Faculty of Biological Sciences

Dr Jonathan Lippiat

1993-2000 BSc & PhD Physiology (University of Leicester)
Lecturer
School of Biomedical Sciences

Background: 1993-2000 BSc & PhD Physiology, University of Leicester. 2001-2004 Postdoctoral Scientist, University of Oxford. 2002-2004 Junior Research Fellow, Linacre College Oxford.

Contact:  Garstang G6.44b, +44(0) 113 34 34236, email address for  

Research Interests

Molecular properties and pharmacology of ion channels and transporters

I have wide-ranging interests in ion channel properties, structure, and pharmacology. We mainly focus on potassium channels, but also conduct research on calcium channels, chloride channels and transporters. Much of our research is based on ion channels that have been implicated in human disorders or can be targetted by potential therapeutics.

 

 Ion Channel Research @ Leeds

 Editor, Potassium Channels: Methods and Protocols

 

 

Current Projects

Current projects include (& more details to come soon)

 

1. Identification and characterisation of ion channel-binding Affimer proteins

 

2. KNa1.1 channels, KCNT1, and epilepsy

 

3. Structural studies of human ion channels

 

4. Potassium channel pharmacology

 

5. CLC-5 function, Dent's diease, and emdocytosis

 

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Undergraduate research projects involve investigating the pharmacology and physiological roles of human ion channels and transporters.

Postgraduate studentship areas:

  • Applications are welcome for postgraduate research projects on ion channels and transporters in any of these areas, leading to the degree of PhD or MSc by Research. Please contact by email for more information.

See also:

Modules managed

BMSC2226 - Experimental Skills in Pharmacology
BMSC3145 - Advanced Topics in Physiology I
BMSC3235 - Advanced Topics in Physiology II

Modules taught

BMSC1212 - Introduction to Pharmacology
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2120 - Scientific Skills
BMSC2223 - Neuropharmacology
BMSC2226 - Experimental Skills in Pharmacology
BMSC2230 - Topics in Pharmacology
BMSC3140 - Advanced Scientific Skills
BMSC3143/44/45/46 E - ATU - Fundamentals of ion channel biology
BMSC3233/34/35/36 - Advanced topics for BMS students II
BMSC3301 - Research Project in Biomedical Sciences
BMSC3302 - Medical Pharmacology
BMSC3399 - Extended Research Project Preparation
BMSC5382M - Extended Research Project

Academic roles

UG Programme Leader - UG Human Physiology programmes

Committees

Member of Undergraduate School Taught Student Education Committee (Chair of Special Cases Committees: Medical Sciences, Human Physiology, Pharmacology & Neuroscience)

Centre membership: Neuroscience Research at Leeds (NeuR@L)

Group Leader Dr Jonathan Lippiat  (Lecturer)

Molecular properties and pharmacology of ion channels and transporters 

Postgraduates

Bethan Cole (Primary supervisor) 65% FTE
Laura Musgrove (Primary supervisor) 50% FTE
Robert Bedford (Co-supervisor) 50% FTE
Simon Davies (Co-supervisor) 50% FTE
Hayley Pearson (Co-supervisor) 33% FTE
Shihab Shah (Co-supervisor) 25% FTE

Al-Owais MM, Hettiarachchi NT, Boyle JP, Scragg JL, Elies J, Dallas ML, Lippiat JD, Steele DS, Peers C Multiple mechanisms mediating carbon monoxide inhibition of the voltage-gated K + channel Kv1.5 Cell Death and Disease 8 -, 2017
DOI:10.1038/cddis.2017.568
View abstract

Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, Alqallaf D, Roberts APE, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC Affimer proteins are versatile and renewable affinity reagents eLife 6 -, 2017
DOI:10.7554/eLife.24903
View abstract

Rawson S, Davies S, Lippiat JD, Muench SP The changing landscape of membrane protein structural biology through developments in electron microscopy Molecular Membrane Biology 33 12-22, 2016
DOI:10.1080/09687688.2016.1221533
View abstract

Wrighton DC, Muench SP, Lippiat JD Mechanism of inhibition of mouse Slo3 (K<inf>Ca</inf>5.1) potassium channels by quinine, quinidine and barium British Journal of Pharmacology 172 4355-4363, 2015
DOI:10.1111/bph.13214
View abstract

Igloi Z, Mohl BP, Lippiat JD, Harris M, Mankouri J Requirement for chloride channel function during the hepatitis C virus life cycle Journal of Virology 89 4023-4029, 2015
DOI:10.1128/JVI.02946-14
View abstract

