Prof Peter Henderson

Faculty of Biological Sciences

MA, ScD, Cambridge; BSc, PhD 1968, Bristol
Professor of Biochemistry and Molecular Biology
School of Biomedical Sciences

Background: Postdoctoral research at The Enzyme Institute, University of Wisconsin, Madison, USA 1968-71; Biochemistry Department, Leicester University 1971-73. Lecturer in Biochemistry, University of Leicester, 1973-75 and University of Cambridge, 1975-90. Royal Society Visiting Professor, Jichi Medical School, Japan, 1981-82. Reader in the Molecular Biology of Membranes, University of Cambridge, 1990-92. Professor of Biochemistry and Molecular Biology, University of Leeds, 1992-present. Canadian Commonwealth Research Fellow, University of Guelph, Ontario, Canada, 1993. Burroughs Wellcome Visiting Professor of Microbiology, University of New Mexico, Albuquerque, 1996.

Contact: Astbury 6.108b | +44(0) 113 34 33175 | email address for Prof Peter Henderson

You can read more about Prof Henderson's interests here:
www.astbury.leeds.ac.uk/People/staffpage.php?StaffID=PJFH

Modules taught

BIOC322*/BIOL3210/BMSC323* - ATU 23 - Membrane proteins
BIOC1301 - Intro Inte Biochem Mol Process

Centre membership:

Leader of the Peter Henderson group

Mr David Sharples (Fermenter Technician)

Prof Peter Henderson (Group Leader, Professor of Biochemistry and Molecular Biology)

The molecular mechanisms, structure and regulation of membrane transport proteins in microbes and man

Postgraduates

Anna Polyakova

Shimamura T; Weyand S; Beckstein O; Rutherford NG; Hadden JM; Sharples D; Sansom MSP; Iwata S; Henderson PJF; Cameron AD Molecular Basis of Alternating Access Membrane Transport by the Sodium-Hydantoin Transporter Mhp1 SCIENCE 328 470-473, 2010
DOI:10.1126/science.1186303

Psakis G; Saidijam M; Shibayama K; Polaczek J; Bettaney KE; Baldwin JM; Baldwin SA; Hope R; Essen LO; Essenberg RC; Henderson PJF The sodium-dependent d-glucose transport protein of Helicobacter pylori MOL MICROBIOL 71 391-403, 2009
DOI:10.1111/j.1365-2958.2008.06535.x

Patching SG; Henderson PJF; Herbert RB; Middleton DA Solid-state NMR Spectroscopy detects interactions between tryptophan residues of the E-coli sugar transporter GaIP and the alpha-anomer of the D-glucose substrate J AM CHEM SOC 130 1236-1244, 2008
DOI:10.1021/ja075584k

Weyand S; Shimamura T; Yajima S; Suzuki S; Mirza O; Krusong K; Carpenter EP; Rutherford NG; Hadden JM; O'Reilly J; Ma P; Saidijam M; Patching SG; Hope RJ; Norbertczak HT; Roach PCJ; Iwata S; Henderson PJF; Cameron AD Structure and Molecular Mechanism of a Nucleobase-Cation-Symport-1 Family Transporter SCIENCE 322 709-713, 2008
DOI:10.1126/science.1164440

Patching SG; Jackson S; Simmons KJ; Ma P; Baldwin SA; Johnson P; Fishwick CWG; Henderson PJF Isotope chemistry for investigating ligand binding and transport by Mhp1 and its homologues JOURNAL OF LABELLED COMPOUNDS&RADIOPHARMACEUTICALS 55 132-132, 2012

Patching SG; Middleton DA; Kalverda A; Gowdy J; Baldwin SA; Phillips-Jones MK; Levitt MH; Homans SW; Henderson PJF NMR methods for structure-function investigation of membrane proteins and their ligands JOURNAL OF LABELLED COMPOUNDS&RADIOPHARMACEUTICALS 55 132-133, 2012

Simm R; Voros A; Ekman JV; Sodring M; Nes I; Kroeger JK; Saidijam M; Bettaney KE; Henderson PJF; Salkinoja-Salonen M; Kolsto A-B BC4707 Is a Major Facilitator Superfamily Multidrug Resistance Transport Protein from Bacillus cereus Implicated in Fluoroquinolone Tolerance PLOS ONE 7 -, 2012
DOI:10.1371/journal.pone.0036720

Henderson PJF; Baldwin SA Structural biology: Bundles of insights into sugar transporters Nature 490 348-350, 2012
DOI:10.1038/490348a

Weyand S; Shimamura T; Beckstein O; Sansom MSP; Iwata S; Henderson PJF; Cameron AD The alternating access mechanism of transport as observed in the sodium-hydantoin transporter Mhp1, 2011
DOI:10.1107/S0909049510032449

Bill RM; Henderson PJF; Iwata S; Kunji ERS; Michel H; Neutze R; Newstead S; Poolman B; Tate CG; Vogel H Overcoming barriers to membrane protein structure determination NAT BIOTECHNOL 29 335-340, 2011
DOI:10.1038/nbt.1833

Robichaud T; Appleyard AN; Herbert RB; Henderson PJF; Carruthers A Determinants of Ligand Binding Affinity and Cooperativity at the GLUT1 Endofacial Site BIOCHEMISTRY-US 50 3137-3148, 2011
DOI:10.1021/bi1020327

Abrahams J-P; Apweiler R; Balling R; Bertero MG; Bujnicki JM; Chayen NE; Chène P; Corthals GL; Dylag T; Jehenson P; Kokalj SJ; Förster F; Heck AJR; Henderson PJF; Herwig R; Laue E; Legrain P; Martens L; Migliorini C; Musacchio A; Podobnik M; Schertler GFX; Schreiber G; Sixma TK; Smit AB; Stuart D; Svergun DI; Taussig MJ "4D Biology for health and disease" workshop report New Biotechnology 28 291-293, 2011
DOI:10.1016/j.nbt.2010.10.003
View abstract

