Faculty of Biological Sciences

Dr David Brockwell

BSc, PhD 1997, Manchester.
Associate Professor
School of Molecular and Cellular Biology

Contact:  Astbury 10.116 | +44(0) 113 34 37821 | email address for Dr David Brockwell 

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Admin roles

UG Examinations Officer
Wellcome Trust Module Manager

Modules managed

BIOC1303 - Intro Biochem Problem Solving

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC1302 - Intro Biochem Practical Skills
BIOC1303 - Intro Biochem Problem Solving
BIOC2301 - Interm Integrated Biochemistry
BIOC2302 - Intermediate Biochem: Prac
BIOC2303 - Intermediate Biochem: Skills
BIOC3160 - Lab/Lit/Comp Research Project
BIOC3221/22/23 b - ATU 28 - protein dynamics
BIOC3221/22/23/BIOL3210 - ATU 09 - Folding disorders
BIOL1112 - The Molecules of Life
BIOL3306 - Biol Sciences Research Project
BIOL3399 - Extended Research Project Preparation
BIOW5901X - Foundation module
BIOW5908X - Emerging Techniques
BMSC1103 - Basic Laboratory and Scientific Skills
BMSC2120 - Scientific Skills


Member of Undergraduate School Taught Student Education Committee

McMorran LM; Brockwell DJ; Radford SE Mechanistic studies of the biogenesis and folding of outer membrane proteins in vitro and in vivo: What have we learned to date? Archives of Biochemistry and Biophysics -, 2014
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Farrance OE; Hann E; Kaminska R; Housden NG; Derrington SR; Kleanthous C; Radford SE; Brockwell DJ A force-activated trip switch triggers rapid dissociation of a colicin from its immunity protein. PLoS Biol 11 e1001489-, 2013
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McMorran LM; Bartlett AL; Huysmans GHM; Radford SE; Brockwell DJ Dissecting the effects of periplasmic chaperones on the in vitro folding of the outer membrane protein PagP Journal of Molecular Biology 425 3178-3191, 2013
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Hoffmann T; Tych KM; Hughes ML; Brockwell DJ; Dougan L Towards design principles for determining the mechanical stability of proteins Physical Chemistry Chemical Physics 15 15767-15780, 2013
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Tych KM; Hoffmann T; Brockwell DJ; Dougan L Single molecule force spectroscopy reveals the temperature-dependent robustness and malleability of a hyperthermophilic protein Soft Matter 9 9016-9025, 2013
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Huysmans GH; Radford SE; Baldwin SA; Brockwell DJ Malleability of the folding mechanism of the outer membrane protein PagP: parallel pathways and the effect of membrane elasticity. J Mol Biol 416 453-464, 2012
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Ikeda-Kobayashi A; Taniguchi Y; Kawakami M; Brockwell DJ; Paci E Prying open single GroES Ring complexes by force reveals cooperativity across domains Biophysical Journal 102 1961-1968, 2012
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Crampton N; Alzahrani K; Beddard GS; Connell SD; Brockwell DJ Mechanically Unfolding Protein L Using a Laser-Feedback-Controlled Cantilever BIOPHYS J 100 1800-1809, 2011

Crampton N; Brockwell DJ Unravelling the design principles for single protein mechanical strength CURR OPIN STRUC BIOL 20 508-517, 2010

Beddard GS; Brockwell DJ A statistical approach to the estimation of mechanical unfolding parameters from the unfolding patterns of protein heteropolymers PHYS BIOL 7 -, 2010

Pugh SD; Gell C; Smith DA; Radford SE; Brockwell DJ Single-Molecule Studies of the Im7 Folding Landscape J MOL BIOL 398 132-145, 2010

Huysmans GHM; Baldwin SA; Brockwell DJ; Radford SE The transition state for folding of an outer membrane protein P NATL ACAD SCI USA 107 4099-4104, 2010

Taniguchi Y; Khatri BS; Brockwell DJ; Paci E; Kawakami M Dynamics of the Coiled-Coil Unfolding Transition of Myosin Rod Probed by Dissipation Force Spectrum BIOPHYS J 99 257-262, 2010

Sadler DP; Petrik E; Taniguchi Y; Pullen JR; Kawakami M; Radford SE; Brockwell DJ Identification of a Mechanical Rheostat in the Hydrophobic Core of Protein L J MOL BIOL 393 237-248, 2009

Grant CA; Brockwell DJ; Radford SE; Thomson NH Tuning the Elastic Modulus of Hydrated Collagen Fibrils BIOPHYS J 97 2985-2992, 2009

Anderson KL; Radford SE; Smith DA; Brockwell DJ The dynamical response of proteins under force In Handbook of molecular force spectroscopy , 2008

