Faculty of Biological Sciences

Prof Jurgen Denecke

MSc (Eng), Brussels; PhD 1991, Ghent.
Professor of Plant Cell Biology & Biotechnology
School of Biology

Contact:  Miall 9.15, +44(0) 113 34 33056/37031, email address for  

You can read more about Prof Denecke's interests here:
www.plants.leeds.ac.uk/people/groups_den.php
www.fbs.leeds.ac.uk/staff/Denecke/

Research Interests

Plant cell secretory pathway

Faculty Research and Innovation



Studentship information

Postgraduate studentship areas:

  • Plant Cell Biology; Protein folding and transport/pathway engineering/biofermentation and biotechnology

See also:

Modules managed

BIOL1112 - The Molecules of Life
BLGY1115 - Introduction to Cell Biology: from Molecules to Cells and Tissues
BLGY1125 - Biology Practicals and Data Analysis
FOBS1135 - The Basis of Life

Modules taught

BLGY1125 - Biology Practicals and Data Analysis
BLGY1211 - Applied Biology and Agriculture
BLGY1236 - Practical Applied Biology
BLGY1303 - Tutorials for Biology and Genetics
BLGY2100 - Enhanced Study Skills for Biologists
BLGY3021 - Research Project
BLGY3340 - Biology Research Projects
FOBS1135/BIOL1112/BLGY1115 - The Basis of Life/The Molecules of Life/Introduction to Cell Biology
FOBS1135/BLGY1115 - The Basis of Life/Introduction to Cell Biology: from Molecules to Cells and Tissues

Centre membership: The Centre for Plant Sciences

Group Leader Prof Jurgen Denecke  (Professor of Plant Cell Biology & Biotechnology)

Plant cell secretory pathway 

Postgraduates

Jonas Alvim (Primary supervisor) 50% FTE
Rajni Bhardwaj (Co-supervisor) 25% FTE

Gershlick DC, De Marcos Lousa CDEM, Foresti O, Lee AJ, Pereira EA, daSilva LL, Bottanelli F, Denecke J Golgi-dependent transport of vacuolar sorting receptors is regulated by COPII, AP1, and AP4 protein complexes in tobacco. The Plant Cell 26 1308-1329, 2014
DOI:10.1105/tpc.113.122226
View abstract

Lousa CDM, Gershlick DC, Denecke J Mechanisms and Concepts Paving the Way towards a Complete Transport Cycle of Plant Vacuolar Sorting Receptors PLANT CELL 24 1714-1732, 2012
DOI:10.1105/tpc.112.095679

Bottanelli F, Gershlick DC, Denecke J Evidence for sequential action of Rab5 and Rab7 GTPases in prevacuolar organelle partitioning. Traffic 13 338-354, 2012
DOI:10.1111/j.1600-0854.2011.01303.x
View abstract

Bottanelli F, Foresti O, Hanton S, Denecke J Vacuolar Transport in Tobacco Leaf Epidermis Cells Involves a Single Route for Soluble Cargo and Multiple Routes for Membrane Cargo PLANT CELL 23 3007-3025, 2011
DOI:10.1105/tpc.111.085480

Foresti O, Gershlick DC, Bottanelli F, Hummel E, Hawes C, Denecke J A Recycling-Defective Vacuolar Sorting Receptor Reveals an Intermediate Compartment Situated between Prevacuoles and Vacuoles in Tobacco PLANT CELL 22 3992-4008, 2010
DOI:10.1105/tpc.110.078436

Foresti O, Denecke J Intermediate organelles of the plant secretory pathway: Identity and function TRAFFIC 9 1599-1612, 2008
DOI:10.1111/j.1600-0854.2008.00791.x

Lousa CDM, Denecke J Lysosomal and vacuolar sorting: not so different after all! Biochemical Society Transactions 44 891-897, 2016
DOI:10.1042/BST20160050
View abstract

Gershlick DC, De Marcos Lousa C, Farmer L, Denecke J Routes to and from the plasma membrane: Bulk flow versus signal mediated endocytosis Plant Signaling and Behavior 9 -, 2014
DOI:10.4161/15592316.2014.972813
View abstract