Jones LN; Baldwin SA; Henderson PJF; Ashcroft AE Defining topological features of membrane proteins by nanoelectrospray ionisation mass spectrometry RAPID COMMUN MASS SP 24 276-284, 2010
DOI:10.1002/rcm.4387

Miller K; Dunsmore CJ; Leeds JA; Patching SG; Sachdeva M; Blake KL; Stubbings WJ; Simmons KJ; Henderson PJF; De Los Angeles J; Fishwick CWG; Chopra I Benzothioxalone derivatives as novel inhibitors of UDP-N-acetylglucosamine enolpyruvyl transferases (MurA and MurZ) J ANTIMICROB CHEMOTH 65 2566-2573, 2010
DOI:10.1093/jac/dkq349

Findlay HE; Rutherford NG; Henderson PJF; Booth PJ Unfolding free energy of a two-domain transmembrane sugar transport protein P NATL ACAD SCI USA 107 18451-18456, 2010
DOI:10.1073/pnas.1005729107

Jeuken LJC; Weiss SA; Daskalakis NN; Henderson PJF; Evans SD; Bushby RJ Novel electrodes for redox-active membrane enzymes ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY 240 -, 2010

Blake KL; O'Neill AJ; Mengin-Lecreulx D; Henderson PJF; Bostock JM; Dunsmore CJ; Simmons KJ; Fishwick CWG; Leeds JA; Chopra I The nature of Staphylococcus aureus MurA and MurZ and approaches for detection of peptidoglycan biosynthesis inhibitors MOL MICROBIOL 72 335-343, 2009
DOI:10.1111/j.1365-2958.2009.06648.x

Tanabe M; Szakonyi G; Brown KA; Henderson PJF; Nield J; Byrne B The multidrug resistance efflux complex, EmrAB from Escherichia coli forms a dimer in vitro BIOCHEM BIOPH RES CO 380 338-342, 2009
DOI:10.1016/j.bbrc.2009.01.081

Weiss SA; Bushby RJ; Evans SD; Henderson PJF; Jeuken LJC Characterization of cytochrome bo(3) activity in a native-like surface-tethered membrane BIOCHEM J 417 555-560, 2009
DOI:10.1042/BJ20081345

Dunsmore CJ; Miller K; Blake KL; Patching SG; Henderson PJF; Garnett JA; Stubbings WJ; Phillips SEV; Palestrant DJ; Angeles JDL; Leeds JA; Chopra I; Fishwick CWG 2-aminotetralones: Novel inhibitors of MurA and MurZ BIOORG MED CHEM LETT 18 1730-1734, 2008
DOI:10.1016/j.bmcl.2008.01.089

Middleton DA; Baldwin SA; Herbert RB; Henderson PJF; Patching SG Exploiting isotope labelling for solid-state NMR studies of drugs and their targets J LABELLED COMPD RAD 51 249-249, 2008

Postis VLG; Deacon SE; Roach PCJ; Wright GSA; Xia X; Ingram JC; Hadden JM; Henderson PJF; Phillips SEV; McPherson MJ; Baldwin SA A high-throughput assay of membrane protein stability MOL MEMBR BIOL 25 617-624, 2008
DOI:10.1080/09687680802530469

Rahman M; Patching SG; Ismat F; Henderson PJF; Herbert RB; Baldwin SA; McPherson MJ Probing metal ion substrate-binding to the E. coli ZitB exporter in native membranes by solid state NMR MOL MEMBR BIOL 25 683-690, 2008
DOI:10.1080/09687680802495267

Jeuken LJC; Weiss SA; Henderson PJF; Evans SD; Bushby RJ Impedance Spectroscopy of Bacterial Membranes: Coenzyme-Q Diffusion in a Finite Diffusion Layer ANAL CHEM 80 9084-9090, 2008
DOI:10.1021/ac8015856

Patching SG; Psakis G; Baldwin SA; Baldwin J; Henderson PJF; Middleton DA Relative substrate affinities of wild-type and mutant forms of the Escherichia coli sugar transporter GalP determined by solid-state NMR MOL MEMBR BIOL 25 474-484, 2008
DOI:10.1080/09687680802371963

Ma PK; Yuille HM; Blessie V; Gohring N; Igloi Z; Nishiguchi K; Nakayama J; Henderson PJF; Phillips-Jones MK Expression, purification and activities of the entire family of intact membrane sensor kinases from Enterococcus faecalis MOL MEMBR BIOL 25 449-473, 2008
DOI:10.1080/09687680802359885

Xia XB; Postis VLG; Rahman M; Wright GSA; Roach PCJ; Deacon SE; Ingram JC; Henderson PJF; Findlay JBC; Phillips SEV; McPherson MJ; Baldwin SA Investigation of the structure and function of a Shewanella oneidensis arsenical-resistance family transporter MOL MEMBR BIOL 25 691-705, 2008
DOI:10.1080/09687680802535930

Shimamura T; Yajima S; Suzuki S; Rutherford NG; O'Reilly J; Henderson PJF; Iwata S Crystallization of the hydantoin transporter Mhp1 from Microbacterium liquefaciens ACTA CRYSTALLOGR F 64 1172-1174, 2008
DOI:10.1107/S1744309108036920

Weiss SA; Evans SD; Bushby R; Henderson PJF; Jeuken LJC Characterisation of ubiquinol oxidase activity in a native-like membrane using voltammetry, 2008
DOI:10.1016/j.bbabio.2008.05.264

