Prof Paul Millner

BSc, PhD Feb 1979, Leeds
Professor in Bionanotechnology
School of Biomedical Sciences

Background: Postdoctoral work at Purdue University, Indiana USA and Imperial College, London. Appointed Lecturer 1986, Senior Lecturer 1993, Reader 2006.

Contact: Garstang 6.44f, +44(0) 113 34 33149, email address for  

You can read more about Prof Millner's interests here:
www.fbs.leeds.ac.uk/lab/Millner_P/
www.colorectal.htc.nihr.ac.uk

Research Interests

Nano-scale enginering of surfaces for biosensor applications, nanoparticulate supports, bio-affinity systems

Reagentless Electrochemical Biosensors:

an immunosensor

We have developed a new reagentless immunosensor platform which allows highly sensitive single step measurement of a wide range of substances. Biosensors developed include those for GM-material in food stuffs (EC Project IMAGEMO), based on affinity immobilisation of tagged antibodies to self assembled mixed monolayers (Fig. 1) or to electropolymerised polyaniline layers (EC Project ELISHA - see www.immunosensors.com). Immunosensors for antibiotics, herbicides, cardiac, cancer and neurodegenerative disease markers have been developed, whilst immunosensors for viruses are under development. We are using other binding proteins to create biosensors for chitin (fungal infection marker) and low Mw hydrophobic molecules. In particular, we are interested in the nanoscale structure of the sensor surface and how this influences the impedance signals observed.

 

biosilicate nanoparticles

Nanoparticle and microparticles:
Work is ongoing to develop nanoparticles of various compositions as enzyme supports for biocatalysis and for applications in biosensing. Biosilicate nanoparticles of defined dimensions and biosilicate surface coating have been produced which can entrap and/or stabilise commercially interesting enzymes (EC Project SANTS - www.sants-nanosilicates.com). An scanning EM image of the nanoparticles is shown in Fig. 2.

We have also developed polymeric nanoparticles within project EC Project COMBIO; these have 'tunable' properties including user defined size and a range of surface chemistries to allow attachment of other biomolecules and aggregation by simple manipulation of pH or ionic environment. Magnetic nanoparticles have also been developed.

We have also developed microparticles based on carageenan, a natural biodegradeable polysaccharide isolated from red seaweeds which provide cheap, biodegradeable and disposable supports for the attachment of enzymes for biocatalysis.

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Fast electrical interrogation of biosensors
    Keywords: Biosensor, electrode, quartz crystal microbalance (QCM), nanotechnology (Laboratory)
  • Application of functionalised nanoparticles for biosensing and intraoperative cancer imaging
    Keywords: nanoparticle synthesis, size measurement, antibody, nanotechnology (Laboratory)
  • Fabrication of photosensitizer loaded nanostructured surfaces for microbial and chemical water pollutant cleanup.
    Keywords: electrospun nanofibres, metal oxide nanorods, photosensitizers, nanotechnology (Laboratory)

Postgraduate studentship areas:

  • Enzyme-based biosensors and immunosensors for medical, food and envronmental analyses
  • Molecular mechanisms underlying impedance biosensor signal generation
  • Application of functionalised nanoparticles for biosensing and cancer imaging
  • fabrication of very high area surfaces and their application to photosensitized water cleanup

See also:

Modules taught

BIOL5269M - Commercialising Biopharmaceutical Products
BIOL5276M - Medical Diagnostics
BIOL5280M - Integrated Drug Development Plan
BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5372M - Advanced Biomolecular Technologies
BIOL5392M - Bioscience MSc Research Project
BMSC1103 - Basic Laboratory and Scientific Skills
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2233 - Topics in Medical Sciences
BMSC3140 - Advanced Scientific Skills
BMSC3238 - Biomedical Nanotechnology
BMSC3301 - Research Project in Biomedical Sciences

Centre membership: The Centre for Molecular Nanoscience

Group Leader Prof Paul Millner  (Professor in Bionanotechnology)

Nano-scale enginering of surfaces for biosensor applications, nanoparticulate supports, bio-affinity systems 

Dr Timothy Gibson  (Visiting Research Administrator)

Research Interests and Practical Exploitation of Research:
Biosensors and Chemical Sensor Technology, Electronic Nose Technology, Molecular Interactions at Sensor Surfaces and Stabilisation of Proteins, Enzymes and Antibodies.
 

