Faculty of Biological Sciences

Dr Lin-Hua Jiang

BSc, MSc, East China Normal University, China; PhD, Leeds
Lecturer
School of Biomedical Sciences

Background: Postdoctoral Research Fellow (1999-2003), Department of Biomedical Science, University of Sheffield; University Research Fellow (2004-2008), School of Biomedical Sciences, University of Leeds; Lecturer (2006-), School of Biomedical Sciences, University of Leeds

Contact:  Garstang 6.44e, +44(0) 113 34 34231, email address for  

You can read more about Dr Jiang's interests here:
http://community.frontiersin.org/people/Lin-HuaJiang/61808
http://scholar.google.co.uk/citations?user=EtFDgU4AAAAJ&hl=en
http://www.fbs.leeds.ac.uk/research/bulletin/index.php?id=1401

Research Interests

Molecular and structural mechanisms of calcium-permeable ion channels in health and disease

Research in my lab aims to elucidate the molecular and structural mechanisms of calcium-permeable channels in health and disease and develop novel therapeutics, with the following research interests.

TRPM2 channel and ROS signalling in neurodegeneration

Transient receptor potential melastatin 2 (TRPM2) channel is gated by cytosolic ADP-ribose. Our studies have contributed in understanding the molecular basis determining TRPM2 channel assembly, ion permeation, ligand binding, and functional regulation (Mei et al., 2006; Xia et al., 2008; Yang et al., 2010, 2011; Zou et al., 2011; Yu et al., 2014, 2017). Currently, we are interested in studying the molecular and structural basis for ligand binding, channel gating and allosteric modulation using molecular biology, structural biology and electrophysiology.

TRPM2 channels are strongly activated in response to elevated levels of ROS and act as a molecular sensor for oxidative stress under diverse pathophysiological settings (Jiang et al., 2010; Li et al., 2015; Syed Mortadza et al., 2015). TRPM2 channels have been shown to be important in mediating ROS-induced cell death (Zhou et al., 2013; Ye et al., 2014; Manna et al., 2015; Yu et al., 2015;Syed Mortadza et al., 2017).  We are currently investigating the TRPM2 mechanisms underpinning brain diseases such as ischemic stroke, Alzheimer's, Parkinson's, Huntington's, Multiple Sclerosis and Amyotropic Lateral Sclerosis.

There is no TRPM2 specific inhibitor or antagonist (Jiang et al., 2010). We are working along with colleagues in medicine chemistry in developing TRPM2 channel inhibitors as research tools and therapeutics, and in combining molecular biology, structural biology and electrophysiology to understand the mechanisms of their actions.

P2X7 receptor and ATP signalling in immunity, inflammatory diseases and cancer

P2X7 receptor, while belonging to the ATP-gated ion channel P2X receptor family, exhibits unique functional and pharmacological properties (Jiang et al., 2013; Wei et al., 2016). We are interested in the structural basis that governs ligand binding, ion channel gating and large pore formation (Caseley et al., 2015; 2017), using site-directed mutagenesis and electrophysiology in combination with cryo-EM and structural modelling.

P2X7 receptor plays a critical role in immune response. Change in the P2X7 receptor expression and/or function has been causatively associated with diverse inflammatory diseases (Jiang et al., 2013; Caseley et al., 2014; Roger et al., 2015).  The gene encoding the human P2X7 receptor is prolific with non-synonymous single nucleotide polymorphisms (NS-SNP); expression of such gain/loss-of-functional NS-SNPs alters the susceptibility of individuals to various disease conditions. We are investigating the effects of NS-SNP mutations on receptor functions in order to provide insights into the disease mechanisms (Roger et al., 2011; Bradley et al., 2011; Jiang et al., 2013).

We were the first to report brilliant blue G (BBG) as a P2X7 antagonist (Jiang et al., 2000), which has been widely-used as a research tool that unravels numerous previously unknown roles for the P2X7 receptor. Our collaborative research identified emodin, a natural compound, as a selective P2X7 antagonist and showed that it was effective in inhibiting cancer cell metastasis (Liu et al., 2010; Jelassi et al., 2013). Recently, we have reported identification of the first novel P2X7 antagonists using a structure-based approach (Caseley et al., 2016).

