Faculty of Biological Sciences

Dr Nikita Gamper

MS, PhD 1998, St Petersburg
Associate Professor in Neuroscience
School of Biomedical Sciences

Background: 1995-1998 PhD Physiology, Sechenov Institute of Evolutionary Physiology and Biochemistry, St Petersburg, Russia. 1999-2001 Postdoctral Fellow, Tuebingen University, Germany. 2001-2005 Postdoctoral Fellow, University of Texas Health Science Center at San Antonio, USA

Contact:  Garstang 6.31d | +44(0) 113 34 37923 | email address for  

Separation of electrical charges on the plasma membrane of the cell achieved by the complex balance of activities of different ion channels, transporters and pumps is a fundamental principle of life form organization. Dysfunctions of ion channels or their regulation causes a number of human diseases ranging from epilepsy to sudden cardiac death. We study function and regulation of ion channels and, in larger perspective, regulation of cellular excitability. Particular focus of the group is the regulation of excitability of peripheral pain-sensing (nociceptive) neurones in relation to pain signalling. We investigate the regulation of ion channels that control or influence excitability and synaptic transmission of nociceptors (e.g. KCNQ or M channels, Ca2+-activated Cl- channels, sensory TRP channels and voltage-gated Ca2+ channels). Our group is also interested in the modulation of different neuronal ion channels by G protein coupled receptors (GPSRs) and in GPCR signalling networks of sensory neurones. One of the latest projects is aimed at the investigation of the transcriptional regulation of neuronal K+ channels in chronic pain states.

We use a wide range of the cutting-edge methods and techniques which allow us to follow our research questions from the level of individual molecules and up to the whole-organism level. Thus we use biochemical and molecular biological approaches to study individual proteins and genes; we use electrophysiology (patch-clamp and voltage clamp) and bioimaging methods (including live confocal and TIRF microscopy) to study individual cells and, finally, we use various behavioural approaches to probe neuronal signalling in vivo.

Fundibg: Wellcome Trust, MRC, BBSRC and the Royal Society

 
 

Faculty Research and Innovation


Studentship information

Postgraduate studentship areas:

  • Postgraduate studentship: strong candidates willing to do a PHD in the area of neuronal ion channel regulation are encouraged to apply

See also:

Admin roles

FBS UGTSEC
UG Examinations Officer

Modules managed

BMSC2222 - Mechanisms of Brain Function

Modules taught

BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2118 - Neurobiology
BMSC2120 - Scientific Skills
BMSC2222 - Mechanisms of Brain Function
BMSC2223/BMSC3302 - Neuropharmacology/Medical Pharmacology
BMSC2231 - Topics in Neuroscience
BMSC3140 - Advanced Scientific Skills
BMSC3143/44/45/46 - ATUs - BMS specific
BMSC3301 - Research Proj: Biomed Sciences

Committees

Member of Undergraduate School Taught Student Education Committee

Centre membership: Neuroscience Research at Leeds (NeuR@L)

Mr Haixia Gao  (Research Fellow)

Dr Sylvain Gigout  (Research Fellow)

Miss Hannah Marie Kirton  (Research Fellow)

Mrs Katarzyna Marszalek  (Research Technician)


Postgraduates

Alexandra Gherghina  
Xin Jin  
Shihab Shah  

Gamper N; Ooi L Redox and Nitric Oxide-Mediated Regulation of Sensory Neuron Ion Channel Function. Antioxid Redox Signal -, 2014
DOI:10.1089/ars.2014.5884
View abstract

Peers C; Boyle JP; Scragg JL; Dallas ML; Al-Owais MM; Hettiarachichi NT; Elies J; Johnson E; Gamper N; Steele DS Diverse mechanisms underlying the regulation of ion channels by carbon monoxide. Br J Pharmacol -, 2014
DOI:10.1111/bph.12760
View abstract

Liu Y; Zhang H; Huang D; Qi J; Xu J; Gao H; Du X; Gamper N; Zhang H Characterization of the effects of Cl(-) channel modulators on TMEM16A and bestrophin-1 Ca(2+) activated Cl (-) channels. Pflugers Arch -, 2014
DOI:10.1007/s00424-014-1572-5
View abstract

