014
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Richardson RA, Papachristos K, Read DJ, Harlen OG, Harrison M, Paci E, Muench SP, Harris SA Understanding the apparent stator-rotor connections in the rotary ATPase family using coarse-grained computer modeling Proteins: Structure, Function and Bioinformatics 82 3298-3311, 2014
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Zhou Y, Fomovska A, Muench S, Lai B-S, Mui E, McLeod R Spiroindolone that inhibits PfATPase4 is a potent, cidal inhibitor of Toxoplasma gondii tachyzoites in vitro and in vivo. Antimicrob Agents Chemother 58 1789-1792, 2014
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Afanador GA, Muench SP, McPhillie M, Fomovska A, Schön A, Zhou Y, Cheng G, Stec J, Freundlich JS, Shieh H-M, Anderson JW, Jacobus DP, Fidock DA, Kozikowski AP, Fishwick CW, Rice DW, Freire E, McLeod R, Prigge ST Discrimination of potent inhibitors of Toxoplasma gondii enoyl-acyl carrier protein reductase by a thermal shift assay. Biochemistry 52 9155-9166, 2013
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Song CF, Papachristos K, Rawson S, Huss M, Wieczorek H, Paci E, Trinick J, Harrison MA, Muench SP Flexibility within the Rotor and Stators of the Vacuolar H+-ATPase PLOS ONE 8, 2013

Muench SP, Stec J, Zhou Y, Afanador GA, McPhillie MJ, Hickman MR, Lee PJ, Leed SE, Auschwitz JM, Prigge ST, Rice DW, McLeod R Development of a triclosan scaffold which allows for adaptations on both the A- and B-ring for transport peptides BIOORGANIC&MEDICINAL CHEMISTRY LETTERS 23 3551-3555, 2013

Cheng G, Muench SP, Zhou Y, Afanador GA, Mui EJ, Fomovska A, Lai BS, Prigge ST, Woods S, Roberts CW, Hickman MR, Lee PJ, Leed SE, Auschwitz JM, Rice DW, McLeod R Design, synthesis, and biological activity of diaryl ether inhibitors of Toxoplasma gondii enoyl reductase Bioorganic and Medicinal Chemistry Letters 23 2035-2043, 2013
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Cheng G, Muench SP, Zhou Y, Mui EJ, Fomovska A, Lai BS, McLeod R, Afanador GA, Prigge ST, Woods S, Roberts CW, Rice DW, Hickman MR, Lee PJ, Leed SE, Auschwitz JM Design, synthesis, and biological activity of diaryl ether inhibitors of Toxoplasma gondii enoyl reductase Bioorganic and Medicinal Chemistry Letters, 2013
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Stec J, Kozikowski AP, Fomovska A, Zhou Y, Lai B-S, ElBissati K, Mcleod R, Afanador GA, Prigge ST, Muench SP, Hickman MR, Lee PJ, Leed SE, Auschwitz JM, Sommervile C, Woods S, Roberts CW, Rice D Modification of Triclosan Scaffold in Search of Improved Inhibitors for Enoyl-Acyl Carrier Protein (ACP) Reductase in Toxoplasma gondii ChemMedChem, 2013
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Fomovska A, Huang Q, El Bissati K, Mui EJ, Witola WH, Cheng G, Zhou Y, Sommerville C, Roberts CW, Bettis S, Prigge ST, Afanador GA, Hickman MR, Lee PJ, Leed SE, Auschwitz JM, Pieroni M, Stec J, Muench SP, Rice DW, Kozikowski AP, McLeod R Novel N-Benzoyl-2-Hydroxybenzamide Disrupts Unique Parasite Secretory Pathway ANTIMICROBIAL AGENTS AND CHEMOTHERAPY 56 2666-2682, 2012

Muench SP, Trinick J, Harrison MA Structural divergence of the rotary ATPases Q REV BIOPHYS 44 311-356, 2011

Bockelmann S, Menche D, Rudolph S, Bender T, Grond S, von Zezschwitz P, Muench SP, Wieczorek H, Huss M Archazolid A Binds to the Equatorial Region of the c-Ring of the Vacuolar H+-ATPase J BIOL CHEM 285 38304-38314, 2010

Hutson SL, Mui E, Kinsley K, Witola WH, Behnke MS, El Bissati K, Muench SP, Rohrman B, Liu SR, Wollmann R, Ogata Y, Sarkeshik A, Yates JR, McLeod R T. gondii RP Promoters&Knockdown Reveal Molecular Pathways Associated with Proliferation and Cell-Cycle Arrest PLOS ONE 5, 2010

Tipparaju SK, Muench SP, Mui EJ, Ruzheinikov SN, Lu JZ, Hutson SL, Kirisits MJ, Prigge ST, Roberts CW, Henriquez FL, Kozikowski AP, Rice DW, McLeod RL Identification and Development of Novel Inhibitors of Toxoplasma gondii Enoyl Reductase J MED CHEM 53 6287-6300, 2010

Tan TG, Mui E, Cong H, Witola WH, Montpetit A, Muench SP, Sidney J, Alexander J, Sette A, Grigg ME, Maewal A, McLeod R Identification of T-gondii epitopes, adjuvants, and host genetic factors that influence protection of mice and humans VACCINE 28 3977-3989, 2010

