Faculty of Biological Sciences

Dr Lars Jeuken

PhD 2001
Reader in Biophysics
School of Biomedical Sciences

Contact:  Garstang Bioincuabtor 7.31, +44(0) 113 34 33829, email address for  

You can read more about Dr Jeuken's interests here:
http://www.fbs.leeds.ac.uk/jeukengroup/


Research Interests

Redox-active membrane proteins, biological electron transfer

Our aim is to to control the interaction between biological macromolecules and inorganic 'solid' surfaces. The interaction between biomolecules and surfaces is key to many assays, but often not controlled. Take for instance an ELISA assay; Biomolecules are 'randomly' physisorbed on plastic 96-well plates without any control on the orientation of these molecules. However, biomolecules for which the epitope is orientated towards the plastic surfaces might not interact with antibodies. The interaction between solid, inorganic surfaces is particular important for membrane proteins, where the hydrophobic regions of the protein will change the way the proteins interacts with its environment. Vice versa, the influence of inorganic surfaces on proteins might be more pronounces for membrane proteins, denaturing the protein or otherwise influencing the function of the membrane proteins. Membrane proteins, in general, are more difficult to study due to their amphiphatic nature. Many membrane enzymes are studied in detergent solutions or only hydrophilic subunits are used (i.e., removing the integral membrane subunits). However, the lipid membrane has an important influence on the proteins that reside in them and experimental techniques that do not include the membrane are thus limited. When studying membrane proteins with assays that include their adsorption to a solid surface, one would ideally like to retain a native-like lipid environment. Important examples are enzymes that interact with the hydrophobic quinone pool (like ubiquinol oxidase). Other examples include 'membrane-bound hydrogenases', where the majority of studies are performed on the two hydrophilic subunits, without the third transmembrane quinone-converting subunit.

Within Leeds

Our research on membrane proteins is facilitated by colleagues in the University wide Integrative Membrane Biology group.

Our work is highly interdisciplinary in nature, which is greatly facilitated by various collaboration within the Structural Molecular Biology group.

 

Current Projects

Redox-active membrane enzymes

We have modified electrodes with lipid membranes in which the membranes either adopt a planar geometry (see Figure) or liposomes are immobilised intact on the surface. This allows us to study redox-active membrane proteins in their native environment. We use these systems to study the catalytic activity and proton pumping activity of an ubiquinol oxidase from E. coli, cytochrome bo3. Currently, we have three projects in this area. 

Single-enzyme studies of a proton pump

By modifying the electrode surfaces, membrane vesicles can be adsorbed on the surface that stay intact. By loading these vesicles with fluorescent probes we can simultaneously control the activity of the ubiquinol oxidase, cytochrome bo3 (using electrochemical methods) and measure its proton pumping activity (see also the ERC project 'MEME'). Currently, this experimental setup is used to study the proton pumping activity of a heme-copper oxidase at the single-enzyme level.

Respiration in Shewanella oneidensis MR-1

S. oneidensis is a proteobacterium and gram-negative facultative-anaerobe that has a remarkably diverse multicomponent and branched electron transport chain, making it an important model organism for bioreactor, bioengineering and bioremediation studies. Its respiratory plasticity allows S. oneidensis to link proton translocation to the reduction of a wide variety of electron acceptors such as O2, fumarate, nitrate, arsenate, DMSO, TMAO, Fe(III), Mn(IV), Cr(VI), V(V) oxides and various forms of other carbonaceous and sulfur-based compounds. During anearobic respiration, the quinone pool is oxidised by a single enzyme, CymA, which transfers the electrons to one of the many terminal reducases. We have used our membrane-modified electrodes to study QH2/Q oxidoreductases activity of CymA and its partners proteins in the periplasm (see Figure). S. oneidensis is also able to respire on electrode surfaces, transferring electrons extracellularly via MtrCAB, which span the periplasm and outer membrane. Electron transfer throught the MtrCAB complex and CymA is characterised in our lab using the membrane modified electrodes.