Mr Jack Goode  (Research Fellow in Optical Nanobiosensors for Infection Biomarkers)

Dr Lewis Mackenzie  (Visiting Research Fellow)

Biomedical optics, nano-sensor development, blood diagnostics, in vivo oximetry 

Dr Alexandre Vakurov  (Research Fellow)


Postgraduates

Eiman Alenezi (Primary supervisor) 60% FTE
Kaniz Chowdhury (Primary supervisor) 75% FTE
Juan Leva (Primary supervisor) 50% FTE
Thanisorn Mahatnirunkul (Primary supervisor) 50% FTE
Hussaini Majiya (Primary supervisor) 75% FTE
Shazana Hilda Binti Shamsuddin (Primary supervisor) 50% FTE
Pattanapong Thangsunan (Primary supervisor) 50% FTE
Meaad Almusined (Co-supervisor) 10% FTE
Fayez Alsulaimani (Co-supervisor) 40% FTE
Beatriz Álvarez Rodriguez (Co-supervisor) 33% FTE
Andrew Brown (Co-supervisor) 10% FTE
Yazan Sulaiman Khaled (Co-supervisor) 33% FTE
Forough Torabi Baghkomeh (Co-supervisor) 50% FTE

Mackenzie LE, Goode JA, Vakurov A, Nampi PP, Saha S, Jose G, Millner PA The theoretical molecular weight of NaYF<inf>4</inf>:RE upconversion nanoparticles Scientific Reports 8, 2018
DOI:10.1038/s41598-018-19415-w
View abstract

Khaled YS, Shamsuddin S, Tiernan J, McPherson M, Hughes T, Millner P, Jayne DG THERANOSTIC FOSLIP LOADED AND CEA-AFFIMER FUNCTIONALISED SILICA NANOPARTICLES FOR FLUORESCENT IMAGING AND PHOTODYNAMIC THERAPY OF COLORECTAL CANCER, 2018

Majiya H, Adeyemi OO, Stonehouse NJ, Millner P Photodynamic inactivation of bacteriophage MS2: The A-protein is the target of virus inactivation Journal of Photochemistry and Photobiology B: Biology 178 404-411, 2018
DOI:10.1016/j.jphotobiol.2017.11.032
View abstract

Hazra S, Joshi H, Ghosh BK, Ahmed A, Gibson T, Millner P, Ghosh NN Development of a Ru Nanoparticle Loaded Thiol Functionalized Meso Porous Silica Modified Screen Printed Au Electrode for Electrochemical Detection and Estimation of Glucose Journal of Nanoscience and Nanotechnology 17 1163-1170, 2017
DOI:10.1166/jnn.2017.12714
View abstract

Majiya H, Chowdhury KF, Stonehouse NJ, Millner P TMPyP functionalised chitosan membrane for efficient sunlight driven water disinfection Journal of Water Process Engineering, 2017
DOI:10.1016/j.jwpe.2017.08.013
View abstract

Goode J, Dillon G, Millner PA The development and optimisation of Nanobody based electrochemical immunosensors for IgG Sensors and Actuators B: Chemical 234 478-484, 2016
DOI:10.1016/j.snb.2016.04.132
View abstract

Makaraviciute A, Jackson CD, Millner PA, Ramanaviciene A Considerations in producing preferentially reduced half-antibody fragments Journal of Immunological Methods 429 50-56, 2016
DOI:10.1016/j.jim.2016.01.001
View abstract

Nampi PP, Varma H, Biju PR, Kakkar T, Jose G, Saha S, Millner P Sodium yttrium fluoride based upconversion nano phosphors for biosensing, 2015
DOI:10.1088/1742-6596/619/1/012043
View abstract

Tiernan JP, Ingram N, Marston G, Perry SL, Rushworth JV, Coletta PL, Millner PA, Jayne DG, Hughes TA CEA-targeted nanoparticles allow specific in vivo fluorescent imaging of colorectal cancer models Nanomedicine 10 1223-1231, 2015
DOI:10.2217/nnm.14.202
View abstract

Hyde EDE, Moreno-Atanasio R, Millner PA, Neville F Surface charge control through the reversible adsorption of a biomimetic polymer on silica particles Journal of Physical Chemistry B 119 1726-1735, 2015
DOI:10.1021/jp5100439
View abstract

Hazra S, Joshi H, Ghosh BK, Ahmed A, Gibson T, Millner P, Ghosh NN Development of a novel and efficient H&lt;inf&gt;2&lt;/inf&gt;O&lt;inf&gt;2&lt;/inf&gt; sensor by simple modification of a screen printed Au electrode with Ru nanoparticle loaded functionalized mesoporous SBA15 RSC Advances 5 34390-34397, 2015
DOI:10.1039/c5ra02712h
View abstract

Goode JA, Rushworth JV, Millner PA Biosensor Regeneration: A Review of Common Techniques and Outcomes. Langmuir 31 6267-6276, 2014
DOI:10.1021/la503533g
View abstract

Ahmed A, Rushworth JV, Hirst NA, Millner PA Biosensors for whole-cell bacterial detection. Clin Microbiol Rev 27 631-646, 2014
DOI:10.1128/CMR.00120-13
View abstract

Rushworth JV, Ahmed A, Griffiths HH, Pollock NM, Hooper NM, Millner PA A label-free electrical impedimetric biosensor for the specific detection of Alzheimer's amyloid-beta oligomers. Biosens Bioelectron 56 83-90, 2014
DOI:10.1016/j.bios.2013.12.036
View abstract