ATP-induced calcium signalling in mesenchymal stem cell functions

Mesenchymal stem cells (MSCs) exhibit a capacity of osteogenesis, adipogenesis, chondrogenesis and differentiation along other lineages, and hold great promise in cell-based therapies and tissue regeneration. The extrinsic and intrinsic signals and associated signalling mechanisms that control or regulate MSC proliferation, migration and differentiation are still poorly understood.  We are interested in identifying such extrinsic signalling molecules and related intrinsic mechanisms in MSCs. Our recent study shows that purinergic and store-operated Ca2+ signalling mechanisms play an important role in ATP-induced stimulation of MSC migration (Peng et al., 2016; Jiang et al., 2017). We are also interested in exploring the translational potential of manipulating such signalling mechanisms in tissue engineering.

Our research has been supported by grants from BBSRC, Wellcome Trust, Royal Society, and Alzheimer Research Trust; scholarships to talented postgraduate students from BBSRC, EPSRC, Wellcome Trust, University of Leeds, Chinese Scholar Council (CSC) and other governmental agencies; and collaborations with scientists in diverse disciplines in and outside Leeds.

Selective recent publications (IF: journal impact factor published by Clarivate Analytics in 2017; *corresponding author)

[1] Ye, M., Yang, W., Ainscough, J. F., Hu, X., Sedo, A., Zhang, X., Zhang, X., Chen, Z., Li, X., Beech, D. J., Sivaprasadarao, A., Luo, J.* and Jiang, L.-H.* (2014) TRPM2 channel deficiency prevents delayed cytosolic Zn2+ accumulation and CA1 pyramidal neuronal death after transient global ischemia. Cell Death & Disease 5: e1541 (IF5.965)

[2] Zhang, Q.Y., Zhang, Y.Y., Xie, J., Li, C.X., Chen, W.Y., Liu, B.L., Wu, X.A., Li, S.N., Huo, B., Jiang, L.-H.* and Zhao, H.C.* (2014) Stiff substrates enhance cultured neuronal network activity. Scientific Reports 4: 6215 (IF4.259)

[3] Roger, S., Jelassi, B., Couillin, I., Pelegrin, P., Besson, P. and Jiang, L-H. (2015) Understanding the roles of the P2X7 receptor in solid tumour progression and therapeutic perspectives.  BBA Biomembranes 1848: 2584-602 (IF3.498)

[4] Manna, P. T., Munsey, T., Abuarab, N., Li, F., Asipu, A., Howell, G., Sedo, A., Yang, W., Naylor, J., Beech, D.J., Jiang, L.-H.  and Sivaprasadarao, A. (2015) TRPM2 mediated intracellular Zn2+ release triggers pancreatic beta cell death. Biochemical Journal 466: 537-46 (IF3.797)

[5] Jiang, L.-H. (2015) HIV drug nucleoside reverse transcriptase inhibitors as promising anti-inflammation therapeutics by targeting P2X7-dependent large pore formation: one stone for two birds? Frontiers in Pharmacology 6: 38 (IF4.4)

[6] Li, C.-K., Meng, L., Li, X., Li D.-L. and Jiang, L.-H.* (2015) Non-NMDAR neuronal Ca2+-permeable channels in transient ischemia-induced neuronal death as therapeutic targets for ischemic stroke. Expert Opinion On Therapeutic Targets19: 879-92 (IF4.873)

[7] Syed Mortadza, S.A.,  Wang, L.,  Li, D.-L. and Jiang, L.-H.* (2015) TRPM2 channel-mediated ROS-sensitive Ca2+ signaling mechanisms in immune cells. Frontiers in Immunology 6: 407 (IF6.429)

[8] Yu, P., Li, I., Jiang, J., Zhao, Z., Hui, Z., Zhang, J., Zheng, Y., Ling, D., Wang, L., Jiang, L.-H., Luo, J., Zhu, X. and Yang, W. (2015) A dual role of transient receptor potential melastatin 2 channel in cytotoxicity induced by silica nanoparticles. Scientific Report 5: 18171 (IF4.249)

[9] Wei, L.Y., Caseley, E.A., Li, D.L. and Jiang, L.-H.* (2016) ATP-induced P2X receptor-dependent large pore formation: how much do we know? Frontiers in Pharmacology 7: 5 (IF4.4)

[10] Peng, H.S., Hao, Y.J., Mousawi,F., Roger, S., Li, J., Sim, J. A., Ponnambalam, S., Yang, X.B. and Jiang, L.-H.* (2016) Purinergic and store-operated Ca2+ signalling mechanisms in mesenchymal stem cells and their roles in ATP-induced stimulation of cell migration. Stem Cells 34: 2102-2114 (IF5.599)

