Faculty of Biological Sciences

Prof Elwyn Isaac

BSc, PhD 1978, Wales.
Professor of Comparative Biochemistry
School of Biology

Background: Senior Research Fellow in the Department of Biochemistry, University of Liverpool, Liverpool, UK, 1977-1983. Visiting Scientist in the Department of Entomolgy, Texas A&M University, College Station, Texas, USA, 1991 and at the Department d'Etudes et d'Ingéniérie des Protéins, Commissariat à l'Energie Atomique, CE-Saclay, Gif-Sur-Yvette, France, 1998-1999. Visiting Lecturer in the Department of Biological Sciences, University of Lancaster, Lancaster, UK, 2000-2003.

Contact:  Miall 8.15, +44(0) 113 34 32903, email address for  

Disrupting the expression of a C. elegans peptidase gene results in failure to replace old cuticle with new during a larval moult.

Research Interests

Comparative biochemistry and functional genomics of neuropeptides and peptidases

Our research exploits the amenable genetics and the sequenced genomes of the nematode, C. elegans, and the fly, D. melanogaster, to understand biochemical mechanisms of behaviour, development and reproduction.? We are particularly interested in the role of neuropeptides and peptidases in controlling the behaviour and sex-life of insects and in understanding the various physiological roles of members of large metallopeptidase and nucleoside transporter gene families in nematodes. We are also interested in the development of novel strategies to control parasitic nematodes and insect pests by identifying target proteins for inhibitors and vaccines.

 

Faculty Research and Innovation



Studentship information

Postgraduate studentship areas:

  • Structure and function of peptide hormones in Drosophila

See also:

Modules managed

BLGY2253 - Animals as Pests
BLGY3021 - Research Project
BLGY3340 - Biology Research Projects
BLGY3395 - Advanced Research Skills and Experience
BLGY3396 - Research Literature Review
BLGY3398 - Research Project and manuscript
BLGY3399 - Research Literature Review and Poster
BLGY5380M - Extended Research Project

Modules taught

BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5392M - Bioscience MSc Research Project
BLGY1125 - Biology Practicals and Data Analysis
BLGY2100/2301 - Level 2 Tutorials
BLGY2253 - Animals as Pests
BLGY3021 - Research Project
BLGY3340 - Biology Research Projects
BLGY3340/3399 - BLGY projects
BLGY3398 - Research Project and manuscript

Centre membership: Neuroscience Research at Leeds (NeuR@L)

Group Leader Prof Elwyn Isaac  (Professor of Comparative Biochemistry)

Comparative biochemistry and functional genomics of neuropeptides and peptidases 

Postgraduates

Zatul-'Iffah Abu Hasan (Primary supervisor) 90% FTE
Calum Ferguson (Primary supervisor) 50% FTE
Petra Pribylova (Primary supervisor) 50% FTE
Matthew Dooley (Co-supervisor) 20% FTE

Masuyer G, Schwager SLU, Sturrock ED, Isaac RE, Acharya KR Molecular recognition and regulation of human angiotensin-I converting enzyme (ACE) activity by natural inhibitory peptides SCIENTIFIC REPORTS 2 -, 2012
DOI:10.1038/srep00717

Ferguson CTJ, O'Neill TL, Audsley N, Isaac RE The sexually dimorphic behaviour of adult Drosophila suzukii: Elevated female locomotor activity and loss of siesta is a postmating response Journal of Experimental Biology 218 3855-3861, 2015
DOI:10.1242/jeb.125468
View abstract

Kim Y-J, Lee K-M, Elwyn Isaac R Battle of the sexes over paternity BMB Reports 48 241-242, 2015
DOI:10.5483/BMBRep.2015.48.5.067
View abstract

Iyer S, La-Borde PJ, Payne KAP, Parsons MR, Turner AJ, Isaac RE, Acharya KR Crystal structure of X-prolyl aminopeptidase from Caenorhabditis elegans: A cytosolic enzyme with a di-nuclear active site. FEBS Open Bio 5 292-302, 2015
DOI:10.1016/j.fob.2015.03.013
View abstract

Lee KM, Daubnerová I, Isaac RE, Zhang C, Choi S, Chung J, Kim YJ A neuronal pathway that controls sperm ejection and storage in female Drosophila. Curr Biol 25 790-797, 2015
DOI:10.1016/j.cub.2015.01.050
View abstract

Isaac RE, Kim YJ, Audsley N The degradome and the evolution of Drosophila sex peptide as a ligand for the MIP receptor. Peptides 53 258-264, 2014
DOI:10.1016/j.peptides.2013.12.016
View abstract

Masuyer G, Akif M, Czarny B, Beau F, Schwager SLU, Sturrock ED, Isaac RE, Dive V, Acharya KR Crystal structures of highly specific phosphinic tripeptide enantiomers in complex with the angiotensin-I converting enzyme FEBS JOURNAL 281 943-956, 2014
DOI:10.1111/febs.12660