Hirst NA, Tiernan JP, Millner PA, Jayne DG Systematic review of methods to predict and detect anastomotic leakage in colorectal surgery. Colorectal Dis 16 95-109, 2014
DOI:10.1111/codi.12411
View abstract

Goode JA, Ahmed A, Pike D, Millner PA, Kapur N, Stewart D Comparative Electrochemical Analysis of Two Flow Cell Systems JOURNAL OF THE ELECTROCHEMICAL SOCIETY 161 B143-B146, 2014
DOI:10.1149/2.073406jes

Ahmed A, Rushworth JV, Wright JD, Millner PA Novel Impedimetric Irnmunosensor for Detection of Pathogenic Bacteria Streptococcus pyogenes in Human Saliva ANALYTICAL CHEMISTRY 85 12118-12125, 2013
DOI:10.1021/ac403253j

Hirst NA, Hazelwood LD, Jayne DG, Millner PA An amperometric lactate biosensor using H2O2 reduction via a Prussian Blue impregnated poly(ethyleneimine) surface on screen printed carbon electrodes to detect anastomotic leak and sepsis SENSORS AND ACTUATORS B-CHEMICAL 186 674-680, 2013
DOI:10.1016/j.snb.2013.06.090

Charpentier TVJ, Neville A, Millner P, Hewson RW, Morina A Development of anti-icing materials by chemical tailoring of hydrophobic textured metallic surfaces Journal of Colloid and Interface Science 394 539-544, 2013
DOI:10.1016/j.jcis.2012.11.021
View abstract

Pike DJ, Kapur N, Millner PA, Stewart DI Flow cell design for effective biosensing. Sensors (Basel) 13 58-70, 2012
DOI:10.3390/s130100058
View abstract

Vakurov A, Pchelintsev NA, Gibson T, Millner P Development of polymeric nanoparticles showing tuneable pH-responsive precipitation Journal of Nanoparticle Research 14, 2012
DOI:10.1007/s11051-012-1302-x
View abstract

Billah MM, Hays HCW, Hodges CS, Ponnambalam S, Vohra R, Millner PA Mixed self-assembled monolayer (mSAM) based impedimetric immunosensors for cardiac troponin I (cTnI) and soluble lectin-like oxidized low-density lipoprotein receptor-1 (sLOX-1) SENSORS AND ACTUATORS B-CHEMICAL 173 361-366, 2012
DOI:10.1016/j.snb.2012.07.017

Tiernan JP, Ansari I, Hirst NA, Millner PA, Hughes TA, Jayne DG Intra-operative tumour detection and staging in colorectal cancer surgery. Colorectal Dis 14 e510-e520, 2012
DOI:10.1111/j.1463-1318.2012.03078.x
View abstract

Millner PA, Caygill RL, Conroy DJR, Shahidan MA Impedance interrogated affinity biosensors for medical applications: Novel targets and mechanistic studies, 2012
DOI:10.1016/B978-1-84569-935-2.50004-8
View abstract

Caygill RL, Hodges CS, Holmes JL, Higson SPJ, Blair GE, Millner PA Novel impedimetric immunosensor for the detection and quantitation of Adenovirus using reduced antibody fragments immobilized onto a conducting copolymer surface. Biosens Bioelectron 32 104-110, 2012
DOI:10.1016/j.bios.2011.11.041
View abstract

Neville F, Millner P Fabrication and characterization of bioactive thiol-silicate nanoparticles., 2011
DOI:10.1007/978-1-61779-132-1_11
View abstract

Conroy DJR, Millner P Untitled BIOANALYSIS 3 1439-1440, 2011
DOI:10.4155/BIO.11.139

Pchelintsev NA, Vakurov A, Hays HH, Millner PA Thiols deposition onto the surface of glassy carbon electrodes mediated by electrical potential ELECTROCHIM ACTA 56 2696-2702, 2011
DOI:10.1016/j.electacta.2010.12.043

Neville F, Broderick MJF, Gibson T, Millner PA Fabrication and Activity of Silicate Nanoparticles and Nanosilicate-Entrapped Enzymes Using Polyethyleneimine As a Biomimetic Polymer LANGMUIR 27 279-285, 2011
DOI:10.1021/la1033492

Caygill RL, Blair GE, Millner PA A review on viral biosensors to detect human pathogens ANAL CHIM ACTA 681 8-15, 2010
DOI:10.1016/j.aca.2010.09.038

Billah MM, Hodges CS, Hays HCW, Millner PA Directed immobilization of reduced antibody fragments onto a novel SAM on gold for myoglobin impedance immunosensing BIOELECTROCHEMISTRY 80 49-54, 2010
DOI:10.1016/j.bioelechem.2010.08.005

Forde J, Tully E, Vakurov A, Gibson TD, Millner P, O'Fagain C Chemical modification and immobilisation of laccase from Trametes hirsuta and from Myceliophtora thermophila (vol 46, pg 430, 2010) ENZYME AND MICROBIAL TECHNOLOGY 47 241-241, 2010
DOI:10.1016/j.enzmictec.2010.07.002

