Faculty of Biological Sciences

Prof John Colyer

BSc, CNAA; MPhil, London; PhD 1988, Southampton
Professor of Biotechnology
School of Biomedical Sciences

Background: BSc; CNAA; MPhil, London. PhD, Southampton. Post-doctoral work in the MRC Group in Signal Transduction, University of Calgary, Canada. British Heart Foundation Lecturer at University of Leeds, 1991-99. Senior Lecturer, 1999-current. Founder of Fluoroscience Ltd (biotech/drug discovery company), 1998

Contact:  Garstang 7.53b, +44(0) 113 34 33124, email address for  

You can read more about Prof Colyer's interests here:
www.bmb.leeds.ac.uk/staff/jc/homepage/homepage.htm

Research Interests

Natural & Therapeutic Control of Cardiac Function

We are interested in the control of cardiac muscle behaviour by physiological and pathological stresses. These stresses control cardiac function by post-translational modification of key proteins (phosphorylation, oxidation, etc.) in Excitation-Contraction Coupling, and we develop new tools to define the role of these site-specific modifications in physiological and pathological circumstances.

Our research has direct application to industry, and we collaborate with companies – most notably Badrilla, a spin-out company from this laboratory – to produce new experimental tools for the cardiovascular field, and to develop quantitative biomarker detection approaches for systems biology.

 

Current Projects

Oxidation of CaMKII in Health & Disease

Calmodulin-dependent protein kinase II (CaMKII) is a key regulator of physiological behaviour in the heart and elsewhere. Oxidation of specific residues in CaMKII (Met-281/282) occurs in pathological situations, and leads to long lasting enzyme activity which underpins the pathological phenotype. In collaboration with chemists and clinicians at Leeds, we are exploring the pattern of oxidation of CaMKII (site- and stereo-specific oxidation of individual residues), characterising new tools for the measurement of particular oxidation states and examining the association of oxidation with particular vascular diseases.

An MSc by Research position is available in this project (July 2013).

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Oxidation of CaMKII in Health & Disease
    Keywords: peptide chemistry, mass spectrometry, immunoassay. (Laboratory)
  • Protein phosphorylation in cardiac muscle contraction
    Keywords: Signal transduction, cardiac myocytes, immunotechniques (blotting/microscopy), microinjection (Laboratory)
  • Control of cellular differentiation: Cellular transplantation therapies
    Keywords: In title (Literature)

Postgraduate studentship areas:

  • Oxidation of CaMKII in Health & Disease

See also:

Modules managed

BIOL5265M - Investigational New Drug (IND) Project
BIOL5269M - Commercialising Biopharmaceutical Products
BMSC2120 - Scientific Skills

Modules taught

BIOL2210/BIOC2301 - Integrated Biochemistry/Biological Membranes
BIOL5162M - Biopharmaceutical Drug Discovery
BIOL5265M - Investigational New Drug (IND) Project
BIOL5269M - Commercialising Biopharmaceutical Products
BIOL5371M - Research Planning and Scientific Communication
BIOL5397M - Practical Skills for a Regulatory Environment
BMSC1103 - Basic Laboratory and Scientific Skills
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC2120 - Scientific Skills
BMSC3101 - Inherited Disorders
BMSC3301 - Research Project in Biomedical Sciences

Committees

Member of Masters Taught Student Education Committee

Centre membership: The Multidisciplinary Cardiovascular Research Centre (MCRC)

Group Leader Prof John Colyer  (Professor of Biotechnology)

Natural & Therapeutic Control of Cardiac Function  

Dr Moninder Bhogal  (Visiting Research Fellow)

Dr Kathleen Wright  (Senior Scientific Officer in Cardiovascular Science)

Providing laboratory research support for multiple PIs, training new staff and laboratory/office management 


Postgraduates

Thomas Sheard (Co-supervisor) 33% FTE

Bennett RJ, Simpson DM, Holman SW, Ryan S, Brownridge P, Eyers CE, Colyer J, Beynon RJ DOSCATs: Double standards for protein quantification Scientific Reports 7 -, 2017
DOI:10.1038/srep45570
View abstract

Vallée MR, Schombs MW, Balaban ZJ, Colyer J, Davis BG Ready display of antigenic peptides in a protein 'mimogen' Chemical Communications 52 3014-3017, 2016
DOI:10.1039/c5cc08892e
View abstract

