Faculty of Biological Sciences

Dr Arnout Kalverda

MSc, Netherlands; PhD, Leiden, Netherlands.
NMR Facilities Manager
School of Molecular and Cellular Biology

Contact:  Miall 4.21a, +44(0) 113 34 37997, email address for  

Hewitt SH, Filby MH, Hayes E, Kuhn LT, Kalverda AP, Webb ME, Wilson AJ Protein Surface Mimetics: Understanding How Ruthenium Tris(Bipyridines) Interact with Proteins ChemBioChem 18 223-231, 2017
DOI:10.1002/cbic.201600552
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Karamanos TK, Pashley CL, Kalverda AP, Thompson GS, Mayzel M, Orekhov VY, Radford SE A Population Shift between Sparsely Populated Folding Intermediates Determines Amyloidogenicity Journal of the American Chemical Society 138 6271-6280, 2016
DOI:10.1021/jacs.6b02464
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Tomlinson JH, Thompson GS, Kalverda AP, Zhuravleva A, O'Neill AJ A target-protection mechanism of antibiotic resistance at atomic resolution: Insights into FusB-type fusidic acid resistance Scientific Reports 6 -, 2016
DOI:10.1038/srep19524
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Hiruma-Shimizu K, Shimizu H, Thompson GS, Kalverda AP, Patching SG Deuterated detergents for structural and functional studies of membrane proteins: Properties, chemical synthesis and applications Molecular Membrane Biology 32 139-155, 2015
DOI:10.3109/09687688.2015.1125536
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Karamanos TK, Kalverda AP, Thompson GS, Radford SE Mechanisms of amyloid formation revealed by solution NMR Progress in Nuclear Magnetic Resonance Spectroscopy 88-89 86-104, 2015
DOI:10.1016/j.pnmrs.2015.05.002
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Monteiro DCF, Patel V, Bartlett CP, Nozaki S, Grant TD, Gowdy JA, Thompson GS, Kalverda AP, Snell EH, Niki H, Pearson AR, Webb ME The structure of the PanD/PanZ protein complex reveals negative feedback regulation of pantothenate biosynthesis by coenzyme a Chemistry and Biology 22 492-503, 2015
DOI:10.1016/j.chembiol.2015.03.017
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Czajlik A, Thompson GS, Khan GN, Kalverda AP, Homans SW, Trinick J ¹H, ¹⁵N, and ¹³C backbone chemical shift assignment of titin domains A59-A60 and A60 alone Biomolecular NMR assignments 8 429-433, 2014
DOI:10.1007/s12104-013-9532-0
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Czajlik A, Thompson GS, Khan GN, Kalverda AP, Homans SW, Trinick J ¹H, ¹⁵N, and ¹³C backbone chemical shift assignment of titin domains A59-A60 and A60 alone. Biomol NMR Assign 8 429-433, 2014
DOI:10.1007/s12104-013-9532-0
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Sarell CJ, Karamanos TK, White SJ, Bunka DH, Kalverda AP, Thompson GS, Barker AM, Stockley PG, Radford SE Distinguishing closely related amyloid precursors using an RNA aptamer. J Biol Chem 289 26859-26871, 2014
DOI:10.1074/jbc.M114.595066
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Karamanos TK, Kalverda AP, Thompson GS, Radford SE Visualization of transient protein-protein interactions that promote or inhibit amyloid assembly. Mol Cell 55 214-226, 2014
DOI:10.1016/j.molcel.2014.05.026
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Kalverda AP, Gowdy J, Thompson GS, Homans SW, Henderson PJ, Patching SG TROSY NMR with a 52 kDa sugar transport protein and the binding of a small-molecule inhibitor. Mol Membr Biol 31 131-140, 2014
DOI:10.3109/09687688.2014.911980
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Foster TL, Thompson GS, Kalverda AP, Kankanala J, Bentham M, Wetherill LF, Thompson J, Barker AM, Clarke D, Noerenberg M, Pearson AR, Rowlands, D. J., Homans SW, Harris M, Foster R, Griffin S Structure-guided design affirms inhibitors of hepatitis C virus p7 as a viable class of antivirals targeting virion release Hepatology 59 408-422, 2014
DOI:10.1002/hep.26685
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Fisher M, Basak R, Kalverda AP, Fishwick CW, Turnbull WB, Nelson A Discovery of novel FabF ligands inspired by platensimycin by integrating structure-based design with diversity-oriented synthetic accessibility. Org Biomol Chem 12 486-494, 2014
DOI:10.1039/c3ob41975d
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Hiruma-Shimizu K, Kalverda AP, Henderson PJF, Homans SW, Patching SG Synthesis of uniformly deuterated n-dodecyl-β -d-maltoside (d39 -DDM) for solubilization of membrane proteins in TROSY NMR experiments Journal of Labelled Compounds and Radiopharmaceuticals 57 737-743, 2014
DOI:10.1002/jlcr.3249
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Czajlik A, Thompson GS, Khan GN, Kalverda AP, Homans SW, Trinick J ¹H, ¹⁵N and ¹³C backbone chemical shift assignment of the titin A67-A68 domain tandem. Biomol NMR Assign 6 39-41, 2012
DOI:10.1007/s12104-011-9321-6
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Pashley CL, Morgan GJ, Kalverda AP, Thompson GS, Kleanthous C, Radford SE Conformational properties of the unfolded state of Im7 in nondenaturing conditions. J Mol Biol 416 300-318, 2012
DOI:10.1016/j.jmb.2011.12.041
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Eichner T, Kalverda AP, Thompson GS, Homans SW, Radford SE Conformational conversion during amyloid formation at atomic resolution. Mol Cell 41 161-172, 2011
DOI:10.1016/j.molcel.2010.11.028
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Czajlik A, Thompson GS, Khan GN, Kalverda AP, Homans SW, Trinick J 1H, 15N and 13C backbone chemical shift assignment of the titin A67-A68 domain tandem Biomolecular NMR Assignments 1-3, 2011

