Faculty of Biological Sciences

Dr David Marples

BA, BM, BCh, MA, DPhil, Oxford. PhD 1990
Senior Lecturer in Physiology & Anatomy
School of Biomedical Sciences

Background: I trained as a medic at Oxford, and did a D.Phil. in physiology with Ann Taylor before finishing my medical training. After my house jobs, I got back to research as soon as possible... I spent another two years as a post-doc with Ann, studying the role of the cytoskeleton in the response to vasopressin. I then spent two years in Aarhus, Denmark, working with Soren Nielsen, mainly on the newly-discovered aquaporin-2 (AQP2) water channel. I am now continuing this work, often in collaboration with Soren, in Leeds.

Contact:  Garstang 5.55e | +44(0) 113 34 34245 | email address for  

Research Interests

Regulation of shuttling and expression of aquaporin 2 water channels

Aquaporin-2 water channels are shuttled from a store in vesicles to the apical plasma membrane in response to vasopressin. One half of our research is focussed on the cellular apparatus involved in this regulated exocytic process, for which cAMP, rather than calcium, is the trigger. The other major strand concerns the cellular signals which regulate AQP2 levels within the cells. This is important in the pathogenesis of a number of water balance disorders. A final interest is the organisation of microtubules within epithelial cells, and their role in regulated vesicular trafficking, including that of AQP2.

 

Current Projects

  • Cytoskeletal involvement in the trafficking of AQP2
  • Role of prostaglandins in the regulation of AQP2 expression
  • Localisation and function of TASK channels in the kidney (with Malcolm Hunter)
  • Localisation of Trp channels in small arteries (with David Beech)
  • Changes in sino-atrial node ion channels during ageing (with Mark Boyett)
 

Studentship information

Postgraduate studentship areas:

  • Role of aquaporins in the regulation of water balance.

See also:

Admin roles

External Examiner (taught course): Imperial College, London
FBS UGTSEC
UG Programme Leader: Medical Sciences

Modules managed

BMSC1110 - Foundations of Biomedical Sciences
BMSC2225 - Physiology of Absorption and Excretion
SPSC1107 - Functional Anatomy - Sport Sci

Modules taught

BMSC1103 - Basic Laboratory and Scientific Skills
BMSC1110 - Foundations of Biomedical Sciences
BMSC1110/SPSC1220 - Foundation modules
BMSC1213 - Basic Laboratory and Scientific Skills 2
BMSC1214 - Human Endocrinology
BMSC2225 - Physiology of Absorption and Excretion
BMSC2229 - Exp Skills: Medical Sciences
BMSC3143/44/45/46 - ATUs - BMS specific
BMSC3233/34/35/36/37 - ATUs - BMS specific
BMSC3301 - Research Proj: Biomed Sciences
MEDI1203 - Nutrition and Energy
SPSC1107 - Functional Anatomy - Sport Sci
SPSC3061 - Research Project in Sport and Exercise Science II

Committees

Member of Undergraduate School Taught Student Education Committee

Group Leader Dr David Marples  (Senior Lecturer in Physiology & Anatomy)

Regulation of shuttling and expression of aquaporin 2 water channels 


Mobasheri A; Kendall BH; Maxwell JEJ; Sawran AV; German AJ; Marples D; Luck MR; Royal MD Cellular localization of aquaporins along the secretory pathway of the lactating bovine mammary gland: An immunohistochemical study ACTA HISTOCHEM 113 137-149, 2011
DOI:10.1016/j.acthis.2009.09.005

Baggaley E; Nielsen S; Marples D Dehydration-induced increase in aquaporin-2 protein abundance is blocked by nonsteroidal anti-inflammatory drugs AM J PHYSIOL-RENAL 298 F1051-F1058, 2010
DOI:10.1152/ajprenal.90202.2008

Mobasheri A; Moskaluk CA; Marples D; Shakibaei M Expression of aquaporin 1 (AQP1) in human synovitis ANN ANAT 192 116-121, 2010
DOI:10.1016/j.aanat.2010.01.001

Mobasheri A; Cathro HP; German A; Marples D; Martín-vasallo P; Canessa CM Applications of Tissue Microarrays in Renal Physiology and Pathology, 2009
DOI:10.1002/9783527628612.ch8

Lutken SC; Kim SW; Jonassen T; Marples D; Knepper MA; Kwon TH; Frokiaer J; Nielsen S Changes of renal AQP2, ENaC, and NHE3 in experimentally induced heart failure: response to angiotensin II AT(1) receptor blockade AM J PHYSIOL-RENAL 297 F1678-F1688, 2009
DOI:10.1152/ajprenal.00010.2009

Richardson SM; Knowles R; Hoyland JA; Marples D; Mobasheri A Aquaporin expression in the human intervertebral disc Journal of Molecular Histology 39 303-309, 2008
DOI:10.1007/s10735-008-9166-1
View abstract