Macdougall DA, Agarwal SR, Stopford EA, Chu H, Collins JA, Longster AL, Colyer J, Harvey RD, Calaghan S Caveolae compartmentaliseβ2-adrenoceptor signals by curtailing cAMP production and maintaining phosphatase activity in the sarcoplasmic reticulum of the adult ventricular myocyte. J Mol Cell Cardiol 52 388-400, 2012
DOI:10.1016/j.yjmcc.2011.06.014
View abstract

Carter S, Pitt SJ, Colyer J, Sitsapesan R Ca2+-Dependent Phosphorylation of RyR2 Can Uncouple Channel Gating from Direct Cytosolic Ca2+ Regulation J MEMBRANE BIOL 240 21-33, 2011
DOI:10.1007/s00232-011-9339-9

Chase A, Colyer J, Orchard CH Localised Ca channel phosphorylation modulates the distribution of L-type Ca current in cardiac myocytes J MOL CELL CARDIOL 49 121-131, 2010
DOI:10.1016/j.yjmcc.2010.02.017

MacDougall D, Colyer J, Calaghan S Caveolae Differentially Control Phosphorylation of Sarcoplasmic Reticular Proteins Following beta(2) Adrenoceptor Stimulation in the Adult Cardiac Myocyte, 2010

Colyer J Analyzing Protein Phosphorylation PROTEIN PROTOCOLS HANDBOOK, THIRD EDITION 1547-1553, 2009
DOI:10.1007/978-1-59745-198-7_160

Locate S, Colyer J, Gawler DJ, Walker JH Annexin A6 at the cardiac myocyte sarcolemma - Evidence for self-association and binding to actin CELL BIOL INT 32 1388-1396, 2008
DOI:10.1016/j.cellbi.2008.08.009

Barfoot RJ, Sheikh KH, Johnson BRG, Colyer J, Miles RE, Jeuken LJC, Bushby RJ, Evans SD Minimal F-actin cytoskeletal system for planar supported phospholipid bilayers LANGMUIR 24 6827-6836, 2008
DOI:10.1021/la800085n

Johnson BRG, Bushby RJ, Colyer J, Evans SD Self-assembly of actin scaffolds at ponticulin containing supported phospholipid bilayers.(vol 90, pg L21, 2006) BIOPHYS J 93 704-704, 2007
DOI:10.1529/biophysj.107.0900160

Johnson BRG, Bushby RJ, Colyer J, Evans SD Self-assembly of actin scaffolds at ponticulin containing supported phospholipid bilayers.(vol 90, pg L21, 2006) BIOPHYSICAL JOURNAL 93 704-704, 2007
DOI:10.1529/biophysj.107.0900160

Jones PP, Bazzazi H, Kargacin GJ, Colyer J Inhibition of cAMP-dependent protein kinase under conditions occurring in the cardiac dyad during a Ca2+ transient BIOPHYS J 91 433-443, 2006
DOI:10.1529/biophysj.106.083931

Carter S, Colyer J, Sitsapesan R Maximum phosphorylation of the cardiac ryanodine receptor at serine-2809 by protein kinase A produces unique modifications to channel gating and conductance not observed at lower levels of phosphorylation CIRC RES 98 1506-1513, 2006
DOI:10.1161/01.RES.0000227506.43292.df

Johnson BRG, Bushby RJ, Colyer J, Evans SD Assembly of actin scaffolds at ponticulin-containing supported phospholipid bilayers BIOPHYS J 90 L21-L23, 2006
DOI:10.1529/biophysj.105.076521

Rodriguez P, Jackson WA, Colyer J Critical evaluation of cardiac Ca2+-ATPase phosphorylation on serine 38 using a phosphorylation site-specific antibody J BIOL CHEM 279 17111-17119, 2004
DOI:10.1074/jbc.M400462200

Brette F, Rodriguez P, Komukai K, Colyer J, Orchard CH β-adrenergic stimulation restores the Ca transient of ventricular myocytes lacking t-tubules Journal of Molecular and Cellular Cardiology 36 265-275, 2004
DOI:10.1016/j.yjmcc.2003.11.002
View abstract

Brette FP, Rodriguez P, Komukai K, Colyer J, Orchard CH beta-adrenergic stimulation restores the Ca transient of ventricular myocytes lacking t-tubules. Journal of Molecular and Cellular Cardiology 36 265-275, 2004
DOI:10.1016/j.yjmcc.2003.11.002

