Background: B.Sc., Lic. Universidade Estadual de Campinas (Brazil), M.Sc. University of Illinois at Urbana-Champaign (USA), Ph.D. University of Illinois at Urbana-Champaign (USA), Postdoctoral Fellow University of California Los Angeles (USA)
Contact: Garstang G5.55a, +44(0) 113 34 34291,
Structural and Functional Plasticity in the CNS : activity dependent plasticity in the spinal cord
The mechanisms related to the neural control of movement in mammalian systems are largely unanswered, simply because the intricacies of interneuronal communication and computations necessary to produce coordinated, voluntary movements are of such complexity that we have only begun to understand them. Relative to the overwhelming complexity of the supraspinal control of movement, the spinal neural circuits provide a simpler model to investigate motor control issues. This does not, however, imply that spinal control of movement in mammalian species is a simple model. My research has focused on the changes within the spinal cord that occur after a complete spinal cord transection and on the activity-dependent plasticity in the spinal neural circuits associated with locomotor training after the spinal cord injury. We have used behavioural, immunohistochemical, electron microscopic and electrophysiologic methods to investigate those issues. The combined results strongly suggest that the biochemical, structural, and electrophysiological properties of motoneurons change dramatically within the spinal cord isolated from the brain, which are altered by locomotor training. Understanding these processes in detail will provide critical insight into the mechanisms involved in the control and learning of movements within spinal cord neural circuits.
A,B. Detailed kinematics analysis of movement and physiological measurements such as EMG (C) are combined to study the neuronal events that lead to plasticity in the CNS. D, shows selective spinal motoneurons (green) active during locomotion (red).
Another interest in my lab is the neural control of cardiovascular function related to movement production and exercise. During muscular activity, two basic mechanisms control cardiovascular adjustments: a central command and a reflex arising from the contracting muscles, which is dependent on medullary centers. I have investigated issues related to both of those mechanisms. We have implicated higher cortical centers (insular cortex) in the central command circuitry. In addition, I have demonstrated that specific locomotor and cardiovascular control areas in the brain change with exercise training. The posterior hypothalamus nucleus, the mesencephalic locomotor region, periaqueductal grey, nucleus of the tractus solitarius and the rostral ventrolateral medulla showed diminished, possibly more efficient, activation profiles (cfos) in exercised than in non-exercised rats in response to a single bout of controlled exercise.
After a spinal cord injury, the remaining unaffected spinal tissue changes to a great extent. A successful neural regenerative strategy will have to overcome not only all the obstacles that the injury site itself presents (glial scaring, physical gap, etc.) but also a new environment that has formed below the level of the lesion. We hypothesize that the beneficial effects of locomotor training will potentiate the effects of regenerative strategies. We have combined locomotor training with different potential neural regenerative strategies, with both positive and surprising results. Given the nature of spinal cord injuries, we firmly believe that only a combination strategy will be effective in regenerating and forming functional synaptic reconnections after an injury.
We have developed a technique to epidurally stimulate the spinal cord, which produces alternating and coordinated steps in completely spinalized adult rats. This technique allows us to study the control of locomotion in an in vivo adult mammalian preparation, which was not possible previously. We have used this technique to investigate reflex control mechanisms in the intact spinal cord and also after a complete spinal transection.
SPSC5382 - Extended Research Project
BMSC3126/43/44/45/46 - Integrative Biomedical Sciences/Advanced Topics I
SPSC2240 - Human Motor Development
Member of Graduate School Committee (Progression Tutor (SBMS))
Centre membership: Neuroscience Research at Leeds (NeuR@L)
Dr Samit Chakrabarty (Lecturer in Neuroscience)
Neurophysiology of Motor control
Dr Varinder Lall (Research Fellow)