Membrane-bound hydrogenase (MBH)

MBHs are hydrogen/proton converting enzymes that donate to or extract electrons from the quinol pool in the lipid membrane. These enzymes are extensively studied using electrochemical methods, but invariably only the two hydrophilic subunits are used, omitting a third membrane subunit that interacts with the quinone pool. In this project, the whole heterotrimeric MBH is placed in the planar lipid membrane on the electrode surface (see image above) and the activity and other properties (such is deactivation) are characterised using electrochemical methods. 

Interactions between nanoparticles/nanowires and cell membranes

Exploiting Transmembrane Cytochromes for Solar Energy Conversion

There is vast potential for renewable fuel and electricity generation through the photochemical conversion of solar to chemical energy. We wish to tap into what is a largely unused power supply and develop the basic principles for a novel biotechnology that will harness solar energy using a biological synthetic approach, in which a bacterial respiratory machinery is coupled to inorganic photosensitisers. Our biosynthetic approach is inspired by plant photosynthesis where solar energy is absorbed by the special pair of chlorophylls (the photosensitisers) and, ultimately, converted to chemical energy. Our ambition is to overcome the major drawbacks of most artificial homogenous photosynthetic systems: a short-lived charge separated state, which is due to the failure to spatially decouple the reductive and the oxidative sites required to sustain charge separation. In plant photosynthesis, the latter is achieved by rapid transmembrane electron transfer. In this project, which is a collaboration with the Universities of East Anglia and Cambridge, transmembrane charge separation is achieved by exploiting the membrane-spanning electron-transfer conduits of the microbe Shewanella oneidensis MR-1, where the cytochrome conduits play an integral part of their ability to respire on inorganic minerals. As a proof of principle, we aim to photocatalyse the reduction of protons to hydrogen, using both inorganic (platinum) and biological (hydrogenase) catalysts. The resulting photocatalyst adopts the principles of natural photosynthesis (light harvesting, charge separation and catalysis) in a device that spatially separates the sites of photoexcitation and oxidation to that from reduction (hydrogen evolution). The image illustrates a cytochrome condiut protein (MtrF) coupled to a inorganic photosensitiser (TiO2). The assembly is adorbed on a gold electrode and electrochemistry is used to characterise the electron transfer rates between (photoexcited) TiO2 and MtrF.

Microbial fuel cells

Recently, an intense interest has emerged in microorganisms that generate electrical current from organic matter in microbial fuel cells (MFCs). MFCs have the ability to generate a clean and renewable energy and to generate continuous power in specialised environments like the bottom of the sea bed or the human body. However, one of the greatest bottlenecks in MFC development is their limited power output, which is still insufficient to drive most electronic devices in society. The limitation in power output is mostly due to the slow rate of electron transfer from the microbes to the anode of the MFC. Using our technology, we aim to 'electrically' connect bacteria in biofilms to the electrode (anode) via nanowires and thereby enhancing the electron transfer rate between microbe and the electrode, and thus the power output of the MFC. To analyse the interactions between Shewanella bacteria and chemically-modified nanoparticles, we make use of quantum dots, which are semi-conducting nanoparticulates that are strongly fluorescent and thus easy to image (see Figure).

Spherical-supported bilayer lipid membranes (ssBLM)

Nanoparticles can be relatively easily extracted from (aqueous) solution via (low-speed) centrifugation or, in the case of (para)magnetic particles, with a magnet. In our lab we coat silica nanoparticles with lipid bilayers. By including membrane proteins in the membrane-coated nanoparticles, we create a technological platform in which the membrane proteins can be easily extracted. Sponsored by MedImmune, we are currently exploring this platform for applications in phage display, raising antibody or antibody mimetic proteins against membrane proteins.

Nanotoxicology and nanomedicine

The toxic effects of nanomaterials are poorly understood and their effects on aquatic wildlife are largely unknown. In the absence of such basic toxicological information, it is difficult to set environmental quality standards or perform risk assessments for these materials. In a project funded by the EU, ENNSATOX, this crucial uncertainty was addressed by seeking to relate the structure and functionality of well-known nanoparticles (silica or ZnO) of varying morphology to its biological activity at the membrane level. Our lab focuses particularly on whether nanomaterials are able to induce damage, either mechanically or via the production of radiacal species, and whether nanoparticles are able to passively pass a cell membrane, thereby potentially inducing toxic effect in the cytoplasm. Alterantively, by creating nanomaterials that can passively pass the membrane without too many toxic concequences, a drug delivery system can be designed.