Floyd RV; Mason SL; Proudman CJ; German AJ; Marples D; Mobasheri A Expression and nephron segment-specific distribution of major renal aquaporins (AQP1-4) in Equus caballus, the domestic horse American Journal of Physiology - Regulatory Integrative and Comparative Physiology 293 -, 2007
DOI:10.1152/ajpregu.00689.2005
View abstract

Mobasheri A; Marples D; Young IS; Floyd RV; Moskaluk CA; Frigeri A Distribution of the AQP4 water channel in normal human tissues - Protein and tissue microarrays reveal expression in several new anatomical locations, including the prostate gland and seminal vesicles CHANNELS 1 -, 2007

Mobasheri A; Marples D; Frigeri A Distribution of the aquaporin water channels AQP1, AQP3, AQP4, and AQP9 in the human choroid plexus, 2005

Mobasheri A; Marples D; Young I; Moskaluk C; Frigeri A Tissue distribution of the AQP-4 water channel: A study using normal human Tissue MicroArrays, 2005

Mobasheri A; Airley R; Hewitt SM; Marples D Heterogeneous expression of the aquaporin 1 (AQP1) water channel in tumors of the prostate, breast, ovary, colon and lung: A study using high density multiple human tumor tissue microarrays INT J ONCOL 26 1149-1158, 2005

Shaw S; Marples D N-ethylmaleimide causes aquaporin-2 trafficking in the renal inner medullary collecting duct by direct activation of protein kinase A AM J PHYSIOL-RENAL 288 F832-F839, 2005
DOI:10.1152/ajprenal.00041.2004

Mobasheri A; Marples D; Young I; Moskaluk C; Frigeri A Distribution of the AQP-4 water channel in normal human tissue microarrays, 2005

Mobasheri A; Wray S; Marples D Distribution of AQP2 and AQP3 water channels in human tissue microarrays J MOL HISTOL 36 1-14, 2005
DOI:10.1007/s10735-004-2633-4

Shaw S; Marples DDR N-ethylmaleimide (NEM) causes an aquaporin-2 trafficking in the renal inner medullary collecting duct by direct activation of protein kinase A American Journal of Physiology: Renal Physiology 1 1-10, 2004
View abstract

Mobasheri A; Trujillo E; Bell S; Carter SD; Clegg PD; Martin-Vasallo P; Marples DDR Aquaporin water channels AQP1 and AQP3, are expressed in equine articular chondrocytes The Veterinary Journal 168 143-150, 2004
DOI:10.1016/j.tvjl.2003.08.001
View abstract

Mobasheri A; Shakibaei M; Marples DDR Immunohistochemical localization of aquaporin 10 in the apical membranes of the human ileum: a potential pathway for luminal water and small solute absorption Histochemistry and Cell Biology 121 463-471, 2004
DOI:10.1007/s00418-004-0657-1
View abstract

Christensen BM; Marples DDR; Kim YH; Wang WD; Frokiaer J; Nielsen S Changes in cellular composition of kidney collecting duct cells in rats with lithium-induced NDI American Journal of Physiology: Cell Physiology 286 C952-C964, 2004
View abstract

Trujillo E; Gonzalez T; Marin R; Martin-Vasallo P; Marples DDR; Mobasheri A Human articular chondrocytes, synoviocytes and synovial microvessels express aquaporin water channels; upregulation of AQP1 in rheumatoid arthritis Histology and Histopathology 19 435-444, 2004
View abstract

Mobasheri A; Marples DDR Expression of the AQP-1 water channel in normal human tissues: a semiquantitative study using tissue microarray technology American Journal of Physiology: Cell Physiology 286 C529-C537, 2004
View abstract

Baggaley EM; Marples D Prostagla E-2 and I-2 cause AQP2 shuttling in isolated rat inner medullary collecting ducts, 2003

Marples D; Chipperfield S Angiotensin II causes shuttling of AQP2 water channels in rat inner medullary collecting ducts, 2003

Christensen BM; Marples DDR; Kim YH; Wang W; Frokiaer J; Nielsen S Changes in cellular composition of kidney collecting duct in rats with lithium-induced NDI American Journal of Physiology: Cell Physiology -, 2003

Mobasheri A; Marples DDR Expression of the aquaporin 1 (AQP1) water channel in normal human tissues: a semi-quantitative study using tissue microarray technology American Journal of Physiology: Cell Physiology -, 2003

Shaw S; Marples D A rat kidney tubule suspension for the study of vasopressin-induced shuttling of AQP2 water channels. Am J Physiol Renal Physiol 283 F1160-F1166, 2002
DOI:10.1152/ajprenal.00207.2002
View abstract

Shaw S; Marples D Dynein is important in vasopressin-induced shuttling of AQP2 water channels. J AM SOC NEPHROL 13 480A-480A, 2002

Shaw S; Marples D Protein kinase C modulates the response to vasopressin in rat inner medullary collecting ducts. J AM SOC NEPHROL 13 481A-481A, 2002

