Faculty of Biological Sciences

Dr Iain Manfield

BSc, Brunel; PhD, Leeds.
Technology facility manager
School of Molecular and Cellular Biology

Background: PhD with Professor Peter Stockley, 1992. Post doc work over-expressing and purifying DNA-binding proteins; characterising properties of DNA-protein complexes in vitro.

Contact:  Astbury 9.108, +44(0) 113 34 37279, email address for  

Research Interests

Currently running our Centre for Biomolecular Interactions technology facility, which provides access and training to use equipment for purifying molecules and analysing the interactions between molecules. Biophysical analysis of molecules and interactions

Purification using HPLC and FPLC.

Analysis of molecular interactions and protein properties using SPR, ITC, DSC and AUC.

 

Studentship information

See also:

Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, Alqallaf D, Roberts APE, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC Affimer proteins are versatile and renewable affinity reagents eLife 6 -, 2017
DOI:10.7554/eLife.24903
View abstract

Omanovic-Miklicanin E, Manfield I, Wilkins T Application of isothermal titration calorimetry in evaluation of protein–nanoparticle interactions Journal of Thermal Analysis and Calorimetry 127 605-613, 2017
DOI:10.1007/s10973-016-5764-4
View abstract

Maqbool A, Spary EJ, Manfield IW, Ruhmann M, Zuliani-Alvarez L, Gamboa-Esteves FO, Porter KE, Drinkhill MJ, Midwood KS, Turner NA Tenascin C upregulates interleukin-6 expression in human cardiac myofibroblasts via toll-like receptor 4. World Journal of Cardiology 8 340-350, 2016
DOI:10.4330/wjc.v8.i5.340
View abstract

RolfssonÓ, Middleton S, Manfield IW, White SJ, Fan B, Vaughan R, Ranson NA, Dykeman E, Twarock R, Ford J, Cheng Kao C, Stockley PG Direct Evidence for Packaging Signal-Mediated Assembly of Bacteriophage MS2 Journal of Molecular Biology 428 431-448, 2016
DOI:10.1016/j.jmb.2015.11.014
View abstract

Stockley PG, White SJ, Dykeman E, Manfield I, Rolfsson O, Patel N, Bingham R, Barker A, Wroblewski E, Chandler-Bostock R, Weiß EU, Ranson NA, Tuma R, Twarock R Bacteriophage MS2 genomic RNA encodes an assembly instruction manual for its capsid. Bacteriophage 6 e1157666-, 2016
View abstract

Edwards JH, Herbert A, Jones GL, Manfield IW, Fisher J, Ingham E The effects of irradiation on the biological and biomechanical properties of an acellular porcine superflexor tendon graft for cruciate ligament repair Journal of Biomedical Materials Research - Part B Applied Biomaterials -, 2016
DOI:10.1002/jbm.b.33786
View abstract

Adams S, Manfield I, Stockley P, Carré IA Revised Morning Loops of the Arabidopsis Circadian Clock Based on Analyses of Direct Regulatory Interactions PLoS ONE 10 -, 2015
DOI:10.1371/journal.pone.0143943
View abstract

Fisher MJ, Williamson DJ, Burslem GM, Plante JP, Manfield IW, Tiede C, Ault JR, Stockley PG, Plein S, Maqbool A, Tomlinson DC, Foster R, Warriner SL, Bon RS Trivalent Gd-DOTA reagents for modification of proteins RSC Advances 5 96194-96200, 2015
DOI:10.1039/c5ra20359g
View abstract

Edwards J, Herbert A, Manfield I, Fisher J, Ingham E Irradiation of acellular porcine superflexor tendons. [DATASET], 2015
DOI:10.5518/30

Cornuault V, Manfield IW, Ralet M-C, Knox JP Epitope detection chromatography: a method to dissect the structural heterogeneity and inter-connections of plant cell-wall matrix glycans. Plant J 78 715-722, 2014
DOI:10.1111/tpj.12504
View abstract