Alexander SPH, Benson HE, Faccenda E, Pawson AJ, Sharman JL, McGrath JC, Catterall WA, Spedding M, Peters JA, Harmar AJ, CGTP Collaborators, Abul-Hasn N, Anderson CM, Anderson CMH, Araiksinen MS, Arita M, Arthofer E, Barker EL, Barratt C, Barnes NM, Bathgate R, Beart PM, Belelli D, Bennett AJ, Birdsall NJM, Boison D, Bonner TI, Brailsford L, Bröer S, Brown P, Calo G, Carter WG, Catterall WA, Chan SLF, Chao MV, Chiang N, Christopoulos A, Chun JJ, Cidlowski J, Clapham DE, Cockcroft S, Connor MA, Cox HM, Cuthbert A, Dautzenberg FM, Davenport AP, Dawson PA, Dent G, Dijksterhuis JP, Dollery CT, Dolphin AC, Donowitz M, Dubocovich ML, Eiden L,Eidne K, Evans BA, Fabbro D, Fahlke C, Farndale R, Fitzgerald GA, Fong TM, Fowler CJ, Fry JR, Funk CD, Futerman AH, Ganapathy V, Gaisnier B, Gershengorn MA, Goldin A, Goldman ID, Gundlach AL, Hagenbuch B, Hales TG, Hammond JR, Hamon M, Hancox JC, Hauger RL, Hay DL, Hobbs AJ, Hollenberg MD, HollidayND, Hoyer D, Hynes NA, Inui K-I, Ishii S, Jacobson KA, Jarvis GE, Jarvis MF, Jensen R, Jones CE, Jones RL, Kaibuchi K, Kanai Y, Kennedy C, Kerr ID, Khan AA, Klienz MJ, Kukkonen JP, Lapoint JY, Leurs R, and 82 other authors The Concise Guide to PHARMACOLOGY 2013/14: overview. British journal of pharmacology 170 1449-1458, 2013
DOI:10.1111/bph.12444
View abstract

Lippiat JD Chloride ion channels and transporters: From curiosities of nature and source of human disease to drug targets European Pharmaceutical Review 18 18-22, 2013
View abstract

Jin X, Shah S, Liu Y, Zhang H, Lees M, Fu Z, Lippiat JD, Beech DJ, Sivaprasadarao A, Baldwin SA, Zhang H, Gamper N Activation of the Cl- channel ANO1 by localized calcium signals in nociceptive sensory neurons requires coupling with the IP3 receptor. Sci Signal 6 ra73-, 2013
DOI:10.1126/scisignal.2004184
View abstract

Maher J, Hunter AC, Mabley JG, Lippiat J, Allen MC Smooth muscle relaxation and activation of the large conductance Ca(++)-activated K+ (BK(Ca)) channel by novel oestrogens. Br J Pharmacol 169 1153-1165, 2013
DOI:10.1111/bph.12211
View abstract

Gorvin CM, Wilmer MJ, Piret SE, Harding B, van den Heuvel LP, Wrong O, Jat PS, Lippiat JD, Levtchenko EN, Thakker RV Receptor-mediated endocytosis and endosomal acidification is impaired in proximal tubule epithelial cells of Dent disease patients. Proc Natl Acad Sci U S A 110 7014-7019, 2013
DOI:10.1073/pnas.1302063110
View abstract

Lippiat JD, Wrighton DC Conventional micropipette-based patch clamp techniques. Methods Mol Biol 998 91-107, 2013
DOI:10.1007/978-1-62703-351-0_7
View abstract

Wong CC, Akiyama Y, Abe T, Lippiat JD, Orfila C, Williamson G Carrier-mediated transport of quercetin conjugates: involvement of organic anion transporters and organic anion transporting polypeptides. Biochem Pharmacol 84 564-570, 2012
DOI:10.1016/j.bcp.2012.05.011
View abstract

Sukumar P, Sedo A, Li J, Wilson LA, O'Regan D, Lippiat JD, Porter KE, Kearney MT, Ainscough JFX, Beech DJ Constitutively active TRPC channels of adipocytes confer a mechanism for sensing dietary fatty acids and regulating adiponectin. Circ Res 111 191-200, 2012
DOI:10.1161/CIRCRESAHA.112.270751
View abstract

Lippiat JD, Smith AJ The CLC-5 2Cl-/H+ exchange transporter in endosomal function and Dent's disease Frontiers in Physiology 3 -, 2012
DOI:10.3389/fphys.2012.00449
View abstract

Smith AJ, Lippiat JD Voltage-dependent charge movement associated with activation of the CLC-5 2Cl(-)/1H(+) exchanger FASEB J 24 3696-3705, 2010
DOI:10.1096/fj.09-150649

Smith AJ, Lippiat JD Direct endosomal acidification by the outwardly rectifying CLC-5 Cl-/H+ exchanger J PHYSIOL-LONDON 588 2033-2045, 2010
DOI:10.1113/jphysiol.2010.188540

Manna PT, Smith AJ, Taneja TK, Howell GJ, Lippiat JD, Sivaprasadarao A Constitutive Endocytic Recycling and Protein Kinase C-mediated Lysosomal Degradation Control K-ATP Channel Surface Density J BIOL CHEM 285 5963-5973, 2010
DOI:10.1074/jbc.M109.066902