Deacon SE; Roach PCJ; Postis VLG; Wright GSA; Xia XB; Phillips SEV; Knox JP; Henderson PJF; McPherson MJ; Baldwin SA Reliable scale-up of membrane protein over-expression by bacterial auto-induction: From microwell plates to pilot scale fermentations MOL MEMBR BIOL 25 588-598, 2008
DOI:10.1080/09687680802511774

Reig N; del Rio C; Casagrande F; Ratera M; Gelpi JL; Torrents D; Henderson PJF; Xie H; Baldwin SA; Zorzano A; Fotiadis D; Palacin M Functional and structural characterization of the first prokaryotic member of the L-amino acid transporter (LAT) family - A model for APC transporters J BIOL CHEM 282 13270-13281, 2007
DOI:10.1074/jbc.M610695200

Szakonyi G; Leng D; Ma P; Bettaney KE; Saidijam M; Ward A; Zibaei S; Gardiner AT; Cogdell RJ; Butaye P; Kolsto AB; O'Reilly J; Hope RJ; Rutherford NG; Hoyle CJ; Henderson PJF A genomic strategy for cloning, expressing and purifying efflux proteins of the major facilitator superfamily J ANTIMICROB CHEMOTH 59 1265-1270, 2007
DOI:10.1093/jac/dkm036

Gutmann DAP; Mizohata E; Newstead S; Ferrandon S; Postis V; Xia XB; Henderson PJF; Van Veen HW; Byrne B A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay (vol 16, pg 1422, 2007) PROTEIN SCI 16 2775-2775, 2007

Gutmann DAP; Mizohata E; Newstead S; Ferrandon S; Henderson PJF; Van Veen HW; Byrne B A high-throughput method for membrane protein solubility screening: The ultracentrifugation dispersity sedimentation assay PROTEIN SCI 16 1422-1428, 2007
DOI:10.1110/ps.072759907

Shibayama K; Wachino J; Arakawa Y; Saidijam M; Rutherford NG; Henderson PJF Metabolism of glutamine and glutathione via gamma-glutamyltranspeptidase and glutamate transport in Helicobacter pylori: possible significance in the pathophysiology of the organism MOL MICROBIOL 64 396-406, 2007
DOI:10.1111/j.1365-2958.2007.05661.x

Saidijam M; Benedetti G; Ren QH; Xu ZQ; Hoyle CJ; Palmer SL; Ward A; Bettaney KE; Szakonyi G; Meuller J; Morrison S; Pos MK; Butaye P; Walravens K; Langton K; Herbert RB; Skurray RA; Paulsen IT; O'Reilly J; Rutherford NG; Brown MH; Bill RM; Henderson PJF Microbial drug efflux proteins of the major facilitator superfamily CURR DRUG TARGETS 7 793-811, 2006

Jeuken LJC; Connell SD; Henderson PJF; Gennis RB; Evans SD; Bushby RJ Redox enzymes in tethered membranes J AM CHEM SOC 128 1711-1716, 2006
DOI:10.1021/ja056972u

Potter CA; Jeong EL; Williamson MP; Henderson PJF; Phillips-Jones MK Redox-responsive in vitro modulation of the signalling state of the isolated PrrB sensor kinase of Rhodobacter sphaeroides NCIB 8253 FEBS LETT 580 3206-3210, 2006
DOI:10.1016/j.febslet.2006.04.079

Spencelayh MJ; Cheng YL; Bushby RJ; Bugg TDH; Li JJ; Henderson PLF; O'Reilly J; Evans SD Antibiotic action and peptidoglycan formation on tethered lipid bilayer membranes ANGEW CHEM INT EDIT 45 2111-2116, 2006
DOI:10.1002/anie.200504035

Suzuki S; Henderson PJF The hydantoin transport protein from Microbacterium liquefaciens J BACTERIOL 188 3329-3336, 2006
DOI:10.1128/JB.188.9.3329-3336.2006

Liang WJ; Wilson KJ; Xie H; Knol J; Suzuki S; Rutherford N; Henderson PJF; Jefferson RA The gusBC Genes of Escherichia coli Encode a Glucuronide Transport System Journal of Bacteriology 187 2377-2385, 2005
DOI:10.1128/JB.187.7.2377-2385.2005
View abstract

Langton KP; Henderson PJF; Herbert RB Antibiotic resistance: multidrug efflux proteins, a common transport mechanism? NAT PROD REP 22 439-451, 2005
DOI:10.1039/b413734p

Patching SG; Baldwin SA; Baldwin AD; Young JD; Gallagher MP; Henderson PJF; Herbert RB The nucleoside transport proteins, NupC and NupG, from Escherichia coli: specific structural motifs necessary for the binding of ligands Organic&Biomolecular Chemistry 3 462-470, 2005
DOI:10.1039/b414739a
View abstract