[11] Xu, F., Wu, X., Jiang, L.-H., Zhao, H. and Pan, J. (2016) An organelle K+ channel is required for osmoregulation in Chlamydomonas reinhardtii. Journal of Cell Science 129: 3008-3014 (IF4.431)

[12] Caseley, E.A., Muench, S.P., Fishwick, C.W. and Jiang, L.-H.* (2016) Structure-based identification and characterisation of structurally novel human P2X7 receptor antagonists. Biochemical Pharmacology 116:130-139 (IF4.581)

[13] Jiang, L.-H.*, Hao, Y.J., Mousawi, F., Peng, H.S. and Yang, X.B. (2017) Expression of P2 purinergic receptors in mesenchymal stem cells and their roles in extracellular nucleotide regulation of cell functions. Journal of Cell Physiology 232: 287-297 (IF4.08) 

[14] Yu, P., Xue, X., Zhang, J., Hu, X., Wu, Y., Jiang, L.-H., Jin, H., Luo, J., Zhang, L., Liu, Z. and Yang, W. (2017) Identification of the ADPR binding pocket in the NUDT9 homology domain of TRPM2. Journal of General Physiology 149: 219-235 (IF4.2)

[15] Syed Mortadza, S., Sim, J.A., Stacey, M. and Jiang, L.-H.* (2017) Signalling mechanisms mediating Zn2+-induced TRPM2 channel activation and death cell in microglial cells. Scientific Reports 7: 45032 (IF4.529)

[16] Zhao, H., Yu, Y., Wu, X., Liu, S., Liu, B., Du, J., Li, B., Jiang, L* and Feng, X.* (2017) A role of BK channel in regulation of Ca2+ channel in ventricular myocytes by substrate stiffness. Biophysical Journal 112: 1406-1416 (IF3.656)

[17] Jiang, Q., Gao, Y., Wang, C., Liao, M., Wu, Y., Zhan, K., Lu, N., Tao, R., Lu, Y., Wilcox, C.S., Luo, J., Jiang, L.-H.*, Yang, W.* and Han, F.* (2017) Nitration of TRPM2 as a molecular switch induces autophagy during brain pericyte injury. Antioxidants and Redox Signaling (in press) (IF6.337) 

[18] Khalid, M., Brisson, L., Tariq, M., Hao, H.J., Guibon, R., Fromont, G., Syed Mortadza, S.A., Mousawi, F., Manzoor, S.*, Roger, S.* and Jiang, L.-H.* (2017) Carcinoma-specific expression of P2Y11 receptor and its contribution in ATP-induced purinergic signalling and cell migration in human hepatocellular carcinoma cells. Oncotarget 8:37278-37290 (IF5.1688) 

[19] Jiang, L.-H.*, Mousawi, F., Yang, X.B. and Roger, S. (2017) ATP-induced Ca2+-signalling mechanisms in the regulation of mesenchymal stem cell migration Cellular and Molecular Life Sciences (in press) (IF5.788)

Lab alumni

[1] Zhuzhong Mei (2004-2007), Research fellow

[2] Rong Xia (2005-2009), PhD student; a recipient of Chinese Government Award for Outstanding Self-Financed Student Abroad in 2008

[3] Hongju Mao (2005-2006), Research fellow

[4] Xing Liu (2006-2009), Research fellow 

[5] Helen J Bradley (2006-2010), PhD student

[6] Wei Yang (2009-2011), Research fellow

[7] Jie Zou (2009-2013), PhD student 

[8] Hongsen Peng (2010-2014), PhD student 

[9] Yunjie Hao (2014-2015), MSc by Research student

[10] Emily A Caseley (2013-2016), PhD student 

 

Faculty Research and Innovation



Studentship information

See also:

Modules managed

BMSC2232 - Topics in Physiology
BMSC3140 - Advanced Scientific Skills

Modules taught

BIOL5171M - Infectious & Non-infectious Diseases
BIOW5906X - Theory, computation and bioinformatics
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2120 - Scientific Skills
BMSC2223 - Neuropharmacology
BMSC2232 - Topics in Physiology
BMSC3101 - Inherited Disorders
BMSC3140 - Advanced Scientific Skills
BMSC3143/44/45/46 - Advanced topics for BMS students I
BMSC3301 - Research Project in Biomedical Sciences
DSUR5072M - Stem Cell Therapy and Tissue Engineering