Audsley N, Down RE, Isaac RE Genomic and peptidomic analyses of the neuropeptides from the emerging pest, Drosophila suzukii. Peptides -, 2013
DOI:10.1016/j.peptides.2014.08.006
View abstract

Karsai G, Pollak E, Wacker M, Voemel M, Selcho M, Berta G, Nachman RJ, Isaac RE, Molnar L, Wegener C Diverse in- and output polarities and high complexity of local synaptic and non-synaptic signaling within a chemically defined class of peptidergic Drosophila neurons FRONTIERS IN NEURAL CIRCUITS 7 -, 2013
DOI:10.3389/fncir.2013.00127

Isaac RE, Shirras AD Peptidyl-Dipeptidase A (Invertebrate) In Handbook of Proteolytic Enzymes (Third Edition) , 2013
DOI:10.1016/B978-0-12-382219-2.00099-5

Isaac RE, Shirras AD Nep2 Peptidase (Insect) In Handbook of Proteolytic Enzymes (Third Edition) , 2013
DOI:10.1016/B978-0-12-382219-2.00134-4

Akif M, Masuyer G, Bingham RJ, Sturrock ED, Isaac RE, Acharya KR Structural basis of peptide recognition by the angiotensin-1 converting enzyme homologue AnCE from Drosophila melanogaster FEBS JOURNAL 279 4525-4534, 2012
DOI:10.1111/febs.12038

Naccarati C, Audsley N, Keen JN, Kim JH, Howell GJ, Kim YJ, Isaac RE The host-seeking inhibitory peptide, Aea-HP-1, is made in the male accessory gland and transferred to the female during copulation. Peptides 34 150-157, 2012
DOI:10.1016/j.peptides.2011.10.027
View abstract

Akif M, Masuyer G, Schwager SL, Bhuyan BJ, Mugesh G, Isaac RE, Sturrock ED, Acharya KR Structural characterization of angiotensin I-converting enzyme in complex with a selenium analogue of captopril. FEBS J 278 3644-3650, 2011
DOI:10.1111/j.1742-4658.2011.08276.x
View abstract

Reiher W, Shirras C, Kahnt J, Baumeister S, Isaac RE, Wegener C Peptidomics and Peptide Hormone Processing in the Drosophila Midgut J PROTEOME RES 10 1881-1892, 2011
DOI:10.1021/pr101116g

Nachman RJ, Mahdian K, Nassel DR, Isaac RE, Pryor N, Smagghe G Biostable multi-Aib analogs of tachykinin-related peptides demonstrate potent oral aphicidal activity in the pea aphid Acyrthosiphon pisum (Hemiptera: Aphidae) PEPTIDES 32 587-594, 2011
DOI:10.1016/j.peptides.2010.09.013

Rao W, Isaac RE, Keen JN An analysis of the Caenorhabditis elegans lipid raft proteome using geLC-MS/MS J PROTEOMICS 74 242-253, 2011
DOI:10.1016/j.jprot.2010.11.001

Carhan A, Tang K, Shirras CA, Shirras AD, Isaac RE Loss of Angiotensin-converting enzyme-related (ACER) peptidase disrupts night-time sleep in adult Drosophila melanogaster J EXP BIOL 214 680-686, 2011
DOI:10.1242/jeb.049353

Janta M, Vomel M, Reiher W, Isaac RE, Roeder T, Wegener C Testing of an optical and mass spectrometric approach towards neuropeptide detection in the Drosophila hemolymph J NEUROGENET 24 54-55, 2010

Fortin SM, Marshall SL, Jaeger EC, Greene PE, Brady LK, Isaac RE, Schrandt JC, Brooks DR, Lyczak R The PAM-1 aminopeptidase regulates centrosome positioning to ensure anterior-posterior axis specification in one-cell C. elegans embryos DEV BIOL 344 992-1000, 2010
DOI:10.1016/j.ydbio.2010.06.016

Akif M, Ntai I, Sturrock ED, Isaac RE, Bachmann BO, Acharya KR Crystal structure of a phosphonotripeptide K-26 in complex with angiotensin converting enzyme homologue (AnCE) from Drosophila melanogaster BIOCHEM BIOPH RES CO 398 532-536, 2010
DOI:10.1016/j.bbrc.2010.06.113

Akif M, Georgiadis D, Mahajan A, Dive V, Sturrock ED, Isaac RE, Acharya KR High-Resolution Crystal Structures of Drosophila melanogaster Angiotensin-Converting Enzyme in Complex with Novel Inhibitors and Antihypertensive Drugs J MOL BIOL 400 502-517, 2010
DOI:10.1016/j.jmb.2010.05.024