Rose KE; Gigout S; Gamper N M-Current Recording from Acute DRG Slices. Methods Mol Biol 998 311-320, 2013
DOI:10.1007/978-1-62703-351-0_25
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Kirton HM; Pettinger L; Gamper N Transient overexpression of genes in neurons using nucleofection. Methods Mol Biol 998 55-64, 2013
DOI:10.1007/978-1-62703-351-0_4
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Ooi L; Gigout S; Pettinger L; Gamper N Triple cysteine module within M-type K+ channels mediates reciprocal channel modulation by nitric oxide and reactive oxygen species. J Neurosci 33 6041-6046, 2013
DOI:10.1523/JNEUROSCI.4275-12.2013
View abstract

Boycott HE; Dallas ML; Elies J; Pettinger L; Boyle JP; Scragg JL; Gamper N; Peers C Carbon monoxide inhibition of Cav3.2 T-type Ca2+ channels reveals tonic modulation by thioredoxin. FASEB J 27 3395-3407, 2013
DOI:10.1096/fj.13-227249
View abstract

Pettinger L; Gigout S; Linley JE; Gamper N Bradykinin controls pool size of sensory neurons expressing functionalδ-opioid receptors. J Neurosci 33 10762-10771, 2013
DOI:10.1523/JNEUROSCI.0123-13.2013
View abstract

Jin X; Shah S; Liu Y; Zhang H; Lees M; Fu Z; Lippiat JD; Beech DJ; Sivaprasadarao A; Baldwin SA; Zhang H; Gamper N Activation of the Cl- channel ANO1 by localized calcium signals in nociceptive sensory neurons requires coupling with the IP3 receptor. Sci Signal 6 ra73-, 2013
DOI:10.1126/scisignal.2004184
View abstract

Du X; Gamper N Potassium Channels in Peripheral Pain Pathways: Expression, Function and Therapeutic Potential CURRENT NEUROPHARMACOLOGY 11 621-640, 2013

Linley JE; Pettinger L; Huang D; Gamper N M channel enhancers and physiological M channel block JOURNAL OF PHYSIOLOGY-LONDON 590 793-807, 2012
DOI:10.1113/jphysiol.2011.223404

Hettiarachchi NT; Boyle JP; Bauer CC; Dallas ML; Pearson HA; Hara S; Gamper N; Peers C Peroxynitrite mediates disruption of Ca2+ homeostasis by carbon monoxide via Ca2+ ATPase degradation. Antioxid Redox Signal 17 744-755, 2012
DOI:10.1089/ars.2011.4398
View abstract

Gamper N; Rohacs T Phosphoinositide sensitivity of ion channels, a functional perspective. Subcell Biochem 59 289-333, 2012
DOI:10.1007/978-94-007-3015-1_10
View abstract

Linley JE; Ooi L; Pettinger L; Kirton H; Boyle JP; Peers C; Gamper N Reactive oxygen species are second messengers of neurokinin signaling in peripheral sensory neurons. Proc Natl Acad Sci U S A 109 E1578-E1586, 2012
DOI:10.1073/pnas.1201544109
View abstract

Jiang LH; Gamper N; Beech DJ Properties and Therapeutic Potential of Transient Receptor Potential Channels with Putative Roles in Adversity: Focus on TRPC5, TRPM2 and TRPA1 CURR DRUG TARGETS 12 724-736, 2011

Rose K; Ooi L; Dalle C; Robertson B; Wood IC; Gamper N Transcriptional repression of the M channel subunit Kv7.2 in chronic nerve injury PAIN 152 742-754, 2011
DOI:10.1016/j.pain.2010.12.028

Jopling HM; Howell GJ; Gamper N; Ponnambalam S The VEGFR2 receptor tyrosine kinase undergoes constitutive endosome-to-plasma membrane recycling BIOCHEM BIOPH RES CO 410 170-176, 2011
DOI:10.1016/j.bbrc.2011.04.093