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • 1) Artificial photosynthesis
  • 2) Transport of metabolites and drugs across the cell membrane
  • 3) Nanotoxicology (group project)

Postgraduate studentship areas:

  • We have various funded PhD positions available throughout the year.

See also:

Modules managed

BIOL2210 - Biological Membranes and Cell Signalling
BIOL5262M - Molecular Diagnostics and Drug Delivery

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC2303 - Intermediate Biochemistry: Skills
BIOL2210/BIOC2301 - Integrated Biochemistry/Biological Membranes
BIOL3398 - Research Tools and Applications
BIOL3399 - Extended Research Project Preparation
BIOL5262M - Molecular Diagnostics and Drug Delivery
BIOW5906X - Theory, computation and bioinformatics
BMSC1103 - Basic Laboratory and Scientific Skills
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2229 - Experimental Skills in Medical Sciences
BMSC3301 - Research Project in Biomedical Sciences
BMSC3399 - Extended Research Project Preparation

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Dr Lars Jeuken  (Reader in Biophysics)

Redox-active membrane proteins, biological electron transfer 

Miss Hope Adamson  (Research Fellow)

Dr George Heath  (Visiting Research Fellow)

Mrs Honglin Rong  (Research Technician)


Postgraduates

Matthias Gantner (Primary supervisor) 50% FTE
Theodoros Laftsoglou (Primary supervisor) 80% FTE
Joseph Oram (Primary supervisor) 90% FTE
Anna Stikane (Primary supervisor) 67% FTE
Vlad Vasilca (Primary supervisor) 75% FTE
Anna Wroblewska-Wolna (Primary supervisor) 67% FTE
Ruth Craven (Co-supervisor) 33% FTE
Ashley Hancock (Co-supervisor) 10% FTE
Andrew Harvie (Co-supervisor) 20% FTE

Li M, Khan S, Rong H, Tuma R, Hatzakis NS, Jeuken LJC Effects of membrane curvature and pH on proton pumping activity of single cytochrome bo<inf>3</inf>enzymes Biochimica et Biophysica Acta - Bioenergetics 1858 763-770, 2017
DOI:10.1016/j.bbabio.2017.06.003
View abstract

Heath GR, Li M, Rong H, Radu V, Frielingsdorf S, Lenz O, Butt JN, Jeuken LJC Multilayered Lipid Membrane Stacks for Biocatalysis Using Membrane Enzymes Advanced Functional Materials 27 -, 2017
DOI:10.1002/adfm.201606265
View abstract

Laftsoglou T, Jeuken LJC Supramolecular electrode assemblies for bioelectrochemistry Chemical Communications 53 3801-3809, 2017
DOI:10.1039/c7cc01154g
View abstract

Reuillard B, Ly KH, Hildebrandt P, Jeuken LJC, Butt JN, Reisner E High Performance Reduction of H2O2 with an Electron Transport Decaheme Cytochrome on a Porous ITO Electrode Journal of the American Chemical Society 139 3324-3327, 2017
DOI:10.1021/jacs.6b12437
View abstract

Beales PA, Khan S, Muench SP, Jeuken LJC Durable vesicles for reconstitution of membrane proteins in biotechnology Biochemical Society Transactions 45 15-26, 2017
DOI:10.1042/BST20160019
View abstract

Hwang ET, Orchard KL, Hojo D, Beton J, Lockwood CWJ, Adschiri T, Butt JN, Reisner E, Jeuken LJC Exploring Step-by-Step Assembly of Nanoparticle:Cytochrome Biohybrid Photoanodes ChemElectroChem -, 2017
DOI:10.1002/celc.201700030
View abstract

Ainsworth EV, Lockwood CWJ, White GF, Hwang ET, Sakai T, Gross MA, Richardson DJ, Clarke TA, Jeuken LJC, Reisner E, Butt JN Photoreduction of Shewanella oneidensis Extracellular Cytochromes by Organic Chromophores and Dye-Sensitized TiO2. Chembiochem 17 2324-2333, 2016
DOI:10.1002/cbic.201600339
View abstract