Houssen WE, Wright SH, Kalverda AP, Thompson GS, Kelly SM, Jaspars M Solution structure of the leader sequence of the patellamide precursor peptide, PatE1-34. Chembiochem 11 1867-1873, 2010
DOI:10.1002/cbic.201000305
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Kalverda AP, Thompson GS, Vogel A, Schroder M, Bowie AG, Khan AR, Homans SW Poxvirus K7 Protein Adopts a Bcl-2 Fold: Biochemical Mapping of Its Interactions with Human DEAD Box RNA Helicase DDX3 J MOL BIOL 385 843-853, 2009
DOI:10.1016/j.jmb.2008.09.048

Stöckmann H, Bronowska A, Syme NR, Thompson GS, Kalverda AP, Warriner SL, Homans SW Residual ligand entropy in the binding of p-substituted benzenesulfonamide ligands to bovine carbonic anhydrase II. J Am Chem Soc 130 12420-12426, 2008
DOI:10.1021/ja803755m
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Burnley BT, Kalverda AP, Paisey SJ, Berry A, Homans SW Hadamard NMR spectroscopy for relaxation measurements of large (>35 kDa) proteins J BIOMOL NMR 39 239-245, 2007
DOI:10.1007/s10858-007-9192-4

Knight PJ, Thirumurugan K, Xu Y, Wang F, Kalverda AP, Stafford III WF, Sellers JR, Peckham M The Predicted Coiled-coil Domain of Myosin 10 Forms a Novel Elongated Domain That Lengthens the Head Journal of Biological Chemistry 280 34702-34708, 2005
DOI:10.1074/jbc.M504887200

Plattt GW, McParland VJ, Kalverda AP, Homans SW, Radford SE Dynamics in the unfolded state of beta(2)-microglobulin studied by NMR J MOL BIOL 346 279-294, 2005
DOI:10.1016/j,jmb.2004.11.035