A series of 46 natural nucleosides and analogues (mainly adenosine-based) were tested as inhibitors of [U-C-14]uridine uptake by the concentrative, H+-linked nucleoside transport proteins NupC and NupG from Escherichia coli. The two evolutionarily unrelated transporters showed similar but distinct patterns of inhibition, revealing differing selectivities for the different nucleosides and their analogues. Binding of nucleosides to NupG required the presence of hydroxyl groups at each of the C-3' and C-5' positions of ribose, while binding to NupC required only the C-3' hydroxyl substituent. The greater importance of the ribose moiety for binding to NupG is consistent with the evolutionary relationship between this protein and the oligosaccharide: H+ symporter (OHS) subfamily of the major facilitator superfamily (MFS) of transporters. For both proteins the natural alpha-configuration at C-3' and the natural beta-configuration at C-1' was mandatory for ligand binding. N-7 in the imidazole ring of adenosine and the amino group at C-6 were found not to be important for binding and both transporters showed exibility for substitution at C-6/N-6; one or both of N-1 and N-3 were important for adenosine analogue binding to NupC but significantly less so for binding to NupG. From the different effects of 8-bromoadenosine on the two transporters it appears that adenosine selectively binds to NupC in an anti-rather than a syn-conformation, whereas NupG is less prescriptive. The pattern of inhibition of NupC by differing nucleoside analogues confirmed the functional relationship of the bacterial transporter to members of the human concentrative nucleoside transporter (CNT) family and reaffirmed the use of the bacterial protein as an experimental model for these physiologically and clinically important mammalian proteins. The specificity data for NupG have been used to develop a homology model of the protein's binding site, based on the X-ray crystallographic structure of the disaccharide transporter LacY from E. coli. We have also developed an efficient general protocol for the synthesis of adenosine and three of its analogues, which is illustrated by the synthesis of [1'-C-13]adenosine.
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Saidijam M; Bettaney KE; Szakonyi G; Psakis G; Shibayama K; Suzuki S; Clough J; Blessie V; Abu-Bakr A; Baumberg S; Meuller J; Hoyle C; Palmer SL; Butaye P; Walravens K; Patching SG; O'Reilly J; Rutherford N; Bill RM; Roper DI; Phillips-Jones MK; Henderson PJF Active membrane transport and receptor proteins from bacteria Biochemical Society Transactions 33 867-872, 2005
DOI:10.1042/BST0330867
View abstract

Jeuken LJC; Connell SDA; Nurnabi M; O'Reilly J; Henderson PJF; Evans SD; Bushby RJ Direct electrochemical interaction between a modified gold electrode and a bacterial membrane extract Langmuir 21 1481-1488, 2005
DOI:10.1021/la047732f
View abstract

Patching SG; Brough AR; Herbert RB; Rajakarier AR; Henderson PJF; Middleton DA Substrate Affinities for Membrane Transport Proteins Determined by¹³C Cross-Polarization Magic-Angle Spinning Nuclear Magnetic Resonance Spectroscopy Journal of the American Chemical Society 126 3072-3080, 2004
DOI:10.1021/ja037163i

Patching SG; Herbert RB; O'Reilly J; Brough AR; Henderson PJF Low (13)C-background for NMR-based studies of ligand binding using (13)C-depleted glucose as carbon source for microbial growth: (13)C-Labeled glucose and (13)C-forskolin binding to the galactose-H(+) symport protein GalP in Escherichia coli Journal of the American Chemical Society 126 86-87, 2004
DOI:10.1021/ja038275c
View abstract

Xie H; Patching SG; Gallagher MP; Litherland GJ; Brough AR; Venter H; Yao SYM; Ng AML; Young JD; Herbert RB; Henderson PJF; Baldwin SA Purification and properties of the Escherichia coli nucleoside transporter NupG, a paradigm for a major facilitator transporter sub-family Molecular Membrane Biology 21 323-336, 2004
DOI:10.1080/09687860400003941
View abstract

NupG from Escherichia coli is the archetype of a family of nucleoside transporters found in several eubacterial groups and has distant homologues in eukaryotes, including man. To facilitate investigation of its molecular mechanism, we developed methods for expressing an oligohistidine-tagged form of NupG both at high levels (>20% of the inner membrane protein) in E. coli and in Xenopus laevis oocytes. In E. coli recombinant NupG transported purine (adenosine) and pyrimidine (uridine) nucleosides with apparent Km values of¡20-30 ¥ìM and transport was energized primarily by the membrane potential component of the proton motive force. Competition experiments in E. coli and measurements of uptake in oocytes confirmed that NupG was a broad-specificity transporter of purine and pyrimidine nucleosides. Importantly, using high-level expression in E. coli and magic-angle spinning cross-polarization solid-state nuclear magnetic resonance, we have for the first time been able directly to measure the binding of the permeant ([1¡Ç-13C]uridine) to the protein and to assess its relative mobility within the binding site, under non-energized conditions. Purification of over-expressed NupG to near homogeneity by metal chelate affinity chromatography, with retention of transport function in reconstitution assays, was also achieved. Fourier transform infrared and circular dichroism spectroscopy provided further evidence that the purified protein retained its 3D conformation and was predominantly ¥á-helical in nature, consistent with a proposed structure containing 12 transmembrane helices. These findings open the way to elucidating the molecular mechanism of transport in this key family of membrane transporters.
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Loewen SK; Yao SYM; Slugoski MD; Mohabir NN; Turner RJ; Mackey JR; Weiner JH; Gallagher MP; Henderson PJF; Baldwin SA; Cass CE; Young JD Transport of physiological nucleosides and anti-viral and anti-neoplastic nucleoside drugs by recombinant Molecular Membrane Biology 21 1-10, 2004
DOI:10.1080/0968768031000140836
Suzuki S; Yokozeki K; Henderson PJF A novel transporter protein, 2003
Morrison S; Ward A; Hoyle CJ; Henderson PJF Cloning, expression, purification and properties of a putative multidrug resistance efflux protein from Helicobacter pylori INT J ANTIMICROB AG 22 242-249, 2003
DOI:10.1016/S0924-8579(03)00222-X
Hubert AK; Henderson PJF; Marsh D Lipid-protein interactions in Escherichia coli membranes overexpressing the sugar-H+ symporter, GalP EPR of spin-labelled lipids Biochimica et Biophysica Acta 1611 243-248, 2003
Amaral A; Viveiros M; Henderson PJF Special issue - Efflux pumps and antibiotic resistance of microorganisms - Editorial International Journal of Antimicrobial Agents and Chemotherapy 22 176-176, 2003
Patching SG; Middleton DA; Henderson PJF; Herbert RB The economical synthesis of [2’-13C, 1,3-15N2] uridine; preliminary conformational studies by solid state NMR. Organic&Biomolecular Chemistry 1 2057-2062, 2003
DOI:10.1039/b301275a
View abstract