Committees

Member of Faculty Taught Student Education Committee (Co-opted member)

Centre membership: Neuroscience Research at Leeds (NeuR@L)

Postgraduates

John Brewster (Primary supervisor) 60% FTE
Emily Caseley (Primary supervisor) 50% FTE
Xin Li (Primary supervisor) 75% FTE
Harneet Mankoo (Primary supervisor) 100% FTE
Fatema Mousawi (Primary supervisor) 70% FTE
Sharifah Alawieyah Syed Mortadza (Primary supervisor) 75% FTE
Adam Hyman (Co-supervisor) 20% FTE
Kein Lon Man (Co-supervisor) 15% FTE
Daniel Secker (Co-supervisor) 20% FTE

Jiang LH, Mousawi F, Yang X, Roger S ATP-induced Ca<sup>2+</sup>-signalling mechanisms in the regulation of mesenchymal stem cell migration Cellular and Molecular Life Sciences 1-14, 2017
DOI:10.1007/s00018-017-2545-6
View abstract

Zhao H, Yu Y, Wu X, Liu S, Liu B, Du J, Li B, Jiang L, Feng X A Role of BK Channel in Regulation of Ca2+ Channel in Ventricular Myocytes by Substrate Stiffness BIOPHYSICAL JOURNAL 112 1406-1416, 2017
DOI:10.1016/j.bpj.2017.01.036

Mortadza SS, Sim JA, Stacey M, Jiang LH Signalling mechanisms mediating Zn 2+ -induced TRPM2 channel activation and cell death in microglial cells Scientific Reports 7 -, 2017
DOI:10.1038/srep45032
View abstract

Caseley EA, Muench SP, Jiang LH Conformational changes during human P2X7 receptor activation examined by structural modelling and cysteine-based cross-linking studies Purinergic Signal 13 135-141, 2017
DOI:10.1007/s11302-016-9553-0
View abstract

Barniol-Xicota M, Kwak SH, Lee SD, Caseley E, Valverde E, Jiang LH, Kim YC, Vázquez S Escape from adamantane: Scaffold optimization of novel P2X7 antagonists featuring complex polycycles Bioorganic and Medicinal Chemistry Letters 27 759-763, 2017
DOI:10.1016/j.bmcl.2017.01.039
View abstract

Jiang LH, Hao Y, Mousawi F, Peng H, Yang X Expression of P2 Purinergic Receptors in Mesenchymal Stem Cells and Their Roles in Extracellular Nucleotide Regulation of Cell Functions Journal of Cellular Physiology 232 287-297, 2017
DOI:10.1002/jcp.25484
View abstract

Yu P, Xue X, Zhang J, Hu X, Wu Y, Jiang LH, Jin H, Luo J, Zhang L, Liu Z, Yang W Identification of the ADPR binding pocket in the NUDT9 homology domain of TRPM2 Journal of General Physiology 149 219-235, 2017
DOI:10.1085/jgp.201611675
View abstract

Khalid M, Brisson L, Tariq M, Hao Y, Guibon R, Fromont G, Syed Mortadza SA, Mousawi F, Manzoor S, Roger S, Jiang LH Carcinoma-specific expression of P2Y<inf>11</inf>receptor and its contribution in ATP-induced purinergic signalling and cell migration in human hepatocellular carcinoma cells Oncotarget 8 37278-37290, 2017
DOI:10.18632/oncotarget.16191
View abstract

Caseley EA, Muench SP, Fishwick CW, Jiang LH Structure-based identification and characterisation of structurally novel human P2X7 receptor antagonists Biochemical Pharmacology 116 130-139, 2016
DOI:10.1016/j.bcp.2016.07.020
View abstract

Xu F, Wu X, Jiang L-H, Zhao H, Pan J An organelle K+ channel is required for osmoregulation in Chlamydomonas reinhardtii. J Cell Sci 129 3008-3014, 2016
DOI:10.1242/jcs.188441
View abstract

Wei L, Caseley E, Li D, Jiang L-H ATP-induced P2X Receptor-Dependent Large Pore Formation: How Much Do We Know? Frontiers in Pharmacology 7 -, 2016
DOI:10.3389/fphar.2016.00005

Peng H, Hao Y, Mousawi F, Roger S, Li J, Sim JA, Ponnambalam S, Yang X, Jiang LH Purinergic and Store-Operated Ca<sup>2+</sup>Signaling Mechanisms in Mesenchymal Stem Cells and their Roles in ATP-Induced Stimulation of Cell Migration Stem Cells -, 2016
DOI:10.1002/stem.2370
View abstract

