Faculty of Biological Sciences

Prof Michael McPherson

BSc, 1979; PhD 1983; Leeds.
Interim Dean & Pro-Dean for Student Education, Professor of Biomolecular Engineering, Director of the BioScreening Technology Group
School of Molecular and Cellular Biology

Background: Lecturer in Biotechnology 1985, Senior Lecturer 1992, Reader 1998; Leverhulme Trust/Royal Society Senior Research Fellow 1993-4; Professor 2002.

Contact:  Miall 7.07 (PA, Dean/Sue Yeadon, Pro-Dean/Christine Everson) | +44(0) 113 34 32595/35647/32437 | email address for  

You can read more about Prof McPherson's interests here:
http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=MJM

Research Interests

Protein engineering, Adhirons (artificial binding proteins) and synthetic biology

Adhiron binding proteins as research tools, for diagnostics, imaging, therapy and synthetic biology

We have developed a small stable protein scaffold called AdhironTM that is used to create complex libraries with randomized regions that create novel binding sites. The adhirons are binding proteins that represent small, robust alternatives to antibodies.

By using phage display the adhiron libraries can be screened against a wide range of  target molecules to identify specific binders that can then be readily produced on a large scale. Adhirons represent important tools for scientific investigations, essentially replacing antibodies with stable simple reagents.

They can be used in applications such as biosensors, development of imaging reagents, as co-crystallization chaperones, to identify sites that modulate or interfere with function thus identifying druggable sites, and as therapeutic agents. For example, we are interested in the generation of binding partners to disease related biomarkers for incorporation into multiplex individually addressable electronic detection devices, or as visual sensors by coupling to quantum dots for clinical applications.

However, Adhirons can be selected for use in any diagnostic application including those for environmental, security or agricultural purposes. We are also interested in large scale screens such as membrane protein structural proteomics through the production of co-crystallization chaperones. Other applications relate to in vivo expression to interfere with protein-protein interactions including the ability to modulate protein function as well as to provide scaffolds for applications including synthetic biology for the development of organisms that can undertake new and important functions.

We have recently established a Bioscreening Technology Group supported by the Leeds Biomedical Health Research Centre to provide a screening service for academics and clinicians and we undertake a range of collaborative projects with colleagues in Leeds, nationally and internationally.

Copper enzymes that build their own cofactors

We study copper oxidases that generate their own cofactors by post-translational modification of active site amino-acids. We use gene cloning, protein engineering, protein expression, enzymology, spectroscopy and X-ray crystallography to study the catalytic mechanisms, substrate binding and cofactor formation in these enzymes. We also engineer the enzymes for novel functionality using saturation mutagenesis and directed evolution methods. We are interested in structure-function studies on a range of these proteins including those associated with human health and disease. Particular targets include galactose oxidase, amine oxidases and lysyl oxidase.

Find out more about research affiliations at:

 

Faculty Research and Innovation


Studentship information

Undergraduate project topics:

  • Projects are available as either laboratory or literature format in the areas indicated in the Research Interests section below.

Postgraduate studentship areas:

  • Protein engineering and directed evolution of copper oxidases. This involves a range of molecular biology and protein characterisation methods with the possibility of x-ray structure studies of proteins.
  • Adhirons as artificial binding proteins for diagnostics, imaging and therapeutics and synthetic biology applications. This may involve design and construction of new libraries, the use of phage display libraries for screening against targets, validation of hits, protein expression and characterization. The projects are highly collaborative with other biological and physical scientists, clinicians and engineers. Targets can be soluble and membrane proteins, peptides, cells, small molecules etc.
  • Projects are available in all areas of research, broadly defined as above.

See also:

Admin roles

Biomedical Health Research Centre Executive Committee
Biomedical Health Research Centre Steering Group
Committee on Applications
Curriculum Enhancement Project Board
External Examiner (taught course): Liverpool
Faculty Research & Innovation Committee
FBS Student Recruitment Committee, Chair
FBS Employability Committee (Chair)
FBS Equality and Diversity Committee
FBS Exective Committee
FBS Facilities Liaison Group
FBS Faculty Board
FBS FTSEC (Chair)
FBS GTSEC
FBS Industrial Advisory Board, Academic Chair
FBS Reward and Recognition Committee
FBS Strategy Group
FBS UGTSEC
FBS Undergraduate Recruitment Group
Graduate Board, Taught Student Board
Honorary Degrees Committee
Library Strategy Committee
Pricing and Scholarship Sub Group
Pro-Dean for Student Education
PVC and Pro-Deans meetings
Student Recruitment Committee
Taught Student Education Board
Teaching Enhancement Scheme group, Chair
University N8 Industrial Biotechnology representative
University Research Ethics Committee

Modules taught

BIOC2301/BIOL2111 - Integrated Biochemistry/Genetic Engineering
BIOC3160 - Lab/Lit/Comp Research Project
BIOC3231/32/33 a - ATU 10 - Directed evolution
BIOC3231/32/33 b - ATU 33 - Synthetic Biology
BIOL1302 - Intro Skills - Biological Sci
BIOL3306 - Biol Sciences Research Project
BIOL5271M - Research Planning and Scientific Communication
BIOL5272M - Advanced Biomolecular Technologies
BIOL5292M - Bioscience MSc Research Proj
BIOL5294M - MSc Bioscience Res Project Pro
BIOW5901X - Foundation module

Committees

Chair of Faculty Taught Student Education Committee
Member of Graduate School Taught Student Education Committee
Member of Undergraduate School Taught Student Education Committee