Trujillo-Martin E; Marin R; Masrani B; Marples D; Martin-Vasallo P; Gonzalez T; Mobasheri A Aquaporin water channels in porcine and human articular cartilage: Differential expression in pathologies of human cartilage., 2002

Christensen BM; Marples D; Wang W; Frokiaer J; Nielsen S Decreased fraction of principal cells in parallel with increased fraction of intercalated cells in rats with lithium-induced NDI. J AM SOC NEPHROL 13 270A-270A, 2002

Marples D; Shaw S N-ethyl maleimide causes AQP2 trafficking by direct activation of protein kinase A. J AM SOC NEPHROL 13 481A-481A, 2002

Mobasheri A; Trujillo E; Martin-Vasallo P; Marples D Aquaporins 1 and 3 are expressed in porcine articular chondrocytes J PHYSIOL-LONDON 539 14P-14P, 2002

Nielsen S; Frokiaer J; Marples DDR; Kwon TH; Agre P; Knepper MA Aquaporins in the kidney: From molecules to medicine. Physiological Reviews 82 205-244, 2002

Dobrzynski H; Marples DDR; Musa H; Yamanushi TT; Henderson Z; Takagishi Y; Honjo H; Kodama I; Boyett MR Distribution of the muscarinic K+ channel proteins Kir3.1 and Kir3.4 in the ventricle, atrium, and sinoatrial node of heart J HISTOCHEM CYTOCHEM 49 1221-1234, 2001

Shaw S; Marples D N-ethylmaleimide induces shuttling of aquaporin-2 water channels in a rat kidney tubule suspension, 2001

Li CL; Wang WD; Kwon TH; Isikay L; Wen JG; Marples D; Djurhuus JC; Stockwell A; Knepper MA; Nielsen S Downregulation of AQP1,-2, and-3 after ureteral obstruction is associated with a long-term urine-concentrating defect AM J PHYSIOL-RENAL 281 F163-F171, 2001

Nejsum LN; Kwon TH; Marples D; Flyvbjerg A; Knepper MA; Frokiaer J; Nielsen S Compensatory increase in AQP2, p-AQP2, and AQP3 expression in rats with diabetes mellitus AM J PHYSIOL-RENAL 280 F715-F726, 2001

Marples DDR Aquaporins: Roles in renal function and peritoneal dialysis Peritoneal Dialysis International 21 212-218, 2001
View abstract

Nielsen S; Kwon TH; Nager H; Knepper MA; Marples D; Frokiaer J Pathophysiology of renal aquaporins CURR TOP MEMBR 51 155-183, 2001

Nielsen S; Kwon T-H; Hager H; Knepper MA; Marples D; Frøkiaer J Chapter 4 Pathophysiology of renal aquaporins Current Topics in Membranes 51 155-183, 2001

Dobrzynski H; Rothery SM; Marples DDR; Coppen SR; Takagishi Y; Honjo H; Tamkun MM; Henderson Z; Kodama I; Boyett MR Presence of the Kv1.5 K(+) channel in the sinoatrial node. Journal of Histochemistry and Cytochemistry 48 769-780, 2000

Kwan TH; Laursen UH; Maunsbach AB; Marples DDR Altered expression of renal AQPs and Na+ transporters in rats with Lithium-induced NDI American Journal of Physiology 279 552-564, 2000

Frokiaer J; Marples DDR; Valtin H; Morris JF; Knepper MA; Neilsen S Low aquaporin-2 levels in polyuric DI +/+ severe mice with constitutively high cAMP-phosphodiesterase activity. Journal of Physiology 276 179-190, 1999

Marples DDR; Christensen BM; Frokiaer J; Knepper MA; Neilsen S Dehydration reverses vasopressin antagonist-induced diuresis and aquaporin-2 downregulation in rats. Journal of Physiology 275 400-409, 1998

Christensen BM; Marples DDR; Jenson UB; Frokiaer J; Sheikh-Hamad D; Knepper MA; Neilsen S Acute effects of vasopressin V2-receptor antagonist on kidney AQP2 expression and subcellular distribution. American Journal of Physiology 275 285-297, 1998

Marples DDR; Schroer TA; Ahrens N; Taylor A; Knepper MA; Neilsen S Dynein and dynactin colocalize with AQP2 water channels in intracellular vesicles from kidney collecting duct. Journal of Physiology 274 384-394, 1998

Nielsen S; Terris J; Andersen D; Ecelbarger C; Frokiaer J; Jonassen T; Marples DDR; Knepper MA; Petersen JS Congestive heart failure in rats is associated with increased expression and targeting of aquaporin-2 water channel in collecting duct Proceedings of the National Academy of Sciences of the United States of America 94 5450-5455, 1997

Frokiaer J; Dorup J; Knepper MA; Marples DDR Hypokalemia-induced downregulation of Aquaprin-2 water channel expression in rat kidney medulla and cortex Journal of Clinical Investigation 97 1960-1968, 1996