Faculty of Biological Sciences

Prof Alison Ashcroft

BSc, Liverpool; MSc, PhD 1981, Manchester
Professor in Biomolecular Mass Spectrometry
School of Molecular and Cellular Biology

Background: CChem. FRSC. Research Fellow (University of Geneva), 1981-2; Applications Laboratory Manager/Senior Development Mass Spectrometrist, Kratos Analytical Ltd., 1983-8; Senior Mass Spectrometrist, Safety of Medicines Dept., ICI Pharmaceuticals, 1988-1990; Senior Applications Mass Spectrometrist, Micromass UK Ltd., 1991-1997.

Contact:  Astbury 8.110 | +44(0) 113 34 37273 | email address for  

You can read more about Prof Ashcroft's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=AEA


Research Interests

Mass spectrometry, mass spectrometry facility manager

The Mass Spectrometry (MS) Facility (http://www.astbury.leeds.ac.uk/facil/mass.htm) is located in a purpose-built suite in the Astbury Building (9.107) and has five ESI-MS instruments including: three Synapt mass spectrometers with travelling wave IMS (BBSRC-REI, SRIF4, and Waters UK Ltd. funded): an HDMS with a 32 K quadrupole for CID-MS/MS of high m/z species, an HDMS with on-line HPLC (Ultimate 3000, Dionex) and CID-MS/MS, and a G2-S with high resolution and sensitivity. The other two instruments are a customised LCT Premier (Wellcome Trust funded) with the highest m/z range currently available (m/z 60,000) plus high Field Asymmetric waveform IMS (FAIMS), and a Platform II ESI-quadrupole instrument (Wellcome Trust funded).

The MS Facility runs an analytical service in addition to having an active research group (http://www.personal.leeds.ac.uk/~fbsmaspe/).

 

 

Current Projects

Prof. Ashcroft’s research is focused on developing and applying mass spectrometric techniques and methodologies to the analysis of biomolecules. Much of our work uses electrospray ionisation (ESI) non-covalent mass spectrometry (MS) coupled with ion mobility spectrometry (IMS) and thus we are able to measure the molecular mass and size (i.e. cross-sectional area) of individual biomolecules from within highly heterogeneous mixtures in a single experiment.

The research involves the development of mass spectrometric techniques to aid the structural elucidation of biomolecules and can be categorised into three main areas:

1. Protein folding

Our early ESI-IMS-MS studies on protein folding involved the separation of co-populated protein conformers and measuring their populations as the protein folds/unfolds. This work led to the Journal of the American Society for Mass Spectrometry’s “Ron Hites Award” in 2009 for “outstanding original research” in collaboration with Prof. Sheena Radford.

2. Virus capsid and baseplate assembly

Virus capsids and virus baseplates are macromolecular assemblies built from protein subunits. We are using ESI-IMS-MS to monitor the conformation properties of the proteins and their assembly pathways (in collaboration with Profs. Peter Stockley and Nicola Stonehouse and Dr John Barr in Leeds, and Prof Christian Cambillau (Marseille) and Dr Neil Ferguson (UC Dublin)).

3. Membrane protein analyses

Membrane proteins are challenging to analyse due to their insolubility in many solvents. We are currently developing new mass spectrometric techniques of delivering membrane proteins to the mass spectrometer whilst maintaining their native-like conformation e.g. in detergents, or amphipols (in collaboration with Prof. Jean-Luc Popot, Paris) in order to study their folding and function (in collaboration with Profs. Peter Henderson and Stephen Baldwin).

 

 

 

Faculty Research and Innovation


Studentship information

Undergraduate project topics:

  • Investigating protein folding and self-assembly by mass spectrometry

Postgraduate studentship areas:

  • The use of Mass Spectrometry in studying biological structure and function

See also:

Modules managed

BIOC2301 - Interm Integrated Biochemistry

Modules taught

BIOC1301 - Intro Inte Biochem Mol Process
BIOC1302 - Intro Biochem Practical Skills
BIOC1303 - Intro Biochem Problem Solving
BIOC2301 - Interm Integrated Biochemistry
BIOC2303 - Intermediate Biochem: Skills
BIOC3111/12/13 b - ATU 02 - Structural & MS techniques
BIOC3160 - Lab/Lit/Comp Research Project
BIOC3303 - Advanced Biochem: Skills
BIOL5272M - Advanced Biomolecular Technologies
BIOW5901X - Foundation module
BIOW5908X - Emerging Techniques
BMSC2120 - Scientific Skills
CHEM5315M - Emerging Topics in Chemical Biology

Committees

Member of Graduate School Committee

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Prof Alison Ashcroft  (Professor in Biomolecular Mass Spectrometry)

Mass spectrometry, mass spectrometry facility manager 


Dr James Ault  (Mass Spectrometry Facility Manager)

Mass Spectrometry Facility Manager 

Dr Antonio Calabrese  (Research Fellow)

Dr Negar Rajabi  (Research Fellow)


Postgraduates

Irshad Ahmad  
Helen Beeston  
Paul Devine  
Christopher Fisher  
Patrick Knight  
Thomas Watkinson  
Lydia Young  

Leney AC; Pashley CL; Scarff CA; Radford SE; Ashcroft AE Insights into the role of the beta-2 microglobulin D-strand in amyloid propensity revealed by mass spectrometry. Mol Biosyst 10 412-420, 2014
DOI:10.1039/c3mb70420c
View abstract