The synthesis of [2'-C-13, 1,3-N-15(2)] uridine 11 was achieved as follows. An epimeric mixture of D-[1-C-13] ribose 3 and D-[1-C-13] arabinose 4 was obtained in excellent yield by condensation of (KCN)-C-13 with D-erythrose 2 using a modification of the Kiliani-Fischer synthesis. Efficient separation of the two aldose epimers was pivotally achieved by a novel ion-exchange (Sm3+) chromatography method. D-[2-C-13] Ribose 5 was obtained from D-[1-C-13] arabinose 4 using a Ni(II) diamine complex (nickel chloride plus TEMED). Combination of these procedures in a general cycling manner can lead to the very efficient preparation of specifically labelled C-13-monosaccharides of particular chirality. N-15-labelling was introduced in the preparation of [2'- C-13, 1,3-N-15(2)] uridine 11 via [N-15(2)] urea. Cross polarisation magic angle spinning (CP-MAS) solid-state NMR experiments using rotational echo double resonance (REDOR) were carried out on crystals of the labelled uridine to show that the inter-atomic distance between C-2' and N-1 is closely similar to that calculated from X-ray crystallographic data. The REDOR method will be used now to determine the conformation of bound substrates in the bacterial nucleoside transporters NupC and NupG.
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Saidijam M; Psakis G; Clough JL; Meuller J; Suzuki S; Hoyle CJ; Palmer SL; Morrison SM; Pos MK; Essenberg RC; Maiden MCJ; Abu-Bakr A; Baumberg SG; Neyfakh AA; Griffith JK; Stark MJ; Ward A; O'Reilly J; Rutherford NG; Phillips-Jones MK; Henderson PJF Collection and characterisation of bacterial membrane proteins FEBS LETT 555 170-175, 2003
View abstract