Yu P, Li J, Jiang J, Zhao Z, Hui Z, Zhang J, Zheng Y, Ling D, Wang L, Jiang LH, Luo J, Zhu X, Yang W A dual role of transient receptor potential melastatin 2 channel in cytotoxicity induced by silica nanoparticles Scientific Reports 5 -, 2015
DOI:10.1038/srep18171
View abstract

Roger S, Jelassi B, Couillin I, Pelegrin P, Besson P, Jiang L-H Understanding the roles of the P2X7 receptor in solid tumour progression and therapeutic perspectives BBA: Biomembranes 1848 2584-2602, 2015
DOI:10.1016/j.bbamem.2014.10.029
View abstract

Caseley EA, Muench SP, Baldwin SA, Simmons K, Fishwick CW, Jiang L-H Docking of competitive inhibitors to the P2X7 receptor family reveals key differences responsible for changes in response between rat and human Bioorganic and Medicinal Chemistry Letters 25 3164-3167, 2015
DOI:10.1016/j.bmcl.2015.06.001
View abstract

Manna PT, Munsey TS, Abuarab N, Li F, Asipu A, Howell G, Sedo A, Yang W, Naylor J, Beech DJ, Jiang LH, Sivaprasadarao A TRPM2-mediated intracellular Zn&lt;sup&gt;2+&lt;/sup&gt; release triggers pancreaticβ-cell death Biochemical Journal 466 537-546, 2015
DOI:10.1042/BJ20140747
View abstract

Li C, Meng L, Li X, Li D, Jiang L-H Non-NMDAR neuronal Ca2+-permeable channels in delayed neuronal death and as potential therapeutic targets for ischemic brain damage EXPERT OPINION ON THERAPEUTIC TARGETS 19 879-892, 2015
DOI:10.1517/14728222.2015.1021781

Jiang LH HIV drug nucleoside reverse transcriptase inhibitors as promising anti-inflammation therapeutics by targeting P2X7-dependent large pore formation: one stone for two birds? Frontiers in Pharmacology 6 -, 2015
DOI:10.3389/fphar.2015.00038

Mortadza SAS, Wang L, Li D, Jiang LH TRPM2 channel-mediated ROS-sensitive Ca&lt;sup&gt;2+&lt;/sup&gt; signaling mechanisms in immune cells Frontiers in Immunology 6 -, 2015
DOI:10.3389/fimmu.2015.00407
View abstract

Ye M, Yang W, Ainscough JF, Hu X-P, Li X, Sedo A, Zhang X-H, Zhang X, Chen Z, Li X-M, Beech DJ, Sivaprasadarao A, Luo J-H, Jiang L-H TRPM2 channel deficiency prevents delayed cytosolic Zn<sup>2+</sup>accumulation and CA1 pyramidal neuronal death after transient global ischemia Cell Death and Disease 5 -, 2014
DOI:10.1038/cddis.2014.494
View abstract

Yu W, Jiang L-H, Zheng Y, Hu X, Luo J, Yang W Inactivation of TRPM2 channels by extracellular divalent copper PLoS ONE 9 -, 2014
DOI:10.1371/journal.pone.0112071
View abstract

Zhang Q-Y, Zhang Y-Y, Xie J, Li C-X, Chen W-Y, Liu B-L, Wu X-A, Li S-N, Huo B, Jiang L-H, Zhao H-C Stiff substrates enhance cultured neuronal network activity Scientific Reports 4 -, 2014
DOI:10.1038/srep06215
View abstract

Caseley EA, Muench SP, Roger S, Mao H-J, Baldwin SA, Jiang L-H Non-synonymous single nucleotide polymorphisms in the P2X receptor genes: Association with diseases, impact on receptor functions and potential use as diagnosis biomarkers International Journal of Molecular Sciences 15 13344-13371, 2014
DOI:10.3390/ijms150813344
View abstract

Zou J, Ainscough JF, Yang W, Sedo A, Yu S-P, Mei Z-Z, Sivaprasadarao A, Beech DJ, Jiang L-H A differential role of macrophage TRPM2 channels in Ca2+ signaling and cell death in early responses to H2O2 American Journal of Physiology: Cell Physiology 305 C61-C69, 2013
DOI:10.1152/ajpcell.00390.2012
View abstract