Kim YJ, Bartalska K, Audsley N, Yamanaka N, Yapici N, Lee JY, Kim YC, Markovic M, Isaac E, Tanaka Y, Dickson BJ MIPs are ancestral ligands for the sex peptide receptor P NATL ACAD SCI USA 107 6520-6525, 2010
DOI:10.1073/pnas.0914764107

O'Toole JF, Liu YJ, Davis EE, Westlake CJ, Attanasio M, Otto EA, Seelow D, Nurnberg G, Becker C, Nuutinen M, Karppa M, Ignatius J, Uusimaa J, Pakanen S, Jaakkola E, van den Heuvel LP, Fehrenbach H, Wiggins R, Goyal M, Zhou WB, Wolf MTF, Wise E, Helou J, Allen SJ, Murga-Zamalloa CA, Ashraf S, Chaki M, Heeringa S, Chernin G, Hoskins BE, Chaib H, Gleeson J, Kusakabe T, Suzuki T, Isaac RE, Quarmby LM, Tennant B, Fujioka H, Tuominen H, Hassinen I, Lohi H, van Houten JL, Rotig A, Sayer JA, Rolinski B, Freisinger P, Madhavan SM, Herzer M, Madignier F, Prokisch H, Nurnberg P, Jackson P, Khanna H, Katsanis N, Hildebrandt F Individuals with mutations in XPNPEP3, which encodes a mitochondrial protein, develop a nephronophthisis-like nephropathy J CLIN INVEST 120 791-802, 2010
DOI:10.1172/JCI40076

Isaac RE, Li CX, Leedale AE, Shirras AD Drosophila male sex peptide inhibits siesta sleep and promotes locomotor activity in the post-mated female P R SOC B 277 65-70, 2010
DOI:10.1098/rspb.2009.1236

Isaac RE, Bland ND, Shirras AD Neuropeptidases and the metabolic inactivation of insect neuropeptides GEN COMP ENDOCR 162 8-17, 2009
DOI:10.1016/j.ygcen.2008.12.011

Taneja-Bageshwar S, Strey A, Isaac RE, Coast GM, Zubrzak P, Pietrantonio PV, Nachman RJ Biostable agonists that match or exceed activity of native insect kinins on recombinant arthropod GPCRs GEN COMP ENDOCR 162 122-128, 2009
DOI:10.1016/j.ygcen.2008.10.013

Bland ND, Robinson P, Thomas JE, Shirras AD, Turner AJ, Isaac RE Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2 PEPTIDES 30 571-574, 2009
DOI:10.1016/j.peptides.2008.10.020

Nachman RJ, Ben Aziz O, Davidovitch M, Zubrzak P, Isaac RE, Strey A, Reyes-Rangel G, Juaristi E, Williams HJ, Altstein M Biostable beta-amino acid PK/PBAN analogs: Agonist and antagonist properties PEPTIDES 30 608-615, 2009
DOI:10.1016/j.peptides.2008.11.007

Bland ND, Pinney JW, Thomas JE, Turner AJ, Isaac RE Bioinformatic analysis of the neprilysin (M13) family of peptidases reveals complex evolutionary and functional relationships BMC EVOL BIOL 8 -, 2008
DOI:10.1186/1471-2148-8-16

Isaac RE, Johnson EC, Audsley N, Shirras AD Metabolic inactivation of the circadian transmitter, pigment dispersing factor (PDF), by neprilysin-like peptidases in Drosophila J EXP BIOL 210 4465-4470, 2007
DOI:10.1242/jeb.012088

Rylett CM, Walker MJ, Howell GJ, Shirras AD, Isaac RE Male accessory glands of Drosophila melanogaster make a secreted angiotensin I-converting enzyme (ANCE), suggesting a role for the peptide-processing enzyme in seminal fluid J EXP BIOL 210 3601-3606, 2007
DOI:10.1242/jeb.009035

Craig H, Isaac RE, Brooks DR Unravelling the moulting degradome: new opportunities for chemotherapy? TRENDS PARASITOL 23 248-253, 2007
DOI:10.1016/j.pt.2007.04.003

Zubrzak P, Williams H, Coast GM, Isaac RE, Reyes-Rangel G, Juaristi E, Zabrocki J, Nachman RJ β-amino acid analogs of an insect neuropeptide feature potent bioactivity and resistance to peptidase hydrolysis Biopolymers - Peptide Science Section 88 76-82, 2007
DOI:10.1002/bip.20638
View abstract

Bland ND, Thomas JE, Audsley N, Shirras AD, Turner AJ, Isaac RE Expression of NEP2, a soluble neprilysin-like endopeptidase, during embryogenesis in Drosophila melanogaster PEPTIDES 28 127-135, 2007
View abstract