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Prof Michael McPherson  (Interim Dean & Pro-Dean for Student Education, Professor of Biomolecular Engineering, Director of the BioScreening Technology Group)

Protein engineering, Adhirons (artificial binding proteins) and synthetic biology 


Dr Sarah Deacon  (Research Fellow)

Development of Antibody Mimetics For Clinical Diagnostics 

Dr Thembaninkosi Gaule  (Research Fellow)

Dr Suja George  (Research Fellow)

Miss Anna Tang  (Research Technician)

Dr Christian Tiede  (Research Fellow)


Postgraduates

Modupe Ajayi  
James Edmundson  
Birthe Lang  
Thanisorn Mahatnirunkul  
Philip Morrison  
Janice Robottom  
Mark Sellick  
Pattanapong Thangsunan  

Tiede C; Tang AA; Deacon SE; Mandal U; Nettleship JE; Owen RL; George SE; Harrison DJ; Owens RJ; Tomlinson DC; McPherson MJ Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel 27 145-155, 2014
DOI:10.1093/protein/gzu007
View abstract

Yuzugullu Y; Trinh CH; Fairhurst L; McPherson MJ; Pearson AR; Ogel ZB Investigating the active centre of the Scytalidium thermophilum catalase Acta Crystallographica Section F: Structural Biology and Crystallization Communications 69 369-375, 2013
DOI:10.1107/S1744309113004211
View abstract

Kyle S; James KAR; McPherson MJ Recombinant production of the therapeutic peptide lunasin MICROBIAL CELL FACTORIES 11 -, 2012
DOI:10.1186/1475-2859-11-28

Huysmans GHM; Chan N; Baldwin JM; Postis VLG; Tzokov SB; Deacon SE; Yao SYM; Young JD; McPherson MJ; Bullough PA; Baldwin SA A urea channel from Bacillus cereus reveals a novel hexameric structure BIOCHEMICAL JOURNAL 445 157-166, 2012
DOI:10.1042/BJ20120169

Kyle S; Felton SH; McPherson MJ; Aggeli A; Ingham E Rational molecular design of complementary self-assembling peptide hydrogels. Adv Healthc Mater 1 640-645, 2012
DOI:10.1002/adhm.201200047
View abstract

Prakash A; Parsons SJ; Kyle S; McPherson MJ Recombinant production of self-assemblingβ-structured peptides using SUMO as a fusion partner. Microb Cell Fact 11 92-, 2012
DOI:10.1186/1475-2859-11-92
View abstract

Smith MA; Knowles PF; McPherson MJ; Pearson AR Dissecting the mechanism of oxygen trafficking in a metalloenzyme FARADAY DISCUSS 148 269-282, 2011
DOI:10.1039/c005054g

Deacon SE; McPherson MJ Enhanced Expression and Purification of Fungal Galactose Oxidase in Escherichia coli and Use for Analysis of a Saturation Mutagenesis Library CHEMBIOCHEM 12 593-601, 2011
DOI:10.1002/cbic.201000634

Newstead S; Drew D; Cameron AD; Postis VLG; Xia XB; Fowler PW; Ingram JC; Carpenter EP; Sansom MSP; McPherson MJ; Baldwin SA; Iwata S Crystal structure of a prokaryotic homologue of the mammalian oligopeptide-proton symporters, PepT1 and PepT2 EMBO J 30 417-426, 2011
DOI:10.1038/emboj.2010.309

Kurtis CRP; Knowles PF; Parsons MR; Gaule TG; Phillips SEV; McPherson MJ Tyrosine 381 in E. coli copper amine oxidase influences substrate specificity J NEURAL TRANSM 118 1043-1053, 2011
DOI:10.1007/s00702-011-0620-y

Troshin PV; Barton GJ; Postis VL; Baldwin SA; Ashworth D; McPherson MJ PIMS sequencing extension: A laboratory information management system for DNA sequencing facilities BMC Research Notes 4 -, 2011
DOI:10.1186/1756-0500-4-48
View abstract

Kyle S; McPherson MJ; Ingham E; Aggeli A Complementary self-assembling peptides: Effect of molecular design on biological properties European Cells and Materials 22 60-60, 2011

Smith MA; Pirrat P; Pearson AR; Kurtis CRP; Trinh CH; Gaule TG; Knowles PF; Phillips SEV; McPherson MJ Exploring the Roles of the Metal Ions in Escherichia coli Copper Amine Oxidase BIOCHEMISTRY-US 49 1268-1280, 2010
DOI:10.1021/bi901738k

Charlton WL; Harel HYM; Bakhetia M; Hibbard JK; Atkinson HJ; McPherson MJ Additive effects of plant expressed double-stranded RNAs on root-knot nematode development INT J PARASITOL 40 855-864, 2010
DOI:10.1016/j.ijpara.2010.01.003

Kyle S; Aggeli A; Ingham E; McPherson MJ Recombinant self-assembling peptides as biomaterials for tissue engineering BIOMATERIALS 31 9395-9405, 2010
DOI:10.1016/j.biomaterials.2010.08.051

Kocabas DS; Pearson AR; Phillips SEV; Bakir U; Ogel ZB; McPherson MJ; Trinh CH Crystallization and preliminary X-ray analysis of a bifunctional catalase-phenol oxidase from Scytalidium thermophilum ACTA CRYSTALLOGR F 65 486-488, 2009
DOI:10.1107/S1744309109012007

Kyle S; Aggeli A; Ingham E; McPherson MJ Production of self-assembling biomaterials for tissue engineering TRENDS BIOTECHNOL 27 423-433, 2009
DOI:10.1016/j.tibtech.2009.04.002