Preston GW; Radford SE; Ashcroft AE; Wilson AJ Analysis of amyloid nanostructures using photo-cross-linking: in situ comparison of three widely used photo-cross-linkers. ACS Chem Biol 9 761-768, 2014
DOI:10.1021/cb400731s
View abstract

Eves-van den Akker S; Lilley CJ; Ault JR; Ashcroft AE; Jones JT; Urwin PE The feeding tube of cyst nematodes: characterisation of protein exclusion. PLoS One 9 e87289-, 2014
DOI:10.1371/journal.pone.0087289
View abstract

Vörös J; Rautureau GJP; Urbanek A; O'Connor M; Ferguson N; Fisher HC; Ashcroft AE Large-scale production and structural and biophysical characterizations of the human hepatitis B virus polymerase Journal of Virology 88 2584-2599, 2014
DOI:10.1128/JVI.02575-13
View abstract

Shepherd DA; Ariza A; Edwards TA; Barr JN; Stonehouse NJ; Ashcroft AE Probing Bunyavirus N protein oligomerisation using mass spectrometry Rapid Communications in Mass Spectrometry 28 793-800, 2014
DOI:10.1002/rcm.6841
View abstract

Giusti F; Catoire LJ; Casiraghi M; Popot J-L; Rieger J; Qian S; Calabrese AN; Watkinson TG; Radford SE; Ashcroft AE Synthesis, Characterization and Applications of a Perdeuterated Amphipol The Journal of Membrane Biology -, 2014
DOI:10.1007/s00232-014-9656-x
View abstract

Pritchard C; Groves KJ; Biesenbruch S; Oconnor G; Quaglia M; Ashcroft AE; Arsene C; Schulze D Quantification of human growth hormone in serum with a labeled protein as an internal standard: Essential considerations Analytical Chemistry 86 6525-6532, 2014
DOI:10.1021/ac501032q
View abstract

Woods LA; Radford SE; Ashcroft AE Advances in ion mobility spectrometry-mass spectrometry reveal key insights into amyloid assembly. Biochim Biophys Acta 1834 1257-1268, 2013
DOI:10.1016/j.bbapap.2012.10.002
View abstract

Sarell CJ; Woods LA; Su Y; Debelouchina GT; Ashcroft AE; Griffin RG; Stockley PG; Radford SE Expanding the repertoire of amyloid polymorphs by co-polymerization of related protein precursors. J Biol Chem 288 7327-7337, 2013
DOI:10.1074/jbc.M112.447524
View abstract

Wilson AJ; Ault JR; Filby MH; Philips HI; Ashcroft AE; Fletcher NC Protein destabilisation by ruthenium(II) tris-bipyridine based protein-surface mimetics. Org Biomol Chem 11 2206-2212, 2013
DOI:10.1039/c3ob26251k
View abstract

Young L; Ndlovu H; Knapman TW; Harris SA; Radford SE; Ashcroft AE Monitoring oligomer formation from self-aggregating amylin peptides using ESI-IMS-MS International Journal for Ion Mobility Spectrometry 16 29-39, 2013
DOI:10.1007/s12127-012-0115-z
View abstract

Knapman TW; Warriner SL; Valette NM; Ashcroft AE Ion mobility spectrometry-mass spectrometry of intrinsically unfolded proteins: Trying to put order into disorder Current Analytical Chemistry 9 181-191, 2013
View abstract

Ariza A; Tanner SJ; Walter CT; Dent KC; Shepherd DA; Wu W; Matthews SV; Hiscox JA; Green TJ; Luo M; Elliott RM; Fooks AR; Ashcroft AE; Stonehouse NJ; Ranson NA; Barr JN; Edwards TA Nucleocapsid protein structures from orthobunyaviruses reveal insight into ribonucleoprotein architecture and RNA polymerization. Nucleic Acids Research 41 5912-5926, 2013
DOI:10.1093/nar/gkt268
View abstract

Pritchard C; O'Connor G; Ashcroft AE The Role of Ion Mobility Spectrometry-Mass Spectrometry in the Analysis of Protein Reference Standards ANALYTICAL CHEMISTRY 85 7205-7212, 2013
DOI:10.1021/ac400927s

Alexander CG; Juergens MC; Shepherd DA; Freund SMV; Ashcroft AE; Ferguson N Thermodynamic origins of protein folding, allostery, and capsid formation in the human hepatitis B virus core protein PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA 110 E2782-E2791, 2013
DOI:10.1073/pnas.1308846110

Ndlovu H; Ashcroft AE; Radford SE; Harris SA Molecular dynamics simulations of mechanical failure in polymorphic arrangements of amyloid fibrils containing structural defects Beilstein Journal of Nanotechnology 4 429-440, 2013
DOI:10.3762/bjnano.4.50
View abstract

Shepherd DA; Holmes K; Rowlands DJ; Stonehouse NJ; Ashcroft AE Using ion mobility spectrometry-mass spectrometry to decipher the conformational and assembly characteristics of the hepatitis B capsid protein. Biophys J 105 1258-1267, 2013
DOI:10.1016/j.bpj.2013.07.028
View abstract

Elsheshiny AA; Ashcroft AE; Harris SA A comparison of the electromechanical properties of structurally diverse proteins by molecular dynamics simulation. J Biomol Struct Dyn -, 2013
DOI:10.1080/07391102.2013.833864
View abstract