Rodriguez P, Bhogal MS, Colyer J Stoichiometric Phosphorylation of Cardiac Ryanodine Receptor on Serine 2809 by Calmodulin-dependent Kinase II and Protein Kinase A Journal of Biological Chemistry 278 38593-38600, 2003
DOI:10.1074/jbc.C301180200

Jones PP, Colyer J Identification of state of filling kinase from cardiac SR Biophysical Journal 84 99A-99A, 2003

Rodriguez P, Bhogal MS, Colyer J RYR2 phosphorylation: can PKA phosphorylate the ryanodine receptor at Ser-2809? Biophysical Journal 84 pp.99-, 2003

Bhogal MS, Colyer J Phospholamban degradation: Routes and rates of degradation in model eukaryotic cells Biophysical Journal 84 101A-101A, 2003

Ng YL, Goldspink DF, Burniston JG, Clark WA, Colyer J, Tan LB Characterisation of isoprenaline myotoxicity on slow-twitch skeletal versus cardiac muscle INT J CARDIOL 86 299-309, 2002

Brette FP, Rodriguez P, Colyer J, Orchard CH Effect of detubulation on phospholamban phosphorylation in rat ventricular myocytes Journal of Physiology 544 46-46, 2002

Burniston JG, Ng Y, Clark WA, Colyer J, Tan LB, Goldspink DF Myotoxic effects of clenbuterol in the rat heart and soleus muscle Journal of Applied Physiology 93 1824-1832, 2002

Locate S, Adegunloye BJ, Colyer J, Walker JH Annexin VI binds to calsequestrin of skeletal muscle sarcoplasmic reticulum FASEB J 15 A816-A816, 2001

Bhogal MS, Colyer J The rate of phospholamban degradation in cultured cardiac myocytes BIOPHYS J 80 586A-587A, 2001

Aly A, Hunter M, Knowles PF, Colyer J Phospholamban exhibits regulated ion channel activity BIOPHYS J 80 26A-26A, 2001

McDonald RL, Colyer J, Harrison SM Quantitative analysis of Na+-Ca2+ exchanger expression in guinea-pig heart. Eur J Biochem 267 5142-5148, 2000
View abstract

Brette FP, Calaghan SC, Lappin S, White E, Colyer J, Le Guennec JY Biphasic effects of hypo-osmotic challenge on excitation-contraction coupling in rat ventricular myocytes American Journal of Physiology 279 1963-1971, 2000

McDonald RL, Colyer J, Harrison SM Regional variations in the location of Na-Ca exchanger protein in guinea-pig heart at cellular and tissue levels BRIT J PHARMACOL 128 U19-U19, 1999

McDonald RL, Drago GA, Colyer J, Harrison SM Expression of Na+-Ca2+ exchanger in guinea-pig left ventricle BIOPHYS J 76 A305-A305, 1999

Calaghan SC, Colyer J, White E Cyclic AMP but not phosphorylation of phospholamban increase during the slow response to stretch in ferret papillary muscle. Pflugers Archiv: European Journal of Physiology 437 780-782, 1999

Hussain M, Drago GA, Bhogal MS, Colyer J, Orchard CH Effects of the protein kinase inhibitor H-89 on Ca2+ regulation in isolazted ferret ventricular myocytes Pflugers Archiv: European Journal of Physiology 437 529-537, 1999

Levine BA, Patchell VB, Sharma P, Gao Y, Bigelow DJ, Yao Q, Goh S, Colyer J, Drago GA, Perry SV Sites on the cytoplasmic region of phospholamban involved in interaction with the calcium-activated ATPase of the sarcoplasmic reticulum European Journal of Biochemistry 264 905-913, 1999

Colyer J Phosphorylation states of phospholamban. Ann N Y Acad Sci 853 79-91, 1998
View abstract

Bhogal MS, Colyer J Depletion of sarcoplasmic reticulum calcium prompts phosphorylation of phospholamban to stimulate store refilling. Ann N Y Acad Sci 853 260-263, 1998

Drago GA, Colyer J, Lederer WJ Immunofluorescence localization of SERCA2a and the phosphorylated forms of phospholamban in intact rat cardiac ventricular myocytes. Ann N Y Acad Sci 853 273-279, 1998

Ng Y, Colyer J, Tan LB, Clark WA, Goldspink DF Rat striated muscles damaged by isoprenaline J PHYSIOL-LONDON 511P 157P-158P, 1998