Riley JM; Aggeli A; Koopmans RJ; McPherson MJ Bioproduction and Characterization of a pH Responsive Self-Assembling Peptide BIOTECHNOL BIOENG 103 241-251, 2009
DOI:10.1002/bit.22274

Kocabas DS; Bakir U; Phillips SE; Mcpherson MJ; Ogel ZB Purification, characterization and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum NEW BIOTECHNOL 25 S92-S93, 2009
DOI:10.1016/j.nbt.2009.06.370

McPherson MJ; James K; Kyle S; Parsons S; Riley J Recombinant production of self-assembling peptides In Engineering aspects of self-organising materials , 2009

McPherson MJ; James K; Kyle S; Parsons S; Riley J Recombinant Production of Self-Assembling Peptides Advances in Chemical Engineering 35 79-117, 2009
DOI:10.1016/S0065-2377(08)00204-4
View abstract

Postis VLG; Deacon SE; Roach PCJ; Wright GSA; Xia X; Ingram JC; Hadden JM; Henderson PJF; Phillips SEV; McPherson MJ; Baldwin SA A high-throughput assay of membrane protein stability MOL MEMBR BIOL 25 617-624, 2008
DOI:10.1080/09687680802530469

Rahman M; Patching SG; Ismat F; Henderson PJF; Herbert RB; Baldwin SA; McPherson MJ Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR MOL MEMBR BIOL 25 683-690, 2008
DOI:10.1080/09687680802495267

Kocabas DS; Bakir U; Phillips SEV; McPherson MJ; Ogel ZB Purification, characterization, and identification of a novel bifunctional catalase-phenol oxidase from Scytalidium thermophilum APPL MICROBIOL BIOT 79 407-415, 2008
DOI:10.1007/s00253-008-1437-y

Rogers MS; Hurtado-Guerrero R; Firbank SJ; Halcrow MA; Dooley DM; Phillips SEV; Knowles PF; McPherson MJ Cross-link formation of the cysteine 228-tyrosine 272 catalytic cofactor of galactose oxidase does not require dioxygen BIOCHEMISTRY-US 47 10428-10439, 2008
DOI:10.1021/bi8010835

Xia XB; Postis VLG; Rahman M; Wright GSA; Roach PCJ; Deacon SE; Ingram JC; Henderson PJF; Findlay JBC; Phillips SEV; McPherson MJ; Baldwin SA Investigation of the structure and function of a Shewanella oneidensis arsenical-resistance family transporter MOL MEMBR BIOL 25 691-705, 2008
DOI:10.1080/09687680802535930

Pirrat P; Smith MA; Pearson AR; McPherson MJ; Phillips SEV Structure of a xenon derivative of Escherichia coli copper amine oxidase: confirmation of the proposed oxygen-entry pathway ACTA CRYSTALLOGR F 64 1105-1109, 2008
DOI:10.1107/S1744309108036373

Deacon SE; Roach PCJ; Postis VLG; Wright GSA; Xia XB; Phillips SEV; Knox JP; Henderson PJF; McPherson MJ; Baldwin SA Reliable scale-up of membrane protein over-expression by bacterial auto-induction: From microwell plates to pilot scale fermentations MOL MEMBR BIOL 25 588-598, 2008
DOI:10.1080/09687680802511774

Roach PCJ; Postis VLG; Deacon SE; Wright GSA; Ingram JC; Xia XB; McPherson MJ; Baldwin SA Large-scale preparation of bacterial cell membranes by tangential flow filtration MOL MEMBR BIOL 25 609-616, 2008
DOI:10.1080/09687680802530451

Kyle S; McPherson MJ; Ingham E; Aggeli A The self-assembling peptide, P as a scaffold for use in regenerative medicine European Cells and Materials 16 70-70, 2008

Knowles P; Kurtis C; Murray J; Saysell C; Tambyrajah W; Wilmot CM; McPherson MJ; Phillips SEV; Dooley DM; Brown D; Rogers M; Mure M Hydrazine and amphetamine binding to amine oxidases: old drugs with new prospects Journal of Neural Transmission (Budapest) 114 743-746, 2007
View abstract

Rogers MS; Tyler EM; Akyumani N; Kurtis CR; Spooner RK; Deacon SE; Tamber S; Firbank SJ; Mahmoud K; Knowles PF; Phillips SEV; McPherson MJ; Dooley DM The stacking tryptophan of galactose oxidase: A second-coordination sphere residue that has profound effects on tyrosyl radical behavior and enzyme catalysis BIOCHEMISTRY-US 46 4606-4618, 2007
DOI:10.1021/bi062139d

Rahman M; Ismat F; McPherson MJ; Baldwin SA Topology-informed strategies for the overexpression and purification of membrane proteins MOL MEMBR BIOL 24 407-U16, 2007
DOI:10.1080/09687860701243998

McPherson MJ; Møller SG PCR: The basics, 2006
View abstract

Mure M; Kurtis CR; Brown DE; Rogers MS; Tambyrajah WS; Saysell C; Wilmot CM; Phillips SEV; Knowles PF; Dooley DM; McPherson MJ Active site rearrangement of the 2-hydrazinopyridine adduct in Escherichia coli amine oxidase to an azo copper(II) chelate form: A key role for tyrosine 369 in controlling the mobility of the TPQ-2HP adduct BIOCHEMISTRY-US 44 1583-1594, 2005
DOI:10.1021/bi0479860

Bakhetia M; Charlton W; Atkinson HJ; McPherson MJ RNA interference of dual oxidase in the plant nematode Meloidogyne incognita MOL PLANT MICROBE IN 18 1099-1106, 2005