Li KM, Wilkinson C, Kellosalo J, Tsai JY, Kajander T, Jeuken LJC, Sun YJ, Goldman A Membrane pyrophosphatases from Thermotoga maritima and Vigna radiata suggest a conserved coupling mechanism Nature Communications 7 -, 2016
DOI:10.1038/ncomms13596

Ye S, Benz F, Wheeler MC, Oram J, Baumberg JJ, Cespedes O, Christenson HK, Coletta PL, Jeuken LJC, Markham AF, Critchley K, Evans SD One-step fabrication of hollow-channel gold nanoflowers with excellent catalytic performance and large single-particle SERS activity Nanoscale 8 14932-14942, 2016
DOI:10.1039/c6nr04045d
View abstract

Lee C-Y, Reuillard B, Sokol KP, Laftsoglou T, Lockwood CWJ, Rowe SF, Hwang ET, Fontecilla-Camps JC, Jeuken LJC, Butt JN, Reisner E A decahaem cytochrome as an electron conduit in protein-enzyme redox processes Chemical Communications 52 7390-7393, 2016
DOI:10.1039/c6cc02721k
View abstract

Oram J, Jeuken LJC A Re-evaluation of Electron-Transfer Mechanisms in Microbial Electrochemistry: Shewanella Releases Iron that Mediates Extracellular Electron Transfer ChemElectroChem 3 829-835, 2016
DOI:10.1002/celc.201500505
View abstract

Wiebalck S, Kozuch J, Forbrig E, Tzschucke CC, Jeuken LJC, Hildebrandt P Monitoring the Transmembrane Proton Gradient Generated by Cytochrome bo<inf>3</inf>in Tethered Bilayer Lipid Membranes Using SEIRA Spectroscopy Journal of Physical Chemistry B 120 2249-2256, 2016
DOI:10.1021/acs.jpcb.6b01435
View abstract

Heath GR, Li M, Polignano IL, Richens JL, Catucci G, O'Shea P, Sadeghi SJ, Gilardi G, Butt JN, Jeuken LJC Layer-by-layer assembly of supported lipid bilayer poly-l-lysine multilayers Biomacromolecules 17 324-335, 2016
DOI:10.1021/acs.biomac.5b01434
View abstract

Radu V, Frielingsdorf S, Lenz O, Jeuken LJC Reactivation from the Ni-B state in [NiFe] hydrogenase of Ralstonia eutropha is controlled by reduction of the superoxidised proximal cluster Chemical Communications 52 2632-2635, 2016
DOI:10.1039/c5cc10382g
View abstract

Jeuken LJC Structure and modification of electrode materials for protein electrochemistry In Advances in Biochemical Engineering/Biotechnology , 2016
DOI:10.1007/10_2015_5011
View abstract

Khan S, Li M, Muench SP, Jeuken LJC, Beales PA Durable proteo-hybrid vesicles for the extended functional lifetime of membrane proteins in bionanotechnology Chemical Communications 52 11020-11023, 2016
DOI:10.1039/c6cc04207d

Ye S, Benz F, Wheeler MC, Oram J, Baumberg JJ, Cespedes O, Christenson HK, Coletta PL, Jeuken LJC, Markham AF, Critchley K, Evans SD Data associated with’One-step Fabrication of Hollow-channel Gold Nanoflowers with Excellent Catalytic Performance and Large Single-particle SERS Activity’, 2016
DOI:10.5518/66

Li M, Jorgensen SK, McMillan DGG, Krzemniski L, Daskalakis NN, Partanen RH, Tutkus M, Tuma R, Stamou D, Hatzakis NS, Jeuken LJC Single Enzyme Experiments Reveal a Long-Lifetime Proton Leak State in a Heme-Copper Oxidase JOURNAL OF THE AMERICAN CHEMICAL SOCIETY 137 16055-16063, 2015
DOI:10.1021/jacs.5b08798

Hwang ET, Sheikh K, Orchard KL, Hojo D, Radu V, Lee CY, Ainsworth E, Lockwood C, Gross MA, Adschiri T, Reisner E, Butt JN, Jeuken LJC A decaheme cytochrome as a molecular electron conduit in dye-sensitized photoanodes Advanced Functional Materials 25 2308-2315, 2015
DOI:10.1002/adfm.201404541
View abstract