Calaghan SC, Colyer J, White E Length-dependent modulation of cAMP and the phosphorylation of phospholamban in isolated ferret papillary muscle J PHYSIOL-LONDON 511P 82P-83P, 1998

Palfreyman T, Hussain M, Drago GA, Berrebi-Bertrand I, Bril A, Orchard CH, Colyer J The importance of protein phosphatase 1 in cardiac CA(2+) homeostasis BIOPHYS J 74 A379-A379, 1998

Colyer J Chemical Modification, 1998

Calaghan SC, White E, Colyer J Preservation of the in vivo phosphorylation status of phospholamban in the heart: Evidence for an in situ difference in the dephosphorylation of phospholamban Biochemical and Biophysical Research Communications 248 701-705, 1998

Calaghan SC, White E, Colyer J Co-ordinated changes in cAMP, phosphorylation of phospholamban, Ca2+, and contraction following beta-adrenergic stimulation of rat heart. Pflugers Archiv: European Journal of Physiology 436 948-956, 1998

Bhogal MS, Colyer J Depletion of Ca2+ from the sarcoplasmic reticulum of cardiac muscle prompts phosphorylation of phospholamban to stimulate store refilling Proceedings of the National Academy of Sciences of the United States of America 95 1484-1489, 1998

Ng Y, Wright H, Colyer J, Clark WA, Tan LB, Goldspink DF Localization of isoprenaline induced damage in myocytes of striated muscle. J PHYSIOL-LONDON 504P P74-P74, 1997

Hussain M, Orchard CH, Colyer J Effects of the protein kinase A (PKA) inhibitor H89 on the Ca2+ current (I-Ca) and the Ca2+ transient in isolated ferret ventricular myocytes. J PHYSIOL-LONDON 504P P79-P80, 1997

Hulme JT, Colyer J, Orchard CH Acidosis alters the phosphorylation of Ser16 and Thr17 of phospholamban in rat cardiac muscle Pflugers Archiv: European Journal of Physiology 434 475-483, 1997

Hussain M, Drago GA, Colyer J, Orchard CH Rate-dependent abbreviation of Ca2+ transient in rat heart is independent of phospholamban phosphorylation American Journal of Physiology: Heart and Circulatory Physiology 42 695-706, 1997

Calaghan SC, White E, Colyer J Quantitative analysis of the phosphorylation of phospholamban in isolated ferret papillary muscle J PHYSIOL-LONDON 497P P3-P4, 1996

Hussain M, Drago GA, Colyer J, Orchard CH Inhibitors of Ca2+-calmodulin-dependent protein kinase II (CaM kinase II) do not prevent rate-dependent abbreviation of the Ca2+ transient in isolated rat ventricular myocytes J PHYSIOL-LONDON 497P P49-P49, 1996

Drago GA, Hussain M, Colyer J Temporal resolution of phospholamban phosphorylation and dephosphorylation in vivo FASEB J 10 1810-1810, 1996

Quirk PG, Patchell VB, Colyer J, Drago GA, Gao Y Conformational effects of serine phosphorylation in phospholamban peptides EUR J BIOCHEM 236 85-91, 1996

Jackson WA, Colyer J Translation of Ser16 and Thr17 phosphorylation of phospholamban into Ca2+-pump stimulation Biochemical Journal 316 201-207, 1996

YANG ZK, BOYETT MR, JANVIER NC, HULME JT, ORCHARD CH, COLYER J DIFFERENCES IN THE TIME-COURSE OF CONTRACTION IN SUB-EPICARDIAL AND SUB-ENDOCARDIAL CELLS ISOLATED FROM THE LEFT-VENTRICLE OF THE DOG AND FERRET J PHYSIOL-LONDON 487P P127-P127, 1995

HULME JT, COLYER J, ORCHARD CH THE EFFECTS OF ACIDOSIS ON THE PHOSPHORYLATION OF SER(16) AND THR(17) OF PHOSPHOLAMBAN IN RAT CARDIAC-MUSCLE J PHYSIOL-LONDON 487P P128-P129, 1995

BENNETT KL, WHITE E, COLYER J THE EFFECT OF CHANGES IN MECHANICAL LOADING ON THE PHOSPHORYLATION OF PHOSPHOLAMBAN IN ISOLATED FERRET PAPILLARY-MUSCLE J PHYSIOL-LONDON 487P P141-P141, 1995