Forde J, Tully E, Vakurov A, Gibson TD, Millner P,Ó'Fágáin C Corrigendum to Chemical modification and immobilisation of laccase from Trametes hirsuta and from Myceliophtora thermophila Enzyme and Microbial Technology 47 241-, 2010
DOI:10.1016/j.enzmictec.2010.07.002

Rodgers MA, Findlay JBC, Millner PA Lipocalin based biosensors for low mass hydrophobic analytes; development of a novel SAM for polyhistidine tagged proteins SENSOR ACTUAT B-CHEM 150 12-18, 2010
DOI:10.1016/j.snb.2010.07.053

Forde J, Vakurov A, Gibson TD, Millner P, Whelehan M, Marison IW,Ó'Fágáin C Chemical modification and immobilisation of lipase B from Candida antarctica onto mesoporous silicates Journal of Molecular Catalysis B: Enzymatic 66 203-209, 2010
DOI:10.1016/j.molcatb.2010.05.010
View abstract

Forde J, Tully E, Vakurov A, Gibson TD, Millner P,Ó'Fágáin C Chemical modification and immobilisation of laccase from Trametes hirsuta and from Myceliophthora thermophila Enzyme and Microbial Technology 46 430-437, 2010
DOI:10.1016/j.enzmictec.2010.01.004
View abstract

Conroy DJR, Millner PA, Stewart DI, Pollmann K Biosensing for the Environment and Defence: Aqueous Uranyl Detection Using Bacterial Surface Layer Proteins SENSORS-BASEL 10 4739-4755, 2010
DOI:10.3390/s100504739

Coldrick Z, Steenson P, Millner P, Davies M, Nelson A Phospholipid monolayer coated microfabricated electrodes to model the interaction of molecules with biomembranes, 2009
DOI:10.1016/j.electacta.2009.02.095
View abstract

Vakurov A, Pchelintsev NA, Forde J, 'Fgin C, Gibson T, Millner P The preparation of size-controlled functionalized polymeric nanoparticles in micelles Nanotechnology 20, 2009
DOI:10.1088/0957-4484/20/29/295605
View abstract

Pchelintsev NA, Vakurov A, Millner PA Simultaneous deposition of Prussian Blue and creation of an electrostatic surface for rapid biosensor construction SENSOR ACTUAT B-CHEM 138 461-466, 2009
DOI:10.1016/j.snb.2009.02.039

Neville F, Pchelintsev NA, Broderick MJF, Gibson T, Millner PA Novel one-pot synthesis and characterization of bioactive thiol-silicate nanoparticles for biocatalytic and biosensor applications. Nanotechnology 20 055612-, 2009
DOI:10.1088/0957-4484/20/5/055612
View abstract

Millner PA, Hays HCW, Vakurov A, Pchelintsev NA, Billah MM, Rodgers MA Nanostructured transducer surfaces for electrochemical biosensor construction-Interfacing the sensing component with the electrode SEMIN CELL DEV BIOL 20 34-40, 2009
DOI:10.1016/j.semcdb.2009.01.011

Barton AC, Collyer SD, Davis F, Garifallou GZ, Tsekenis G, Tully E, O'Kennedy R, Gibson T, Millner PA, Higson SPJ Labeless AC impedimetric antibody-based sensors with pg ml(-1) sensitivities for point-of-care biomedical applications, 2009
DOI:10.1016/j.bios.2008.06.001

Pchelintsev NA, Neville F, Millner PA Biomimetic silication of surfaces and its application to preventing leaching of electrostatically immobilized enzymes SENSOR ACTUAT B-CHEM 135 21-26, 2008
DOI:10.1016/j.snb.2008.07.015

Tsekenis G, Garifallou GZ, Davis F, Millner PA, Pinacho DG, Sanchez-Baeza F, Marco MP, Gibson TD, Higson SPJ Detection of Fluoroquinolone Antibiotics in Milk via a Labeless Immunoassay Based upon an Alternating Current Impedance Protocol ANAL CHEM 80 9233-9239, 2008
DOI:10.1021/ac8014752

Meller Harel HY, Fontaine V, Chen H, Jones IM, Millner PA Display of a maize cDNA library on baculovirus infected insect cells. BMC Biotechnol 8 64-, 2008
DOI:10.1186/1472-6750-8-64
View abstract

Pchelintsev NA, Millner PA A novel procedure for rapid surface functionalisation and mediator loading of screen-printed carbon electrodes ANAL CHIM ACTA 612 190-197, 2008
DOI:10.1016/j.aca.2008.02.022

Tsekenis G, Garifallou GZ, Davis F, Millner PA, Gibson TD, Higson SPJ Label-less immunosensor assay for myelin basic protein based upon an ac impedance protocol ANAL CHEM 80 2058-2062, 2008
DOI:10.1021/ac702070e