Radu V, Frielingsdorf S, Evans SD, Lenz O, Jeuken LJC Enhanced oxygen-tolerance of the full heterotrimeric membrane-bound [NiFe]-hydrogenase of Ralstonia eutropha. J Am Chem Soc 136 8512-8515, 2014
DOI:10.1021/ja503138p
View abstract

Mu Q, David CA, Galceran J, Rey-Castro C, Krzemiński L, Wallace R, Bamiduro F, Milne SJ, Hondow NS, Brydson R, Vizcay-Barrena G, Routledge MN, Jeuken LJC, Brown AP Systematic investigation of the physicochemical factors that contribute to the toxicity of ZnO nanoparticles. Chem Res Toxicol 27 558-567, 2014
DOI:10.1021/tx4004243
View abstract

Booth M, Peel R, Partanen R, Hondow N, Vasilca V, Jeuken LJC, Critchley K Amphipol-encapsulated CuInS<inf>2</inf>/ZnS quantum dots with excellent colloidal stability RSC Advances 3 20559-20566, 2013
DOI:10.1039/c3ra43846e
View abstract

McMillan DGG, Jeuken LJC, Marritt SJ, Kemp GL, Gordon-Brown P, Butt JN The impact of enzyme orientation and electrode topology on the catalytic activity of adsorbed redox enzymes Electrochimica Acta 110 79-85, 2013
DOI:10.1016/j.electacta.2013.01.153
View abstract

Gates AJ, Marritt SJ, Bradley JM, Shi L, McMillan DGG, Jeuken LJC, Richardson DJ, Butt JN Electrode assemblies composed of redox cascades from microbial respiratory electron transfer chains Biochemical Society Transactions 41 1249-1253, 2013
DOI:10.1042/BST20130147
View abstract

McMillan DG, Marritt SJ, Firer-Sherwood MA, Shi L, Richardson DJ, Evans SD, Elliott SJ, Butt JN, Jeuken LJ Protein-Protein Interaction Regulates the Direction of Catalysis and Electron Transfer in a Redox Enzyme Complex. Journal of the American Chemical Society 135 10550-10556, 2013
DOI:10.1021/ja405072z
View abstract

Marritt SJ, McMillan DGG, Shi L, Fredrickson JK, Zachara JM, Richardson DJ, Jeuken LJC, Butt JN The roles of CymA in support of the respiratory flexibility of Shewanella oneidensis MR-1 BIOCHEMICAL SOCIETY TRANSACTIONS 40 1217-1221, 2012
DOI:10.1042/BST20120150

Mu Q, Hondow NS, Krzeminski L, Brown AP, Jeuken LJC, Routledge MN Mechanism of cellular uptake of genotoxic silica nanoparticles PARTICLE AND FIBRE TOXICOLOGY 9 -, 2012
DOI:10.1186/1743-8977-9-29

Marritt SJ, Lowe TG, Bye J, McMillan DGG, Shi L, Fredrickson J, Zachara J, Richardson DJ, Cheesman MR, Jeuken LJC, Butt JN A functional description of CymA, an electron-transfer hub supporting anaerobic respiratory flexibility in Shewanella BIOCHEMICAL JOURNAL 444 465-474, 2012
DOI:10.1042/BJ20120197

Cheetham MR, Bramble JP, McMillan DGG, Bushby RJ, Olmsted PD, Jeuken LJC, Evans SD Manipulation and sorting of membrane proteins using patterned diffusion-aided ratchets with AC fields in supported lipid bilayers Soft Matter 8 5459-5465, 2012
DOI:10.1039/c2sm25473e
View abstract

McMillan DGG, Marritt SJ, Butt JN, Jeuken LJC Menaquinone-7 is specific cofactor in tetraheme quinol dehydrogenase CymA Journal of Biological Chemistry 287 14215-14225, 2012
DOI:10.1074/jbc.M112.348813
View abstract

Catucci G, Gilardi G, Jeuken L, Sadeghi SJ In vitro drug metabolism by C-terminally truncated human flavin-containing monooxygenase 3 Biochemical Pharmacology 83 551-558, 2012
DOI:10.1016/j.bcp.2011.11.029
View abstract