Reed AAC, Loh NY, Terryn S, Lippiat JD, Partridge C, Galvanovskis J, Williams SE, Jouret F, Wu FTF, Courtoy PJ, Nesbit MA, Rorsman P, Devuyst O, Ashcroft FM, Thakker RV CLC-5 and KIF3B interact to facilitate CLC-5 plasma membrane expression, endocytosis, and microtubular transport: relevance to pathophysiology of Dent's disease AM J PHYSIOL-RENAL 298 F365-F380, 2010
DOI:10.1152/ajprenal.00038.2009

Smith AJ, Reed AAC, Loh NY, Thakker RV, Lippiat JD Characterization of Dent's disease mutations of CLC-5 reveals a correlation between functional and cell biological consequences and protein structure AM J PHYSIOL-RENAL 296 F390-F397, 2009
DOI:10.1152/ajprenal.90526.2008

Wu F, Reed AAC, Williams SE, Loh NY, Lippiat JD, Christie PT, Large O, Bettinelli A, Dillon MJ, Goldraich NP, Hoppe B, Lhotta K, Loirat C, Malik R, Morel D, Kotanko P, Roussel B, Rubinger D, Schrander-Stumpel C, Serdaroglu E, Nesbit MA, Ashcroft F, Thakker RV Mutational Analysis of CLC-5, Cofilin and CLC-4 in Patients with Dent's Disease NEPHRON PHYSIOL 112 P53-P62, 2009
DOI:10.1159/000225944

Lippiat JD Potassium Channels: Methods and Protocols - Preface Methods in Molecular Biology 491 -, 2008

de Wet H, Lippiat JD, Allen M Analysing steroid modulation of BK(Ca) channels reconstituted into planar lipid bilayers. Methods Mol Biol 491 177-186, 2008
DOI:10.1007/978-1-59745-526-8_14
View abstract

Lippiat JD Whole-cell recording using the perforated patch clamp technique. Methods Mol Biol 491 141-149, 2008
DOI:10.1007/978-1-59745-526-8_11
View abstract

De Wet H, Allen M, Holmes C, Stobbart M, Lippiat JD, Callaghan R Modulation of the BK channel by estrogens: examination at single channel level MOL MEMBR BIOL 23 420-429, 2006
DOI:10.1080/09687860600802803

Shimomura K, Girard CAJ, Proks P, Nazim J, Lippiat JD, Cerutti F, Lorini R, Ellard S, Hattersely AT, Barbetti F, Ashcroft FM Mutations at the same residue (R50) of Kir6.2 (KCNJ11) that cause neonatal diabetes produce different functional effects DIABETES 55 1705-1712, 2006
DOI:10.2337/db05-1640

Proks P, Lippiat JD Membrane ion channels and diabetes CURR PHARM DESIGN 12 485-501, 2006

Toye AA, Lippiat JD, Proks P, Shimomura K, Bentley L, Hugill A, Mijat V, Goldsworthy M, Moir L, Haynes A, Quarterman J, Freeman HC, Ashcroft FM, Cox RD A genetic and physiological study of impaired glucose homeostasis control in C57BL/6J mice DIABETOLOGIA 48 675-686, 2005
DOI:10.1007/s00125-005-1680-z

Proks P, Antcliff JF, Lippiat J, Gloyn AL, Hattersley AT, Ashcroft FM Molecular basis of Kir6.2 mutations associated with neonatal diabetes or neonatal diabetes plus neurological features P NATL ACAD SCI USA 101 17539-17544, 2004
DOI:10.1073/pnas.0404756101

Campbell JD, Proks P, Lippiat JD, Sansom MSP, Ashcroft FM Identification of a functionally important negatively charged residue within the second catalytic site of the SUR1 nucleotide-bindind domains DIABETES 53 S123-S127, 2004
View abstract

Tsuboi T, Lippiat JD, Ashcroft FM, Rutter GA ATP-dependent interaction of the cytosolic domains of the inwardly rectifying K+ channel Kir6.2 revealed by fluorescence resonance energy transfer P NATL ACAD SCI USA 101 76-81, 2004
DOI:10.1073/pnas.0306347101

Lippiat JD, Standen NB, Harrow ID, Phillips SC, Davies NW Properties of BKCa channels formed by bicistronic expression of hSlo alpha and beta 1-4 subunits in HEK293 cells J MEMBRANE BIOL 192 141-148, 2003
DOI:10.1007/s00232-002-1070-0

Lippiat JD, Albinson SL, Ashcroft FM Interaction of the cytosolic domains of the Kir6.2 subunit of the K-ATP channel is modulated by sulfonylureas DIABETES 51 S377-S380, 2002
View abstract

Lippiat JD, Standen NB, Davies NW A residue in the intracellular vestibule of the pore is critical for gating and permeation in Ca2+-activated K+ (BKCa) channels Journal of Physiology 829 131-138, 2000

Lippiat JD, Standen NB, Davies NW Block of cloned BKca2 channels (rSlo) expressed in HEK 293 cells by N-methyl D-glucamine Pflugers Archiv: European Journal of Physiology 436 810-812, 1998

Gauer J, Tumova S, Gardner S, Kerimi A, Lippiat J, McKeown L, Williamson G Effect of Polyphenols on Sugar Transport by GLUT2, GLUT5 and GLUT7,