Linley JE; Rose K; Ooi L; Gamper N Understanding inflammatory pain: ion channels contributing to acute and chronic nociception PFLUG ARCH EUR J PHY 459 657-669, 2010
DOI:10.1007/s00424-010-0784-6

Liu BY; Linley JE; Du XN; Zhang X; Ooi L; Zhang HL; Gamper N The acute nociceptive signals induced by bradykinin in rat sensory neurons are mediated by inhibition of M-type K+ channels and activation of Ca2+-activated Cl- channels J CLIN INVEST 120 1240-1252, 2010
DOI:10.1172/JCI41084

Gamper N Osmosensitivity through the PIP2 availability: just add water J PHYSIOL-LONDON 588 3631-3632, 2010
DOI:10.1113/jphysiol.2010.197871

Mucha M; Ooi L; Linley JE; Mordaka P; Dalle C; Robertson B; Gamper N; Wood IC Transcriptional Control of KCNQ Channel Genes and the Regulation of Neuronal Excitability J NEUROSCI 30 13235-13245, 2010
DOI:10.1523/JNEUROSCI.1981-10.2010

Ooi L; Linley J; Gamper N Substance P triggers two different signaling pathways with opposing actions on M current mediated by intracellular Ca2+rises and oxidative modification FASEB JOURNAL 24 -, 2010

Shapiro MS; Gamper N Regulation of Neuronal Ion Channels by G-Protein-Coupled Receptors in Sympathetic Neurons In Structure, Function and Modulation of Neuronal Voltage-Gated Ion Channels. , 2009

Milligan CJ; Li J; Sukumar P; Majeed Y; Dallas ML; English A; Emery P; Porter KE; Smith AM; McFadzean I; Beccano-Kelly D; Bahnasi Y; Cheong A; Naylor J; Zeng FN; Liu X; Gamper N; Jiang LH; Pearson HA; Peers C; Robertson B; Beech DJ Robotic multiwell planar patch-clamp for native and primary mammalian cells NAT PROTOC 4 244-255, 2009
DOI:10.1038/nprot.2008.230

Linley JE; Rose K; Patil M; Robertson B; Akopian AN; Gamper N Inhibition of M Current in Sensory Neurons by Exogenous Proteases: A Signaling Pathway Mediating Inflammatory Nociception J NEUROSCI 28 11240-11249, 2008
DOI:10.1523/JNEUROSCI.2297-08.2008

Gamper N; Shapiro MS Target-specific PIP2 signalling: how might it work? J PHYSIOL-LONDON 582 967-975, 2007
View abstract

Gamper N; Shapiro MS Regulation of ion transport proteins by membrane phosphoinositides NAT REV NEUROSCI 8 921-934, 2007
DOI:10.1038/nrn2257

Zaika O; Lara LS; Gamper N; Hilgemann D; Jaffe DB; Shapiro MS Angiotensin II regulates neuronal excitability via phosphatidylinositol 4,5-bisphosphate-dependent modulation of Kv7 (M-type) K+ channels J PHYSIOL-LONDON 575 49-67, 2006
DOI:10.1113/jphysiol.2006.114074

Pochynyuk O; Medina J; Gamper N; Genth H; Stockand JD; Staruschenko A Rapid translocation and insertion of the epithelial Na+ channel in response to RhoA signaling J BIOL CHEM 281 26520-26527, 2006
DOI:10.1074/jbc.M603716200

Patwardhan AM; Jeske NA; Price TJ; Gamper N; Akopian AN; Hargreaves KM The cannabinoid WIN 55,212-2 inhibits transient receptor potential vanilloid 1 (TRPV1) and evokes peripheral antihyperalgesia via calcineurin P NATL ACAD SCI USA 103 11393-11398, 2006
DOI:10.1073/pnas.0603861103

Jeske NA; Patwardhan AM; Gamper N; Price TJ; Akopian AN; Hargreaves KM Cannabinoid WIN 55,212-2 regulates TRPV1 phosphorylation in sensory neurons J BIOL CHEM 281 32879-32890, 2006
DOI:10.1074/jbc.M603220200

