Faculty of Biological Sciences

Dr David Brockwell

BSc, PhD 1997, Manchester.
Associate Professor
School of Molecular and Cellular Biology

Contact:  Astbury 10.116 | +44(0) 113 34 37821 | email address for Dr David Brockwell 

You can read more about Dr Brockwell's interests here:
www.fbs.leeds.ac.uk/staff/Brockwell_DJ

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Admin roles

FBS UGTSEC
UG Examinations Officer
Wellcome Trust Module Manager

Modules managed

BIOC1303 - Intro Biochem Problem Solving

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC1302 - Intro Biochem Practical Skills
BIOC1303 - Intro Biochem Problem Solving
BIOC2301 - Interm Integrated Biochemistry
BIOC2302 - Intermediate Biochem: Prac
BIOC2303 - Intermediate Biochem: Skills
BIOC3160 - Lab/Lit/Comp Research Project
BIOC3221/22/23 b - ATU 28 - protein dynamics
BIOC3221/22/23/BIOL3210 - ATU 09 - Folding disorders
BIOL1112 - The Molecules of Life
BIOL3306 - Biol Sciences Research Project
BIOL3399 - Extended Research Project Preparation
BIOW5901X - Foundation module
BIOW5908X - Emerging Techniques
BMSC1103 - Basic Laboratory and Scientific Skills
BMSC2120 - Scientific Skills

Committees

Member of Undergraduate School Taught Student Education Committee

McMorran LM; Brockwell DJ; Radford SE Mechanistic studies of the biogenesis and folding of outer membrane proteins in vitro and in vivo: What have we learned to date? Archives of Biochemistry and Biophysics -, 2014
DOI:10.1016/j.abb.2014.02.011
View abstract

Farrance OE; Hann E; Kaminska R; Housden NG; Derrington SR; Kleanthous C; Radford SE; Brockwell DJ A force-activated trip switch triggers rapid dissociation of a colicin from its immunity protein. PLoS Biol 11 e1001489-, 2013
DOI:10.1371/journal.pbio.1001489
View abstract

McMorran LM; Bartlett AL; Huysmans GHM; Radford SE; Brockwell DJ Dissecting the effects of periplasmic chaperones on the in vitro folding of the outer membrane protein PagP Journal of Molecular Biology 425 3178-3191, 2013
DOI:10.1016/j.jmb.2013.06.017
View abstract

Hoffmann T; Tych KM; Hughes ML; Brockwell DJ; Dougan L Towards design principles for determining the mechanical stability of proteins Physical Chemistry Chemical Physics 15 15767-15780, 2013
DOI:10.1039/c3cp52142g
View abstract

Tych KM; Hoffmann T; Brockwell DJ; Dougan L Single molecule force spectroscopy reveals the temperature-dependent robustness and malleability of a hyperthermophilic protein Soft Matter 9 9016-9025, 2013
DOI:10.1039/C3SM51439K
View abstract

Huysmans GH; Radford SE; Baldwin SA; Brockwell DJ Malleability of the folding mechanism of the outer membrane protein PagP: parallel pathways and the effect of membrane elasticity. J Mol Biol 416 453-464, 2012
DOI:10.1016/j.jmb.2011.12.039
View abstract

Ikeda-Kobayashi A; Taniguchi Y; Kawakami M; Brockwell DJ; Paci E Prying open single GroES Ring complexes by force reveals cooperativity across domains Biophysical Journal 102 1961-1968, 2012
DOI:10.1016/j.bpj.2012.03.046
View abstract

Crampton N; Alzahrani K; Beddard GS; Connell SD; Brockwell DJ Mechanically Unfolding Protein L Using a Laser-Feedback-Controlled Cantilever BIOPHYS J 100 1800-1809, 2011
DOI:10.1016/j.bpj.2011.02.021

Crampton N; Brockwell DJ Unravelling the design principles for single protein mechanical strength CURR OPIN STRUC BIOL 20 508-517, 2010
DOI:10.1016/j.sbi.2010.05.005

Beddard GS; Brockwell DJ A statistical approach to the estimation of mechanical unfolding parameters from the unfolding patterns of protein heteropolymers PHYS BIOL 7 -, 2010
DOI:10.1088/1478-3975/7/1/014001

Pugh SD; Gell C; Smith DA; Radford SE; Brockwell DJ Single-Molecule Studies of the Im7 Folding Landscape J MOL BIOL 398 132-145, 2010
DOI:10.1016/j.jmb.2010.02.048

Huysmans GHM; Baldwin SA; Brockwell DJ; Radford SE The transition state for folding of an outer membrane protein P NATL ACAD SCI USA 107 4099-4104, 2010
DOI:10.1073/pnas.0911904107

Taniguchi Y; Khatri BS; Brockwell DJ; Paci E; Kawakami M Dynamics of the Coiled-Coil Unfolding Transition of Myosin Rod Probed by Dissipation Force Spectrum BIOPHYS J 99 257-262, 2010
DOI:10.1016/j.bpj.2010.04.007

Sadler DP; Petrik E; Taniguchi Y; Pullen JR; Kawakami M; Radford SE; Brockwell DJ Identification of a Mechanical Rheostat in the Hydrophobic Core of Protein L J MOL BIOL 393 237-248, 2009
DOI:10.1016/j.jmb.2009.08.015

Grant CA; Brockwell DJ; Radford SE; Thomson NH Tuning the Elastic Modulus of Hydrated Collagen Fibrils BIOPHYS J 97 2985-2992, 2009
DOI:10.1016/j.bpj.2009.09.010

Anderson KL; Radford SE; Smith DA; Brockwell DJ The dynamical response of proteins under force In Handbook of molecular force spectroscopy , 2008

