Faculty of Biological Sciences

Dr Brian Jackson

BSc, PhD East Anglia
Research Facility Manager for Protein Production
School of Molecular and Cellular Biology

Background: BSc(Hons) 2000-2004; PhD 2004-2008, University of East Anglia. Postdoctoral Research Fellow 2008-2012; Wellcome Trust ISSF Junior Independent Research Fellow 2012-2014; Research Facility Manager for Bioimaging and Flow Cytometry 2015-2016; Research Facility Manager for Protein Production 2016-present, Faculty of Biological Sciences, University of Leeds.

Contact:  Astbury 9.108f, +44(0) 113 34 37369 (Lab: +44(0) 113 34 31253), email address for  

You can read more about Dr Jackson's interests here:
https://uk.linkedin.com/in/brian-jackson-a0405aa7
https://scholar.google.co.uk/citations?hl=en&pli=1&user=V6jZkwoAAAAJ
https://www.researchgate.net/profile/Brian_Jackson8

Research Interests

Protein Production Facility Manager

I trained as a Molecular Microbiologist, gaining my PhD in 2008 from the University of East Anglia. I gained substantial experience in recombinant DNA and protein technology throughout my PhD and postdoctoral positions, including the expression of archaeal, bacterial, mammalian and viral proteins. I moved from my own independent reserarch to Facility Management in 2015 when I took over management of the Bioimaging and Flow Cytometry Facilities at the University of Leeds. Now, with major investments in structural biology at Leeds, I have been appointed Facility Manager of the new Protein Production Facility (PPF).

 

Current Projects

We anticipate construction work to begin on the PPF in July 2016, with completion of the facility by Autumn 2016.

The Leeds PPF will offer the following services:

  • Vector design and cloning strategies
  • Protein expression optimisation
  • Protein purification from bacterial, insect or mammalian cells

Whilst the PPF is still in the design/construction stage, I am happy to discuss potential projects and can help with design, advice and small-scale lab work in the short term. Please get in touch for all your protein needs.

 

Faculty Research and Innovation

Modules taught

BIOL2211 - Human Diseases
BIOL5372M - Advanced Biomolecular Technologies
SPSC2203 - Exercise Biochemistry

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Dr Brian Jackson  (Research Facility Manager for Protein Production )

Protein Production Facility Manager 

Mr David Sharples  (Fermenter Technician)

Mrs Laura Wilkinson Hewitt  (Research Technician)


Research Facility Manager for Protein Production

Charles CJ, Rout SP, Patel KA, Akbar S, Laws AP, Jackson BR, Boxall SA, Humphreys PN Floc formation reduces the pH stress experienced by microorganisms living in alkaline environments Applied and Environmental Microbiology 83 -, 2017
DOI:10.1128/AEM.02985-16
View abstract

Schumann S, Jackson BR, Yule I, Whitehead SK, Revill C, Foster R, Whitehouse A Targeting the ATP-dependent formation of herpesvirus ribonucleoprotein particle assembly as an antiviral approach Nature Microbiology 2 -, 2016
DOI:10.1038/nmicrobiol.2016.201
View abstract

Wood JJ, Boyne JR, Paulus C, Jackson BR, Nevels MM, Whitehouse A, Hughes DJ ARID3B: A novel regulator of the Kaposi's sarcoma-associated herpesvirus lytic cycle Journal of Virology 90 9543-9555, 2016
DOI:10.1128/JVI.03262-15
View abstract

Hughes DJ, Wood JJ, Jackson BR, Baquero-Pérez B, Whitehouse A NEDDylation Is Essential for Kaposi’s Sarcoma-Associated Herpesvirus Latency and Lytic Reactivation and Represents a Novel Anti-KSHV Target PLoS Pathogens 11 -, 2015
DOI:10.1371/journal.ppat.1004771
View abstract

Owen CB, Hughes DJ, Baquero-Perez B, Berndt A, Schumann S, Jackson BR, Whitehouse A Utilising proteomic approaches to understand oncogenic human herpesviruses Molecular and Clinical Oncology 2 891-903, 2014
DOI:10.3892/mco.2014.341
View abstract

Jackson BR, Noerenberg M, Whitehouse A A novel mechanism inducing genome instability in Kaposi's sarcoma-associated herpesvirus infected cells. PLoS Pathog 10 e1004098-, 2014
DOI:10.1371/journal.ppat.1004098
View abstract

Griffiths D, Abdul-Sada H, Knight L, Jackson BR, Richards K, Prescott E, Peach H, Blair GE, Macdonald A, Whitehouse A Merkel cell polyomavirus small T antigen targets the NEMO adaptor protein to disrupt inflammatory signaling Journal of Virology 87 13853-13867, 2013
DOI:10.1128/JVI.02159-13
View abstract

Cobb AM, Jackson BR, Kim E, Bond PL, Bowater RP Sequence-specific and DNA structure-dependent interactions of Escherichia coli MutS and human p53 with DNA. Anal Biochem 442 51-61, 2013
DOI:10.1016/j.ab.2013.07.033
View abstract

Schumann S, Jackson BR, Baquero-Perez B, Whitehouse A Kaposi's sarcoma-associated herpesvirus ORF57 protein: exploiting all stages of viral mRNA processing Viruses 5 1901-1923, 2013
DOI:10.3390/v5081901
View abstract

Jackson BR, Noerenberg M, Whitehouse A The Kaposi's sarcoma-associated herpesvirus ORF57 protein and its multiple roles in mRNA biogenesis FRONTIERS IN MICROBIOLOGY 3 -, 2012
DOI:10.3389/fmicb.2012.00059

Taylor A, Jackson BR, Noerenberg M, Hughes DJ, Boyne JR, Verow M, Harris M, Whitehouse A Mutation of a C-terminal motif affects Kaposi's sarcoma-associated herpesvirus ORF57 RNA binding, nuclear trafficking, and multimerization. J Virol 85 7881-7891, 2011
DOI:10.1128/JVI.00138-11
View abstract

Jackson BR, Boyne JR, Noerenberg M, Taylor A, Hautbergue GM, Walsh MJ, Wheat R, Blackbourn DJ, Wilson SA, Whitehouse A An interaction between KSHV ORF57 and UIF provides mRNA-adaptor redundancy in herpesvirus intronless mRNA export. PLoS Pathog 7 e1002138-, 2011
DOI:10.1371/journal.ppat.1002138
View abstract

Tunnicliffe RB, Hautbergue GM, Kalra P, Jackson BR, Whitehouse A, Wilson SA, Golovanov AP Structural Basis for the Recognition of Cellular mRNA Export Factor REF by Herpes Viral Proteins HSV-1 ICP27 and HVS ORF57 PLOS PATHOG 7 -, 2011
DOI:10.1371/journal.ppat.1001244

Boyne JR, Jackson BR, Whitehouse A ORF57: Master regulator of KSHV mRNA biogenesis. Cell Cycle 9 2702-2703, 2010
DOI:10.4161/cc.9.14.12627

Boyne JR, Jackson BR, Taylor A, Macnab SA, Whitehouse A Kaposi's sarcoma-associated herpesvirus ORF57 protein interacts with PYM to enhance translation of viral intronless mRNAs. EMBO J 29 1851-1864, 2010
DOI:10.1038/emboj.2010.77
View abstract

Jackson BR, Noble C, Lavesa-Curto M, Bond PL, Bowater RP Characterization of an ATP-dependent DNA ligase from the acidophilic archaeon "Ferroplasma acidarmanus" Fer1. Extremophiles 11 315-327, 2007
DOI:10.1007/s00792-006-0041-2
View abstract