Gamper N; Shapiro MS Exogenous expression of proteins in neurons using the biolistic particle delivery system. Methods Mol Biol 337 27-38, 2006
DOI:10.1385/1-59745-095-2:27
View abstract

Gamper N; Zaika O; Li Y; Martin P; Hernandez CC; Perez MR; Wang AY; Jaffe DB; Shapiro MS Oxidative modification of M-type K+ channels as a mechanism of cytoprotective neuronal silencing EMBO J 25 4996-5004, 2006
DOI:10.1038/sj.emboj.7601374

Gamper N; Reznikov V; Yamada Y; Yang J; Shapiro MS Phosphatidylinositol 4,5-bisphosphate signals underlie receptor-specific G(q/11)-mediated modulation of N-type Ca2+ channels. (vol 24, pg 10980, 2004) J NEUROSCI 25 -, 2005

Gamper N; Li Y; Shapiro MS Structural requirements for differential sensitivity of KCNQ K+ channels to modulation by Ca2+/calmodulin MOL BIOL CELL 16 3538-3551, 2005
DOI:10.1091/mbc.E04-09-0849

Delmas P; Coste B; Gamper N; Shapiro MS Phosphoinositide lipid second messengers: New paradigms for calcium channel modulation NEURON 47 179-182, 2005
DOI:10.1016/j.neuron.2005.07.001

Gamper N; Stockand JD; Shapiro MS The use of Chinese hamster ovary (CHO) cells in the study of ion channels. J Pharmacol Toxicol Methods 51 177-185, 2005
DOI:10.1016/j.vascn.2004.08.008
View abstract

Li Y; Gamper N; Hilgemann DW; Shapiro MS Regulation of Kv7 (KCNQ) K+ channel open probability by phosphatidylinositol 4,5-bisphosphate J NEUROSCI 25 9825-9835, 2005
DOI:10.1523/JNEUROSCI.2597-05.2005

Patwardhan AM; Berg KA; Akopain AN; Jeske NA; Gamper N; Clarke WP; Hargreaves KM Bradykinin-induced functional competence and trafficking of the delta-opioid receptor in trigeminal nociceptors J NEUROSCI 25 8825-8832, 2005
DOI:10.1523/JNEUROSCI.0160-05.2005

Li Y; Langlais P; Gamper N; Liu F; Shapiro MS Dual phosphorylations underlie modulation of unitary KCNQ K+ channels by Src tyrosine kinase J BIOL CHEM 279 45399-45407, 2004
DOI:10.1074/jbc.M408410200

Li Y; Gamper N; Shapiro MS Single-channel analysis of KCNQ K+ channels reveals the mechanism of augmentation by a cysteine-modifying reagent J NEUROSCI 24 5079-5090, 2004
DOI:10.1523/JNEUROSCI.0882-04.2004

Gamper N; Reznikov V; Yamada Y; Yang J; Shapiro MS Phosphatidylinositol [correction] 4,5-bisphosphate signals underlie receptor-specific Gq/11-mediated modulation of N-type Ca2+ channels. J Neurosci 24 10980-10992, 2004
DOI:10.1523/JNEUROSCI.3869-04.2004
View abstract

Tong Q; Gamper N; Medina JL; Shapiro MS; Stockand JD Direct activation of the epithelial Na(+) channel by phosphatidylinositol 3,4,5-trisphosphate and phosphatidylinositol 3,4-bisphosphate produced by phosphoinositide 3-OH kinase. J Biol Chem 279 22654-22663, 2004
DOI:10.1074/jbc.M401004200
View abstract

Gamper N; Shapiro MS Calmodulin mediates Ca2+-dependent modulation of M-type K+ channels. J Gen Physiol 122 17-31, 2003
DOI:10.1085/jgp.200208783
View abstract

Gamper N; Stockand JD; Shapiro MS Subunit-specific modulation of KCNQ potassium channels by Src tyrosine kinase. J Neurosci 23 84-95, 2003
View abstract