Jelassi B, Anchelin M, Chamouton J, Cayuela ML, Clarysse L, Li J, Goré J, Jiang L-H, Roger S Anthraquinone emodin inhibits human cancer cell invasiveness by antagonizing P2X7 receptors Carcinogenesis 34 1487-1496, 2013
DOI:10.1093/carcin/bgt099
View abstract

Yang W, Jiang L-H Site-directed mutagenesis to study the structure-function relationships of ion channels., 2013
DOI:10.1007/978-1-62703-351-0_20
View abstract

Jiang L-H, Baldwin JM, Roger S, Baldwin SA Insights into the Molecular Mechanisms Underlying Mammalian P2X7 Receptor Functions and Contributions in Diseases, Revealed by Structural Modeling and Single Nucleotide Polymorphisms. Front Pharmacol 4 55-, 2013
DOI:10.3389/fphar.2013.00055
View abstract

Jiang L-H Cysteine-based cross-linking approach to study inter-domain interactions in ion channels., 2013
DOI:10.1007/978-1-62703-351-0_21
View abstract

Jiang LH P2X receptor-mediated ATP purinergic signaling in health and disease Cell Health and Cytoskeleton 4 83-101, 2012
DOI:10.2147/CHC.S27196
View abstract

Xia R, Samad TA, Btesh J, Jiang LH, Kays I, Stjernborg L, Dekker N TRPV1 Signaling: Mechanistic Understanding and Therapeutic Potential CURR TOP MED CHEM 11 2180-2191, 2011

Bradley HJ, Browne LE, Yang W, Jiang L-H Pharmacological properties of the rhesus macaque monkey P2X7 receptor. Br J Pharmacol 164 743-754, 2011
DOI:10.1111/j.1476-5381.2011.01399.x
View abstract

Zou J, Yang W, Beech DJ, Jiang L-H A residue in the TRPM2 channel outer pore is crucial in determining species-dependent sensitivity to extracellular acidic pH. Pflugers Arch 462 293-302, 2011
DOI:10.1007/s00424-011-0957-y
View abstract

Yang W, Manna PT, Zou JE, Luo JH, Beech DJ, Sivaprasadarao A, Jiang LH Zinc Inactivates Melastatin Transient Receptor Potential 2 Channels via the Outer Pore J BIOL CHEM 286 23789-23798, 2011
DOI:10.1074/jbc.M111.247478

Jiang LH, Gamper N, Beech DJ Properties and Therapeutic Potential of Transient Receptor Potential Channels with Putative Roles in Adversity: Focus on TRPC5, TRPM2 and TRPA1 CURR DRUG TARGETS 12 724-736, 2011

Bradley HJ, Baldwin JM, Goli GR, Johnson B, Zou J, Sivaprasadarao A, Baldwin SA, Jiang LH Residues 155 and 348 Contribute to the Determination of P2X(7) Receptor Function via Distinct Mechanisms Revealed by Single-nucleotide Polymorphisms J BIOL CHEM 286 8176-8187, 2011
DOI:10.1074/jbc.M110.211284

Bradley HJ, Liu X, Collins V, Owide J, Goli GR, Smith M, Surprenant A, White SJ, Jiang LH Identification of an intracellular microdomain of the P2X(7) receptor that is crucial in basolateral membrane targeting in epithelial cells FEBS LETT 584 4740-4744, 2010
DOI:10.1016/j.febslet.2010.11.007

Yang W, Zou J, Xia R, Vaal ML, Seymour VA, Luo JH, Beech DJ, Jiang LH State-dependent Inhibition of TRPM2 Channel by Acidic pH J BIOL CHEM 285 30411-30418, 2010
DOI:10.1074/jbc.M110.139774

Jiang LH, Yang W, Zou J, Beech DJ TRPM2 channel properties, functions and therapeutic potentials EXPERT OPIN THER TAR 14 973-988, 2010
DOI:10.1517/14728222.2010.510135

Liu LJ, Zou J, Liu X, Jiang LH, Li JY Inhibition of ATP-induced macrophage death by emodin via antagonizing P2X(7) receptor EUR J PHARMACOL 640 15-19, 2010
DOI:10.1016/j.ejphar.2010.04.036

Li Q, Sun BY, Wang XF, Jin Z, Zhou Y, Dong L, Jiang LH, Rong WF A Crucial Role for Hydrogen Sulfide in Oxygen Sensing via Modulating Large Conductance Calcium-Activated Potassium Channels ANTIOXID REDOX SIGN 12 1179-1189, 2010
DOI:10.1089/ars.2009.2926