Bakhetia M; Charlton WL; Urwin PE; McPherson MJ; Atkinson HJ RNA interference and plant parasitic nematodes TRENDS PLANT SCI 10 362-367, 2005
DOI:10.1016/j.tplants.2005.06.007

Mure M; Brown DE; Saysell C; Rogers MS; Wilmot CM; Kurtis CR; McPherson MJ; Phillips SEV; Knowles PF; Dooley DM Role of the interactions between the active site base and the substrate Schiff base in amine oxidase catalysis. Evidence from structural and spectroscopic studies of the 2-hydrazinopyridine adduct of Escherichia coli amine oxidase BIOCHEMISTRY-US 44 1568-1582, 2005
DOI:10.1021/bi047988k

Mure M; Kurtis C; Brown D; Rogers M; Tambyrajah W; Saysell C; Phillips SEV; Knowles PF; Dooley DM; McPherson MJ Chelation of the 2-hydrazinopyridine Adduct I to Cu(II) (Adduct II) in Cu-containing amine oxidases. Direct evidence that Y369 controls the mobility of the TPQ and TPQ-derived reaction intermediates Biochemistry -, 2005

Yegutkin GG; Salminen T; Koskinen K; Kurtis C; McPherson MJ; Jalkanen S; Sallmi M A peptide inhibitor of vascular adhesion protein-1 (VAP-1) blocks leukocyte-endothelium interactions under shear stress EUR J IMMUNOL 34 2276-2285, 2004
DOI:10.1002/eji.200424932

Firbank S; Rogers M; Guerrero RH; Dooley DM; Halcrow MA; Phillips SE; Knowles PF; McPherson MJ Cofactor processing galactose oxidase FREE RADICALS: ENZYMOLOGY, SIGNALLING AND DISEASE 15-25, 2004

Wilmot CM; Saysell CG; Blessington A; Conn DA; Kurtis CR; McPherson MJ; Knowles PF; Phillips SEV Medical implications from the crystal structure of a copper-containing amine oxidase complexed with the antidepressant drug tranylcypromine FEBS LETT 576 301-305, 2004
DOI:10.1016/j.febslet.2004.09.031

Wilkinson D; Akumanyi N; Hurtado-Guerrero R; Dawkes H; Knowles PF; Phillips SEV; McPherson MJ Structural and kinetic studies of a series of mutants of galactose oxidase identified by directed evolution PROTEIN ENG DES SEL 17 141-148, 2004
DOI:10.1093/protein/gzh018

Deacon SE; Mahmoud K; Spooner RK; Firbank SJ; Knowles PF; Phillips SEV; McPherson MJ Enhanced fructose oxidase activity in a galactose oxidase variant CHEMBIOCHEM 5 972-979, 2004
DOI:10.1002/cbic.200300810

Firbank S; Rogers M; Guerrero RH; Dooley DM; Halcrow MA; Phillips SEV; Knowles PF; McPherson MJ Cofactor processing in galactose oxidase In Free Radicals: Enzymology, Signalling and Disease , 2004

Firbank SJ; Rogers M; Hurtado-Guerrero R; Dooley DM; Halcrow MA; Phillips SE; Knowles PF; McPherson MJ Cofactor processing in galactose oxidase. Biochem Soc Trans 31 506-509, 2003
View abstract

Atkinson HJ; Urwin PE; McPherson MJ Engineering plants for nematode resistance. Annu Rev Phytopathol 41 615-639, 2003
DOI:10.1146/annurev.phyto.41.052002.095737
View abstract

Firbank SJ; Rogers M; Hurtado-Guerrero R; Dooley DM; Halcrow MA; Phillips SEV; Knowles PF; McPherson MJ Cofactor processing in galactose oxidase BIOCHEM SOC T 31 506-509, 2003

Winter MD; McPherson MJ; Atkinson HJ Neuronal uptake of pesticides disrupts chemosensory cells of nematodes Parasitology 125 561-566, 2002

Saysell CG; Tambyrajah WS; Murray JM; Wilmot CM; Phillips SEV; McPherson MJ; Knowles PF Probing the catalytic mechanism of Escherichia coli amine oxidase using mutational variants and a reversible inhibitor as a substrate analogue BIOCHEM J 365 809-816, 2002
DOI:10.1042/BJ20011435

Winter MD; McPherson MJ; Atkinson HJ Neuronal uptake of pesticides disrupts chemosensory cells of nematodes PARASITOLOGY 125 561-565, 2002
DOI:10.1017/S0031182002002482

Rogers MS; Firbank SJ; Knowles PF; McPherson MJ; Phillips SEV; Dooley DM Biogenesis of the T Cys cofactor and the role of the pro-sequence in the copper-containing tyrosyl radical enzyme galactose oxidase Journal of Inorganic Biochemistry 86 pp.404-, 2001

Kurtis C; Mure M; Brown DA; Rogers MS; Dooley DM; Parsons MR; McPherson MJ Structure of the 2-hydrazinopyridine adduct II of E. coli amine oxidase Journal of Inorganic Biochemistry 86 pp.303-, 2001

Dooley DM; Firbank SJ; Rogers MS; Halcrow MA; McPherson MJ; Knowles PF; Phillips SEV Cofactor formation and pro-sequence cleavage in the copper containing tyrosyl radical enzyme galactose oxidase Journal of Inorganic Biochemistry 86 42-42, 2001