Jürgens MC; Ferguson N; Vörös J; Rautureau GJP; Shepherd DA; Ashcroft AE; Pye VE; Muldoon J; Johnson CM; Freund SMV The hepatitis B virus preS1 domain hijacks host trafficking proteins by motif mimicry Nature Chemical Biology 9 540-549, 2013
DOI:10.1038/nchembio.1294
View abstract

Fisher HC; Ashcroft AE; Smith M De novo sequencing of short interfering ribonucleic acids facilitated by use of tandem mass spectrometry with ion mobility spectrometry Rapid Communications in Mass Spectrometry 27 2247-2254, 2013
DOI:10.1002/rcm.6685
View abstract

Young LM; Cao P; Raleigh DP; Ashcroft AE; Radford SE Ion Mobility Spectrometry-Mass Spectrometry Defines the Oligomeric Intermediates in Amylin Amyloid Formation and the Mode of Action of Inhibitors. J Am Chem Soc 136 660-670, 2013
DOI:10.1021/ja406831n
View abstract

Lewis M; Arnot CJ; Gay NJ; Gangloff M; Beeston H; Ashcroft AE; McCoy A Cytokine Spätzle binds to the Drosophila immunoreceptor Toll with a neurotrophin-like specificity and couples receptor activation Proceedings of the National Academy of Sciences of the United States of America 110 20461-20466, 2013
DOI:10.1073/pnas.1317002110
View abstract

Ndlovu H; Ashcroft AE; Radford SE; Harris SA Effect of sequence variation on the mechanical response of amyloid fibrils probed by steered molecular dynamics simulation. Biophys J 102 587-596, 2012
DOI:10.1016/j.bpj.2011.12.047
View abstract

Hodkinson JP; Radford SE; Ashcroft AE Protein misfolding and toxicity in dialysis related amyloidosis In Non-fibrillar amyloidogenic protein assemblies– common cytotoxins underlying degenerative diseases , 2012
DOI:10.1007/978-94-007-2774-8_12

Morrissey B; Leney AC; Toste Rêgo A; Phan G; Allen WJ; Verger D; Waksman G; Ashcroft AE; Radford SE The role of chaperone-subunit usher domain interactions in the mechanism of bacterial pilus biogenesis revealed by ESI-MS. Mol Cell Proteomics 11 M111.015289-, 2012
DOI:10.1074/mcp.M111.015289
View abstract

Arscott S; Descatoire C; Buchaillot L; Ashcroft AE A snapshot of electrified nanodroplets undergoing Coulomb fission Applied Physics Letters 100 -, 2012
DOI:10.1063/1.3684979
View abstract

Kang L; Moriarty GM; Baum J; Woods LA; Ashcroft AE; Radford SE N-terminal acetylation ofα-synuclein induces increased transient helical propensity and decreased aggregation rates in the intrinsically disordered monomer Protein Science 21 911-917, 2012
DOI:10.1002/pro.2088
View abstract

Hodkinson JP; Radford SE; Ashcroft AE The role of conformational flexibility inβ -microglobulin amyloid fibril formation at neutral pH Rapid Communications in Mass Spectrometry 26 1783-1792, 2012
DOI:10.1002/rcm.6282
View abstract

Preston GW; Radford SE; Ashcroft AE; Wilson AJ Covalent cross-linking within supramolecular peptide structures. Anal Chem 84 6790-6797, 2012
DOI:10.1021/ac301198c
View abstract

Scarff CA; Sicorello A; Tomé RJL; Ashcroft AE; Radford SE; Macedo-Ribeiro S A tale of a tail: Structural insights into the conformational properties of the polyglutamine protein ataxin-3 International Journal of Mass Spectrometry 345-347 63-70, 2012
DOI:10.1016/j.ijms.2012.08.032
View abstract

Leney AC; McMorran LM; Radford SE; Ashcroft AE Amphipathic polymers enable the study of functional membrane proteins in the gas phase. Anal Chem 84 9841-9847, 2012
DOI:10.1021/ac302223s
View abstract

Phillips HIA; Chernikov AV; Fletcher NC; Ashcroft AE; Ault JR; Wilson AJ; Filby MH The use of electrospray mass spectrometry to determine speciation in a dynamic combinatorial library for anion recognition Chemistry - A European Journal 18 13733-13742, 2012
DOI:10.1002/chem.201201302
View abstract

Filby MH; Muldoon J; Dabb S; Fletcher NC; Ashcroft AE; Wilson AJ Protein surface recognition using geometrically pure Ru(II) tris(bipyridine) derivatives CHEM COMMUN 47 559-561, 2011
DOI:10.1039/c0cc04754f

Shepherd DA; Veesler D; Lichière J; Ashcroft AE; Cambillau C Unraveling lactococcal phage baseplate assembly by mass spectrometry. Mol Cell Proteomics 10 M111.009787-, 2011
DOI:10.1074/mcp.M111.009787
View abstract

Goulet A; Lai-Kee-Him J; Veesler D; Auzat I; Robin G; Shepherd DA; Ashcroft AE; Richard E; Lichiere J; Tavares P; Cambillau C; Bron P The Opening of the SPP1 Bacteriophage Tail, a Prevalent Mechanism in Gram-positive-infecting Siphophages J BIOL CHEM 286 25397-25405, 2011
DOI:10.1074/jbc.M111.243360