Zubrzak P, Williams H, Coast GM, Isaac RE, Reyes-Rangel G, Juaristi E, Zabrocki J, Nachman RJ beta-amino acid analogs of an insect neuropeptide feature potent bioactivity and resistance to peptidase hydrolysis BIOPOLYMERS 88 76-82, 2007
DOI:10.1002/bip.20638

Isaac RE, Lamango NS, Ekbote U, Taylor CA, Hurst D, Weaver RJ, Carhan A, Burnham S, Shirras AD Angiotensin-converting enzyme as a target for the development of novel insect growth regulators PEPTIDES 28 153-162, 2007
DOI:10.1016/j.peptides.2006.08.029

Isaac RE, Johnson EC, Shirras AD Metabolic inactivation of PDF in adult Drosophila by neprilysin J NEUROGENET 20 135-135, 2006

Walker MJ, Rylett CM, Keen JN, Audsley N, Sajid M, Shirras AD, Isaac RE Proteomic identification of Drosophila melanogaster male accessory gland proteins, including a pro-cathepsin and a soluble gamma-glutamyl transpeptidase PROTEOME SCI 4 -, 2006
DOI:10.1186/1477-5956-4-9

Bingham RJ, Dive V, Phillips SEV, Shirras AD, Isaac RE Structural diversity of angiotensin-converting enzyme - Insights from structure-activity comparisons of two Drosophila enzymes FEBS J 273 362-373, 2006
DOI:10.1111/j.1742-4658.2005.05069.x

Burnham S, Smith JA, Lee AJ, Isaac RE, Shirras AD The angiotensin-converting enzyme (ACE) gene family of Anopheles gambiae BMC Genomics 6 pp.172-, 2005
DOI:10.1186/1471-2164-6-172
View abstract

Visser F, Baldwin SA, Isaac RE, Young JD, Cass CE Identification and mutational analysis of amino acid residues involved in dipyridamole interactions with human and Caenorhabditis elegans equilibrative nucleoside transporters J BIOL CHEM 280 11025-11034, 2005
DOI:10.1074/jbc.M410348200

Thomas JE, Rylett CM, Carhan A, Bland ND, Bingham RJ, Shirras AD, Turner AJ, Isaac RE Drosophila melanogaster NEP2 is a new soluble member of the neprilysin family of endopeptidases with implications for reproduction and renal function. Biochemical Journal 386 357-366, 2005
DOI:10.1042/BJ20041753
View abstract

Isaac RE, Taylor CA, Hamasaka Y, Nässel DR, Shirras AD Proctolin in the post-genomic era: new insights and challenges. Invert Neurosci 5 51-64, 2004
DOI:10.1007/s10158-004-0029-5
View abstract

Appleford PJ, Griffiths M, Yao SY, Ng AM, Chomey EG, Isaac RE, Coates D, Hope IA, Cass CE, Young JD, Baldwin SA Functional redundancy of two nucleoside transporters of the ENT family (CeENT1, CeENT2) required for development of Caenorhabditis elegans. Mol Membr Biol 21 247-259, 2004
DOI:10.1080/09687680410001712550
View abstract

Isaac RE Peptidyl-dipeptidase A (invertebrate) In Handbook of Proteolytic Enzymes: Second Edition , 2004
DOI:10.1016/B978-0-12-079611-3.50091-4

Rodgers-Gray TP, Smith JE, Ashcroft AE, Isaac RE, Dunn AM Mechanisms of parasite-induced sex reversal in Gammarus duebeni INT J PARASITOL 34 747-753, 2004
DOI:10.1016/j.ijpara.2004.01.005

Taylor CAM, Winther AME, Siviter RJ, Shirras AD, Isaac RE, Nassel DR Identification of a proctolin preprohormone gene (Proct) of Drosophila melanogaster: Expression and predicted prohormone processing J NEUROBIOL 58 379-391, 2004
DOI:10.1002/neu.10301

Brooks DR, Appleford PJ, Murray L, Isaac RE An essential role in molting and morphogenesis of Caenorhabditis elegans for ACN-1, a novel member of the angiotensin-converting enzyme family that lacks a metallopeptidase active site. J Biol Chem 278 52340-52346, 2003
DOI:10.1074/jbc.M308858200
View abstract

Ekbote UV, Weaver RJ, Isaac RE Angiotensin I-converting enzyme (ACE) activity of the tomato moth, Lacanobia oleracea: changes in levels of activity during development and after copulation suggest roles during metamorphosis and reproduction INSECT BIOCHEM MOLEC 33 989-998, 2003
DOI:10.1016/S0965-1748(03)00105-X

Dani MP, Richards EH, Isaac RE, Edwards JP Antibacterial and proteolytic activity in venom from the endoparasitic wasp Pimpla hypochondriaca (Hymenoptera : Ichneumonidae) J INSECT PHYSIOL 49 945-954, 2003
DOI:10.1016/S0022-1910(03)00163-X