Kurtis C; Mure M; Brown DA; Rogers MS; Dooley DM; Parsons MR; McPherson MJ Structure of the 2-hydrazinopyridine adduct of II of E. coli amine oxidase Journal of Inorganic Biochemistry 86 pp.303-, 2001

McPherson MJ; Parsons MR; Wilmot CM Prokaryotic copper amine oxidases In Handbook of Metalloproteins , 2001

McPherson MJ; Parsons MR; Spooner RK; Wilmot CM Galactose oxidase In Handbook of Metalloproteins , 2001

McPherson MJ; Harrison DJ Protease inhibitors and directed evolution: enhancing plant resistance to nematodes BIOCHEM SOC SYMP 125-142, 2001
View abstract

Firbank SJ; Rogers MS; Dooley DM; Halcrow MA; Knowles PF; McPherson MJ; Phillips SEV Crystal structures of processing intermediates of the self-processing free-radical enzyme galactose oxidase J INORG BIOCHEM 86 220-220, 2001

Firbank SJ; Rogers MS; Wilmot CM; Dooley DM; Halcrow MA; Knowles PF; McPherson MJ; Phillips SEV Crystal structure of the precursor of galactose oxidase: An unusual self-processing enzyme P NATL ACAD SCI USA 98 12932-12937, 2001

Murray JM; Kurtis CR; Tambyrajah W; Saysell CG; Wilmot CM; Parsons MR; Phillips SEV; Knowles PF; McPherson MJ Conserved tyrosine-369 in the active site of Escherichia coli copper amine oxidase is not essential BIOCHEMISTRY-US 40 12808-12818, 2001
DOI:10.1021/bi011187p

Laurenzi M; Tipping AJ; Marcus SE; Knox JP; Federico R; Angelini R; McPherson MJ Analysis of the distribution of copper amine oxidase in cell walls of legume seedlings PLANTA 214 37-45, 2001

McPherson MJ; Harrison DJ Protease inhibitors and directed evolution: enhancing plant resistance to nematodes In From Proteins to New Enzymes , 2001

Urwin PE; Levesley A; McPherson MJ; Atkinson HJ Transgenic resistance to the nematode Rotylenchulus reniformis conferred by Arabidopsis thaliana plants expressing proteinase inhibitors MOL BREEDING 6 257-264, 2000

Rogers MS; Baron AJ; McPherson MJ; Knowles PF; Dooley DM Galactose oxidase pro-sequence cleavage and cofactor assembly are self-processing reactions J AM CHEM SOC 122 990-991, 2000

Saysell CG; Murray JM; Wilmot CM; Brown DE; Dooley DM; Phillips SEV; McPherson MJ; Knowles PF Investigation into the mechanism of lambda(max) shifts and their dependence on pH for the 2-hydrazinopyridine derivatives of two copper amine oxidases J MOL CATAL B-ENZYM 8 17-25, 2000

McPherson MJ; Møller SG PCR, 2000
View abstract

Murray JM; Saysell CG; Wilmot CM; Tambyrajah WS; Jaeger J; Knowles PF; Phillips SEV; McPherson MJ The active site base controls cofactor reactivity in Escherichia coli amine oxidase: X-ray crystallographic studies with mutational variants BIOCHEMISTRY-US 38 8217-8227, 1999

Wilmot CM; Hajdu J; McPherson MJ; Knowles PF; Phillips SEV Visualization of dioxygen bound to copper during enzyme catalysis SCIENCE 286 1724-1728, 1999

Wilmot CM; Hajdu J; Saysell CG; McPherson MJ; Knowles PF; Phillips SEV A movie of catalysis in the copper-containing quinoenzyme amine oxidase J INORG BIOCHEM 74 339-339, 1999

Moller SG; Urwin PE; Atkinson HJ; McPherson MJ Nematode-induced expression of atao1, a gene encoding an extracellular diamine oxidase associated with developing vascular tissue PHYSIOL MOL PLANT P 53 73-79, 1998

Moller SG; McPherson MJ Developmental expression and biochemical analysis of the Arabidopsis atao1 gene encoding an H2O2-generating diamine oxidase PLANT J 13 781-791, 1998

Urwin PE; McPherson MJ; Atkinson HJ Enhanced transgenic plant resistance to nematodes by dual proteinase inhibitor constructs PLANTA 204 472-479, 1998

Atkinson HJ; Lilley CJ; Urwin PE; McPherson MJ Engineering resistance to plant nematodes In The Physiology and Biochemistry of Free-living and Plant-parasitic Nematodes , 1998

Atkinson HJ; Lilley CJ; Urwin PE; McPherson MJ Transgenic crops for protection from nematodes In Pests and Diseases , 1998

Saysell CG; Barna T; Boreman CD; Baron AJ; McPherson MJ; Sykes AG Properties of the Trp290His variant of Fusarium NRRL 2903 galactose oxidase: interactions of the Goase(semi) state with different buffers, its redox activity and ability to bind azide. Journal of Biological Inorganic Chemistry 2 702-709, 1997

Appleby JM; Nelson G; McPherson MJ; Hamlyn PF Polymerase chain reaction amplification of the RuBisCo small subunit genes and their novel application to plant tissue identification HEREDITY 79 557-563, 1997

Saysell CG; Borman CD; Baron AJ; McPherson MJ; Sykes AG Kinetic Studies on the Redox Interconversion of GOase(semi) and GOase(ox) Forms of Galactose Oxidase with Inorganic Complexes as Redox Partners. Inorg Chem 36 4520-4525, 1997
View abstract

Urwin PE; Lilley CJ; McPherson MJ; Atkinson HJ Characterisation of two cDNAs encoding cysteine proteinases from the soybean cyst nematode Heterodera glycines Parasitology 114 605-613, 1997
View abstract