Leney AC; Phan G; Allen W; Verger D; Waksman G; Radford SE; Ashcroft AE Second Order Rate Constants of Donor-Strand Exchange Reveal Individual Amino Acid Residues Important in Determining the Subunit Specificity of Pilus Biogenesis J AM SOC MASS SPECTR 22 1214-1223, 2011
DOI:10.1007/s13361-011-0146-4

Woods LA; Platt GW; Hellewell AL; Hewitt EW; Homans SW; Ashcroft AE; Radford SE Ligand binding to distinct states diverts aggregation of an amyloid-forming protein NAT CHEM BIOL 7 730-739, 2011
DOI:10.1038/NCHEMBIO.635

Kaur-Atwal G; Reynolds JC; Mussell C; Champarnaud E; Knapman TW; Ashcroft AE; O'Connor G; Christie SDR; Creaser CS Determination of testosterone and epitestosterone glucuronides in urine by ultra performance liquid chromatography-ion mobility-mass spectrometry ANALYST 136 3911-3916, 2011
DOI:10.1039/c1an15450h

Smith DP; Woods LA; Radford SE; Ashcroft AE Structure and Dynamics of Oligomeric Intermediates in beta(2)-Microglobulin Self-Assembly BIOPHYS J 101 1238-1247, 2011
DOI:10.1016/j.bpj.2011.07.023

Pritchard C; Quaglia M; Ashcroft AE; O'Connor G Considering the advantages and pitfalls of the use of isotopically labeled protein standards for accurate protein quantification BIOANALYSIS 3 2797-2802, 2011
DOI:10.4155/BIO.11.281

Knapman TW; Berryman JT; Campuzano I; Harris SA; Ashcroft AE Considerations in experimental and theoretical collision cross-section measurements of small molecules using travelling wave ion mobility spectrometry-mass spectrometry INT J MASS SPECTROM 298 17-23, 2010
DOI:10.1016/j.ijms.2009.09.011

Smith DP; Radford SE; Ashcroft AE Elongated oligomers in beta(2)-microglobulin amyloid assembly revealed by ion mobility spectrometry-mass spectrometry P NATL ACAD SCI USA 107 6794-6798, 2010
DOI:10.1073/pnas.0913046107

Basnak G; Morton VL; Rolfsson O; Stonehouse NJ; Ashcroft AE; Stockley PG Viral Genomic Single-Stranded RNA Directs the Pathway Toward a T=3 Capsid J MOL BIOL 395 924-936, 2010
DOI:10.1016/j.jmb.2009.11.018

Muldoon J; Ashcroft AE; Wilson AJ Selective Protein-Surface Sensing Using Ruthenium(II) Tris(bipyridine) Complexes CHEM-EUR J 16 100-103, 2010
DOI:10.1002/chem.200902368

Jones LN; Baldwin SA; Henderson PJF; Ashcroft AE Defining topological features of membrane proteins by nanoelectrospray ionisation mass spectrometry RAPID COMMUN MASS SP 24 276-284, 2010
DOI:10.1002/rcm.4387

Ashcroft AE Mass Spectrometry and the Amyloid Problem-How Far Can We Go in the Gas Phase? J AM SOC MASS SPECTR 21 1087-1096, 2010
DOI:10.1016/j.jasms.2010.02.026

Knapman TW; Morton VL; Stonehouse NJ; Stockley PG; Ashcroft AE Determining the topology of virus assembly intermediates using ion mobility spectrometry-mass spectrometry RAPID COMMUN MASS SP 24 3033-3042, 2010
DOI:10.1002/rcm.4732

Morton VL; Burkitt W; O'Connor G; Stonehouse NJ; Stockley PG; Ashcroft AE RNA-induced conformational changes in a viral coat protein studied by hydrogen/deuterium exchange mass spectrometry PHYS CHEM CHEM PHYS 12 13468-13475, 2010
DOI:10.1039/c0cp00817f

Morton VL; Dykeman EC; Stonehouse NJ; Ashcroft AE; Twarock R; Stockley PG The Impact of Viral RNA on Assembly Pathway Selection J MOL BIOL 401 298-308, 2010
DOI:10.1016/j.jmb.2010.05.059

Smith DP; Ashcroft AE; Radford SE Hemodialysis-Related Amyloidosis Protein Misfolding Diseases: Current and Emerging Principles and Therapies 347-379, 2010
DOI:10.1002/9780470572702.ch16

Smith DP; Knapman TW; Campuzano I; Malham RW; Berryman JT; Radford SE; Ashcroft AE Deciphering drift time measurements from travelling wave ion mobility spectrometry-mass spectrometry studies EUR J MASS SPECTROM 15 113-130, 2009
DOI:10.1255/ejms.947

Hodkinson JP; Jahn TR; Radford SE; Ashcroft AE HDX-ESI-MS Reveals Enhanced Conformational Dynamics of the Amyloidogenic Protein beta(2)-Microglobulin upon Release from the MHC-1 J AM SOC MASS SPECTR 20 278-286, 2009
DOI:10.1016/j.jasms.2008.10.005

Fadouloglou VE; Bastaki MN; Ashcroft AE; Phillips SEV; Panopoulos NJ; Glykos NM; Kokkinidis M On the quaternary association of the type III secretion system HrcQ(B)-C protein: Experimental evidence differentiates among the various oligomerization models J STRUCT BIOL 166 214-225, 2009
DOI:10.1016/j.jsb.2009.01.008

Taylor DR; Parkin ET; Cocklin SL; Ault JR; Ashcroft AE; Turner AJ; Hooper NM Role of ADAMs in the Ectodomain Shedding and Conformational Conversion of the Prion Protein J BIOL CHEM 284 22590-22600, 2009
DOI:10.1074/jbc.M109.032599