Winther AME, Siviter RJ, Isaac RE, Predel R, Nassel DR Neuronal expression of tachykinin-related peptides and gene transcript during postembryonic development of Drosophila J COMP NEUROL 464 180-196, 2003
DOI:10.1002/cne.10790

Ekbote U, Looker M, Isaac RE ACE inhibitors reduce fecundity in the mosquito, Anopheles stephensi Comparative Biochemistry&Physiology B134 593-598, 2003

Isaac RE, Nässel DR Identification and localization of a neprilysin-like activity that degrades tachykinin-related peptides in the brain of the cockroach, Leucophaea maderae, and locust, Locusta migratoria. J Comp Neurol 457 57-66, 2003
DOI:10.1002/cne.10561
View abstract

Hurst D, Rylett CM, Isaac RE, Shirras AD The drosophila angiotensin-converting enzyme homologue Ance is required for spermiogenesis Developmental Biology 254 238-247, 2003
DOI:10.1016/S0012-1606(02)00082-9

Brooks DR, Hooper NM, Isaac RE The Caenorhabditis elegans orthologue of mammalian puromycin-sensitive aminopeptidase has roles in embryogenesis and reproduction Journal of Biological Chemistry 278 42795-42801, 2003
DOI:10.1074/jbc.M306216200
View abstract

Brooks DR, Isaac RE Functional genomics of parasitic worms: The dawn of a new era PARASITOL INT 51 319-325, 2002

Siviter RJ, Nachman RJ, Dani M, Keen JN, Shirras AD, Isaac RE Peptidyl dipeptidases (Ance and Acer) of Drosophila melanogaster: major differences in the substrate specificity of two homologs of human angiotensin I-converting enzyme Peptides 23 2025-2034, 2002
DOI:10.1016/S0196-9781(02)00190-0

Wilson CL, Shirras AD, Isaac RE Extracellular peptidases of imaginal discs of Drosophila melanogaster PEPTIDES 23 2007-2014, 2002

Siviter RJ, Taylor CAM, Cottam DM, Denton A, Dani MP, Milner MJ, Shirras AD, Isaac RE Ance, a Drosophila angiotensin-converting enzyme homologue, is expressed in imaginal cells during metamorphosis and is regulated by the steroid, 20-hydroxyecdysone BIOCHEM J 367 187-193, 2002
DOI:10.1042/BJ20020567

Lee AJ, Machell J, Van den Broek A, Nisbet AJ, Miller HRP, Isaac RE, Huntley JF Identification of an antigen from the sheep scab mite, Psoroptes ovis, homologous with house dust mite group I allergens Parasite Immunology 24 413-422, 2002
DOI:10.1046/j.1365-3024.2002.00480.x

Lee AJ, Huntley J, Van den Broek A, Coates D, Isaac RE Expression and characterisation of a Psoroptes ovis glutathione S-transferase VET PARASITOL 105 49-63, 2002

Nachman RJ, Strey A, Isaac RE, Pryor N, Lopez JD, Deng JG, Coast GM Enhanced in vivo activity of peptidase-resistant analogs of the insect kinin neuropeptide family Peptides 23 735-745, 2002
DOI:10.1016/S0196-9781(01)00654-4
View abstract

Isaac RE, Parkin ET, Keen JN, Nassel DR, Siviter RJ, Shirras AD Inactivation of a tachykinin-related peptide: identification of four neuropeptide-degrading enzymes in neuronal membranes of insects from four different orders PEPTIDES 23 725-733, 2002

Laurent V, Brooks DR, Coates D, Isaac RE Functional expression and characterization of the cytoplasmic aminopeptidase P of Caenorhabditis elegans. Eur J Biochem 268 5430-5438, 2001
View abstract

Isaac RE, Siviter RJ, Stancombe PR, Coates D, Shirras A Conserved roles for peptidases in the processing of invertebrate neuropeptides. Biochemical Society Transactions 28, part 4 -, 2000

Tilley SB, Weaver RJ, Isaac RE Allatostatin-like and AKH/HrTH-like peptides in the aphid Megoura viciae. Gen Comp Endocrinol 117 355-365, 2000
DOI:10.1006/gcen.1999.7415
View abstract

Coates D, Isaac RE, Cotton J, Siviter RJ, Williams TA, Shirras A, Corvol P, Dive V Functional conservation of the active sites of human and Drosophila angiotensin I-converting enzyme ChemBioChem 39 8963-8969, 2000

Siviter RJ, Coast GM, Winther AME, Nachman RJ, Taylor CAM, Shirras A, Coates D, Isaac RE, Nassel DR Expression and functional characterisation of a Drosophila neuropeptide precursor with homology to mammalian preprotachykinin Journal of Biological Chemistry 275 23273-23280, 2000

Coates D, Siviter R, Isaac RE Exploring the Caenorhabditis elegans and Drosophila melanogaster genomes to understand neuropeptide and peptidase function. Biochem Soc Trans 28 464-469, 2000
View abstract

