Faculty of Biological Sciences

Dr Andrew Tuplin

BSc, PhD 2004, Edinburgh
Lecturer in Molecular and Cellular Biology
School of Molecular and Cellular Biology

Contact:  Garstang 8.58, +44(0) 113 34 35582, email address for  

You can read more about Dr Tuplin's interests here:
http://www.astbury.leeds.ac.uk/people/staff/staffpage.php?StaffID=AKT

Research Interests

Control of replication and translation in positive-strand RNA viruses.

Research in my group is focused on investigating how RNA viruses (particularly arboviruses) control  replication and translation of their genomes. Specifically, interactions between viral RNA structures, host cell proteins and non-coding RNA, and the potential of such interactions as novel therapeutic targets.

We predominantly work on flaviviruses (Dengue and Zika virus) and Chikungunya virus. 

  • Dengue, Zika and chikungunya viruses are mosquito transmited arboviruses and significant human pathogens.
  • Their genomes encode  RNA structures essential for replication in vertebrate and invertebrate host cells.
  • No vaccine or specific antiviral therapy is available for  dengue, zika or chikungunya virus.
  • The geographic range off all three viruses is increasing.

 

Our research is funded by the MRC, BBSRC and The Royal Society.

 

Current Projects

   

1:  Interactions and functional mechanisms of arbovirus RNA genome structures:

Comparing...   

  • Vertebrate and invertebrate hosts.
  • Alternative cellular compartments.
  • Different points of the virus life cycle.

In order to...

  • Dissect the structure, function and mechanisms by which dynamic RNA structures within viral genomes interact to control essential aspects of the virus life cycle. 
  • Investigate the potential of viral RNA structures or their interacting partners as novel antiviral therapeutic targets.

 

2:  The role of host ion channels during chikungunya virus genome replication:

 

3:  Recognition and targeting of specific viral RNA structures within the intracellular environment:

 

PhD opportunities: 

Funded PhD's - deadline Janurary 5th 2018

The role of chikungunya virus nsP1 protein in virus replication:

This is a fully funded PhD project, advertised as part of the BBSRC White Rose Mechanistic Biology DTP 4 year studentship: The deadline for applications is January 5th 2018.

For details of the project, funding and how to apply please follow the link below to the advert on findaPhD.com.

https://www.findaphd.com/search/ProjectDetails.aspx?PJID=49356

 

Self-funded or scholarship studentsAPPLICATIONS ACCEPTED YEAR ROUND.

I actively encourage and support applications from International self-funded or scholarship/fellowship PhD or MSc students. International students must have a good command of both written and spoken English. Applications can be made throughout the year.

For further information, including international studentship fees and bench costs see the following links:

 

https://www.findaphd.com/search/ProjectDetails.aspx?PJID=49355

http://www.fbs.leeds.ac.uk/gradschool/research/index.php?tab=1

 

 

Faculty Research and Innovation



Studentship information

Undergraduate project topics:

  • Elucidate the structure and interactions of dynamic RNA structural elements within virus genomes.
  • Dissect the function and mechanisms by which RNA/protein interactions control essential aspects of the virus life cycle.
  • Investigate the potential of RNA elements as novel antiviral therapeutic targets using structure led inhibitor design.

Postgraduate studentship areas:

  • Elucidate the structure and interactions of dynamic RNA structural elements within virus genomes.
  • Dissect the function and mechanisms by which RNA/protein interactions control essential aspects of the virus life cycle.
  • Investigate the potential of RNA elements as novel antiviral therapeutic targets using structure led inhibitor design.
  • The role of host ion channels during chikungunya virus genome replication.

See also:

Modules managed

BIOL1305 - Integrated Skills in Biosciences 1

Modules taught

BIOC3160 - Laboratory/Literature/Computing Research Project
BIOC3900 - Cancer Biology
BIOL1302/1303/MICR1320 - Intro skills - Biological Science and Microbiology
BIOL1305 - Integrated Skills in Biosciences 1
BIOL3306 - Biological Sciences Research Project
BIOL5294M - MSc Bioscience Research Project Proposal
BIOL5392M - Bioscience MSc Research Project
BLGY1124/1128 - The Diversity of Life/Living Planet
BLGY1128 - Living Planet
MICR2121 - Molecular Virology
MICR2320/MICR3343 - Skills for Microbiology i
MICR3211/BIOL3210 A - ATU - emerging infections

Centre membership: The Astbury Centre for Structural Molecular Biology

Group Leader Dr Andrew Tuplin  (Lecturer in Molecular and Cellular Biology)

Control of replication and translation in positive-strand RNA viruses. 