BENNETT KL, COLYER J, ORCHARD CH THE EFFECT OF STIMULATION RATE AND BATHING [CA2+] ON THE PHOSPHORYLATION OF PHOSPHOLAMBAN IN ISOLATED RAT VENTRICULAR MYOCYTES J PHYSIOL-LONDON 483P P20-P20, 1995

Smith FK, Colyer J Expression and purification of cardiac phospholamban in Escherichia coli. Biochem Soc Trans 23 22S-, 1995

Jackson WA, Colyer J Release of Ca(2+)-Mg2+ ATPase inhibition by phospholamban phosphorylation. Biochem Soc Trans 23 62S-, 1995

Drago GA, Colyer J Discrimination between two sites of phosphorylation on adjacent amino acids by phosphorylation site-specific antibodies to phospholamban. J Biol Chem 269 25073-25077, 1994
View abstract

Colyer J Control of the calcium pump of cardiac sarcoplasmic reticulum. A specific role for the pentameric structure of phospholamban? Cardiovasc Res 27 1766-1771, 1993
View abstract

Colyer J, Wang JH Dependence of cardiac sarcoplasmic reticulum calcium pump activity on the phosphorylation status of phospholamban. J Biol Chem 266 17486-17493, 1991
View abstract

Morris GL, Cheng HC, Colyer J, Wang JH Phospholamban regulation of cardiac sarcoplasmic reticulum (Ca(2+)-Mg2+)-ATPase. Mechanism of regulation and site of monoclonal antibody interaction. J Biol Chem 266 11270-11275, 1991
View abstract

Mata AM, Colyer J, Michelangeli F, Lee AG, East JM The effect of a monoclonal antibody on specific steps of the reaction sequence of the (Ca(2+)-Mg(2+))-ATPase from sarcoplasmic reticulum. Biochem Soc Trans 19 S205-S205, 1991

MOVSESIAN MA, LEVEILLE C, KRALL J, COLYER J, WANG JH, CAMPBELL KP IDENTIFICATION AND CHARACTERIZATION OF PROTEINS IN SARCOPLASMIC-RETICULUM FROM NORMAL AND FAILING HUMAN LEFT-VENTRICLES J MOL CELL CARDIOL 22 1477-1485, 1990

Li CF, Wang JH, Colyer J Immunological detection of phospholamban phosphorylation states facilitates the description of the mechanism of phosphorylation and dephosphorylation. Biochemistry 29 4535-4540, 1990
View abstract

Michelangeli F, Colyer J, East JM, Lee AG Effect of pH on the activity of the Ca2+ + Mg2(+)-activated ATPase of sarcoplasmic reticulum. Biochem J 267 423-429, 1990
View abstract

BURGOYNE RD, CHEEK TR, MORGAN A, OSULLIVAN AJ, MORETON RB, BERRIDGE MJ, MATA AM, COLYER J, LEE AG, EAST JM DISTRIBUTION OF 2 DISTINCT CA-2+-ATPASE-LIKE PROTEINS AND THEIR RELATIONSHIPS TO THE AGONIST-SENSITIVE CALCIUM STORE IN ADRENAL CHROMAFFIN CELLS NATURE 342 72-74, 1989

Colyer J, Mata AM, Lee AG, East JM Effects on ATPase activity of monoclonal antibodies raised against (Ca2+ + Mg2+)-ATPase from rabbit skeletal muscle sarcoplasmic reticulum and their correlation with epitope location. Biochem J 262 439-447, 1989
View abstract

Matthews I, Colyer J, Mata AM, Green NM, Sharma RP, Lee AG, East JM Evidence for the cytoplasmic location of the N- and C-terminal segments of sarcoplasmic reticulum (Ca2+-Mg2+)-ATPase. Biochem Biophys Res Commun 161 683-688, 1989
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Kumar PJ, Walker-Smith J, Milla P, Harris G, Colyer J, Halliday R The teenage coeliac: follow up study of 102 patients. Arch Dis Child 63 916-920, 1988
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COLYER J, KUMAR PJ, WALDRON NM, CLARK ML, FARTHING MJG GLIADIN BINDING TO RAT AND HUMAN ENTEROCYTES CLIN SCI 72 593-598, 1987

COLYER J, FARTHING MJG, KUMAR PJ, CLARK ML, OHANNESIAN AD, WALDRON NM REAPPRAISAL OF THE LECTIN HYPOTHESIS IN THE ETIOPATHOGENESIS OF CELIAC-DISEASE CLIN SCI 71 105-110, 1986