Rolfsson O; Toropova K; Morton V; Francese S; Basnak G; Thompson GS; Homans SW; Ashcroft AE; Stonehouse NJ; Ranson NA; Stockley PG RNA packing specificity and folding during assembly of the bacteriophage MS2 COMPUT MATH METHOD M 9 339-349, 2008
DOI:10.1080/17486700802168445

Rose RJ; Verger D; Daviter T; Remaut H; Paci E; Waksman G; Ashcroft AE; Radford SE Unraveling the molecular basis of subunit specificity in P pilus assembly by mass spectrometry P NATL ACAD SCI USA 105 12873-12878, 2008
DOI:10.1073/pnas.0802177105

Morton VL; Stockley PG; Stonehouse NJ; Ashcroft AE INSIGHTS INTO VIRUS CAPSID ASSEMBLY FROM NON-COVALENT MASS SPECTROMETRY MASS SPECTROM REV 27 575-595, 2008
DOI:10.1002/mas.20176

Verger D; Rose RJ; Paci E; Costakes G; Daviter T; Hultgren S; Remaut H; Ashcroft AE; Radford SE; Waksman G Structural Determinants of Polymerization Reactivity of the P pilus Adaptor Subunit PapF STRUCTURE 16 1724-1731, 2008
DOI:10.1016/j.str.2008.08.012

Smith DP; Anderson J; Plante J; Ashcroft AE; Radford SE; Wilson AJ; Parker MJ Trifluoromethyldiazirine: an effective photo-induced cross-linking probe for exploring amyloid formation CHEM COMMUN 5728-5730, 2008
DOI:10.1039/b813504e

Knapman TW; Aggeli A; Ashcroft AE Critical concentrations of beta-sheet peptide self-assembly quantified directly by nanoelectrospray ionization mass spectrometry RAPID COMMUN MASS SP 22 1611-1614, 2008
DOI:10.1002/rcm.3540

Rose RJ; Welsh TS; Waksman G; Ashcroft AE; Radford SE; Paci E Donor-strand exchange in chaperone-assisted pilus assembly revealed in atomic detail by molecular dynamics J MOL BIOL 375 908-919, 2008
DOI:10.1016/j.jmb.2007.10.077

Stockley PG; Rolfsson O; Thompson GS; Basnak G; Francese S; Stonehouse NJ; Homans SW; Ashcroft AE A simple, RNA-mediated allosteric switch controls the pathway to formation of a T=3 viral capsid J MOL BIOL 369 541-552, 2007
DOI:10.1016/j.jmb.2007.03.020

Legrand B; Ashcroft AE; Buchaillot L; Arscott S SOI-based nanoelectrospray emitter tips for mass spectrometry: a coupled MEMS and microfluidic design J MICROMECH MICROENG 17 509-514, 2007
DOI:10.1088/0960-1317/17/3/013

Arscott S; Legrand B; Buchaillot L; Ashcroft AE A silicon beam-based microcantilever nanoelectrosprayer SENSOR ACTUAT B-CHEM 125 72-78, 2007
DOI:10.1016/j.snb.2007.01.040

White HD; Ashcroft AE Real-time measurement of myosin-nucleotide noncovalent complexes by electrospray ionization mass spectrometry BIOPHYS J 93 914-919, 2007
DOI:10.1529/biophysj.106.101618

Smith DP; Giles K; Bateman RH; Radford SE; Ashcroft AE Monitoring copopulated conformational states during protein folding events using Electrospray ionization-ion mobility spectrometry-mass spectrometry J AM SOC MASS SPECTR 18 2180-2190, 2007
DOI:10.1016/j.jasms.2007.09.017

Ironmonger A; Whittaker B; Baron AJ; Clique B; Adams CJ; Ashcroft AE; Stockley PG; Nelson A Scanning conformational space with a library of stereo- and regiochemically diverse aminoglycoside derivatives: the discovery of new ligands for RNA hairpin sequences ORG BIOMOL CHEM 5 1081-1086, 2007
DOI:10.1039/b618683a

Legrand B; Buchaillot L; Arscott S; Ashcroft AE Silicon nano-ESI emitters for mass spectrometry: A mixed micromechanical and microfluidic design TRANSDUCERS and EUROSENSORS '07 - 4th International Conference on Solid-State Sensors, Actuators and Microsystems 791-794, 2007
DOI:10.1109/SENSOR.2007.4300249
View abstract

Remaut H; Rose RJ; Hannan TJ; Hultgren SJ; Radford SE; Ashcroft AE; Waksman G Donor-strand exchange in chaperone-assisted pilus assembly proceeds through a concerted beta strand displacement mechanism MOL CELL 22 831-842, 2006
DOI:10.1016/j.molcel.2006.05.033

Arscott S; Gaudet M; Brinkmann M; Ashcroft AE; Blossey R Capillary filling of miniaturized sources for electrospray mass spectrometry J PHYS-CONDENS MAT 18 S677-S690, 2006
DOI:10.1088/0953-8984/18/18/S13

Smith AM; Jahn TR; Ashcroft AE; Radford SE Direct observation of oligomeric species formed in the early stages of amyloid fibril formation using electrospray ionisation mass spectrometry J MOL BIOL 364 9-19, 2006
DOI:10.1016/j.jmb.2006.08.081