Billah M, Hays HCW, Millner PA Development of a myoglobin impedimetric immunosensor based on mixed self-assembled monolayer onto gold MICROCHIMICA ACTA 160 447-454, 2008
DOI:10.1007/s00604-007-0793-0

Davis F, Law KA, Chaniotakis NA, Fournier D, Gibson T, Millner P, Marty JL, Sheehan MA, Ogurtsov VI, Johnson G, Griffiths J, Turner APF, Higson SPJ Chapter 15 Ultra-sensitive determination of pesticides via cholinesterase-based sensors for environmental analysis, 2007
DOI:10.1016/S0166-526X(06)49015-2

Neville F, Broderick M, Gibson T, Millner P Synthesis and activity of nanosilicates and nanosilicate-entrapped enzymes using biomimetic polymers, 2007
DOI:10.1016/j.jbiotec.2007.07.173

Neville F, Broderick M, Gibson T, Millner P Synthesis and activity of nanosilicates and nanosilicate-entrapped enzymes using biomimetic polymers, 2007
DOI:10.1016/j.jbiotec.2007.07.173

Millner P, Vakurov A, Hays H, Pchelintsev N, Billah M, Rodgers M, Gibson T The nanoscale design of surfaces for building biosensors, 2007
DOI:10.1016/j.jbiotec.2007.07.223

Millner P, Vakurov A, Hays H, Pchelintsev N, Billah M, Rodgers M, Gibson T The nanoscale design of surfaces for building biosensors, 2007
DOI:10.1016/j.jbiotec.2007.07.223

Millner P, Broderick M, Neville F, Vakurov A, Gibson T Biosilicate and polymeric nanoparticulate solutions for biocatalysis, 2007
DOI:10.1016/j.jbiotec.2007.07.021

Millner P, Broderick M, Neville F, Vakurov A, Gibson T Biosilicate and polymeric nanoparticulate solutions for biocatalysis, 2007
DOI:10.1016/j.jbiotec.2007.07.021

Vakurov A, Gibson T, Millner P Application of polymeric nanoparticles for biocatalysis, 2007
DOI:10.1016/j.jbiotec.2007.07.022

Vakurov A, Gibson T, Millner P Application of polymeric nanoparticles for biocatalysis, 2007
DOI:10.1016/j.jbiotec.2007.07.022

Broderick M, Neville F, Gibson T, Millner P Practical nanobiotechnology: Functional nanoparticle production using silaffin R5 peptide, 2007
DOI:10.1016/j.jbiotec.2007.07.020

Broderick M, Neville F, Gibson T, Millner P Practical nanobiotechnology: Functional nanoparticle production using silaffin R5 peptide, 2007
DOI:10.1016/j.jbiotec.2007.07.020

Neville FC, Broderick MJF, Vakourov AV, Millner PA Small solutions for greener chemistry None 108-114, 2007
View abstract

Bidoki SM, Lewis DM, Clark M, Vakorov A, Millner PA, McGorman D Ink-jet fabrication of electronic components J MICROMECH MICROENG 17 967-974, 2007
DOI:10.1088/0960-1317/17/5/017

Rodgers M, Millner P Biosensors: Rapid, preparation-free measurement EBR - European Biopharmaceutical Review 60-68, 2007
View abstract

Hannah MA, Kramer KM, Geffroy V, Kopka J, Blair MW, Erban A, Vallejos CE, Heyer AG, Sanders FET, Millner PA, Pilbeam DJ Hybrid weakness controlled by the dosage-dependent lethal (DL) gene system in common bean (Phaseolus vulgaris) is caused by a shoot-derived inhibitory signal leading to salicylic acid-associated root death NEW PHYTOL 176 537-549, 2007
DOI:10.1111/j.1469-8137.2007.02215.x

Garifallou GZ, Tsekenis G, Davis F, Higson SPJ, Millner PA, Pinacho DG, Sanchez-Baeza F, Marco MP, Gibson TD Labeless immunosensor assay for fluoroquinolone antibiotics based upon an AC impedance protocol ANAL LETT 40 1412-1422, 2007
DOI:10.1080/00032710701327070

Pchelintsev NA, Millner PA Development of surface activated screen-printed carbon transducers for biosensors application ANAL LETT 40 1317-1332, 2007
DOI:10.1080/00032710701326684

Hays HCW, Millner PA, Prodromidis MI Development of capacitance based immunosensors on mixed self-assembled monolayers SENSOR ACTUAT B-CHEM 114 1064-1070, 2006
DOI:10.1016/j.snb.2005.08.039

Hleli S, Martelet C, Abdelghani A, Bessueille F, Errachid A, Samitier J, Hays HCW, Millner PA, Bums N, Jaffrezic-Renault N An immunosensor for haemoglobin based on impedimetric properties of a new mixed self-assembled monolayer MATERIALS SCIENCE&ENGINEERING C-BIOMIMETIC AND SUPRAMOLECULAR SYSTEMS 26 322-327, 2006
DOI:10.1016/j.msec.2005.10.054