Isaac RE, Michaud A, Keen JN, Williams TA, Coates D, Wetsel WC, Corvol P Hydrolysis by somatic angiotensin-I converting enzyme of basic dipeptides from a cholecystokinin/gastrin and a LH-RH peptide extended at the C-terminus with gly-Arg/Lys-arg, but not from diarginyl insulin. Eur J Biochem 262 569-574, 1999
View abstract

Isaac RE, Ekbote U, Coates D, Shirras AD Insect angiotensin-converting enzyme. A processing enzyme with broad substrate specificity and a role in reproduction. Ann N Y Acad Sci 897 342-347, 1999
View abstract

Ekbote U, Coates D, Isaac RE A mosquito (Anopheles stephensi) angiotensin I-converting enzyme (ACE) is induced by a blood meal and accumulates in the developing ovary FEBS Letters 455 219-252, 1999

Lee AJ, Isaac RE, Coates D The construction of a cDNA expression library for the sheep scab mite Psoroptes ovis Veterinary Parasitology 83 241-252, 1999

Isaac RE, Veelaert D, Schoofs L, Macours N, Vandingenen A, deLoof A, Salzet M, Huybrechts R Immunocytochemical distribution of angiotensin-I converting enzyme in the central nervous system of insects and speculations about its possible function. Eur. J. Entomology 96 323-332, 1999

Loeb MJ, De Loof A, Schoofs L, Isaac E Angiotensin II and angiotensin-converting enzyme as candidate compounds modulating the effects of testis ecdysiotropin in testes of the gypsy moth, Lymantria dispar GEN COMP ENDOCR 112 232-239, 1998

Houard X, Williams TA, Michaud A, Dani P, Isaac RE, Shirras AD, Coates D, Corvol P The Drosophila melanogaster-related angiotensin-I-converting enzymes Acer and Ance - Distinct enzymic characteristics and alternative expression during pupal development EUROPEAN JOURNAL OF BIOCHEMISTRY 257 599-606, 1998
DOI:10.1046/j.1432-1327.1998.2570599.x

Pennock JL, Behnke JM, Bickle QD, Devaney E, Grencis RK, Isaac RE, Joshua GWP, Selkirk ME, Zhang YB, Meyer DJ Rapid purification and characterization of L-dopachrome-methyl ester tautomerase (macrophage-migration-inhibitory factor) from Trichinella spiralis, Trichuris muris and Brugia pahangi BIOCHEM J 335 495-498, 1998

Nachman RJ, Muren JE, Isaac RE, Lundquist CT, Karlsson A, Nassel DR An aminoisobutyric acid-containing analogue of the cockroach tachykinin-related peptide, LemTRP-1, with potent bioactivity and resistance to an insect angiotensin-converting enzyme REGUL PEPTIDES 74 61-66, 1998

Schoofs L, Veelaert D, De Loof A, Huybrechts R, Isaac E Immunocytochemical distribution of angiotensin I-converting enzyme-like immunoreactivity in the brain and testis of insects BRAIN RES 785 215-227, 1998

Pennock JD, Behnke JM, Bickle QM, Devaney E, Grencis RK, Isaac RE, Selkirk ME, Zhang Y, Meyer DJ Rapid purification and characterisation of macrophage-migration inhibitory factor (D-dopachrome tautomerase) from Trichinella spiralis, Trichuris muris and Brugia pahangi Biochemical Journal 257 599-606, 1998

Isaac RE, Schoofs L, Williams TA, Veelaert D, Sajid M, Corvol P, Coates D A novel peptide processing activity of insect peptidyl-dipeptidase A (angiotensin I-converting enzyme): the hydrolysis of lysyl-arginine and arginyl-arginine from the C-terminus of an insect prohormone peptide Biochemical Journal 330 61-65, 1998

Isaac RE, Williams TA, Sajid M, Corvol P, Coates D Cleavage of arginyl-arginine and lysyl-arginine from the C- terminus of pro-hormone peptides by human germinal angiotensin I-converting enzyme (ACE) and the C-domain of human somatic ACE Biochemical Journal 328 587-591, 1997
View abstract

Sajid M, Isaac RE, Harrow ID Purification and properties of a membrane aminopeptidase from Ascaris suum muscle that degrades neuropeptides AF1 and AF2. Mol Biochem Parasitol 89 225-234, 1997
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MacGregor D, Williams D, Perry RN, Coates D, Isaac RE Characterisation of a C. elegans neurotransmitter transporter gene. Biochem Soc Trans 25 552S-, 1997

Coates D, Briggs DA, MacGregor D, Lynch AS, Kolakowski LF, Hope IA, Isaac RE Characterisation of ZK643-3: a putative 7TM neuropeptide receptor. Biochem Soc Trans 25 440S-, 1997