Saysell CG; Barna T; Borman CD; Baron AJ; McPherson MJ; Sykes AG Properties of the Trp290His variant of Fusarium NRRL 2903 galactose oxidase: interactions of the GOase(semi) state with different buffers, its redox activity and ability to bind azide J BIOL INORG CHEM 2 702-709, 1997

Urwin PE; Lilley CJ; McPherson MJ; Atkinson HJ Resistance to both cyst and root-knot nematodes conferred by transgenic Arabidopsis expressing a modified plant cystatin PLANT J 12 455-461, 1997

Lilley CJ; Urwin PE; Atkinson HJ; McPherson MJ Characterisation of cDNAs encoding serine proteinases from the soybean cyst nematode Molecular and Biochemical Parasitology 89 195-207, 1997
View abstract

Reynolds MP; Baron AJ; Wilmot CM; Vinecombe E; Stevens C; Phillips SEV; Knowles PF; McPherson MJ Structure and mechanism of galactose oxidase: Catalytic role of tyrosine 495 J BIOL INORG CHEM 2 327-335, 1997

Urwin PE; Moller SG; Lilley CJ; Atkinson HJ; McPherson MJ Continual GFP monitoring of CaMV35S promoter activity in nematode induced feeding cells in Arabidopsis thaliana Molecular Plant-Microbe Interactions 10 394-400, 1997

Wilmot CM; Murray JM; Alton G; Parsons MR; Convery MA; Blakeley V; Corner AS; Palcic MM; Knowles PF; McPherson MJ; Phillips SEV Catalytic mechanism of the quinoenzyme amine oxidase from Escherichia coli: Exploring the reductive half-reaction BIOCHEMISTRY-US 36 1608-1620, 1997

Atkinson HJ; Urwin PE; Lilley CJ; McPherson MJ Engineering transgenic resistance to potato cyst nematodes In Potato Cyst Nematodes , 1997

Urwin PE; Møller SG; Lilley CJ; McPherson MJ; Atkinson HJ Continual green-fluorescent protein monitoring of cauliflower mosaic virus 35S promoter activity in nematode-induced feeding cells in Arabidopsis thaliana. Mol Plant Microbe Interact 10 394-400, 1997
DOI:10.1094/MPMI.1997.10.3.394
View abstract

Lilley CJ; Urwin PE; Atkinson HJ; McPherson MJ Characterization of cDNAs encoding serine proteinases from the soybean cyst nematode Heterodera glycines. Mol Biochem Parasitol 89 195-207, 1997
View abstract

McPherson MJ; Urwin PE; Lilley CJ; Atkinson HJ Engineering plant nematode resistance by an anti-feedant approach In Cellular and Molecular Basis for Plant-Nematode Interactions , 1997

Atkinson HJ Image analysis of the growth of Globodera pallida and Meloidogyne incognita on transgenic tomato roots expressing cystatins Journal of Nematology 28 209-215, 1996

Lilley CJ; Urwin PE; McPherson MJ; Atkinson HJ Characterization of intestinally active proteinases of cyst-nematodes PARASITOLOGY 113 415-424, 1996

Atkinson HJ; Urwin PE; Clarke MC; McPherson MJ Image analysis of the growth of Globodera pallida and Meloidogyne incognita on transgenic tomato roots expressing cystatins J NEMATOL 28 209-215, 1996

Hansen E; Harper G; McPherson MJ; Atkinson HJ Differential expression patterns of the wound-inducible transgene wun1-uidA in potato roots following infection with either cyst or root knot nematodes PHYSIOL MOL PLANT P 48 161-170, 1996

Atkinson HJ Engineered Oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. The Plant Journal 8 121-131., 1995

Tipping AJ; McPherson MJ Cloning and molecular analysis of the pea seedling copper amine oxidase. J Biol Chem 270 16939-16946, 1995
View abstract

Urwin PE; Atkinson HJ; Waller DA; McPherson MJ Engineered oryzacystatin-I expressed in transgenic hairy roots confers resistance to Globodera pallida. Plant J 8 121-131, 1995
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Parsons MR; Convery MA; Wilmot CM; Yadav KD; Blakeley V; Corner AS; Phillips SE; McPherson MJ; Knowles PF Crystal structure of a quinoenzyme: copper amine oxidase of Escherichia coli at 2 A resolution. Structure 3 1171-1184, 1995
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Reynolds MP; Baron AJ; Wilmot CM; Phillips SE; Knowles PF; McPherson MJ Tyrosine 495 is a key residue in the active site of galactose oxidase. Biochem Soc Trans 23 510S-, 1995

Møller SG; McPherson MJ Molecular and functional studies of copper amine oxidase from Arabidopsis thaliana. Biochem Soc Trans 23 630S-, 1995

Urwin PE; Atkinson HJ; McPherson MJ Involvement of the NH2-terminal region of oryzacystatin-I in cysteine proteinase inhibition. Protein Eng 8 1303-1307, 1995
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ATKINSON HJ; URWIN PE; HANSEN E; MCPHERSON MJ DESIGNS FOR ENGINEERED RESISTANCE TO ROOT-PARASITIC NEMATODES TRENDS BIOTECHNOL 13 369-374, 1995