Hays HCW, Millner PA, Jones JK, Rayner-Brandes MH A novel and convenient self-drying system for bacterial preservation J MICROBIOL METH 63 29-35, 2005
DOI:10.1016/j.mimet.2005.02.014

Weiss S, Millner PA, Nelson AS Monitoring protein binding to phospholipid monolayers using electrochemical impedance spectroscopy Electrochimica Acta 50 4248-4256, 2005
DOI:10.1016/j.electacta.2005.05.045
View abstract

Vakurov A, Simpson CE, Daly CL, Gibson TD, Millner PA Acetylecholinesterase-based biosensor electrodes for organophosphate pesticide detection - II. Immobilization and stabilization of acetylecholinesterase BIOSENS BIOELECTRON 20 2324-2329, 2005
DOI:10.1016/j.bios.2004.07.022

Elnashar MM, Millner PA, Johnson AF, Gibson T Parallel plate equipment for preparation of uniform gel sheets Biotechnology Letters 27 737-739, 2005
DOI:10.1007/s10529-005-5363-0
View abstract

Vakurov A, Simpson CE, Daly CL, Gibson TD, Millner PA Acetylcholinesterase-based biosensor electrodes for organophosphate pesticide detection. I. Modification of carbon surface for immobilization of acetylcholinesterase. Biosens Bioelectron 20 1118-1125, 2004
DOI:10.1016/j.bios.2004.03.039
View abstract

Swarup R, Kargul J, Marchant A, Zadik D, Rahman A, Mills R, Yemm A, May S, Williams L, Millner PA, Tsurumi S, Moore I, Napier R, Kerr ID, Bennett MJ Structure-Function Analysis of the Presumptive Arabidopsis Auxin Permease AUX1 Plant Cell 16 3069-3083, 2004
DOI:10.1105/tpc.104.024737
View abstract

Robinson SA, Smith JE, Millner PA Toxoplasma gondii major surface antigen (SAG1): in vitro analysis of host cell binding. Parasitology 128 391-396, 2004
View abstract

Pritchard JA, Law KA, Vakurov A, Millner PA, Higson SPJ Sonochemically fabricated enzyme microelectrode arrays for the environmental moniroting of pesticides Biosensors and Bioelectronics 20 765-772, 2004
DOI:10.1016/j.bios.2004.06.008

Manios T, Stentiford EI, Millner PA Removal of heavy metals from a metaliferous water solution by Typha latifolia plants and sewage sludge compost Chemosphere 53 487-494, 2003
DOI:10.1016/S0045-6535(03)00537-X

Manios T, Stentiford EI, Millner PA The Removal of Chemical Oxygen Demand from Primary-Treated Domestic Wastewater in Subsurface-Flow Reed Beds Using Different Substrates Water Environment Research 75 336-341, 2003
DOI:10.2175/106143003X141141

Manios T, Stentiford EI, Millner PA The effect of heavy metals accumulation on the chlorophyll concentration of Typha latifolia plants, growing in a substrate containing sewage sludge compost and watered with metaliferus water Ecological Engineering 20 65-74, 2003
DOI:10.1016/S0925-8574(03)00004-1

Manios T, Stentiford EI, Millner PA Removal of total suspended solids from wastewater in constructed horizontal flow subsurface wetlands Journal of Environmental Science and Health. Part A. Toxic Hazardous Sustances and Environmental Engineering. 38 1073-1085, 2003

Manios T, Stentiford EI, Millner PA The removal of indicator microorganisms from primary treated wastewater in subsurface reed beds using different substrates ENVIRON TECHNOL 23 767-774, 2002

Mello LV, Millner PA, Findlay JBC Biochemical characteristics of guanine nucleotide binding protein alpha-subunit recombinant protein and three mutants: Investigation of a domain motion involved in GDP-GTP exchange J PROTEIN CHEM 21 29-34, 2002

Manios T, Stentiford EI, Millner PA The removal of NH3-N from primary treated wastewater in subsurface reed beds using different substrates J ENVIRON SCI HEAL A 37 297-308, 2002

Manios T, Stentiford EI, Millner PA The effect of heavy metals on the total protein concentration of Typha latifolia plants, growing in a substrate containing sewage sludge compost and watered with metaliferus wastewater Journal of Environmental Science and Health. Part A. Toxic Hazardous Sustances and Environmental Engineering. 37 1441-1451, 2002

Millner PA Heterotrimeric G-proteins in plant cell signaling NEW PHYTOL 151 165-174, 2001

Ricart CAO, Wise A, Findlay JBC, Millner PA Presence of rhodopsin-like proteins in Sorghum bicolor and Pisum sativum Journal of Plant Physiology 156 300-305, 2000

Manios T, Millner P, Stentiford EI Effect of rain and temperature on the performance of constructed reed beds Water Environment Research 72 305-312, 2000
DOI:10.2175/106143000X137527
View abstract