A general strategy for the amplified expression in Escherichia coli of membrane transport and receptor proteins from other bacteria is described. As an illustration we report the cloning of the putative a-ketoglutarate membrane transport gene from the genome of Helicobacter pylori, overexpression of the protein tagged with RGS(His)(6) at the C-terminus, and its purification in mg quantities. The retention of structural and functional integrity was verified by circular dichroism spectroscopy and reconstitution of transport activity. This strategy for overexpression and purification is extended to additional membrane proteins from H. pylori and from other bacteria. (C) 2003 Published by Elsevier B.V. on behalf of the Federation of European Biochemical Societies.
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Ashcroft AE; Venter H; Keen JN; Henderson PJF; Herbert RB Molecular dissection of membrane transport proteins: mass spectrometry and sequence determination of the galactose-H⁺symport protein, GalP, of Escherichia coli and quantitative assay of the incorporation of [ring-2-¹³C]histidine.. Biochemical Journal 363 243-252, 2002
DOI:10.1042/0264-6021:3630243
Potter CA; Ward A; Laguri C; Williamson MP; Henderson PJF; Phillips-Jones MK Expression, Purification and Characterisation of Full-length Histidine Protein Kinase RegB from Rhodobacter sphaeroides Journal of Molecular Biology 320 201-213, 2002
DOI:10.1016/S0022-2836(02)00424-2
Marsh D; Henderson PJF Specific spin labelling of the sugar-H+ symporter, GalP, in cell membranes of Escherichia coli: site mobility and overall rotational diffusion of the protein BBA-BIOMEMBRANES 1510 464-473, 2001
Marsh D; Henderson PJF Specific spin labelling of the sugar-H+ symport protein, GalP, in cell membranes of Escherichia coli Biochimica et Biophysica Acta 1510 454-473, 2001
Ward A; Hoyle CJ; Palmer SA; O'Reilly J; Griffiths JK; Pos KM; Morrison S; Poolman B; Gwynne M; Henderson PJF Prokaryote multidrug efflux proteins of the major facilitator superfamily: amplified expression, purification and characterisation. Journal of Molecular Microbiology and Biotechnology 3 193-200, 2001
Hamilton SR; Yao SYM; Ingram JC; Hadden D; Ritzel MWL; Gallagher MP; Henderson PJF; Cass CE; Young JD; Baldwin SA Subcellular Distribution and Membrane Topology of the Mammalian Concentrative Na⁺-Nucleoside Cotransporter rCNT1 Journal of Biological Chemistry 276 27981-27988, 2001
DOI:10.1074/jbc.M100518200
McDonald TP; Henderson PJF Cysteine residues in the D-galactose-H+ symport protein of Escherichia coli: effects of mutagenesis on transport, reaction with N-ethylmaleimide and antibiotic binding BIOCHEM J 353 709-717, 2001
Appleyard A; Spooner PJR; Henderson PJF; Watts A; Herbert RB Direct observation of the binding of an inhibitor and its dosplacement of substrate in the galactose-H+ symport protein, GalP, in native membranes of Escherichia coli: a solid state NMR study. Biochimica et Biophysica Acta -, 2000
Herbert RB; Henderson PJF; Appleyard AN; Watts A; Spooner PJR Selective NMR observation of inhibitor and sugar binding to the galactose-H⁺symport protein GalP ofEscherichia coli Biochimica et Biophysica Acta 1509 55-64, 2000
Melvin F; McNeill A; Henderson PJF; Herbert RB The improved synthesis of beta-D-glucuronides using TEMPO and t-butyl hypochlorite Tetrahedron Letters 40 1201-1202, 1999
Racher KI; Voegele RT; Marshall DE; Culham DE; Wood JM; Jung H; Bacon M; Cairns MT; Ferguson SM; Liang WJ; Henderson PJF; White G; Hullett FR Purification and reconstitution of an osmosensor: Transporter ProP of Escherichia coli senses and responds to osmotic shifts ChemBioChem 38 1676-1684, 1999
Spooner PJR; Oreilly WJ; Homans SW; Rutherford N; Henderson PJF; Watts A Weak substrate binding to transport proteins studied by NMR Biophysical Journal 75 2794-2800, 1998
McDonald TP; Walmsley AR; Henderson PJF Asparagine 394 in putative helix 11 of the galactose-H+ symport protein (GalP) from Escherichia coli is associated with the internal binding site for cytochalasin B and sugar Journal of Biological Chemistry 272 15189-15199, 1997
Sanderson NM; Martin GEM; Rutherford NG; Henderson PJF Purification, reconstitution and circular dichroism of the galactose-H transport protein [GalP-(His)] of Escherichia coli Biochemical Society Transactions 25 -, 1997
Knol J; Veenhoff L; Liang WJ; Henderson PJF; Leblanc G; Poolman B Unidirectional reconstitution into detergent-destabilized liposomes of the purified lactose transport system of Streptococcus thermophilus Journal of Biological Chemistry 271 15358-15366, 1996
Poolman B; Knol J; vanderDoes C; Henderson PJF; Liang WJ; Leblanc G; Pourcher T; MusVeteau I Cation and sugar selectivity determinants in a novel family of transport proteins MOL MICROBIOL 19 911-922, 1996
McDonald TP; Walmsley AR; Martin GEM; Henderson PJF The role of tryptophans 371 and 395 in the binding of antibiotics and the transport of sugars by the D-galactose-H+ symport protein (GalP) from Escherichia coli J BIOL CHEM 270 30359-30370, 1995
SUMIYA M; DAVIS EO; PACKMAN LC; MCDONALD TP; HENDERSON PJF MOLECULAR-GENETICS OF A RECEPTOR PROTEIN FOR D-XYLOSE, ENCODED BY THE GENE XYLF IN ESCHERICHIA-COLI RECEPTOR CHANNEL 3 117-128, 1995
SHATWELL KP; CHARALAMBOUS BM; MCDONALD TP; HENDERSON PJF CLONING, SEQUENCING, AND EXPRESSION OF THE ARAE GENE OF KLEBSIELLA-OXYTOCA-8017, WHICH ENCODES ARABINOSE-H+ SYMPORT ACTIVITY J BACTERIOL 177 5379-5380, 1995
MARTIN GEM; RUTHERFORD NG; HENDERSON PJF; WALMSLEY AR KINETICS AND THERMODYNAMICS OF THE BINDING OF FORSKOLIN TO THE GALACTOSE-H+ TRANSPORT PROTEIN, GALP, OF ESCHERICHIA-COLI BIOCHEM J 308 261-268, 1995
GUNN FJ; TATE CG; SANSOM CE; HENDERSON PJF TOPOLOGICAL ANALYSES OF THE L-FUCOSE-H+ SYMPORT PROTEIN, FUCP, FROM ESCHERICHIA-COLI MOL MICROBIOL 15 771-783, 1995
MARTIN GEM; SEAMON KB; BROWN FM; SHANAHAN MF; ROBERTS PE; HENDERSON PJF FORSKOLIN SPECIFICALLY INHIBITS THE BACTERIAL GALACTOSE H+ TRANSPORT PROTEIN, GALP J BIOL CHEM 269 24870-24877, 1994
HENDERSON PJF; MARTIN GEM; MCDONALD TP; STEEL A; WALMSLEY AR DISSECTION OF DISCRETE KINETIC EVENTS IN THE BINDING OF ANTIBIOTICS AND SUBSTRATES TO THE GALACTOSE-H+ SYMPORT PROTEIN, GALP, OF ESCHERICHIA-COLI ANTON LEEUW INT J G 65 349-358, 1994
WALMSLEY AR; MARTIN GEM; HENDERSON PJF 8-ANILINO-1-NAPHTHALENESULFONATE IS A FLUORESCENT-PROBE OF CONFORMATIONAL-CHANGES IN THE D-GALACTOSE-H+ SYMPORT PROTEIN OF ESCHERICHIA-COLI J BIOL CHEM 269 17009-17019, 1994
GUNN FJ; TATE CG; HENDERSON PJF IDENTIFICATION OF A NOVEL SUGAR-H+ SYMPORT PROTEIN, FUCP, FOR TRANSPORT OF L-FUCOSE INTO ESCHERICHIA-COLI MOL MICROBIOL 12 799-809, 1994
SPOONER PJR; RUTHERFORD NG; WATTS A; HENDERSON PJF NMR OBSERVATION OF SUBSTRATE IN THE BINDING-SITE OF AN ACTIVE SUGAR-H+ SYMPORT PROTEIN IN NATIVE MEMBRANES P NATL ACAD SCI USA 91 3877-3881, 1994
WALMSLEY AR; LOWE AG; HENDERSON PJF THE KINETICS AND THERMODYNAMICS OF THE BINDING OF CYTOCHALASIN-B TO SUGAR TRANSPORTERS EUR J BIOCHEM 221 513-522, 1994
TATE CG; HENDERSON PJF MEMBRANE TOPOLOGY OF THE L-RHAMNOSE-H+ TRANSPORT PROTEIN (RHAT) FROM ENTEROBACTERIA J BIOL CHEM 268 26850-26857, 1993
KWONG FYP; WU JSR; SHI MM; FINCHAM HE; DAVIES A; HENDERSON PJF; BALDWIN SA; YOUNG JD ENZYMATIC CLEAVAGE AS A PROBE OF THE MOLECULAR-STRUCTURES OF MAMMALIAN EQUILIBRATIVE NUCLEOSIDE TRANSPORTERS J BIOL CHEM 268 22127-22134, 1993
WALMSLEY AR; PETRO KR; HENDERSON PJF EQUILIBRIUM AND TRANSIENT KINETIC-STUDIES OF THE BINDING OF CYTOCHALASIN-B TO THE L-ARABINOSE-H+ SYMPORT PROTEIN OF ESCHERICHIA-COLI - DETERMINATION OF THE SUGAR BINDING-SPECIFICITY OF THE L-ARABINOSE-H+ SYMPORTER EUR J BIOCHEM 215 43-54, 1993
MUIRY JAR; GUNN TC; MCDONALD TP; BRADLEY SA; TATE CG; HENDERSON PJF PROTON-LINKED L-RHAMNOSE TRANSPORT, AND ITS COMPARISON WITH L-FUCOSE TRANSPORT IN ENTEROBACTERIACEAE BIOCHEM J 290 833-842, 1993
Henderson PJF The 12-transmembrane helix transporters Current Opinion in Cell Biology 5 708-721, 1993
View abstract