Khan A; Ashcroft AE; Higenell V; Korchazhkina OV; Exley C Metals accelerate the formation and direct the structure of amyloid fibrils of NAC JOURNAL OF INORGANIC BIOCHEMISTRY 99 1920-1927, 2005
DOI:10.1016/j.jinorgbio.2005.06.018

Stockley PG; Ashcroft AE; Francese S; Thompson GS; Ranson NA; Smith AM; Homans SW; Stonehouse NJ Dissecting the fine details of assembly of a T=3 capsid Journal of Theoretical Medicine 6 119-125, 2005
DOI:10.1080/10273660500149869

Ashcroft AE; Lago H; Macedo JMB; Horn WT; Stonehouse NJ; Stockley PG Engineering thermal stability in RNA phage capsids via disulphide bonds J NANOSCI NANOTECHNO 5 2034-2041, 2005
DOI:10.1166/jnn.2005.507

Ashcroft AE Recent developments in electrospray ionisation mass spectrometry: noncovalently bound protein complexes NAT PROD REP 22 452-464, 2005
DOI:10.1039/b417724j

Descatoire C; Troadec D; Buchaillot L; Arscott S; Ashcroft A A nanofluidic electrospray source fabricated using focused ion beam etching Micro Total Analysis Systems - Proceedings of MicroTAS 2005 Conference: 9th International Conference on Miniaturized Systems for Chemistry and Life Sciences 1 241-243, 2005
View abstract

Khan A; Ashcroft AE; Korchazhkina OV; Exley C Metal-mediated formation of fibrillar ABri amyloid J INORG BIOCHEM 98 2006-2010, 2004
DOI:10.1016/j.jinorgbio.2004.09.004

Ashcroft AE; Brenton AG; Monaghan JJ Advances in Mass Spectrometry 16: Proceedings of the 16th International Mass Spectrometry Conference, 2004

Rodgers-Gray TP; Smith JE; Ashcroft AE; Isaac RE; Dunn AM Mechanisms of parasite-induced sex reversal in Gammarus duebeni INT J PARASITOL 34 747-753, 2004
DOI:10.1016/j.ijpara.2004.01.005

Borysik AJH; Radford SE; Ashcroft AE Co-populated conformational ensembles of beta(2)-microglobulin uncovered quantitatively by electrospray ionization mass spectrometry J BIOL CHEM 279 27069-27077, 2004
DOI:10.1074/jbc.M401472200

Borysik AJH; Read P; Little DR; Bateman RH; Radford SE; Ashcroft AE Separation of beta(2)-microglobulin conformers by high-field asymmetric waveform ion mobility spectrometry (FAIMS) coupled to electrospray ionisation mass spectrometry RAPID COMMUN MASS SP 18 2229-2234, 2004
DOI:10.1002/rcm.1613

Pearson AR; Jones LH; Higgins LA; Ashcroft AE; Wilmot CM; Davidson VL Understanding quinone cofactor biogenesis in methylamine dehydrogenase through novel cofactor generation BIOCHEMISTRY-US 42 3224-3230, 2003
DOI:10.1021/bi027073a

Korchazhkina OV; Ashcroft AE; Croom J; Exley C Does either the gastrointestinal peptide PYY or the neuropeptide NPY bind aluminium? J INORG BIOCHEM 94 372-380, 2003
DOI:10.1016/S0162-0134(03)00031-X

Ashcroft AE Protein and peptide identification: the role of mass spectrometry in proteomics NAT PROD REP 20 202-215, 2003
DOI:10.1039/b201368c

Meky FA; Turner PC; Ashcroft AE; Miller JD; Qiao YL; Roth MJ; Wild CP Development of a urinary biomarker of human exposure to deoxynivalenol FOOD CHEM TOXICOL 41 265-273, 2003

Bailey S; Sedelnikova SE; Mesa P; Ayora S; Waltho JP; Ashcroft AE; Baron AJ; Alonso JC; Rafferty JB Structural analysis of Bacillus subtilis SPP1 phage helicase loader protein G39P J BIOL CHEM 278 15304-15312, 2003
DOI:10.1074/jbc.M209300200

Korchazhkina OV; Ashcroft AE; Kiss T; Exley C The degradation of A-beta 25-35 by the serine protease plasmin is inhibited by aluminium Journal of Alzheimer's Disease 4 357-367, 2002
View abstract

Goodall JJ; Booth VK; Ashcroft AE; Wharton CW Hydrogen-bonding in 2-aminobenzoyl-alpha-chymotrypsin formed by acylation of the enzyme with isatoic anhydride: IR and mass spectroscopic studies CHEMBIOCHEM 3 68-75, 2002

Ashcroft AE; Brinker A; Coyle JE; Weber F; Kaiser M; Moroder L; Parsons MR; Jager J; Hartl UF; Hayer-Hartl M; Radford SE Structural plasticity and noncovalent substrate binding in the GroEL apical domain - A study using electrospray ionization mass spectrometry and fluorescence binding studies J BIOL CHEM 277 33115-33126, 2002
DOI:10.1074/jbc.M203398200

Ashcroft AE; Venter H; Keen JN; Henderson PJF; Herbert RB Molecular dissection of membrane transport proteins: mass spectrometry and sequence determination of the galactose-H+symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-13C]histidine.. Biochemical Journal 363 243-252, 2002
DOI:10.1042/0264-6021:3630243