Isaac RE, Lamango NS, Nachman RJ, Strey A, Hayes TK Angiotensin-converting enzyme and the metabolism of regulatory peptides in insects. Ann N Y Acad Sci 814 339-341, 1997

Lamango NS, Nachman RJ, Hayes TK, Strey A, Isaac RE Hydrolysis of insect neuropeptides by an angiotensin-converting enzyme from the housefly, Musca domestica. Peptides 18 47-52, 1997
View abstract

Isaac RE Purification and properties of a membrane aminopeptidase Molecular and Biochemical Parasitology 89 225-342, 1997

James C, Macgregor D, Lynch AS, Coates D, Isaac RE The Expression of a C. Elegans Neurotransmitter Transporter Gene (T25B6.2), 1997

Isaac RE Characterisation of a neurotransmitter transporter gene from Caenorhabditis elegans Biochemical Society Transactions 25 552-552, 1997

Macgregor D, Lynch AS, Coates D, Isaac RE Characterisation of a Neurotransmitter Gene from Caenorhabditis Elegans, 1997

Isaac RE The expression of a C.elegans neurotransmitter transporter gene(T25B6.2) Biochemical Society Transactions 25 553-553, 1997

Isaac RE Peptidyl dipeptidase A in invertebrates Handbook of Proteolytic Enzymes 1076-1077, 1997

Nachman RJ, Isaac RE, Coast GM, Holman GM Aib-containing analogues of the insect kinin neuropeptide family demonstrate resistance to an insect angiotensin-converting enzyme and potent diuretic activity Peptides 18 53-57, 1997

Isaac RE, Schoofs L, Williams TA, Veelaert D, Sajid M, Coates D Towards a role for angiotensin-converting enzyme in insects New York Academy of Sciences. Annals 839 288-292, 1997

Nachman RJ, Isaac RE, Coast GM, Roberts VA, Lange A, Orchard I, Holman GM, Favrel P Active conformation and mimetic analog development for the pyrokinin/PBAN/myosuppressin insect neuropeptide families Natural Pest Control Agents -, 1997

Nachman RJ, Isaac RE, Coast GM, Holman GM Potent, Ance, endopeptidase resistant, Aibcontaining analogues ofn the diuretic insect kinin neuropeptides… New York Academy of Sciences. Annals 814 331-334, 1997

Meyer DJ, Muimo R, Thomas M, Coates D, Isaac RE Purification and characterization of prostaglandin-H E-isomerase, a sigma-class glutathione S-transferase, from Ascaridia galli BIOCHEM J 313 223-227, 1996

Sajid M, Keating C, Holden-Dye L, Harrow ID, Isaac RE Metabolism of AF1 (KNEFIRF-NH2) in the nematode, Ascaris suum, by aminopeptidase endopeptidase and enzymes. Molecular and Biochemical Parasitology 75 159-168, 1996

Isaac RE Purification and charcetrization of prostaglandin… Biochemical Journal 313 223-227, 1996

Isaac RE The endopeptidase activity and the conservation by Cl of angiotensin-converting enzyme is evolutionarily conserved: purification and properties of an angiotensin-converting enzyme from the house fly, Musca domestica. Biochemical Journal 314 639-646, 1996

Isaac RE, Macgregor D, Coates D Metabolism and inactivation of neurotransmitters in nematodes Parasitology 113 157-173, 1996

Cornell MJ, Williams TA, Lamango NS, Coates D, Corvol P, Soubrier F, Hoheisel J, Lehrach H, Isaac RE Cloning and expression of an evolutionary conserved single-domain angiotensin converting enzyme from Drosophila melanogaster. J Biol Chem 270 13613-13619, 1995
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Sajid M, Isaac RE Identification and properties of a neuropeptide-degrading endopeptidase (neprilysin) of Ascarus suum muscle. Parasitology 111 599-608, 1995

Isaac RE, Lamango NS Peptidyl dipeptidase activity in the haemolymph of insects. Biochem Soc Trans 22 292S-, 1994

Davenport TR, Eaves LA, Hayes TK, Lee DL, Isaac RE The detection of AKH/HrTH-like peptides in Ascaridia galli and Ascaris suum using an insect hyperglycaemic bioassay. Parasitology 108 ( Pt 4) 479-485, 1994
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Sajid M, Isaac RE Metabolism of AF1 (KNEFiRF-NH2) in the nematode Ascaris suum Biochemical Society Transactions 22 -, 1994

Isaac RE Identification and properties of a peptidyl dipeptidase in the housefly, Musca domestica, that resembles mammalian angiotensin-converting enzyme Biochemical Journal 299 651-657, 1994