McPherson MJ; Taylor GR; Hames BD PCR 2, 1995
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Baron AJ; Stevens C; Wilmot C; Seneviratne KD; Blakeley V; Dooley DM; Phillips SE; Knowles PF; McPherson MJ Structure and mechanism of galactose oxidase. The free radical site. J Biol Chem 269 25095-25105, 1994
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Baron AJ; Wong TY; Hicks SJ; Gacesa P; Willcock D; McPherson MJ Alginate lyase from Klebsiella pneumoniae, subsp. aerogenes: gene cloning, sequence analysis and high-level production in Escherichia coli. Gene 143 61-66, 1994
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OGEL ZB; BRAYFORD D; MCPHERSON MJ CELLULOSE-TRIGGERED SPORULATION IN THE GALACTOSE OXIDASE-PRODUCING FUNGUS CLADOBOTRYUM (DACTYLIUM) DENDROIDES NRRL-2903 AND ITS REIDENTIFICATION AS A SPECIES OF FUSARIUM MYCOL RES 98 474-480, 1994

Atkinson HJ; Blundy KS; Clarke MC; Hansen E; Harper G; Koristas V; McPherson MJ; O'Reilly D; Scollan C; Turnbull SR; Urwin PE Novel plant defences against nematodes In Advances in Molecular Plant Nematology , 1994

Baron AJ; Wong TY; Hicks SJ; Gacesa P; Willcock D; McPherson MJ Algillate lyase from Klebsiella, pneumoniae, subsp. Aerogenes: Gene cloning, sequence analysis and high-level production in Escherichia coli Gene 143 61-66, 1994
DOI:10.1016/0378-1119(94)90605-X
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NOVITSKI CE; BROWN S; CHEN R; CORNER AS; ATKINSON HJ; MCPHERSON MJ MAJOR SPERM PROTEIN GENES FROM GLOBODERA-ROSTOCHIENSIS J NEMATOL 25 548-554, 1993

McPherson MJ; Parish JH Chemical and biochemical manipulations of nucleic acids. Nat Prod Rep 10 175-197, 1993

Tipping AJ; McPherson MJ Cloning and sequence analysis of a pea seedling amine oxidase gene. Biochem Soc Trans 21 ( Pt 3) 317S-, 1993

Baron AJ; Stevens C; Wilmot CM; Knowles PF; Phillips SE; McPherson MJ Preliminary studies of two active site mutants of galactose oxidase. Biochem Soc Trans 21 ( Pt 3) 319S-, 1993

McPherson MJ; Stevens C; Baron AJ; Ogel ZB; Seneviratne K; Wilmot C; Ito N; Brocklebank I; Phillips SE; Knowles PF Galactose oxidase: molecular analysis and mutagenesis studies. Biochem Soc Trans 21 ( Pt 3) 752-756, 1993

Korber FC; Rizkallah PJ; Attwood TK; Wootton JC; McPherson MJ; North AC; Geddes AJ; Abeysinghe IS; Baker PJ; Dean JL Crystallization of the NADP(+)-dependent glutamate dehydrogenase from Escherichia coli. J Mol Biol 234 1270-1273, 1993
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Jones KM; McPherson MJ; Baron AJ; Mattaj IW; Riordan CL; Wootton JC The gdhA1 point mutation in Escherichia coli K12 CLR207 alters a key lysine residue of glutamate dehydrogenase. Mol Gen Genet 240 286-289, 1993
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Baron AJ; Stevens C; Wilmot CM; Knowles PF; Phillips SEV; McPherson MJ Preliminary studies of two active site mutants of galactose oxidase Biochemical Society Transactions 21 -, 1993

Tipping AJ; McPherson MJ Cloning and sequence analysis of a pea seedling amine oxidase gene Biochemical Society Transactions 21 -, 1993

Ito N; Phillips SE; Stevens C; Ogel ZB; McPherson MJ; Keen JN; Yadav KD; Knowles PF Three-dimensional structure of galactose oxidase: an enzyme with a built-in secondary cofactor. Faraday Discuss 75-84, 1992
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Wardhan H; McPherson MJ; Harris CA; Sharma E; Sastry GR Molecular analysis of the recA gene of Agrobacterium tumefaciens C58. Gene 121 133-136, 1992
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Ogel ZB; McPherson MJ Efficient deletion mutagenesis by PCR. Protein Eng 5 467-468, 1992

McPherson MJ; Ogel ZB; Stevens C; Yadav KD; Keen JN; Knowles PF Galactose oxidase of Dactylium dendroides. Gene cloning and sequence analysis. J Biol Chem 267 8146-8152, 1992
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Smallwood MF; Gurr SJ; McPherson MJ; Roberts K; Bowles DJ The pattern of plant annexin gene expression. Biochem J 281 ( Pt 2) 501-505, 1992
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TELLER JK; SMITH RJ; MCPHERSON MJ; ENGEL PC; GUEST JR THE GLUTAMATE-DEHYDROGENASE GENE OF CLOSTRIDIUM-SYMBIOSUM - CLONING BY POLYMERASE CHAIN-REACTION, SEQUENCE-ANALYSIS AND OVER-EXPRESSION IN ESCHERICHIA-COLI EUR J BIOCHEM 206 151-159, 1992

Gurr SJ; McPherson MJ Nucleic acid preparation and hybridisation In Molecular Plant Pathology : A Practical Approac , 1992

McPherson MJ; Oliver RP; Gurr SJ The polymerase chain reaction In Molecular Plant Pathology : A Practical Approach , 1992

Gurr SJ; McPherson MJ; Bowles DJ Molecular plant pathology, 1992
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Gurr SJ; McPherson MJ; Bowles DJ Molecular plant pathology, 1992
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Ogel ZB; McPherson MJ Protocol. Efficient deletion mutagenesis by PCR Protein Engineering 5 467-468, 1992