Standeven KF; Ariens RAS; Whitaker P; Ashcroft AE; Weisel JW; Grant PJ The effect of dimethylbiguanide on thrombin activity, FXIII activation, fibrin polymerization, and fibrin clot formation DIABETES 51 189-197, 2002

Treumann A; Feng XD; McDonnell L; Derrick PJ; Ashcroft AE; Chatterjee D; Homans SW 5-methylthiopentose: a new substituent on lipoarabinomannan in Mycobacterium tuberculosis J MOL BIOL 316 89-100, 2002
DOI:10.1006/jmbi.2001.5317

Ramoni R; Vincent F; Ashcroft AE; Accornero P; Grolli S; Valencia C; Tegoni M; Cambillau C Control of domain swapping in bovine odorant-binding protein BIOCHEM J 365 739-748, 2002

Ashcroft AE; Grant PJ; Ariens RAS A study of human coagulation factor XIII A-subunit by electrospray ionisation mass spectrometry RAPID COMMUN MASS SP 14 1607-1611, 2000

Jones S; Reader JS; Healy M; Capaldi AP; Ashcroft AE; Kalverda AP; Smith DA; Radford SE Partially unfolded species populated during equilibrium denaturation of the beta-sheet protein Y74W apo-pseudoazurin BIOCHEMISTRY-US 39 5672-5682, 2000

Parrott AM; Lago H; Adams CJ; Ashcroft AE; Stonehouse NJ; Stockley PG RNA aptamers for the MS2 bacteriophage coat protein and the wild-type RNA operator have similar solution behaviour NUCLEIC ACIDS RES 28 489-497, 2000

Grant PJ; Ashcroft AE; Ariens R; Standeven K; Whitaker P Biguanides inhibit FXIII induced cross-linking activity and FXIII activation peptide release by thrombin Fibrinolysis and Proteolysis 14 47-47, 2000
View abstract

Kulkarni SK; Ashcroft AE; Carey M; Masselos D; Robinson CV; Radford SE A near-native state on the slow refolding pathway of hen lysozyme PROTEIN SCI 8 35-44, 1999

Coyle JE; Texter FL; Ashcroft AE; Masselos D; Robinson CV; Radford SE GroEL accelerates the refolding of hen lysozyme without changing its folding mechanism NAT STRUCT BIOL 6 683-690, 1999

Keen JN; Ashcroft AE Sequence analysis of expressed proteins In Post-Translational Processing - A Practical Approach , 1999

Whitaker P; Ariens RAS; Ashcroft AE; Grant PJ Metformin reduces FXIII antigen and activity levels in type II diabetes, and inhibits FXIII cross-linking activity in vitro THROMB HAEMOSTASIS 12-12, 1999

Ariens RAS; Ashcroft AE; Grant PJ Purification and mass spectrometric analysis of human coagulation factor XIII THROMB HAEMOSTASIS 80-80, 1999

Thomson GJ; Howlett GJ; Ashcroft AE; Berry A The dhnA gene of Escherichia coli encodes a Class I fructose bisphosphate aldolase BIOCHEM J 331 437-445, 1998

Vanhoutte K; Van Dongen W; Esmans E; Van Soest REJ; Chervet JP; Jarvis SA; Ashcroft AE; Hudson AJ A nanoflow electrospray method for DNA-adduct analysis American Biotechnology Laboratory 16 41-43, 1998
View abstract

Ashcroft AE Ionization Methods in Organic Mass Spectrometry, 1997

Daley DJ; Scammell RD; James D; Monks I; Raso R; Ashcroft AE High Throughput LC/MS Verification of Drug Candidates from 96-well plates American Biotechnology Laboratory 15 pp.24-, 1997

Ashcroft AE; Major HJ; Wilson ID; Nicholls A; Nicholson JK Application of capillary electrophoresis mass spectrometry to the analysis of urine samples from animals and man containing paracetamol and phenacetin and their metabolites ANAL COMMUN 34 41-43, 1997

Claude C; Langridge J; Bordoli RS; Hoyes J; Ashcroft AE Protein Sequencing by Quadrupole -Time of Flight Coupling Biofutur 164 pp.11-, 1997

Claude C; Langridge J; Bordoli B; Hoyes J; Ashcroft A Spectrometrie de masse: Sequencage de proteines par couplage quadripole-temps de vol Biofutur 11-12, 1997

Major HJ; Ashcroft AE Analysis of a standard peptide mixture by capillary electrophoresis with mass spectrometry and with tandem mass spectrometry. Rapid Commun Mass Spectrom 10 1421-1426, 1996
DOI:10.1002/(SICI)1097-0231(199608)10:11<1421::AID-RCM651>3.0.CO;2-D
View abstract

Scullion SD; Clench MR; Cooke M; Ashcroft AE Determination of surfactants in surface water by solid-phase extraction, liquid chromatography and liquid chromatography-mass spectrometry J CHROMATOGR A 733 207-216, 1996

ASHCROFT AE; MAJOR HJ; LOWES S; WILSON ID IDENTIFICATION OF NONSTEROIDAL ANTIINFLAMMATORY DRUGS AND THEIR METABOLITES IN SOLID-PHASE EXTRACTS OF HUMAN URINE USING CAPILLARY ELECTROPHORESIS-MASS SPECTROMETRY ANAL PROC 32 459-462, 1995