Daskalakis NN, Evans SD, Jeuken LJC Vesicle-modified electrodes to study proton-pumping by membrane proteins, 2011
DOI:10.1016/j.electacta.2011.06.073

Krzemiński L, Cronin S, Ndamba L, Canters GW, Aartsma TJ, Evans SD, Jeuken LJC Orientational control over nitrite reductase on modified gold electrode and its effects on the interfacial electron transfer. J Phys Chem B 115 12607-12614, 2011
DOI:10.1021/jp205852u
View abstract

Cheetham MR, Bramble JP, McMillan DGG, Krzeminski L, Han X, Johnson BRG, Bushby RJ, Olmsted PD, Jeuken LJC, Marritt SJ, Butt JN, Evans SD Concentrating Membrane Proteins Using Asymmetric Traps and AC Electric Fields J AM CHEM SOC 133 6521-6524, 2011
DOI:10.1021/ja2007615

Krzeminski L, Ndamba L, Canters GW, Aartsma TJ, Evans SD, Jeuken LJC Spectroelectrochemical Investigation of Intramolecular and Interfacial Electron-Transfer Rates Reveals Differences Between Nitrite Reductase at Rest and During Turnover J Am Chem Soc 133 15085-15093-15085-15093, 2011
DOI:10.1021/ja204891v
View abstract

Daskalakis NN, Muller A, Evans SD, Jeuken LJC Driving bioenergetic processes with electrodes SOFT MATTER 7 49-52, 2011
DOI:10.1039/c0sm01016b

Weiss SA, Bushby RJ, Evans SD, Jeuken LJC A study of cytochrome bo(3) in a tethered bilayer lipid membrane BBA - Bioenergetics 1797 1917-1923, 2010
DOI:10.1016/j.bbabio.2010.01.012
View abstract

Kendall JKR, Johnson BRG, Symonds PH, Imperato G, Bushby RJ, Gwyer JD, van Berkel C, Evans SD, Jeuken LJC Effect of the Structure of Cholesterol-Based Tethered Bilayer Lipid Membranes on Ionophore Activity CHEMPHYSCHEM 11 2191-2198, 2010
DOI:10.1002/cphc.200900917

Zhang LL, Jiang YH, Ding YL, Daskalakis N, Jeuken L, Povey M, O'Neill AJ, York DW Mechanistic investigation into antibacterial behaviour of suspensions of ZnO nanoparticles against E. coli J NANOPART RES 12 1625-1636, 2010
DOI:10.1007/s11051-009-9711-1

Weiss SA, Jeuken LJC Electrodes modified with lipid membranes to study quinone oxidoreductases Biochemical Society Transactions 37 707-712, 2009
DOI:10.1042/BST0370707
View abstract

Weiss SA, Bushby RJ, Evans SD, Henderson PJF, Jeuken LJC Characterization of cytochrome bo(3) activity in a native-like surface-tethered membrane Biochemical Journal 417 555-560, 2009
DOI:10.1042/BJ20081345
View abstract

Jeuken LJC Electrodes for integral membrane enzymes NAT PROD REP 26 1234-1240, 2009
DOI:10.1039/b903252e

Jeuken LJC, Weiss SA, Henderson PJF, Evans SD, Bushby RJ Impedance spectroscopy of bacterial membranes: coenzyme-Q diffusion in a finite diffusion layer. Anal Chem 80 9084-9090, 2008
DOI:10.1021/ac8015856
View abstract

Jeuken LJC, Weiss SA, Henderson PJF, Evans SD, Bushby RJ Impedance Spectroscopy of Bacterial Membranes: Coenzyme-Q Diffusion in a Finite Diffusion Layer Analytical Chemistry 80 9084-9090, 2008
DOI:10.1021/ac8015856
View abstract

Barfoot RJ, Sheikh KH, Johnson BRG, Colyer J, Miles RE, Jeuken LJC, Bushby RJ, Evans SD Minimal F-actin cytoskeletal system for planar supported phospholipid bilayers LANGMUIR 24 6827-6836, 2008
DOI:10.1021/la800085n

Jeuken LJC AFM study on the electric-field effects on supported bilayer lipid membranes Biophysical Journal 94 4711-4717, 2008
DOI:10.1529/biophysj.107.122887
View abstract