Boer DR, Freire-Rios A, van den Berg WAM, Saaki T, Manfield IW, Kepinski S, López-Vidrieo I, Franco-Zorrilla JM, de Vries SC, Solano R, Weijers D, Coll M Structural basis for DNA binding specificity by the auxin-dependent ARF transcription factors. Cell 156 577-589, 2014
DOI:10.1016/j.cell.2013.12.027
View abstract

Roberts GA, Chen K, Bower EKM, Madrzak J, Woods A, Barker AM, Cooper LP, White JH, Blakely GW, Manfield I, Dryden DTF Mutations of the domain forming the dimeric interface of the ArdA protein affect dimerization and antimodification activity but not antirestriction activity. FEBS J 280 4903-4914, 2013
DOI:10.1111/febs.12467
View abstract

Lee KJD, Cornuault V, Manfield IW, Ralet M-C, Knox JP Multi-scale spatial heterogeneity of pectic rhamnogalacturonan I (RG-I) structural features in tobacco seed endosperm cell walls. Plant J 75 1018-1027, 2013
DOI:10.1111/tpj.12263
View abstract

King R, Tiede C, McPherson M, Manfield I, Fishwick C, Tomlinson D, Ajjan R A novel methodology for modulation of fibrin clot lysis: the role of fibrinogen-targeted artificial binding proteins, 2013

Jithesh MN, Wally OSD, Manfield I, Critchley AT, Hiltz D, Prithiviraj B Analysis of seaweed extract-induced transcriptome leads to identification of a negative regulator of salt tolerance in Arabidopsis HortScience 47 704-709, 2012
View abstract

Gruszka DT, Wojdyla JA, Bingham RJ, Turkenburg JP, Manfield IW, Steward A, Leech AP, Geoghegan JA, Foster TJ, Clarke J, Potts JR Staphylococcal biofilm-forming protein has a contiguous rod-like structure. Proc Natl Acad Sci U S A 109 E1011-E1018, 2012
DOI:10.1073/pnas.1119456109
View abstract

Rice GI, Bond J, Asipu A, Brunette RL, Manfield IW, Carr IM, Fuller JC, Jackson RM, Lamb T, Briggs TA, Ali M, Gornall H, Couthard LR, Aeby A, Attard-Montalto SP, Bertini E, Bodemer C, Brockmann K, Brueton LA, Corry PC, Desguerre I, Fazzi E, Cazorla AG, Gener B, Hamel BCJ, Heiberg A, Hunter M, van der Knaap MS, Kumar R, Lagae L, Landrieu PG, Lourenco CM, Marom D, McDermott MF, van der Merwe W, Orcesi S, Prendiville JS, Rasmussen M, Shalev SA, Soler DM, Shinawi M, Spiegel R, Tan TY, Vanderver A, Wakeling EL, Wassmer E, Whittaker E, Lebon P, Stetson DB, Bonthron DT, Crow YJ Mutations involved in Aicardi-Goutières syndrome implicate SAMHD1 as regulator of the innate immune response. Nat Genet 41 829-832, 2009
DOI:10.1038/ng.373
View abstract

Needham CJ, Manfield IW, Bulpitt AJ, Gilmartin PM, Westhead DR From gene expression to gene regulatory networks in Arabidopsis thaliana BMC Systems Biology 3:85 -, 2009
DOI:10.1186/1752-0509-3-85

Manfield IW, Stockley PG Ethylation interference footprinting of DNA-protein complexes. Methods Mol Biol 543 105-120, 2009
DOI:10.1007/978-1-60327-015-1_9
View abstract

Li J, Webster M, Dudas B, Cook H, Manfield I, Davies B, Gilmartin PM The S locus-linked Primula homeotic mutant sepaloid shows characteristics of a B-function mutant but does not result from mutation in a B-function gene PLANT J 56 1-12, 2008
DOI:10.1111/j.1365-313X.2008.03584.x

Westhead DR, Manfield IW, Needham CJ Bioinformatic approaches to biological systems., 2008