Peckham M, Kalverda AP, Stafford WF, Knight PJ A stable, single stranded alpha-helix in unconventional myosins, 2005

Kalverda AP, Thompson GS, Turnbull WB The importance of protein structural dynamics and the contribution of NMR spectroscopy In Encyclopedia of Genetics, Genomics, Proteomics and Bioinformatics , 2005

Gorski SA, Le Duff CS, Capaldi AP, Kalverda AP, Beddard GS, Moore GR, Radford SE Equilibrium hydrogen exchange reveals extensive hydrogen bonded secondary structure in the on-pathway intermediate of Im7 J MOL BIOL 337 183-193, 2004
DOI:10.1016/j.jmb.2004.01.004

Bingham RJ, Findlay JBC, Hsieh SY, Kalverda AP, Kjeliberg A, Perazzolo C, Phillips SEV, Seshadri K, Trinh CH, Turnbull WB, Bodenhausen G, Homans SW Thermodynamics of binding of 2-methoxy-3-isopropylpyrazine and 2-methoxy-3-isobutylpyrazine to the major urinary protein J AM CHEM SOC 126 1675-1681, 2004
DOI:10.1021/ja038461i

Daranas AH, Kalverda AP, Chiovitti A, Homans SW Backbone resonance assignment of the L-arabinose binding protein in complex with D-galactose. J Biomol NMR 28 191-192, 2004
DOI:10.1023/B:JNMR.0000013838.08280.27

Yung A, Turnbull WB, Kalverda AP, Thompson GS, Homans SW, Kitov P, Bundle DR Large-scale millisecond intersubunit dynamics in the B subunit homopentamer of the toxin derived from Escherichia coli O157. J Am Chem Soc 125 13058-13062, 2003
DOI:10.1021/ja0367288
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Chaykovski MM, Bae LC, Cheng MC, Murray JH, Tortolani KE, Zhang R, Seshadri K, Findlay JBC, Hsieh SY, Homans SW, Kalverda AP, Brown JM Methyl side-chain dynamics in proteins using selective enrichment with a single isotopomer Journal of the American Chemical Society 125 15767-15771, 2003
DOI:10.1021/ja0368608
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Trinh CH, Smith DP, Kalverda AP, Phillips SEV, Radford SE Crystal structure of monomeric humanß-2-microglobulin reveals clues to its amyloidogenic properties Proceedings of the National Academy of Sciences of the United States of America 99 9771-9776, 2002
DOI:10.1073/pnas.152337399

McParland VJ, Kalverda AP, Homans SW, Radford SE Structural properties of an amyloid precursor of&beta;<sub>2</sub>-microglobulin Nature Structural Biology 9 326-331, 2002
DOI:10.1038/nsb791

Gell C, Brockwell DJ, Beddard GS, Radford SE, Kalverda AP, Smith DA Accurate use of single molecule fluorescence correlation spectroscopy to determine molecular diffusion times SINGLE MOL 2 177-181, 2001

Hilcenko C, Berry A, Kalverda AP, Homans SW Multi-dimensional nuclear magnetic resonance studies of the dynamics and mechanism of class II fructose-1,6-bisphosphate aldolase Biophysical Journal 80 1666-1666, 2001

Jones S, Reader JS, Healy M, Capaldi AP, Ashcroft AE, Kalverda AP, Smith DA, Radford SE Partially unfolded species populated during equilibrium denaturation of the beta-sheet protein Y74W apo-pseudoazurin. Biochemistry 39 5672-5682, 2000
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McParland VJ, Kad NM, Kalverda AP, Brown A, Kirwin-Jones P, Hunter MG, Sunde M, Radford SE Partially unfolded states of beta(2)-microglobulin and amyloid formation in vitro ChemBioChem 39 8735-8746, 2000

Kalverda AP, Ubbink M, Gilardi G, Wijmenga SS, Crawford A, Jeuken LJC, Canters GW Backbone dynamics of azurin in solution: Slow conformational change associated with deprotonation of histidine 35 Biochemistry 38 12690-12697, 1999
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