Khatri BS; Byrne K; Kawakami M; Brockwell DJ; Smith DA; Radford SE; McLeish TCB Internal friction of single polypeptide chains at high stretch FARADAY DISCUSS 139 35-51, 2008
DOI:10.1039/b716418c

Grant CA; Brockwell DJ; Radford SE; Thomson NH Effects of hydration on the mechanical response of individual collagen fibrils APPL PHYS LETT 92 -, 2008
DOI:10.1063/1.2937001

Taniguchi Y; Brockwell DJ; Kawakami M The Effect of Temperature on Mechanical Resistance of the Native and Intermediate States of I27 BIOPHYS J 95 5296-5305, 2008
DOI:10.1529/biophysj.108.141275

Huysmans GH; Radford SE; Brockwell DJ; Baldwin SA The N-terminal helix is a post-assembly clamp in the bacterial outer membrane protein PagP. J Mol Biol 373 529-540, 2007
DOI:10.1016/j.jmb.2007.07.072
View abstract

Brockwell DJ Probing the mechanical stability of proteins using the atomic force microscope BIOCHEM SOC T 35 1564-1568, 2007
DOI:10.1042/BST0351564

Brockwell DJ; Radford SE Intermediates: ubiquitous species on folding energy landscapes? CURR OPIN STRUC BIOL 17 30-37, 2007
DOI:10.1016/j.sbi.2007.01.003

Brockwell DJ Force denaturation of proteins - an unfolding story Current Nanoscience 3 3-15, 2007
View abstract

Hann E; Kirkpatrick N; Kleanthous C; Smith DA; Radford SE; Brockwell DJ The effect of protein complexation on the mechanical stability of Im9 BIOPHYS J 92 L79-L81, 2007
DOI:10.1529/biophysj.106.102475

Tezuka-Kawakami T; Gell C; Brockwell DJ; Radford SE; Smith DAM Urea-Induced Unfolding of the Immunity Protein Im9 Monitored by spFRET Biophysical Journal 91 L42-L44, 2006
DOI:10.1529/biophysj.106.088344
View abstract

West DK; Brockwell DJ; Olmsted PD; Radford SE; Paci E Mechanical resistance of proteins explained using simple molecular models BIOPHYS J 90 287-297, 2006
DOI:10.1529/biophysj.105.071035

West DK; Brockwell DJ; Paci E Prediction of the translocation kinetics of a protein from its mechanical properties BIOPHYS J 91 L51-L53, 2006
DOI:10.1529/biophysj.106.089490

Kawakami M; Byrne K; Brockwell DJ; Radford SE; Smith DA Viscoelastic study of the mechanical unfolding of a protein by AFM Biophysical Journal 91 L16-L18, 2006
DOI:10.1529/biophysj.106.085019
View abstract

Brockwell DJ; Beddard GS; Paci E; West DK; Olmsted PD; Smith DA; Radford SE Mechanically unfolding the small, topologically simple protein L BIOPHYS J 89 506-519, 2005
DOI:10.1529/biophysj.105.061465

Best RB; Brockwell DJ; Toca-Herrera JL; Blake AW; Smith DA; Radford SE; Clarke J Force mode atomic force microscopy as a tool for protein folding studies ANAL CHIM ACTA 479 87-105, 2003
DOI:10.1016/S0003-2670(02)01572-6

Brockwell DJ; Paci E; Zinober RC; Beddard GS; Olmsted PD; Smith DA; Perham RN; Radford SE Pulling geometry defines the mechanical resistance of a beta-sheet protein. Nat Struct Biol 10 731-737, 2003
DOI:10.1038/nsb968
View abstract

Smith DAM; Brockwell DJ; Zinober RC; Blake AW; Beddard GS; Olmsted PD; Radford SE Unfolding dynamics of proteins under applied force Philosophical Transactions of the Royal Society of London, Series A: Mathematical, Physical and Engineering Sciences 361 739-740, 2003

Alastair Smith D; Zinober RC; Olmsted PD; Brockwell DJ; Blake AW; Radford SE; Beddard GS; Coffey WT; Sackmann E; Felderhof BU; Maaloum M; Titmuss S; Crothers DSF; Bartlett P Unfolding dynamics of proteins under applied force Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences 361 713-730, 2003
DOI:10.1098/rsta.2002.1160
View abstract

Zinober RC; Brockwell DJ; Beddard GS; Blake AW; Olmsted PD; Radford SE; Smith DA Mechanically unfolding proteins: The effect of unfolding history and the supramolecular scaffold PROTEIN SCI 11 2759-2765, 2002
DOI:10.1110/ps.0224602

Brockwell DJ; Beddard GS; Clarkson J; Zinober RC; Blake AW; Trinick J; Olmsted PD; Smith DAM; Radford SE The Effect of Core Destabilization on the Mechanical Resistance of I27 Biophysical Journal 83 458-472, 2002

Gell C; Brockwell DJ; Beddard GS; Radford SE; Kalvarda A; Smith DAM Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times Single Molecules 2 177-182, 2001
DOI:10.1002/1438-5171(200110)2:3<177::AID-SIMO177>3.0.CO;2-4

Gell C; Brockwell DJ; Beddard GS; Radford SE; Kalverda AP; Smith DA Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times SINGLE MOL 2 177-181, 2001

Brockwell DJ; Smith DAM; Radford SE Protein folding mechanisms: new methods and emerging ideas Current Opinion in Structural Biology 16-25, 2000

Gell C; Brockwell D; Smith A Handbook of single molecule fluorescence spectroscopy,

Hoffmann T; Tych KM; Brockwell DJ; Dougan L Single Molecule Force Spectroscopy Identifies a Small Cold Shock Protein as Being Mechanically Robust The Journal of Physical Chemistry B 0 null-null,
DOI:10.1021/jp310442s
View abstract