From the hydropathic profiles of their amino acid sequences many transport proteins are conceived to comprise 12-transmembraneα-helices. In only a few examples, however, is there genetical and/or biochemical evidence to support the 12-helix structure or illuminate the molecular mechanism of the transport process. A number of these transport proteins occur in evolutionarily related families, and sometimes superfamilies, indicating divergent evolution of the 12-helix structure. Other individual members or families of transport proteins are sufficiently different in amino acid sequence for their evolution to have taken place by convergence from independent ancestral origins.
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KWONG FYP; FINCHAM HE; DAVIES A; BEAUMONT N; HENDERSON PJF; YOUNG JD; BALDWIN SA MAMMALIAN NITROBENZYLTHIOINOSINE-SENSITIVE NUCLEOSIDE TRANSPORT PROTEINS - IMMUNOLOGICAL EVIDENCE THAT TRANSPORTERS DIFFERING IN SIZE AND INHIBITOR SPECIFICITY SHARE SEQUENCE HOMOLOGY J BIOL CHEM 267 21954-21960, 1992
TATE CG; MUIRY JAR; HENDERSON PJF MAPPING, CLONING, EXPRESSION, AND SEQUENCING OF THE RHAT GENE, WHICH ENCODES A NOVEL L-RHAMNOSE-H+ TRANSPORT PROTEIN IN SALMONELLA-TYPHIMURIUM AND ESCHERICHIA-COLI J BIOL CHEM 267 6923-6932, 1992
Griffith JK; Baker ME; Rouch DA; Page MGP; Skurray RA; Paulsen IT; Chater KF; Baldwin SA; Henderson PJF Membrane transport proteins: Implications of sequence comparisons Current Opinion in Cell Biology 4 684-695, 1992
DOI:10.1016/0955-0674(92)90090-Y
Dent HC; Henderson PJF; Lucas VA Purification of the galactose/H symport protein of Escherichia coli Biochemical Society Transactions 20 -, 1992
CAIRNS MT; MCDONALD TP; HORNE P; HENDERSON PJF; BALDWIN SA CYTOCHALASIN-B AS A PROBE OF PROTEIN-STRUCTURE AND SUBSTRATE RECOGNITION BY THE GALACTOSE/H+ TRANSPORTER OF ESCHERICHIA-COLI J BIOL CHEM 266 8176-8183, 1991
Henderson PJF Sugar transport proteins Current Opinion in Structural Biology 1 590-601, 1991
View abstract