Millner PA, Carr TH Chapter 14 Molecular characteristics and cellular roles of guanine nucleotide binding proteins in plant cells New Comprehensive Biochemistry 33 315-336, 1999
DOI:10.1016/S0167-7306(08)60494-9

Hutton JL, Knight CD, Millner PA The Physcomitrella patens GPalpha1 homologue is located at protonemal cell junctions Journal of Experimental Botany 49 1113-1118, 1998

Wise A, Thomas PG, Carr TH, Murphy GA, Millner PA Expression of the Arabidopsis G-protein GPalpha1: purification and expression of the recombinant protein Plant Molecular Biology 33 723-728, 1997

Millner PA In vitro and in vivo analysis of plant g-protein linked pathways Biochemical Society Transactions 25, 1997
View abstract

Millner PA, Causier BE Q-protein coupled receptors in plant cells Journal of Experimental Botany 47 983-992, 1996
View abstract

Bennett MJ, Marchant A, Green HG, May ST, Ward SP, Millner PA, Walker AR, Schulz B, Feldmann KA Arabidopsis AUX1 gene: A permease-like regulator of root gravitropism Science 273 948-950, 1996
DOI:10.1126/science.273.5277.948
View abstract

Millner PA, Groarke DA, White IR Synthetic peptides as probes of plant cell signalling: the auxin signalling pathway Plant Growth Regulation 18 143-147, 1996
View abstract

Millner PA, Causier BE G-protein coupled receptors in plant cells Journal of Experimental Botany 47 983-992, 1996

Barbier-Brygoo H, Zimmerman S, Thomine S, White I, Millner PA, Guern J Elementary auxin response chains at the plasma membrane involve external abp 1 and multiple electrogenic ion transport proteins Plant Growth Regulation 18 23-28, 1996

Bennett MJ, Marchant A, Green HG, May ST, Ward SP, Millner PA, Walker AR, Schulz B, Feldmann KA Arabidopsis AUX1 gene: A permease-like regulator of root gravitropism Science 273 948-950, 1996

Causier BE, Millner PA G-protein regulated phopsholipase D - another piece in the plant cell signalling jigsaw Trends in Plant Science 1 168-170, 1996

Millner PA The auxin signal Current Opinion in Cell Biology 7 224-231, 1995
DOI:10.1016/0955-0674(95)80032-8
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Drøbak BK, Watkins PAC, Bunney TD, Dove SK, Shaw PJ, White IR, Millner PA Association of multiple GTP-binding proteins with the plant cytoskeleton and nuclear matrix Biochemical and Biophysical Research Communications 210 7-13, 1995
DOI:10.1006/bbrc.1995.1620
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Ricart CAO, White IR, Findlay JBC, Keen JN, Millner PA Detection and Purification of a Putative Gα<inf>O</inf>-protein from Sorghum bicolor Journal of Plant Physiology 146 645-651, 1995
DOI:10.1016/S0176-1617(11)81927-9
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Wise A, Thomas PG, White IR, Millner PA Isolation of a putative receptor from Zea mays microsomal membranes that interacts with the G-protein, GPα1 FEBS Letters 356 233-237, 1994
DOI:10.1016/S0014-5793(94)80076-6
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Millner PA, White IR, Groarke DA, Theil G, Blatt MR Novel strategies to an auxin-evoked transport control. Symposia of the Society for Experimental Biology 48 203-213, 1994
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Fricker MD, White NS, Thiel G, Millner P, Blatt MR Peptides derived from the auxin binding protein elevate Ca2+ and pH in stomatal guard cells of Vicia faba: a confocal fluorescence ratio imaging study. Symposia of the Society for Experimental Biology 48 215-228, 1994
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Finan PM, White IR, Redpath SH, Findlay JBC, Millner PA Molecular cloning, sequence determination and heterologous expression of nucleoside diphosphate kinase from Pisum sativum Plant Molecular Biology 25 59-67, 1994
DOI:10.1007/BF00024198
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Thiel G, Blatt MR, Fricker MD, White IR, Millner P Modulation of K<sup>+</sup>channels in Vicia stomatal guard cells by peptide homologs to the auxin-binding protein C terminus Proceedings of the National Academy of Sciences of the United States of America 90 11493-11497, 1993
DOI:10.1073/pnas.90.24.11493
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Ishak Z, White LR, Millner PA Molecular interaction of LHC II kinase with petD peptides, 1993

White IR, Wise A, Millner PA Evidence for G-protein-linked receptors in higher plants: stimulation of GTP-gamma-S binding to membrane fractions by the mastoparan analogue mas 7 Planta 191 285-288, 1993
DOI:10.1007/BF00199762
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Wise A, White IR, Millner PA Stimulation of guanosine 5'-O-(3-thio)triphosphate binding to higher plant plasma membranes by the mastoparan-analogue, Mas 7, 1993