Sutter J-U, Denecke J, Thiel G Synthesis of vesicle cargo determines amplitude of Ca(2+)-sensitive exocytosis. Cell Calcium 52 283-288, 2012
DOI:10.1016/j.ceca.2012.05.011
View abstract

Zhu Q, Dugardeyn J, Zhang C, Takenaka M, Kühn K, Craddock C, Smalle J, Karampelias M, Denecke J, Peters J, Gerats T, Brennicke A, Eastmond P, Meyer EH, Van Der Straeten D SLO2, a mitochondrial pentatricopeptide repeat protein affecting several RNA editing sites, is required for energy metabolism. Plant J 71 836-849, 2012
DOI:10.1111/j.1365-313X.2012.05036.x
View abstract

Denecke J, Aniento F, Frigerio L, Hawes C, Hwang I, Mathur J, Neuhaus J-M, Robinson DG Secretory pathway research: the more experimental systems the better. Plant Cell 24 1316-1326, 2012
DOI:10.1105/tpc.112.096362
View abstract

Bubeck J, Scheuring D, Hummel E, Langhans M, Viotti C, Foresti O, Denecke J, Banfield DK, Robinson DG The syntaxins SYP31 and SYP81 control ER-Golgi trafficking in the plant secretory pathway TRAFFIC 9 1629-1652, 2008
DOI:10.1111/j.1600-0854.2008.00803.x

Ribeiro D, Foresti O, Denecke J, Wellink J, Goldbach R, Kormelink RJM Tomato spotted wilt virus glycoproteins induce the formation of endoplasmic reticulum- and Golgi-derived pleomorphic membrane structures in plant cells J GEN VIROL 89 1811-1818, 2008
DOI:10.1099/vir.0.2008/001164-0

Rojo E, Denecke J What is moving in the secretory pathway of plants? PLANT PHYSIOL 147 1493-1503, 2008
DOI:10.1104/pp.108.124552

Snowden CJ, Leborgne-Castel N, Wootton LJ, Hadlington JL, Denecke J In vivo analysis of the lumenal binding protein (BiP) reveals multiple functions of its ATPase domain PLANT J 52 987-1000, 2007

daSilva LL, Snapp EL, Denecke J, Lippincott-Schwartz J, Hawes C, Brandizzi F Membrane dynamics in the early secretory pathway (vol 26, pg 199, 2004) CRIT REV PLANT SCI 26 285-285, 2007
DOI:10.1080/07352680701723692

Foresti O, daSilva LLP, Denecke J Overexpression of the Arabidopsis syntaxin PEP12/SYP21 inhibits transport from the prevacuolar compartment to the lytic vacuole in vivo PLANT CELL 18 2275-2293, 2006
DOI:10.1105/tpc.105.040279

Dasilva LLP, Foresti O, Denecke J Targeting of the plant vacuolar sorting receptor BP80 is dependent on multiple sorting signals in the cytosolic tail PLANT CELL 18 1477-1497, 2006
DOI:10.1105/tpc.105.040394

Pimpl P, Taylor JP, Snowden C, Hillmer S, Robinson DG, Denecke J Golgi-mediated vacuolar sorting of the endoplasmic reticulum chaperone BiP may play an active role in quality control within the secretory pathway PLANT CELL 18 198-211, 2006
DOI:10.1105/tpc.105.036665

daSilva LLP, de Molfetta-Machado JB, Panunto-Castelo A, Denecke J, Goldman GH, Roque-Barreira MC, Goldman MHS cDNA cloning and functional expression of KM+, the mannose-binding lectin from Artocarpus integrifolia seeds Biochimica et Biophysica Acta: General Subjects 1726 251-260, 2005
View abstract

daSilva LLP, Taylor JP, Hadlington JL, Hanton SL, Snowden CJ, Fox SJ, Foresti O, Brandizzi F, Denecke J Receptor salvage from the prevacuolar compartment is essential for efficient vacuolar protein targeting PLANT CELL 17 132-148, 2005
DOI:10.1105/tpc.104.026351

daSilva LLP, Snapp EL, Denecke J, Lippincott-Schwartz J, hawes C, Brandizzi F Endoplasmic Reticulum Export Sites and Golgi Bodies Behave as Single Mobile Secretory Units in Plant Cells Plant Cell 16 1753-1771, 2004
DOI:10.1105/tpc.022673
View abstract