Browne LE, Jiang LH, North RA New structure enlivens interest in P2X receptors TRENDS PHARMACOL SCI 31 229-237, 2010
DOI:10.1016/j.tips.2010.02.004

Roger S, Mei ZZ, Baldwin JM, Dong L, Bradley H, Baldwin SA, Surprenant A, Jiang LH Single nucleotide polymorphisms that were identified in affective mood disorders affect ATP-activated P2X(7) receptor functions J PSYCHIATR RES 44 347-355, 2010
DOI:10.1016/j.jpsychires.2009.10.005

Li DL, Liu X, Xia R, Ross C, Yang XB, Jiang LH Pharmacological properties of ATP-sensitive purinergic receptors expressed in human G292 osteoblastic cells EUR J PHARMACOL 617 12-16, 2009
DOI:10.1016/j.ejphar.2009.06.049

Mei ZZ, Jiang LH Requirement for the N-Terminal Coiled-Coil Domain for Expression and Function, but not Subunit Interaction of, the ADPR-Activated TRPM2 Channel J MEMBRANE BIOL 230 93-99, 2009
DOI:10.1007/s00232-009-9190-4

Jiang LH Inhibition of P2X(7) receptors by divalent cations: old action and new insight EUR BIOPHYS J BIOPHY 38 339-346, 2009
DOI:10.1007/s00249-008-0315-y

Liu X, Ma W, Surprenant A, Jiang LH Identification of the amino acid residues in the extracellular domain of rat P2X(7) receptor involved in functional inhibition by acidic pH BRIT J PHARMACOL 156 135-142, 2009
DOI:10.1111/j.1476-5381.2008.00002.x

Milligan CJ, Li J, Sukumar P, Majeed Y, Dallas ML, English A, Emery P, Porter KE, Smith AM, McFadzean I, Beccano-Kelly D, Bahnasi Y, Cheong A, Naylor J, Zeng FN, Liu X, Gamper N, Jiang LH, Pearson HA, Peers C, Robertson B, Beech DJ Robotic multiwell planar patch-clamp for native and primary mammalian cells NAT PROTOC 4 244-255, 2009
DOI:10.1038/nprot.2008.230

Young MT, Zhang YH, Cao LS, Broomhead H, Jiang LH Role of the domain encompassing Arg(304)-Ile(328) in rat P2X(2) receptor conformation revealed by alterations in complex glycosylation at Asn(298) BIOCHEM J 416 137-143, 2008
DOI:10.1042/BJ20081182

Li J, Sukumar P, Milligan CJ, Kumar B, Ma ZY, Munsch CM, Jiang LH, Porter KE, Beech DJ Interactions, functions, and independence of plasma membrane STIM1 and TRPC1 in vascular smooth muscle cells CIRC RES 103 E97-U311, 2008
DOI:10.1161/CIRCRESAHA.108.182931

Xia R, Mei ZZ, Mao HJ, Yang W, Dong L, Bradley H, Beech DJ, Jiang LH Identification of pore residues engaged in determining divalent cationic permeation in transient receptor potential melastatin subtype channel J BIOL CHEM 283 27426-27432, 2008
DOI:10.1074/jbc.M801049200

Xia R, Mei ZZ, Milligan C, Jiang LH Inhibitory interaction between P2X(4) and GABA(C) rho 1 receptors BIOCHEM BIOPH RES CO 375 38-43, 2008
DOI:10.1016/j.bbrc.2008.07.096

Xu SZ, Sukumar P, Zeng FN, Li J, Jairaman A, English A, Naylor J, Ciurtin C, Majeed Y, Milligan CJ, Bahnasi YM, Al-Shawaf E, Porter KE, Jiang LH, Emery P, Sivaprasadarao A, Beech DJ TRPC channel activation by extracellular thioredoxin NATURE 451 69-U9, 2008
DOI:10.1038/nature06414

Liu X, Surprenant A, Mao HJ, Roger S, Xia R, Bradley H, Jiang LH Identification of key residues coordinating functional inhibition of P2X(7) receptors by zinc and copper MOL PHARMACOL 73 252-259, 2008

Jiang LH Subunit interaction in channel assembly and functional regulation of transient receptor potential melastatin (TRPM) channels BIOCHEM SOC T 35 86-88, 2007
View abstract