Ricart CA, Morhy L, White IR, Findlay JBC, Millner PA Specific immunodetection and purification of G proteins from Sorghum bicolor, 1993

White IR, Finan PM, Millner PA Nucleoside Diphosphate Kinase Associated with Pisum sativum Microsomal Membranes: Apparent Binding of GTPγS at nM Concentrations Journal of Plant Physiology 142 191-196, 1993
DOI:10.1016/S0176-1617(11)80962-4
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Groarke A, Webster J, White IR, Millner PA Identification of a membrane receptor for the auxin binding protein, 1993

Ishak Z, White IR, Millner PA Sensitivity of protein phosphorylation in tobacco thylakoids to protein kinase C inhibitors and synthetic peptides, 1992

Finan PM, White IR, Findlay JBC, Millner PA Identification of nucleoside diphosphate kinase from Pea microsomal membrane, 1992

Wise A, Millner PA Evidence for the presence of GTP-binding proteins in tobacco leaf and maize hypocotyl plasmalemma, 1992

Clarkson J, White IR, Millner PA Specific immune detection and partial purification of G-proteins from Arabidopsis thailana, 1992

Clarkson J, Finan PM, Ricart CA, White IR, Millner PA Specific immunodetection of G-proteins in plant cell membranes, 1991

White IR, O'Donnell PJ, Keen JN, Findlay JBC, Millner PA Investigation of the substrate specificity of thylakoid protein kinase using synthetic peptides FEBS Letters 269 49-52, 1990
DOI:10.1016/0014-5793(90)81116-6
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Millner PA, Robinson PS ADP-ribosylation of thylakoid membrane polypeptides by cholera toxin is correlated with inhibition of thylakoid GTPase activity and protein phosphorylation Cellular Signalling 1 421-433, 1989
DOI:10.1016/0898-6568(89)90028-4
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Millner PA Are guanine nucleotide-binding proteins involved in regulation of thylakoid protein kinase activity? FEBS Letters 226 155-160, 1987
DOI:10.1016/0014-5793(87)80570-7
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Millner PA, Gogel G, Barber J Investigation of the spatial relationships between photosystem 2 polypeptides by reversible crosslinking and diagonal electrophoresis Photosynthesis Research 13 185-198, 1987
DOI:10.1007/BF00035240
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Millner PA, Marder JB, Gounaris K, Barber J Localization and identification of phosphoproteins within the Photosystem II core of higher-plant thylakoid membranes BBA - Bioenergetics 852 30-37, 1986
DOI:10.1016/0005-2728(86)90053-8
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Telfer A, Hodges M, Millner PA, Barber J The cation-dependence of the degree of protein phosphorylation-induced unstacking of pea thylakoids BBA - Bioenergetics 766 554-562, 1984
DOI:10.1016/0005-2728(84)90114-2
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Millner PA, Chapman DJ, Barber J The effect of chloroplast coupling factor ATP synthetase (CF<inf>1</inf>· CF<inf>0</inf>) reconstitution on fluidity properties of isolated thylakoid lipid vesicles BBA - Bioenergetics 765 282-287, 1984
DOI:10.1016/0005-2728(84)90167-1
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Barber J, Ford RC, Mitchell RAC, Millner PA Chloroplast thylakoid membrane fluidity and its sensitivity to temperature Planta 161 375-380, 1984
DOI:10.1007/BF00398729
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Millner PA, Barber J Plastoquinone as a mobile redox carrier in the photosynthetic membrane FEBS Letters 169 1-6, 1984
DOI:10.1016/0014-5793(84)80277-X
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Millner PA, Mitchell RAC, Chapman DJ, Barber J Fluidity properties of isolated chloroplast thylakoid lipids Photosynthesis Research 5 63-76, 1984
DOI:10.1007/BF00018375
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Millner PA, Grouzis JP, Chapman DJ, Barber J Lipid enrichment of thylakoid membranes. I. Using soybean phospholipids BBA - Bioenergetics 722 331-340, 1983
DOI:10.1016/0005-2728(83)90081-6
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Millner PA, Widger WR, Abbott MS, Cramer WA, Dilley RA The effect of adenine nucleotides on inhibition of the thylakoid protein kinase by sulfhydryl-directed reagents. Journal of Biological Chemistry 257 1736-1742, 1982

Dilley RA, Prochaska LJ, Baker GM, Tandy NE, Millner PA Proton-Membrane Interactions in Chloroplast Bioenergetics Current Topics in Membranes and Transport 16 345-369, 1982
DOI:10.1016/S0070-2161(08)60709-1
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Laszlo JA, Millner PA, Dilley RA Light-dependent chemical modification of thylakoid membrane proteins with carboxyl-directed reagents Archives of Biochemistry and Biophysics 215 571-581, 1982
DOI:10.1016/0003-9861(82)90118-7
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Goode JA Development of biosensors using novel bioreceptors; Investigation and optimisation of fundamental parameters at the nanoscale
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