Proteins that catalyse the transport of sugars may be fundamentally similar to proteins that catalyse the transport of many other substrates, both into and out of cells. The number of sugar transport proteins with known amino acid sequences has increased considerably, but there is still insufficient understanding of their three-dimensional structures. Recent technological advances offer hope of being able to determine these three-dimensional structures.© 1991 Current Biology Ltd.
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HENDERSON PJF THE HOMOLOGOUS GLUCOSE-TRANSPORT PROTEINS OF PROKARYOTES AND EUKARYOTES RES MICROBIOL 141 316-327, 1990
HENDERSON PJF; MAIDEN MCJ HOMOLOGOUS SUGAR-TRANSPORT PROTEINS IN ESCHERICHIA-COLI AND THEIR RELATIVES IN BOTH PROKARYOTES AND EUKARYOTES PHILOS T ROY SOC B 326 391-410, 1990
CHARALAMBOUS BM; MAIDEN MCJ; MCDONALD TP; CUNNINGHAM IJ; HENDERSON PJF DETECTION OF PROTON-LINKED SUGAR-TRANSPORT PROTEINS IN ENTEROBACTERIACEAE BIOCHEM SOC T 17 441-441, 1989
KWONG FYP; DAVIES A; TSE CM; YOUNG JD; HENDERSON PJF; BALDWIN SA PURIFICATION OF THE HUMAN-ERYTHROCYTE NUCLEOSIDE TRANSPORTER BY IMMUNOAFFINITY CHROMATOGRAPHY BIOCHEM J 255 243-249, 1988
MAIDEN MCJ; JONESMORTIMER MC; HENDERSON PJF THE CLONING, DNA-SEQUENCE, AND OVEREXPRESSION OF THE GENE ARAE CODING FOR ARABINOSE-PROTON SYMPORT IN ESCHERICHIA-COLI-K12 J BIOL CHEM 263 8003-8010, 1988
BRADLEY SA; TINSLEY CR; MUIRY JAR; HENDERSON PJF PROTON-LINKED L-FUCOSE TRANSPORT IN ESCHERICHIA-COLI BIOCHEM J 248 495-500, 1987
DAVIS EO; HENDERSON PJF THE CLONING AND DNA-SEQUENCE OF THE GENE XYLE FOR XYLOSE-PROTON SYMPORT IN ESCHERICHIA-COLI-K12 J BIOL CHEM 262 13928-13932, 1987
HENDERSON PJF; MAIDEN MCJ SUGARS, ANTIBIOTICS, MICROBES AND MEN TRENDS GENET 3 62-64, 1987
MAIDEN MCJ; DAVIS EO; BALDWIN SA; MOORE DCM; HENDERSON PJF MAMMALIAN AND BACTERIAL SUGAR-TRANSPORT PROTEINS ARE HOMOLOGOUS NATURE 325 641-643, 1987
HENDERSON PJF; BRADLEY S; MACPHERSON AJS; HORNE P; DAVIS EO; DARUWALLA KR; JONESMORTIMER MC SUGAR PROTON TRANSPORT-SYSTEMS OF ESCHERICHIA-COLI BIOCHEM SOC T 12 146-148, 1984
MACASKIE LE; SHEARD AG; DAINTY RH; HENDERSON PJF GLYCEROL UTILIZATION BY BROCHOTHRIX-THERMOSPHACTA J APPL BACTERIOL 56 137-143, 1984
DAVIS EO; JONESMORTIMER MC; HENDERSON PJF LOCATION OF A STRUCTURAL GENE FOR XYLOSE-H+ SYMPORT AT 91 MIN ON THE LINKAGE MAP OF ESCHERICHIA-COLI-K12 J BIOL CHEM 259 1520-1525, 1984
FOULKES JG; STRADA SJ; HENDERSON PJF; COHEN P A KINETIC-ANALYSIS OF THE EFFECTS OF INHIBITOR-1 AND INHIBITOR-2 ON THE ACTIVITY OF PROTEIN PHOSPHATASE-1 EUR J BIOCHEM 132 309-313, 1983
MACPHERSON AJS; JONESMORTIMER MC; HORNE P; HENDERSON PJF IDENTIFICATION OF THE GALP GALACTOSE TRANSPORT PROTEIN OF ESCHERICHIA-COLI J BIOL CHEM 258 4390-4396, 1983
HORNE P; HENDERSON PJF THE ASSOCIATION OF PROTON MOVEMENT WITH GALACTOSE TRANSPORT INTO SUBCELLULAR MEMBRANE-VESICLES OF ESCHERICHIA-COLI BIOCHEM J 210 699-705, 1983
BULLOUGH DA; JACKSON CG; HENDERSON PJF; BEECHEY RB; LINNETT PE THE ISOLATION AND PURIFICATION OF THE ELVAPEPTINS - A FAMILY OF PEPTIDE INHIBITORS OF MITOCHONDRIAL ATPASE ACTIVITY FEBS LETT 145 258-262, 1982
BULLOUGH DA; JACKSON CG; HENDERSON PJF; COTTEE FH; BEECHEY RB; LINNETT PE THE AMINO-ACID-SEQUENCE OF EFRAPEPTIN-D BIOCHEM INT 4 543-549, 1982
DARUWALLA KR; PAXTON AT; HENDERSON PJF ENERGIZATION OF THE TRANSPORT-SYSTEMS FOR ARABINOSE AND COMPARISON WITH GALACTOSE TRANSPORT IN ESCHERICHIA-COLI BIOCHEM J 200 611-627, 1981
MACPHERSON AJS; JONESMORTIMER MC; HENDERSON PJF IDENTIFICATION OF THE ARAE TRANSPORT PROTEIN OF ESCHERICHIA-COLI BIOCHEM J 196 269-283, 1981
MACASKIE LE; DAINTY RH; HENDERSON PJF THE ROLE OF THIAMINE AS A FACTOR FOR THE GROWTH OF BROCHOTHRIX-THERMOSPHACTA J APPL BACTERIOL 50 267-273, 1981
LAM VMS; DARUWALLA KR; HENDERSON PJF; JONESMORTIMER MC PROTON-LINKED D-XYLOSE TRANSPORT IN ESCHERICHIA-COLI J BACTERIOL 143 396-402, 1980
Jackson CG; Linnett PE; Beechey RB; Henderson PJF Purification and Preliminary Structural Analysis of the Efrapeptins, a Group of Antibiotics that Inhibit the Mitochondrial Adenosine Triphosphatase BIOCHEMICAL SOCIETY TRANSACTIONS 7 224-226, 1979
DOI:10.1042/bst0070224
HENDERSON PJF; GIDDENS RA 2-DEOXY-D-GALACTOSE, A SUBSTRATE FOR GALACTOSE-TRANSPORT SYSTEM OF ESCHERICHIA-COLI BIOCHEM J 168 15-22, 1977
HENDERSON PJF; GIDDENS RA; JONESMORTIMER MC TRANSPORT OF GALACTOSE, GLUCOSE AND THEIR MOLECULAR ANALOGS BY ESCHERICHIA-COLI-K12 BIOCHEM J 162 309-320, 1977
Henderson PJF; Skinner A Association of proton movements with the galactose and arabinose transport systems of Escherichia coli Biochemical Society Transactions 2 543-545, 1974
HENDERSO.PJ STEADY-STATE ENZYME-KINETICS WITH HIGH-AFFINITY SUBSTRATES OR INHIBITORS - STATISTICAL TREATMENT OF DOSE-RESPONSE CURVES BIOCHEM J 135 101-107, 1973
Henderson PJF; Lardy HA; Dorschner E Factors affecting the inhibition of adenine nucleotide translocase by bongkrekic acid Biochemistry 9 3453-3457, 1970
View abstract

The translocation of adenine nucleotides and their structural analogs across the membranes of rat liver mitochondria is inhibited by bongkrekic acid; the extent of inhibition achieved depends on the ratio of bongkrekic acid concentration to mitochondrial protein concentration, the temperature, and period of time for which the mitochondria are exposed to the inhibitor. By contrast, inactivation of the translocase enzyme by atractyloside is relatively independent of these parameters. The greater potency of bongkrekic acid, when compared to atractyloside on a concentration basis, is probably due to the relatively weak antagonism by adenosine diphosphate, whereas adenine nucleotides competitively diminish inhibition by atractyloside.
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