Dodd CE, Johnson BRG, Jeuken LJC, Bugg TDH, Bushby RJ, Evans SD Native E. coli inner membrane incorporation in solid-supported lipid bilayer membranes, 2008
DOI:10.1116/1.2896113

Dodd CE, Johnson BRG, Jeuken LJC, Evans SD Native E. coli inner membrane incorportaion in solid supported lipid bilayer membranes Biointerphases 3 FA59-FA67, 2008
DOI:10.1116/1.2896113
View abstract

Wijma HJ, Jeuken LJC, Verbeet MP, Armstrong FA, Canters GW Protein film voltammetry of copper-containing nitrite reductase reveals reversible inactivation J AM CHEM SOC 129 8557-8565, 2007
DOI:10.1021/ja071274q

Jeuken LJC, Daskalakis NN, Han XJ, Sheikh K, Erbe A, Bushby RJ, Evans SD Phase separation in mixed self-assembled monolayers and its effect on biomimetic membranes SENSOR ACTUAT B-CHEM 124 501-509, 2007
DOI:10.1016/j.snb.2007.01.014

Erbe A, Bushby RJ, Evans SD, Jeuken LJC Tethered bilayer lipid membranes studied by simultaneous attenuated total reflectance infrared spectroscopy and electrochemical impedance spectroscopy J PHYS CHEM B 111 3515-3524, 2007
DOI:10.1021/jp0676181

Jeuken LJC, Bushby RJ, Evans SD Proton transport into a tethered bilayer lipid membrane ELECTROCHEM COMMUN 9 610-614, 2007
DOI:10.1016/j.elecom.2006.10.045

Wijma HJ, Jeuken LJC, Verbeet MP, Armstrong FA, Canters GW A random-sequential mechanism for nitrite binding and active site reduction in copper-containing nitrite reductase J BIOL CHEM 281 16340-16346, 2006
DOI:10.1074/jbc.M601610200

Jeuken LJC, Connell SD, Henderson PJF, Gennis RB, Evans SD, Bushby RJ Redox enzymes in tethered membranes Journal of the American Chemical Society 128 1711-1716, 2006
DOI:10.1021/ja056972u
View abstract

Jeuken LJC, Connell SD, Nurnabi M, O'Reilly J, Henderson PJF, Evans SD, Bushby RJ Direct electrochemical interaction between a modified gold electrode and a bacterial membrane extract Langmuir 21 1481-1488, 2005
DOI:10.1021/la047732f
View abstract

Armstrong FA, Barlow NL, Burn PL, Hoke KR, Jeuken LJC, Shenton C, Webster GR Fast, long-range electron-transfer reactions of a 'blue' copper protein coupled non-covalently to an electrode through a stilbenyl thiolate monolayer CHEM COMMUN 316-317, 2004
DOI:10.1039/b312936e

Battistuzzi G, Bellei M, Borsari M, Canters GW, de Waal E, Jeuken LJC, Ranieri A, Sola M Control of metalloprotein reduction potential: Compensation phenomena in the reduction thermodynamics of blue copper proteins BIOCHEMISTRY-US 42 9214-9220, 2003
DOI:10.1021/bi034585w

Leger C, Elliott SJ, Hoke KR, Jeuken LJC, Jones AK, Armstrong FA Enzyme electrokinetics: Using protein film voltammetry to investigate redox enzymes and their mechanisms BIOCHEMISTRY-US 42 8653-8662, 2003
DOI:10.1021/bi034789c

Jeuken LJC Conformational reorganisation in interfacial protein electron transfer BBA-BIOENERGETICS 1604 67-76, 2003
DOI:10.1016/S0005-2728(03)00026-4

Jeuken LJC, Jones AK, Chapman SK, Cecchini G, Armstrong FA Electron-transfer mechanisms through biological redox chains in multicenter enzymes J AM CHEM SOC 124 5702-5713, 2002

van Gastel M, Nagano Y, Zondervan R, Canters GW, Jeuken LJC, Warmerdam GCM, de Waal EC, Groenen EJJ Hydrogen bonding in the blue-copper site. Resonance Raman study J PHYS CHEM B 106 4018-4021, 2002
DOI:10.1021/jp013839p

