Dr Christian Tiede

Research Fellow
School of Molecular and Cellular Biology

Contact: Astbury 7.111, +44(0) 113 34 31402, email address for  

Lopata A, Hughes R, Tiede C, Heissler SM, Sellers JR, Knight PJ, Tomlinson D, Peckham M Affimer proteins for F-actin: Novel affinity reagents that label F-actin in live and fixed cells Scientific Reports 8, 2018
DOI:10.1038/s41598-018-24953-4
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Schlichthaerle T, Eklund A, Schueder F, Strauss M, Tiede C, Curd A, Ries J, Peckham M, Tomlinson D, Jungmann R Site-specific labeling of Affimers for DNA-PAINT microscopy. Angewandte Chemie (International ed. in English), 2018
DOI:10.1002/anie.201804020
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Hughes DJ, Tiede C, Penswick N, Tang AAS, Trinh CH, Mandal U, Zajac KZ, Gaule T, Howell G, Edwards TA, Duan J, Feyfant E, McPherson MJ, Tomlinson DC, Whitehouse A Generation of specific inhibitors of SUMO-1- and SUMO-2/3-mediated protein-protein interactions using Affimer (Adhiron) technology Science Signaling 10, 2017
DOI:10.1126/scisignal.aaj2005
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Tiede C, Bedford R, Heseltine SJ, Smith G, Wijetunga I, Ross R, Alqallaf D, Roberts APE, Balls A, Curd A, Hughes RE, Martin H, Needham SR, Zanetti-Domingues LC, Sadigh Y, Peacock TP, Tang AA, Gibson N, Kyle H, Platt GW, Ingram N, Taylor T, Coletta LP, Manfield I, Knowles M, Bell S, Esteves F, Maqbool A, Prasad RK, Drinkhill M, Bon RS, Patel V, Goodchild SA, Martin-Fernandez M, Owens RJ, Nettleship JE, Webb ME, Harrison M, Lippiat JD, Ponnambalam S, Peckham M, Smith A, Ferrigno PK, Johnson M, McPherson MJ, Tomlinson DC Affimer proteins are versatile and renewable affinity reagents eLife 6, 2017
DOI:10.7554/eLife.24903
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Tiede C, Tang AAS, Deacon SE, Mandal U, Nettleship JE, Owen RL, George SE, Harrison DJ, Owens RJ, Tomlinson DC, McPherson MJ Adhiron: a stable and versatile peptide display scaffold for molecular recognition applications. Protein Eng Des Sel 27 145-155, 2014
DOI:10.1093/protein/gzu007
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Zhurauski P, Arya SK, Jolly P, Tiede C, Tomlinson DC, Ko Ferrigno P, Estrela P Sensitive and selective Affimer-functionalised interdigitated electrode-based capacitive biosensor for Her4 protein tumour biomarker detection Biosensors and Bioelectronics 108 1-8, 2018
DOI:10.1016/j.bios.2018.02.041
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Robinson JI, Baxter EW, Owen RL, Thomsen M, Tomlinson DC, Waterhouse MP, Win SJ, Nettleship JE, Tiede C, Foster RJ, Owens RJ, Fishwick CWG, Harris SA, Goldman A, McPherson MJ, Morgan AW Affimer proteins inhibit immune complex binding to FcγRIIIa with high specificity through competitive and allosteric modes of action Proceedings of the National Academy of Sciences of the United States of America 115 E72-E81, 2018
DOI:10.1073/pnas.1707856115
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Xie C, Tiede C, Zhang X, Wang C, Li Z, Xu X, McPherson MJ, Tomlinson DC, Xu W Development of an Affimer-antibody combined immunological diagnosis kit for glypican-3 Scientific Reports 7, 2017
DOI:10.1038/s41598-017-10083-w
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Tang AAS, Tiede C, Hughes DJ, McPherson M, Tomlinson DC Isolation of isoform-specific binding proteins (Affimers) by phage display using negative selection Science Signaling 10, 2017
DOI:10.1126/scisignal.aan0868
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Bedford R, Tiede C, Hughes R, Curd A, McPherson MJ, Peckham M, Tomlinson DC Alternative reagents to antibodies in imaging applications, 2017
DOI:10.1007/s12551-017-0278-2
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Tiede C, Tang A, Taylor T, Tomlinson D, Mcpherson M Affimers are versatile and renewable molecular recognition reagents, 2017

Hughes R, Lopata A, Balls A, Tiede C, Tomlinson D, Curd A, Peckham M Using small non-antibody binding proteins to visualise the cytoskeleton in super-resolution microscopy, 2017

