Faculty of Biological Sciences

Dr Juan Fontana

University Academic Fellowship in Correlative Light and Electron Microscopy
School of Molecular and Cellular Biology

Contact:  Astbury 8.109, +44(0) 113 34 34170, email address for  

You can read more about Dr Fontana's interests here:
http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=JF

Faculty Research and Innovation

Modules taught

BIOL3398 - Research Tools and Applications
BIOL3399 - Extended Research Project Preparation

Postgraduates

Lauren Brown (Primary supervisor) 50% FTE

Newcomb WW, Fontana J, Winkler DC, Cheng N, Heymann JB, Steven AC The Primary Enveloped Virion of Herpes Simplex Virus 1: Its Role in Nuclear Egress mBio 8 e00825-17-e00825-17, 2017
DOI:10.1128/mBio.00825-17
View abstract

Fontana J, Keller PW, Urano E, Ablan SD, Steven AC, Freed EO Identification of an HIV-1 mutation in spacer peptide 1 that stabilizes the immature CA-SP1 lattice Journal of Virology 90 972-978, 2016
DOI:10.1128/JVI.02204-15
View abstract

Aksyuk AA, Newcomb WW, Cheng N, Winkler DC, Fontana J, Bernard Heymann J, Steven AC Subassemblies and Asymmetry in Assembly of Herpes Simplex Virus Procapsid mBio 6 -, 2015
DOI:10.1128/mBio.01525-15
View abstract

Fontana J, Jurado KA, Cheng N, Ly NL, Fuchs JR, Gorelick RJ, Engelman AN, Steven AC Distribution and Redistribution of HIV-1 Nucleocapsid Protein in Immature, Mature, and Integrase-Inhibited Virions: a Role for Integrase in Maturation Journal of Virology 89 9765-9780, 2015
DOI:10.1128/JVI.01522-15
View abstract

Fontana J, Steven AC Influenza virus-mediated membrane fusion: Structural insights from electron microscopy Archives of Biochemistry and Biophysics 581 86-97, 2015
DOI:10.1016/j.abb.2015.04.011
View abstract

McHugh CA, Fontana J, Nemecek D, Cheng N, Aksyuk AA, Heymann JB, Winkler DC, Lam AS, Wall JS, Steven AC, Hoiczyk E A virus capsid-like nanocompartment that stores iron and protects bacteria from oxidative stress EMBO Journal 33 1896-1911, 2014
DOI:10.15252/embj.201488566
View abstract

Cairns TM, Fontana J, Huang Z-Y, Whitbeck JC, Atanasiu D, Rao S, Shelly SS, Lou H, Ponce de Leon M, Steven AC, Eisenberg RJ, Cohen GH Mechanism of Neutralization of Herpes Simplex Virus by Antibodies Directed at the Fusion Domain of Glycoprotein B Journal of Virology 88 2677-2689, 2014
DOI:10.1128/JVI.03200-13
View abstract

Cheng N, Wu W, Watts NR, Fontana J, Steven AC Turning a liability into an asset: Radiation damage as a cytochemical marker, 2014
DOI:10.1017/S1431927614007971

Fontana J, Steven AC At Low pH, influenza virus matrix protein M1 undergoes a conformational change prior to dissociating from the membrane Journal of Virology 87 5621-5628, 2013
DOI:10.1128/JVI.00276-13
View abstract

Verma S, Dimitrova M, Munjal A, Fontana J, Crevar CJ, Carter DM, Ross TM, Khurana S, Goldinga H Oligomeric recombinant H5 HA1 vaccine produced in bacteria protects ferrets from homologous and heterologous wild-type H5N1 influenza challenge and controls viral loads better than subunit h5n1 vaccine by eliciting high-affinity antibodies Journal of Virology 86 12283-12293, 2012
DOI:10.1128/JVI.01596-12
View abstract

Cardone G, Brecher M, Fontana J, Winkler DC, Butan C, White JM, Steven AC Visualization of the two-step fusion process of the retrovirus avian sarcoma/leukosis virus by cryo-electron tomography Journal of Virology 86 12129-12137, 2012
DOI:10.1128/JVI.01880-12
View abstract

Fontana J, Cardone G, Heymann JB, Winkler DC, Steven AC Structural changes in influenza virus at low ph characterized by cryo-electron tomography Journal of Virology 86 2919-2929, 2012
DOI:10.1128/JVI.06698-11
View abstract

Khurana S, Larkin C, Verma S, Joshi MB, Fontana J, Steven AC, King LR, Manischewitz J, McCormick W, Gupta RK, Golding H Recombinant HA1 produced in E. coli forms functional oligomers and generates strain-specific SRID potency antibodies for pandemic influenza vaccines Vaccine 29 5657-5665, 2011
DOI:10.1016/j.vaccine.2011.06.014
View abstract

Fontana J, López-Iglesias C, Tzeng WP, Frey TK, Fernández JJ, Risco C Three-dimensional structure of Rubella virus factories Virology 405 579-591, 2010
DOI:10.1016/j.virol.2010.06.043
View abstract

Diestra E, Fontana J, Guichard P, Marco S, Risco C Visualization of proteins in intact cells with a clonable tag for electron microscopy Journal of Structural Biology 165 157-168, 2009
DOI:10.1016/j.jsb.2008.11.009
View abstract

Fontana J, López-Montero N, Elliott RM, Fernández JJ, Risco C The unique architecture of Bunyamwera virus factories around the Golgi complex Cellular Microbiology 10 2012-2028, 2008
DOI:10.1111/j.1462-5822.2008.01184.x
View abstract

Fontana J, Tzeng WP, Calderita G, Fraile-Ramos A, Frey TK, Risco C Novel replication complex architecture in rubella replicon-transfected cells Cellular Microbiology 9 875-890, 2007
DOI:10.1111/j.1462-5822.2006.00837.x
View abstract

Novoa RR, Calderita G, Arranz R, Fontana J, Granzow H, Risco C Virus factories: Associations of cell organelles for viral replication and morphogenesis Biology of the Cell 97 147-172, 2005
DOI:10.1042/BC20040058
View abstract