Manfield IW, Devlin PF, Jen CH, Westhead DR, Gilmartin PM Conservation, convergence, and divergence of light-responsive, circadian-regulated, and tissue-specific expression patterns during evolution of the Arabidopsis GATA gene family Plant Physiology 143 941-958, 2007
DOI:10.1104/pp.106.090761
View abstract

Hadden DA, Phillipson BA, Johnston KA, Brown LA, Manfield IW, El-Shami MI, Sparkes IA, Baker A Arabidopsis PEX19 is a dimeric protein that binds the peroxin PEX10 Molecular Membrane Biology 23 325-336, 2006
DOI:10.1080/09687860600738221
View abstract

Manfield IW, Jen CH, Pinney JW, Michalopoulos I, Bradford JR, Gilmartin PM, Westhead DR Arabidopsis Co-expression Tool (ACT): web server tools for microarray-based gene expression analysis Nucleic Acids Research 34 504-509, 2006

Marincs F, Manfield IW, Stead JA, McDowall KJ, Stockley PG Transcript analysis reveals an extended regulon and the importance of protein-protein co-operativity for the Escherichia coli methionine repressor. Biochem J 396 227-234, 2006
DOI:10.1042/BJ20060021
View abstract

Jen CH, Manfield IW, Michalopoulos I, Pinney JW, Willats WGT, Gilmartin PM, Westhead DR The Arabidopsis co-expression tool ((ACT)): a WWW-based tool and database for microarray-based gene expression analysis PLANT J 46 336-348, 2006
DOI:10.1111/j.1365-313X.2006.02681.x

Manfield IW, Bernal AJ, Moller I, McCartney L, Riess NP, Knox JP, Willats WGT Re-engineering of the PAM1 phage display monoclonal antibody to produce a soluble, versatile anti-homogalacturonan scFv Plant Science 169 1090-1095, 2005
DOI:10.1016/j.plantsci.2005.07.008
View abstract

Manfield IW, Pavlov VK, Li JH, Cook HE, Hummel F, Gilmartin PM Molecular characterization of DNA sequences from the Primula vulgaris S-locus J EXP BOT 56 1177-1188, 2005
DOI:10.1093/jxb/eri110

Manfield IW, Orfila C, McCartney L, Harholt J, Bernal AJ, Scheller HV, Gilmartin PM, Mikkelsen JD, Knox JP, Willats WGT Novel cell wall architecture of isoxaben-habituated Arabidopsis suspension-cultured cells: global transcript profiling and cellular analysis PLANT J 40 260-275, 2004
DOI:10.1111/j.1365-313X.2004.02208.x

Chant A, Provatopoulou X, Manfield IW, Kneale GG Structural and functional characterisation of the DNA binding domain of the Aspergillus nidulans gene regulatory protein AreA BBA-PROTEINS PROTEOM 1648 84-89, 2003
DOI:10.1016/S1570-9639(03)00109-2

Yoburn JC, Deb S, Manfield IW, Stockley PG, Van Vranken DL A biaryl peptide crosslink in a MetJ peptide model confers cooperative, nonspecific binding to DNA that ablates both repressor binding and in vitro transcription. Bioorg Med Chem 11 811-816, 2003
View abstract

Teakle GR, Manfield IW, Graham JF, Gilmartin PM Arabidopsis thaliana GATA factors: organisation, expression and DNA-binding characteristics Plant Molecular Biology 50 43-56, 2002
DOI:10.1023/A:1016062325584

He Y-Y, Garvie CW, Elworthy S, Manfield IW, McNally T, Lawrenson ID, Phillips SEV, Stockley PG Structural and functional studies of an intermediate on the pathway to operator binding by Escherichia coli MetJ. J Mol Biol 320 39-53, 2002
DOI:10.1016/S0022-2836(02)00423-0
View abstract

Manfield IW, Stockley PG Ethylation interference. Methods Mol Biol 148 229-243, 2001
DOI:10.1385/1-59259-208-2:229