Wang W, Guo Y, Tiede C, Chen S, Kopytynski M, Kong Y, Kulak A, Tomlinson D, Chen R, McPherson M, Zhou D Ultraefficient Cap-Exchange Protocol To Compact Biofunctional Quantum Dots for Sensitive Ratiometric Biosensing and Cell Imaging ACS Applied Materials and Interfaces 9 15232-15244, 2017
DOI:10.1021/acsami.6b13807
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Koutsoumpeli E, Tiede C, Murray J, Tang A, Bon RS, Tomlinson DC, Johnson S Antibody Mimetics for the Detection of Small Organic Compounds Using a Quartz Crystal Microbalance Analytical Chemistry 89 3051-3058, 2017
DOI:10.1021/acs.analchem.6b04790
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King RJ, Schuett K, Tiede C, Jankowski V, John V, Trehan A, Simmons K, Ponnambalam S, Fishwick CWG, McPherson MJ, Tomlinson DC, Ajjan RA COMPLEMENT C3 IN DIABETES: HYPOFIBRINOLYSIS, GLYCATION AND INHIBITING INTERACTION WITH FIBRINOGEN TO REDUCE CARDIOVASCULAR RISK, 2016
DOI:10.1136/heartjnl-2016-310696.47

Robinson J, Baxter E, Tomlinson D, Foster R, Owen R, Win S, Nettleship J, Tiede C, Kankanala J, Owens R, Fishwick C, McPherson M, Morgan A Novel Agents for Blocking the Interaction of Immune Complexes with the Activatory Fc gamma RIIIa Receptor, 2016

King RJ, Schuett K, Tiede C, Jankowski V, John V, Simmons K, Ponnambalam S, Fishwick C, McPherson M, Tomlinson D, Ajjan R Complement C3 and hypofibrinolysis in diabetes: role of protein glycation and development of a new methodology to reduce thrombosis risk, 2016

Baxter EW, Robinson JI, Tomlinson DC, Foster RJ, Owen RL, Win SJ, Nettleship JE, Tiede C, Kankanala J, Owens RJ, Fishwick CWG, McPherson MJ, Morgan AW NOVEL AGENTS FOR BLOCKING THE INTERACTION OF IMMUNE COMPLEXES WITH THE ACTIVATORY FC gamma RIIIA RECEPTOR, 2016
DOI:10.1136/annrheumdis-2016-209124.139

Fisher MJ, Williamson DJ, Burslem GM, Plante JP, Manfield IW, Tiede C, Ault JR, Stockley PG, Plein S, Maqbool A, Tomlinson DC, Foster R, Warriner SL, Bon RS Trivalent Gd-DOTA reagents for modification of proteins RSC Advances 5 96194-96200, 2015
DOI:10.1039/c5ra20359g
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Puard V, Tan K-Y, Baldwin K, Carrol E, Patisson R, Beynon R, Tiede C, McPherson M, Tomlinson D, Ferrigno PK Affimers, new affinity reagents for life science research, 2015

Kyle HF, Wickson KF, Stott J, Burslem GM, Breeze AL, Tiede C, Tomlinson DC, Warriner SL, Nelson A, Wilson AJ, Edwards TA Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies Molecular BioSystems 11 2738-2749, 2015
DOI:10.1039/c5mb00284b
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King R, Tiede C, Simmons K, Fishwick C, Schuett K, Kearney K, Tomlinson D, Ajjan R Inhibition of complement C3 and fibrinogen interaction: a potential novel therapeutic target to reduce fibrin-related thrombosis risk, 2015

Raina M, Sharma R, Deacon SE, Tiede C, Tomlinson D, Davies AG, McPherson MJ, Wälti C Antibody mimetic receptor proteins for label-free biosensors Analyst 140 803-810, 2015
DOI:10.1039/c4an01418a
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King R, Tiede C, McPherson M, Manfield I, Fishwick C, Tomlinson D, Ajjan R A novel methodology for modulation of fibrin clot lysis: the role of fibrinogen-targeted artificial binding proteins, 2013

Stadler LKJ, Hoffmann T, Tomlinson DC, Song Q, Lee T, Busby M, Nyathi Y, Gendra E, Tiede C, Flanagan K, Cockell SJ, Wipat A, Harwood C, Wagner SD, Knowles MA, Davis JJ, Keegan N, Ferrigno PK Structure-function studies of an engineered scaffold protein derived from Stefin A. II: Development and applications of the SQT variant. Protein Eng Des Sel 24 751-763, 2011
DOI:10.1093/protein/gzr019
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Paschke M, Tiede C, Höhne W Erratum: Engineering a circularly permuted GFTP scaffold for peptide presentation (J. Mol. Recognit. (2007) 20, 5, (367-378)). Journal of Molecular Recognition 22, 2009
DOI:10.1002/jmr.867

Paschke M, Tiede C, Höhne W Engineering a circularly permuted GFP scaffold for peptide presentation Journal of Molecular Recognition 20 367-378, 2007
DOI:10.1002/jmr.844
View abstract