Ito N; Phillips SE; Stevens C; Ogel ZB; McPherson MJ; Keen JN; Yadav KD; Knowles PF Novel thioether bond revealed by a 1.7 A crystal structure of galactose oxidase. Nature 350 87-90, 1991
DOI:10.1038/350087a0
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Gurr SJ; McPherson MJ; Scollan C; Atkinson HJ; Bowles DJ Gene expression in nematode-infected plant roots. Mol Gen Genet 226 361-366, 1991
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McPherson MJ Construction and screening of PCR-based cDNA libraries In PCR : A Practical Approach , 1991

McPherson MJ; Jones KM; Gurr SJ PCR with highly degenerate primers In PCR : A Practical Approach , 1991

McPherson MJ Directed mutagenesis, 1991

McPherson MJ; Quirke P PCR, 1991
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Ito N; Keen JN; Knowles PF; McPherson MJ; Phillips SE; Stevens C; Yadav KD Structural analysis of galactose oxidase. Biochem Soc Trans 18 931-932, 1990

Lightfoot DA; McPherson MJ; Wootton JC Detection of a homologue to an E. coli glutamate synthase gene in a cyanobacterium. FEMS Microbiol Lett 48 129-132, 1989
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Wardhan H; McPherson MJ; Sastry GR Identification, cloning, and sequence analysis of the nitrogen regulation gene ntrC of Agrobacterium tumefaciens C58. Mol Plant Microbe Interact 2 241-248, 1989
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KOHLUAR SI; PARISH JH; BLEASBY AJ; PAPPIN DJC; AINLEY K; JOHANSEN FE; MCPHERSON MJ; RADFORD A EXPORTED PROTEINS OF NEUROSPORA-CRASSA - 1-GLUCOAMYLASE ENZYME MICROB TECH 11 692-695, 1989

LIGHTFOOT DA; MCPHERSON MJ; WOOTTON JC DETECTION OF A HOMOLOG TO AN ESCHERICHIA-COLI GLUTAMATE SYNTHASE GENE IN A CYANOBACTERIUM FEMS MICROBIOL LETT 57 129-132, 1989

Charalambous BM; Keen JN; McPherson MJ Collagen-like sequences stabilize homotrimers of a bacterial hydrolase. EMBO J 7 2903-2909, 1988
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McPherson MJ; Baron AJ; Jones KM; Price GJ; Wootton JC Multiple interactions of lysine-128 of Escherichia coli glutamate dehydrogenase revealed by site-directed mutagenesis studies. Protein Eng 2 147-152, 1988
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CHARALAMBOUS BM; MCPHERSON MJ MULTIMOLECULAR ORGANIZATION OF THE BACTERIAL ENZYME PULLULANASE BIOCHEM SOC T 16 722-723, 1988

MCPHERSON MJ; CHARALAMBOUS BM FUNCTIONAL-ANALYSIS OF THE STARCH DEBRANCHING ENZYME PULLULANASE BIOCHEM SOC T 16 723-724, 1988

JONES KM; BARON AJ; WOOTTON JC; MCPHERSON MJ SITE-DIRECTED MUTAGENESIS STUDIES OF ESCHERICHIA-COLI GLUTAMATE-DEHYDROGENASE BIOCHEM SOC T 16 874-875, 1988

McPherson MJ; Parish JH Applications of recombinant DNA in biotechnology. Nat Prod Rep 4 205-224, 1987

JONES KM; MCPHERSON MJ; BARON AJ; WOOTTON JC INVESTIGATION OF THE CATALYTIC MECHANISM OF GLUTAMATE-DEHYDROGENASE WITH SITE-DIRECTED MUTANTS PROTEIN ENG 1 259-260, 1987

PARISH JH; MCPHERSON MJ CHEMICAL AND BIOCHEMICAL MANIPULATION OF DNA AND THE EXPRESSION OF FOREIGN GENES IN MICROORGANISMS NAT PROD REP 4 139-156, 1987

MOUNTAIN A; MCPHERSON MJ; BARON AJ; WOOTTON JC THE KLEBSIELLA AEROGENES GLUTAMATE-DEHYDROGENASE (GDHA) GENE - CLONING, HIGH-LEVEL EXPRESSION AND HYBRID ENZYME FORMATION IN ESCHERICHIA-COLI MOL GEN GENET 199 141-145, 1985

MCPHERSON MJ; BARON AJ; PAPPIN DJC; WOOTTON JC RESPIRATORY NITRATE REDUCTASE OF ESCHERICHIA-COLI - SEQUENCE IDENTIFICATION OF THE LARGE SUBUNIT GENE FEBS LETT 177 260-264, 1984

Wootton JC; McPherson MJ The Genetic Manipulation of Plants and its' Application to Agriculture In Genes of Nitrogen Assimilation , 1984

MCPHERSON MJ; WOOTTON JC COMPLETE NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI GDHA GENE NUCLEIC ACIDS RES 11 5257-5266, 1983

MATTAJ IW; MCPHERSON MJ; WOOTTON JC LOCALIZATION OF A STRONGLY CONSERVED SECTION OF CODING SEQUENCE IN GLUTAMATE-DEHYDROGENASE GENES FEBS LETT 147 21-25, 1982

Yuzugullu Y; Trinh CH; Smith MA; Pearson AR; Sutay Kocabas D; Phillips SEV; Bakir U; Ogel ZB; McPherson MJ Crystal structure, recombinant expression and mutagenesis studies of the bifunctional catalase-phenol oxidase from Scytalidium thermophilum Acta Crystallographica Section D: Biological Crystallography 69 398-408,