Braĭlovskaia IV; Gamper NL; Savina MV [External pathway of NADH oxidation in the liver of lamprey Lampetra fluviatilis]. Zh Evol Biokhim Fiziol 39 201-204, 2003

Bock J; Szabo I; Gamper N; Adams C; Gulbins E Ceramide inhibits the potassium channel Kv1.3 by the formation of membrane platforms BIOCHEM BIOPH RES CO 305 890-897, 2003
DOI:10.1016/S0006-291X(03)00763-0

Gamper N; Shapiro MS Calmodulin mediates Ca2+-dependent modulation of M-type K+ channels J GEN PHYSIOL 122 17-31, 2003
DOI:10.1085/gp.200208783

Huber S; Uhlemann A; Gamper N; Duranton C; Lang F; Kremsner P Oxidative permeabilization? Trends Parasitol 18 346-, 2002

Huber SM; Uhlemann AC; Gamper NL; Duranton C; Kremsner PG; Lang F Plasmodium falciparum activates endogenous Cl(-) channels of human erythrocytes by membrane oxidation. EMBO J 21 22-30, 2002
DOI:10.1093/emboj/21.1.22
View abstract

Gamper N; Fillon S; Huber SM; Feng Y; Kobayashi T; Cohen P; Lang F IGF-1 up-regulates K+ channels via PI3-kinase, PDK1 and SGK1. Pflugers Arch 443 625-634, 2002
DOI:10.1007/s00424-001-0741-5
View abstract

Tanneur V; Ilgaz D; Duranton C; Fillon S; Gamper N; Huber SM; Lang F Time-dependent regulation of capacitative Ca2+ entry by IGF-1 in human embryonic kidney cells. Pflugers Arch 445 74-79, 2002
DOI:10.1007/s00424-002-0859-0
View abstract

Gamper N; Fillon S; Feng Y; Friedrich B; Lang PA; Henke G; Huber SM; Kobayashi T; Cohen P; Lang F K+ channel activation by all three isoforms of serum- and glucocorticoid-dependent protein kinase SGK. Pflugers Arch 445 60-66, 2002
DOI:10.1007/s00424-002-0873-2
View abstract

Hendron E; Patel P; Hausenfluke M; Gamper N; Shapiro MS; Booth RE; Stockand JD Identification of cytoplasmic domains within the epithelial Na+ channel reactive at the plasma membrane J BIOL CHEM 277 34480-34488, 2002
DOI:10.1074/jbc.M204615200

Gamper N; Savina MV; Brailovskaja IV; Vereninov AA Respiration rates, ATP content and ionic regulation in hepatocytes of starving lamprey during the pre-spawning period of their life cycle Journal of Fish Biology 58 230-239, 2001
DOI:10.1111/j.1095-8649.2001.tb00510.x
View abstract

Fillon S; Warntges S; Matskevitch J; Moschen I; Setiawan I; Gamper N; Feng YX; Stegen C; Friedrich B; Waldegger S; Broer S; Wagner CA; Huber SM; Klingel K; Vereninov A; Lang F Serum- and glucocorticoid-dependent kinase, cell volume, and the regulation of epithelial transport COMP BIOCHEM PHYS A 130 367-376, 2001
View abstract

Huber SM; Gamper N; Lang F Chloride conductance and volume-regulatory nonselective cation conductance in human red blood cell ghosts PFLUG ARCH EUR J PHY 441 551-558, 2001

Gamper N; Huber SM; Badawi K; Lang F Cell volume-sensitive sodium channels unregulated by glucocorticoids in U937 macrophages PFLUG ARCH EUR J PHY 441 281-286, 2000

Lang F; Ritter M; Gamper N; Huber S; Fillon S; Tanneur V; Lepple-Wienhues A; Szabo I; Gulbins E Cell volume in the regulation of cell proliferation and apoptotic cell death CELL PHYSIOL BIOCHEM 10 417-428, 2000

Connell SDA; Vakurov A; Galluzzi M; Podesta A; Gamper N; Nelson A Direct characterisation of fluid lipid assemblies on mercury in electric fields ACS Nano -,