PETERKATALINIC J; ASHCROFT A; GREEN B; HANISCH FG; NAKAHARA Y; ILIJIMA H; OGAWA T POTENTIAL OF ELECTROSPRAY MASS-SPECTROMETRY FOR STRUCTURAL STUDIES OF O-GLYCOAMINO-ACIDS AND O-GLYCOAMINO-PEPTIDES WITH MULTIPLE ALPHA-2,6-SIALOSYL-TN GLYCOSYLATION ORG MASS SPECTROM 29 747-752, 1994

ASHTON DS; ASHCROFT AE; BEDDELL CR; COOPER DJ; GREEN BN; OLIVER RWA ON THE ANALYSIS OF BOVINE TRYPSIN BY ELECTROSPRAY MASS-SPECTROMETRY BIOCHEM BIOPH RES CO 199 694-698, 1994

HASKINS NJ; ASHCROFT AE; PHILLIPS A; HARRISON M THE EVALUATION OF SEVERAL ELECTROSPRAY SYSTEMS AND THEIR USE IN NONCOVALENT BONDING STUDIES RAPID COMMUN MASS SP 8 120-125, 1994

PETERKATALINIC J; WILLIGER K; EGGE H; GREEN B; ASHCROFT A; HANISCH FG; SCHINDLER D O-GLYCOPEPTIDES FROM URINE OF PATIENTS WITH ALPHA-N-ACETYLGALACTOSAMINIDASE DEFICIENCY STUDIED BY ELECTROSPRAY (ES) AND FAST-ATOM-BOMBARDMENT (FAB) MASS-SPECTROMETRY GLYCOCONJUGATE J 10 280-280, 1993

TARELLI E; ASHCROFT AE; CALAM DH; CORRAN PH; GREEN BN; WHEELER SF HUMAN ALPHA-FETOPROTEIN - MOLECULAR-WEIGHT DATA FROM ELECTROSPRAY MASS-SPECTROMETRY (ESMS) ANALYSIS BIOL MASS SPECTROM 21 315-317, 1992

Vose CW; Whelpton R; Ashcroft AE; Everett JR; Nicholson JK; Wilson ID; Hutt AJ; Morgan ED; Huang H Biomedical and pharmaceutical chemistry Analytical Proceedings 28 177-188, 1991
DOI:10.1039/AP9912800177

GRIFFITHS EC; KELLY JA; ASHCROFT A; WARD DJ; ROBSON B COMPARATIVE METABOLISM AND CONFORMATION OF TRH AND ITS ANALOGS ANN NY ACAD SCI 553 217-231, 1989

ASHCROFT AE MICRO AND MICROBORE LIQUID-CHROMATOGRAPHY FAST ATOM BOMBARDMENT-MASS SPECTROMETRY (LC-FAB-MS) ANALYSIS OF PEPTIDES AND ANTIBIOTICS ORG MASS SPECTROM 22 754-757, 1987

BARBER M; TETLER LW; BELL D; ASHCROFT AE; BROWN RS; MOORE C APPLICATIONS OF A CONTINUOUS-FLOW PROBE IN FAB MASS-SPECTROMETRY ORG MASS SPECTROM 22 647-650, 1987

ASHCROFT AE; CHAPMAN JR; COTTRELL JS CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY J CHROMATOGR 394 15-20, 1987

HUTCHINSON DW; WOOLFITT AR; ASHCROFT AE ANALYSIS OF PEPTIDE DIGESTS USING HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY COUPLED WITH CONTINUOUS-FLOW FAST-ATOM-BOMBARDMENT MASS-SPECTROMETRY (FAB-MS) ORG MASS SPECTROM 22 304-306, 1987

ASHCROFT AE; CHAPMAN JR; KEARNS M; ALMOND MF; PRATT JAE A NEW INSTRUMENT FOR GC LC-MS AM LAB 17 59-&, 1985

ASHCROFT AE; DAVIES DT; SUTHERLAND JK THE SYNTHESIS OF ANTHRACYCLINONES .2. THE SYNTHESIS OF 7,9-BISDEOXYCARMINOMYCINONE AND 7,9-BISDEOXYCARDAUNOMYCINONE TETRAHEDRON 40 4579-4583, 1984

OPPOLZER W; BEGLEY T; ASHCROFT A APPLICATION OF THE INTRAMOLECULAR MAGNESIUM-ENE REACTION TO THE STEREOCONTROLLED TOTAL SYNTHESES OF (+/-)-12-ACETOXYSINULARENE AND (+/-)-2-EPI-12-ACETOXYSINULARENE TETRAHEDRON LETT 25 825-828, 1984

Ashcroft AE; Kearns M; Greathead RJ; Almond M; Compson KR COMPARISON OF TECHNIQUES FOR THE ANALYSIS OF ETHYLENE DIBROMIDE. Annual Conference on Mass Spectrometry and Allied Topics 297-298, 1984
View abstract

ASHCROFT AE; SUTHERLAND JK A REGIOSELECTIVE SYNTHESIS OF 8,10-DIDEOXYCARMINOMYCINONE J CHEM SOC CHEM COMM 1075-1076, 1981

Smith DP; Ashcroft AE; Radford SE Protein mis-folding diseases: current and emerging principles and possible therapies– haemodialysis related amyloidosis In Protein Misfolding Diseases: Current and Emerging Principles and Therapies ,

Hodkinson JP; Ashcroft AE; Radford SE Protein misfolding and toxicity in dialysis related amyloidosis In Prefibrillar amyloidogenic protein assemblies– common cytotoxins underlying degenerative diseases ,