Muench SP, Huss M, Song CF, Phillips C, Wieczorek H, Trinick J, Harrison MA Cryo-electron Microscopy of the Vacuolar ATPase Motor Reveals its Mechanical and Regulatory Complexity J MOL BIOL 386 989-999, 2009

Muench SP, Huss M, Phillips C, Wieczorek H, Trinick J, Harrison M A Molecular Gearbox: The Mechanical And Regulatory Complexity Of The Vacuolar ATPase Revealed, 2009

Henriquez FL, Ingram PR, Muench SP, Rice DW, Roberts CW Molecular basis for resistance of Acanthamoeba tubulins to all major classes of antitubulin compounds ANTIMICROB AGENTS CH 52 1133-1135, 2008

Mui EJ, Schiehser GA, Milhous WK, Hsu HH, Roberts CW, Kirisits M, Muench S, Rice D, Dubey JP, Fowble JW, Rathod PK, Queener SF, Liu SR, Jacobus DP, McLeod R Novel Triazine JPC-2067-B Inhibits Toxoplasma gondii In Vitro and In Vivo PLOS NEGLECT TROP D 2, 2008

Lu JZ, Muench SP, Allary M, Campbell S, Roberts CW, Mui E, Mcleod RL, Rice DW, Prigge ST Type I and type II fatty acid biosynthesis in Eimeria tenella: enoyl reductase activity and structure PARASITOLOGY 134 1949-1962, 2007

Muench SP, Prigge ST, McLeod R, Rafferty JB, Kirisits MJ, Roberts CW, Mui EJ, Rice DW Studies of Toxoplasma gondii and Plasmodium falciparum enoyl acyl carrier protein reductase and implications for the development of antiparasitic agents ACTA CRYSTALLOGR D 63 328-338, 2007

Ferguson DJP, Campbell SA, Henriquez FL, Phan L, Mui E, Richards TA, Muench SP, Allary M, Lu JZ, Prigge ST, Tomley F, Shirley MW, Rice DW, McLeod R, Roberts CW Enzymes of type II fatty acid synthesis and apicoplast differentiation and division in Eimeria tenella INT J PARASITOL 37 33-51, 2007

Muench SP, Xu L, Sedelnikova SE, Rice DW The essential GTPase YphC displays a major domain rearrangement associated with nucleotide binding P NATL ACAD SCI USA 103 12359-12364, 2006

Muench SP, Prigge ST, Zhu LQ, Kirisits MJ, Roberts CW, Wernimont S, McLeod R, Rice DW Expression, purification and preliminary crystallographic analysis of the Toxoplasma gondii enoyl reductase ACTA CRYSTALLOGR F 62 604-606, 2006

Xu L, Muench SP, Roujeinikova A, Sedelnikova SE, Rice DW Cloning, purification and preliminary crystallographic analysis of the Bacillus subtilis GTPase YphC-GDP complex Acta Crystallographica Section F: Structural Biology and Crystallization Communications 62 435-437, 2006
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Ferguson DJP, Henriquez FL, Kirisits MJ, Muench SP, Prigge ST, Rice DW, Roberts CW, McLeod RL Maternal inheritance and stage-specific variation of the apicoplast in Toxoplasma gondii during development in the intermediate and definitive host EUKARYOT CELL 4 814-826, 2005

Muench S Structural studies of enoyl acyl carrier protein reductase from Plasmodium falciparum and Toxoplasma gondii., 2004

Samuel BU, Hearn B, Mack D, Wender P, Rothbard J, Kirisits MJ, Mui E, Wernimont S, Roberts CW, Muench SP, Rice DW, Prigge ST, Law AB, McLeod R Delivery of antimicrobials into parasites P NATL ACAD SCI USA 100 14281-14286, 2003

Muench SP, Rafferty JB, Mcleod R, Rice DW, Prigge ST Expression, purification and crystallization of the Plasmodium falciparum enoyl reductase ACTA CRYSTALLOGR D 59 1246-1248, 2003

Aunpad R, Muench SP, Baker PJ, Sedelnikova S, Panbangred W, Doukyu N, Aono R, Rice DW Crystallization and preliminary X-ray crystallographic studies on the class II cholesterol oxidase from Burkholderia cepacia containing bound flavin ACTA CRYSTALLOGR D 58 2182-2183, 2002

McLeod R, Muench SP, Rafferty JB, Kyle DE, Mui EJ, Kirisits MJ, Mack DG, Roberts CW, Samuel BU, Lyons RE, Dorris M, Milhous WK, Rice DW Triclosan inhibits the growth of Plasmodium falciparum and Toxoplasma gondii by inhibition of apicomplexan Fab I INT J PARASITOL 31 109-113, 2001

Matherly LH, Muench SP Evidence for a localized conversion of endogenous tetrahydrofolate cofactors to dihydrofolate as an important element in antifolate action in murine leukemia cells Biochemical Pharmacology 39 2005-2014, 1990
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Matherly LH, Czajkowski CA, Muench SP, Psiakis JT Role for Cytosolic Folate-binding Proteins in the Compartmentation of Endogenous Tetrahydrofolates and the 5-Formyl Tetrahydrofolate-mediated Enhancement of 5-Fluoro-2′-deoxyuridine Antitumor Activity in Vitro Cancer Research 50 3262-3269, 1990
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Parmar MJ, Lousa CDM, Muench SP, Goldman A, Postis VLG Artificial membranes for membrane protein purification, functionality and structure studies. Biochem Soc Trans 44 877-882
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