Jeuken LJC, Wisson LJ, Armstrong FA The kinetics of a weakly electron-coupled proton transfer in azurin INORG CHIM ACTA 331 216-223, 2002

Jeuken LJC, McEvoy JP, Armstrong FA Insights into gated electron-transfer kinetics at the electrode-protein interface: A square wave voltammetry study of the blue copper protein azurin J PHYS CHEM B 106 2304-2313, 2002
DOI:10.1021/jp0134291

Jeuken LJC, Camba R, Armstrong FA, Canters GW The pH-dependent redox inactivation of amicyanin from Paracoccus versutus as studied by rapid protein-film voltammetry J BIOL INORG CHEM 7 94-100, 2002
DOI:10.1007/s007750100269

Jeuken LJC, Armstrong FA Electrochemical origin of hysteresis in the electron-transfer reactions of adsorbed proteins: Contrasting behavior of the "blue" copper protein, azurin, adsorbed on pyrolytic graphite and modified gold electrodes J PHYS CHEM B 105 5271-5282, 2001
DOI:10.1021/jp004283t

van Amsterdam IMC, Ubbink M, Jeuken LJC, Verbeet MP, Einsle O, Messerschmidt A, Canters GW Effects of dimerization on protein electron transfer Chemistry a European journal 7 2398-2406, 2001
DOI:10.1002/1521-3765(20010601)7:11<2398::AID-CHEM23980>3.0.CO;2-L
View abstract

Remenyi R, Jeuken LJC, Comba P, Canters GW An amicyanin C-terminal loop mutant where the active-site histidine donor cannot be protonated J BIOL INORG CHEM 6 23-26, 2001

Jeuken LJC, van Vliet P, Verbeet MP, Camba R, McEvoy JP, Armstrong FA, Canters GW Role of the surface-exposed and copper-coordinating histidine in blue copper proteins: The electron-transfer and redox-coupled ligand binding properties of His117Gly azurin J AM CHEM SOC 122 12186-12194, 2000
DOI:10.1021/ja0006144

Jeuken LJC, Ubbink M, Bitter JH, van Vliet P, Meyer-Klaucke W, Canters GW The structural role of the copper-coordinating and surface-exposed histidine residue in the blue copper protein azurin Journal of Molecular Biology 299 737-755, 2000
View abstract

Farver O, Jeuken LJC, Canters GW, Pecht I Role of ligand substitution on long-range electron transfer in azurins European Journal of Biochemistry 267 3123-3129, 2000
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Buning C, Canters GW, Comba P, Dennison C, Jeuken LJC, Melter M, Sanders-Loehr J Loop-directed mutagenesis of the blue copper protein amicyanin from Paracoccus versutus and its effect on the structure and the activity of the type-1 copper site Journal of the American Chemical Society 122 204-211, 2000
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Armstrong FA, Camba R, Heering HA, Hirst J, Jeuken LJC, Jones AK, Leger C, McEvoy JP Fast voltammetric studies of the kinetics and energetics of coupled electron-transfer reactions in proteins Faraday Discussions 116 191-203, 2000
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Canters GW, Kolczak U, Armstrong FA, Jeuken LJC, Camba R, Sola M The effect of pH and ligand exchange on the redox properties of blue copper proteins Faraday Discussions 116 205-220, 2000
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Kalverda AP, Ubbink M, Gilardi G, Wijmenga SS, Crawford A, Jeuken LJC, Canters GW Backbone dynamics of azurin in solution: Slow conformational change associated with deprotonation of histidine 35 Biochemistry 38 12690-12697, 1999
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van Gastel M, Coremans JWA, Mol J, Jeuken LJC, Canters GW, Groenen EJJ The binding of imidazole in an azurin-like blue-copper site Journal of Biological Inorganic Chemistry 4 257-265, 1999
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van Gastel M, Coremans JWA, Jeuken LJC, Canters GW, Groenen EJJ Electron spin-echo envelope modulation spectrum of azurin at X-band Journal of Physical Chemistry A 102 4462-4470, 1998
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Albers WM, Lekkala JO, Jeuken L, Canters GW, Turner APF Design of novel molecular wires for realizing long-distance electron transfer Bioelectrochemistry and Bioenergetics 42 25-33, 1997
DOI:10.1016/S0302-4598(96)05150-1
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