Hadlington JL, Santoro A, Nuttall J, Denecke J, Ma JKC, Vitale A, Frigerio L The C-terminal extension of a hybrid immunoglobulin A/G heavy chain is responsible for its golgi-mediated sorting to the vacuole Molecular Biology of the Cell 14 2592-2602, 2003
DOI:10.1091/mbc.E02-11-0771

Pimpl P, Hanton SL, Taylor JP, Pinto-daSilva LL, Denecke J The GTPase ARF1p controls the sequence-specific vacuolar sorting route to the lytic vacuole PLANT CELL 15 1242-1256, 2003
DOI:10.1105/tpc.010140

Brandizzi F, Hanton SL, da Silva L, Boevink P, Evans D, Oparka K, Denecke J, hawes C ER quality control can lead to retrograde transport from the ER lumen to the cytosol and the nucleoplasm in plants The Plant Journal 34 269-281, 2003
DOI:10.1046/j.1365-313X.2003.01728.x

Roberts M, Denecke J, Collinge D Untitled - Foreword PLANT MOL BIOL 50 819-821, 2002

Pimpl P, Denecke J Protein-protein interactions in the secretory pathway, a growing demand for experimental approaches in vivo PLANT MOL BIOL 50 887-902, 2002

Roberts M, Denecke J, Collinge D Plant Molecular Biology: Foreword Plant Molecular Biology 50 819-821, 2002
DOI:10.1023/A:1021297800500

Tormakangas K, Hadlington JL, Pimpl P, Hillmer S, Brandizzi F, Teeri TH, Denecke J A vacuolar sorting domain may also influence the way in which proteins leave the endoplasmic reticulum PLANT CELL 13 2021-2032, 2001

Phillipson BA, Pimpl P, daSilva LL, Crofts AJ, Taylor JP, Movafeghi A, Robinson DG, Denecke J Secretory bulk flow of soluble proteins is efficient and COPII dependent. Plant Cell 13 2005-2020, 2001
View abstract

Pimpl P, Movafeghi A, Coughlan S, Denecke J, Hillmer S, Robinson DG In situ localization and in vitro induction of plant COPI-coated vesicles PLANT CELL 12 2219-2235, 2000

Vitale A, Denecke J The Endoplasmic Reticulum Gateway of the Secretory Pathway. Plant Cell 11 615-628, 1999

Crofts AJ, Leborgne-Castel N, Hillmer S, Robinson DG, Phillipson B, Denecke J Saturation of the endoplasmic reticulum retention machinery reveals anterograde bulk flow. Plant Cell 11 2233-2247, 1999

Jelitto-Van Dooren E, Vidal S, Denecke J Anticipating endoplasmic reticulum stress: a novel early response before pathogenesis-related gene induction. Plant Cell 11 1935-1943, 1999

Leborgne-Castel N, Jelitto-Van Dooren E, Crofts AJ, Denecke J Overexpression of BiP in tobacco alleviates Endoplasmic Reticulum stress. Plant Cell 11 459-470, 1999

Crofts AJ, Legborgne-Castel N, Pesca M, Vitale A, Denecke J BiP and calreticulin form an abundant complex that is independent of Endoplasmic reticulum stress. Plant Cell 10 813-823, 1998
View abstract

Denecke J, Robinson DG, Vitale A The plant secretory system, mechanisms, pathways and applications in biotechnology. Journal of Experimental Botany 49 1073-1079, 1998

Crofts AJ, Denecke J Calreticulin and calnexin in plants. Trends in Plant Science 3 396-399, 1998

Denecke J Cell wall degrading enzymes from Erwinia carotovora cooperate in the salicylic acid-independent induction of a plant defense response Unknown Journal - Requested journal to be added to ULPD 11 23-32, 1998

Denecke J Salicylic acid and the plant pathogen Erwinia carotovora induce defense genes via antagonistic pathways The Plant Journal 11 115-123, 1997

Denecke J Mechanisms controlling function, identity and integrity of the plant endoplasmic reticulum. In Membranes: Specialized functions in plants. , 1996

Denecke J ER resident proteins and their function in protein synthesis and transport Plant Physiology and Biochemistry 34 197-205, 1996