Khatri BS; Byrne K; Kawakami M; Brockwell DJ; Smith DA; Radford SE; McLeish TCB Internal friction of single polypeptide chains at high stretch FARADAY DISCUSS 139 35-51, 2008

Grant CA; Brockwell DJ; Radford SE; Thomson NH Effects of hydration on the mechanical response of individual collagen fibrils APPL PHYS LETT 92 -, 2008

Taniguchi Y; Brockwell DJ; Kawakami M The Effect of Temperature on Mechanical Resistance of the Native and Intermediate States of I27 BIOPHYS J 95 5296-5305, 2008

Huysmans GH; Radford SE; Brockwell DJ; Baldwin SA The N-terminal helix is a post-assembly clamp in the bacterial outer membrane protein PagP. J Mol Biol 373 529-540, 2007
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Brockwell DJ Probing the mechanical stability of proteins using the atomic force microscope BIOCHEM SOC T 35 1564-1568, 2007

Brockwell DJ; Radford SE Intermediates: ubiquitous species on folding energy landscapes? CURR OPIN STRUC BIOL 17 30-37, 2007

Brockwell DJ Force denaturation of proteins - an unfolding story Current Nanoscience 3 3-15, 2007
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Hann E; Kirkpatrick N; Kleanthous C; Smith DA; Radford SE; Brockwell DJ The effect of protein complexation on the mechanical stability of Im9 BIOPHYS J 92 L79-L81, 2007

Tezuka-Kawakami T; Gell C; Brockwell DJ; Radford SE; Smith DAM Urea-Induced Unfolding of the Immunity Protein Im9 Monitored by spFRET Biophysical Journal 91 L42-L44, 2006
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West DK; Brockwell DJ; Olmsted PD; Radford SE; Paci E Mechanical resistance of proteins explained using simple molecular models BIOPHYS J 90 287-297, 2006

West DK; Brockwell DJ; Paci E Prediction of the translocation kinetics of a protein from its mechanical properties BIOPHYS J 91 L51-L53, 2006

Kawakami M; Byrne K; Brockwell DJ; Radford SE; Smith DA Viscoelastic study of the mechanical unfolding of a protein by AFM Biophysical Journal 91 L16-L18, 2006
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Brockwell DJ; Beddard GS; Paci E; West DK; Olmsted PD; Smith DA; Radford SE Mechanically unfolding the small, topologically simple protein L BIOPHYS J 89 506-519, 2005

Best RB; Brockwell DJ; Toca-Herrera JL; Blake AW; Smith DA; Radford SE; Clarke J Force mode atomic force microscopy as a tool for protein folding studies ANAL CHIM ACTA 479 87-105, 2003

Brockwell DJ; Paci E; Zinober RC; Beddard GS; Olmsted PD; Smith DA; Perham RN; Radford SE Pulling geometry defines the mechanical resistance of a beta-sheet protein. Nat Struct Biol 10 731-737, 2003
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Smith DAM; Brockwell DJ; Zinober RC; Blake AW; Beddard GS; Olmsted PD; Radford SE Unfolding dynamics of proteins under applied force Philosophical Transactions of the Royal Society of London, Series A: Mathematical, Physical and Engineering Sciences 361 739-740, 2003

Alastair Smith D; Zinober RC; Olmsted PD; Brockwell DJ; Blake AW; Radford SE; Beddard GS; Coffey WT; Sackmann E; Felderhof BU; Maaloum M; Titmuss S; Crothers DSF; Bartlett P Unfolding dynamics of proteins under applied force Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 361 713-730, 2003
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Zinober RC; Brockwell DJ; Beddard GS; Blake AW; Olmsted PD; Radford SE; Smith DA Mechanically unfolding proteins: The effect of unfolding history and the supramolecular scaffold PROTEIN SCI 11 2759-2765, 2002

Brockwell DJ; Beddard GS; Clarkson J; Zinober RC; Blake AW; Trinick J; Olmsted PD; Smith DAM; Radford SE The Effect of Core Destabilization on the Mechanical Resistance of I27 Biophysical Journal 83 458-472, 2002

Gell C; Brockwell DJ; Beddard GS; Radford SE; Kalvarda A; Smith DAM Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times Single Molecules 2 177-182, 2001

Gell C; Brockwell DJ; Beddard GS; Radford SE; Kalverda AP; Smith DA Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times SINGLE MOL 2 177-181, 2001

Brockwell DJ; Smith DAM; Radford SE Protein folding mechanisms: new methods and emerging ideas Current Opinion in Structural Biology 16-25, 2000

Gell C; Brockwell D; Smith A Handbook of single molecule fluorescence spectroscopy,

Hoffmann T; Tych KM; Brockwell DJ; Dougan L Single Molecule Force Spectroscopy Identifies a Small Cold Shock Protein as Being Mechanically Robust The Journal of Physical Chemistry B 0 null-null,
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