Miss Henna Khalid  (Research Technician )

Dr Marietta Müller  (Research Fellow)


Postgraduates

Lauren Branfield (Primary supervisor) 34% FTE
Catherine Kendall (Primary supervisor) 65% FTE
Emily Levitt (Primary supervisor) 50% FTE
Oliver Prosser (Primary supervisor) 50% FTE
Ghada Shawli (Primary supervisor) 50% FTE
Yanni Gao (Co-supervisor) 50% FTE
Raymond Li (Co-supervisor) 50% FTE

Khasnatinov MA, Tuplin A, Gritsun DJ, Slovak M, Kazimirova M, Lickova M, Havlikova S, Klempa B, Labuda M, Gould EA, Gritsun TS Tick-borne encephalitis virus structural proteins are the primary viral determinants of non-viraemic transmission between ticks whereas non-structural proteins affect cytotoxicity PLoS ONE 11 -, 2016
DOI:10.1371/journal.pone.0158105
View abstract

Stewart H, Bingham RJ, White SJ, Dykeman EC, Zothner C, Tuplin AK, Stockley PG, Twarock R, Harris M Identification of novel RNA secondary structures within the hepatitis C virus genome reveals a cooperative involvement in genome packaging Scientific Reports 6 -, 2016
DOI:10.1038/srep22952
View abstract

Tuplin AK Diverse roles and interactions of RNA structures during the replication of positive-stranded RNA viruses of humans and animals Journal of General Virology 96 1497-1503, 2015
DOI:10.1099/vir.0.000066
View abstract

Tuplin A, Struthers M, Cook J, Bentley K, Evans DJ Inhibition of HCV translation by disrupting the structure and interactions of the viral CRE and 3' X-tail Nucleic Acids Research 43 2914-2926, 2015
DOI:10.1093/nar/gkv142
View abstract

Tuplin A, Evans DJ Compounds that target structures within the HCV genome of multiple viral genotypes and modulate HCV translation and replication: International patent application. PCT/GB/2014/050943., 2014
View abstract

Tuplin A, Struthers M, Simmonds P, Evans DJ A twist in the tail: SHAPE mapping of long-range interactions and structural rearrangements of RNA elements involved in HCV replication. Nucleic Acids Research 40 6908-6921, 2012
DOI:10.1093/nar/gks370
View abstract

Tuplin A, Evans DJ, Buckley A, Jones IM, Gould EA, Gritsun TS Replication enhancer elements within the open reading frame of tick-borne encephalitis virus and their evolution within the Flavivirus genus. Nucleic Acids Res 39 7034-7048, 2011
DOI:10.1093/nar/gkr237
View abstract

Diviney S, Tuplin A, Struthers M, Armstrong V, Elliott RM, Simmonds P, Evans DJ A hepatitis C virus cis-acting replication element forms a long-range RNA-RNA interaction with upstream RNA sequences in NS5B. J Virol 82 9008-9022, 2008
DOI:10.1128/JVI.02326-07
View abstract

Gritsun TS, Tuplin A, Gould EA Origin, Evolution and Function of Flavivirus RNA in Untranslated and Coding Regions: Implications for Virus Transmission. In: Molecular Biology of the Flavivirus. Editors: Kalitzky M and Borowski P. Norwich, UK: Horizon Scientific Press; pp. 47–99., 2006

Tuplin A, Evans DJ, Simmonds P Detailed mapping of RNA secondary structures in core and NS5B-encoding region sequences of hepatitis C virus by RNase cleavage and novel bioinformatic prediction methods. J Gen Virol 85 3037-3047, 2004
DOI:10.1099/vir.0.80141-0
View abstract

Simmonds P, Tuplin A, Evans DJ Detection of genome-scale ordered RNA structure (GORS) in genomes of positive-stranded RNA viruses: Implications for virus evolution and host persistence. RNA 10 1337-1351, 2004
DOI:10.1261/rna.7640104
View abstract

Tuplin A, Wood J, Evans DJ, Patel AH, Simmonds P Thermodynamic and phylogenetic prediction of RNA secondary structures in the coding region of hepatitis C virus. RNA 8 824-841, 2002
View abstract

Cuceanu NM, Tuplin A, Simmonds P Evolutionarily conserved RNA secondary structures in coding and non-coding sequences at the 3' end of the hepatitis G virus/GB-virus C genome. J Gen Virol 82 713-722, 2001
DOI:10.1099/0022-1317-82-4-713
View abstract