Muimo R, Isaac RE Properties of an arylalkylamine N-acetyltransferase from the nematode, Ascaridia galli. Comp Biochem Physiol B 106 969-976, 1993
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Lamango NS, Isaac RE Metabolism of insect neuropeptides: properties of a membrane-bound endopeptidase from heads of Musca domestica. Insect Biochem Mol Biol 23 801-808, 1993
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Cornell MJ, Coates D, Isaac RE Characterisation of putative Drosophila angiotensin converting enzyme cDNA clones. Biochem Soc Trans 21 ( Pt 3) 243S-, 1993

Lamango N, Isaac RE Identification of an ACE-like peptidyl dipeptidase activity in the housefly, Musca domestica. Biochem Soc Trans 21 ( Pt 3) 245S-, 1993

Muimo R, Isaac RE Metabolism of 5-HT in the nematode Caenorhabditis elegans: identification of a 5-HT N-acetyltransferase that is developmentally regulated. Biochem Soc Trans 21 ( Pt 3) 295S-, 1993

KEMPTON LRC, ISAAC RE, PILLMOOR JB, WILLIS RJ OCTOPAMINE N-ACETYLTRANSFERASE ACTIVITY FROM THE CATTLE TICK, BOOPHILUS-MICROPLUS INSECT BIOCHEM MOLEC 22 777-783, 1992

Isaac RE, Eaves L, Muimo R, Lamango N N-acetylation of biogenic amines in Ascaridia galli. Parasitology 102 Pt 3 445-450, 1991

Davenport TR, Isaac RE, Lee DL The presence of peptides related to the adipokinetic hormone/red pigment-concentrating hormone family in the nematode, Panagrellus redivivus. Gen Comp Endocrinol 81 419-425, 1991
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Isaac RE, Muimo R, MacGregor AN N-acetylation of serotonin, octopamine and dopamine by adult Brugia pahangi. Mol Biochem Parasitol 43 193-198, 1990
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ATKINSON HJ, ISAAC RE, HARRIS PD, SHARPE CM FMRFAMIDE-LIKE IMMUNOREACTIVITY WITHIN THE NERVOUS-SYSTEM OF THE NEMATODES PANAGRELLUS-REDIVIUS, CAENORHABDITIS-ELEGANS AND HETERODERA-GLYCINES J ZOOL 216 663-671, 1988

Isaac RE Neuropeptide-degrading endopeptidase activity of locust (Schistocerca gregaria) synaptic membranes. Biochem J 255 843-847, 1988
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Davenport TR, Lee DL, Isaac RE Immunocytochemical demonstration of a neuropeptide in Ascaris suum (Nematoda) using an antiserum to FMRFamide. Parasitology 97 ( Pt 1) 81-88, 1988
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Slinger AJ, Isaac RE Synthesis of apolar ecdysone esters by ovaries of the cockroach Periplaneta americana. Gen Comp Endocrinol 70 74-82, 1988
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SLINGER AJ, ISAAC RE ACYL-COA-ECDYSONE ACYLTRANSFERASE ACTIVITY FROM THE OVARY OF P-AMERICANA INSECT BIOCHEM 18 779-784, 1988

ISAAC RE THE METABOLISM OF NEUROPEPTIDES BY MEMBRANE PEPTIDASES FROM THE LOCUST, SCHISTOCERCA-GREGARIA PESTIC SCI 24 259-260, 1988

SLINGER AJ, ISAAC RE ECDYSTEROID TITERS DURING EMBRYOGENESIS OF THE COCKROACH, PERIPLANETA-AMERICANA J INSECT PHYSIOL 34 1119-1125, 1988

Isaac RE Proctolin degradation by membrane peptidases from nervous tissues of the desert locust (Schistocerca gregaria). Biochem J 245 365-370, 1987
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ATKINSON HJ, FOWLER M, ISAAC RE PARTIAL-PURIFICATION OF HATCHING ACTIVITY FOR GLOBODERA-ROSTOCHIENSIS FROM POTATO ROOT DIFFUSATE ANN APPL BIOL 110 115-125, 1987

SLINGER AJ, DINAN LN, ISAAC RE ISOLATION OF APOLAR ECDYSTEROID CONJUGATES FROM NEWLY-LAID OOTHECAE OF PERIPLANETA-AMERICANA INSECT BIOCHEM 16 115-119, 1986

ISAAC RE THE USE OF SHORT 3-MU-HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COLUMNS FOR THE RAPID ANALYSIS OF INSECT HORMONES BIOCHEM SOC T 13 1238-1239, 1985

Nässel DR, Birse RT, Isaac R, Muren E, WInther AME Biology of tachykinin-related peptides in arthropods In Arthropods 2001, Chemical, Physiological and Environmental Aspects ,

Isaac R, Brooks DR, Hope IA Neurobiology and development of nematodes In Neurobiology From molecular basis to Disease ,

Isaac R, Audsley N Insect Peptide Hormones In Amino acids, Peptides and Proteins in Organic Chemistry ,