Mei ZZ, Xia R, Beech DJ, Jiang LH Intracellular coiled-coil domain engaged in subunit interaction and assembly of melastatin-related transient receptor potential channel 2 J BIOL CHEM 281 38748-38756, 2006
DOI:10.1074/jbc.M607591200

Stokes L, Jiang LH, Alcaraz L, Bent J, Bowers K, Fagura M, Furber M, Mortimore M, Lawson M, Theaker J, Laurent C, Braddock M, Surprenant A Characterization of a selective and potent antagonist of human P2X(7) receptors, AZ11645373 BRIT J PHARMACOL 149 880-887, 2006
DOI:10.1038/sj.bjp.0706933

Mei ZZ, Mao HJ, Jiang LH Conserved cysteine residues in the pore region are obligatory for human TRPM2 channel function AM J PHYSIOL-CELL PH 291 C1022-C1028, 2006
DOI:10.1152/ajpcell.00606.2005

Wilkinson WJ, Jiang LH, Surprenant A, North RA Role of ectodomain lysines in the subunits of the heteromeric P2X(2/3) receptor MOL PHARMACOL 70 1159-1163, 2006
DOI:10.1124/mol.106.026658

Jiang LH, Rassendren F, Mackenzie A, Zhang YH, Surprenant A, North RA N-methyl-D-glucamine and propidium dyes utilize different permeation pathways at rat P2X(7) receptors AM J PHYSIOL-CELL PH 289 C1295-C1302, 2005
DOI:10.1152/ajpcell.00253.2005

Chaumont S, Jiang LH, North RA, Rassendren F Identification of a trafficking motif involved in the stabilization and polarization of P2X receptors Journal of Biological Chemistry 279 29628-29638, 2004
DOI:10.1074/jbc.M403940200
View abstract

Jiang LH, Kim M, Spelta V, Bo X, Surprenant A, North RA Subunit arrangement in P2X receptors Journal of Neuroscience 23 8903-8910, 2003

Bo X, Jiang LH, Wilson HL, Kim M, Burnstock G, Surprenant A, North RA Pharmacological and Biophysical Properties of the Human P2X<sub>5</sub>Receptor Molecular Pharmacology 63 1407-1416, 2003
DOI:10.1124/mol.63.6.1407

Spelta V, Jiang LH, Bailey RJ, Suprenant A, North RA Interaction between cysteines introduced into each transmembrane domain of the rat P2X<sub>2</sub>receotor. British Journal of Pharmacology 138 131-136, 2003
DOI:10.1038/sj.bjp.0705018

Spelta V, Jiang LH, Suprenant A, North RA Kinetics of antagonists at P2X<sub>2/3</sub>receptors. British Journal of Pharmacology 135 1524-1530, 2002
DOI:10.1038/sj.bjp.0704591
View abstract

Kim M, Jiang LH, Wilson HL, North RA, Suprenant A Proteomic and functional evidence for a P2X<sub>7</sub>receptor signalling complex EMBO Journal 20 6347-6358, 2001
DOI:10.1093/emboj/20.22.6347
View abstract

Jiang LH, Rassendren F, Spelta V, Suprenant A, North RA Amino Acid Residues Involved in Gating Identified in the First Membrane-spanning Domain of the Rat P2X<sub>2</sub>Receptor Journal of Biological Chemistry 276 14902-14908, 2001
DOI:10.1074/jbc.M011327200
View abstract

Jiang LH, Rassendren F, Spelta V, Suprenant A, North RA Identification of amino acid residues contributing to the ATP binding site of a purinergic P2X receptor. Journal of Biological Chemistry 275 34190-34196, 2000
View abstract

Jiang LH, MacKenzie AB, North RA, Suprenant A Brilliant Blue G selectively blocks ATP-gated P2X<sub>7</sub>receptors Molecular Pharmacology 58 82-88, 2000
View abstract

Jiang LH, Gawler DJ, Hodson N, Milligan CJ, Pearson HA, Porter V, Wray D Regulation of cloned cardiac L-type calcium channels by cGMP-dependent protein kinase Journal of Biological Chemistry 275 6135-6143, 2000
View abstract

Jiang Q, Gao Y, Wang C, Tao R-R, Wu Y, Zhan K, Liao M-H, Lu N, Lu Y-M, Wilcox CS, Luo J, Jiang L-H, Yang W, Han F Nitration of TRPM2 as a Molecular Switch Induces Autophagy during Brain Pericyte Injury Antioxid Redox Signal -,
DOI:10.1089/ars.2016.6873
View abstract