Faculty of Biological Sciences

Prof Alex Breeze

Chair in Biomolecular NMR
School of Molecular and Cellular Biology

Contact:  Miall 10.28, +44(0) 113 34 30087, email address for  

Faculty Research and Innovation



Studentship information

See also:

Modules managed

BIOL5164M - Biopharmaceutical Development Pathway
BIOL5372M - Advanced Biomolecular Technologies

Modules taught

BIOC1301 - Introductory Integrated Biochemistry: the Molecules and Processes of Life
BIOC1303 - Introductory Biochemistry: Problem Solving and Data Handling
BIOC2301 - Intermediate Integrated Biochemistry
BIOC2303 - Intermediate Biochemistry: Skills
BIOC3160 - Laboratory/Literature/Computing Research Project
BIOC3221/22 c - ATU - Probing the structure of life
BIOC3231/32/3900/BIOL3211/MICR3212 - ATU - Molecular Oncology
BIOC3303 - Advanced Biochemistry: Skills
BIOL2303 - Intermediate Skills for Biotechnology
BIOL3398 - Research Tools and Applications
BIOL3399 - Extended Research Project Preparation
BIOL5164M - Biopharmaceutical Development Pathway
BIOL5382M - Extended Research Project
BIOW5901X - Foundation module
BIOW5904X - Folding and Assembly
BIOW5907X - Biocatalysis

Committees

Member of Graduate School Committee (Faculty representative on Graduate Board Scholarships, Studentships and Prizes Group)

Dr Simon Skinner  (Research Fellow)

Postgraduates

Brendan Farrell (Primary supervisor) 34% FTE
Janne Darell (Co-supervisor) 33% FTE
Katarzyna Haza (Co-supervisor) 30% FTE
Caroline Seiler (Co-supervisor) 50% FTE
Hugh Smith (Co-supervisor) 50% FTE

Schnieders R, Richter C, Warhaut S, de Jesus V, Keyhani S, Duchardt-Ferner E, Keller H, Wöhnert J, Kuhn LT, Breeze AL, Bermel W, Schwalbe H, Fürtig B Evaluation of<sup>15</sup>N-detected H–N correlation experiments on increasingly large RNAs Journal of Biomolecular NMR 1-14, 2017
DOI:10.1007/s10858-017-0132-7
View abstract

Breeze A, Bauer U “Ligandability” of Drug Targets: Assessment of Chemical Tractability via Experimental and In Silico Approaches In Lead Generation Methods and Strategies , 2016
View abstract

Patani H, Bunney TD, Thiyagarajan N, Norman RA, Ogg D, Breed J, Ashford P, Potterton A, Edwards M, Williams SV, Thomson GS, Pang CSM, Knowles MA, Breeze AL, Orengo C, Phillips C, Katan M Landscape of activating cancer mutations in FGFR kinases and their differential responses to inhibitors in clinical use. Oncotarget 7 24252-24268, 2016
DOI:10.18632/oncotarget.8132
View abstract

Burslem GM, Kyle HF, Breeze AL, Edwards TA, Nelson A, Warriner SL, Wilson AJ Towards "bionic" proteins: Replacement of continuous sequences from HIF-1α with proteomimetics to create functional p300 binding HIF-1α mimics Chemical Communications 52 5421-5424, 2016
DOI:10.1039/c6cc01812b
View abstract

Burslem GM, Kyle HF, Prabhakaran P, Breeze AL, Edwards TA, Warriner SL, Nelson A, Wilson AJ Synthesis of highly functionalized oligobenzamide proteomimetic foldamers by late stage introduction of sensitive groups. Org Biomol Chem 14 3782-3786, 2016
DOI:10.1039/c6ob00078a
View abstract

Mellor R, Ciantar E, Breeze A A diagnostic dilemma: HELLP syndrome with concomitant primary immune thrombocytopenia in the third trimester of pregnancy, 2016

Breeze A, Ward RA, Winter J Fragment-based discovery of inhibitors of lactate dehydrogenase A In Fragment-based Drug Discovery Lessons and Outlook , 2016
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Vo U, Vajpai N, Flavell L, Bobby R, Breeze AL, Embrey KJ, Golovanov AP Monitoring ras interactions with the nucleotide exchange factor son of sevenless (Sos) using site-specific NMR reporter signals and intrinsic fluorescence Journal of Biological Chemistry 291 1703-1718, 2016
DOI:10.1074/jbc.M115.691238
View abstract

Klein T, Vajpai N, Phillips JJ, Davies G, Holdgate GA, Phillips C, Tucker JA, Norman RA, Scott AD, Higazi DR, Lowe D, Thompson GS, Breeze AL Structural and dynamic insights into the energetics of activation loop rearrangement in FGFR1 kinase Nature Communications 6 -, 2015
DOI:10.1038/ncomms8877
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Kyle HF, Wickson KF, Stott J, Burslem GM, Breeze AL, Tiede C, Tomlinson DC, Warriner SL, Nelson A, Wilson AJ, Edwards TA Exploration of the HIF-1α/p300 interface using peptide and Adhiron phage display technologies Molecular BioSystems 11 2738-2749, 2015
DOI:10.1039/c5mb00284b
View abstract

Ma J, Cao Q, McLeod SM, Ferguson K, Gao N, Breeze AL, Hu J Target-based whole-cell screening by¹H NMR spectroscopy. Angew Chem Int Ed Engl 54 4764-4767, 2015
DOI:10.1002/anie.201410701
View abstract

Winter JJ, Anderson M, Blades K, Brassington C, Breeze AL, Chresta C, Embrey K, Fairley G, Faulder P, Finlay MR, Kettle JG, Nowak T, Overman R, Patel SJ, Perkins P, Spadola L, Tart J, Tucker JA, Wrigley G Small molecule binding sites on the Ras:SOS complex can be exploited for inhibition of Ras activation. Journal of Medicinal Chemistry 58 2265-2274, 2015
DOI:10.1021/jm501660t
View abstract

Tucker JA, Klein T, Breed J, Breeze AL, Overman R, Phillips C, Norman RA Structural insights into FGFR kinase isoform selectivity: diverse binding modes of AZD4547 and ponatinib in complex with FGFR1 and FGFR4. Structure 22 1764-1774, 2014
DOI:10.1016/j.str.2014.09.019
View abstract

Burslem GM, Kyle HF, Breeze AL, Edwards TA, Nelson A, Warriner SL, Wilson AJ Small-molecule proteomimetic inhibitors of the HIF-1α-p300 protein-protein interaction ChemBioChem 15 1083-1087, 2014
DOI:10.1002/cbic.201400009
View abstract

Vajpai N, Schott A-K, Vogtherr M, Breeze AL NMR backbone assignments of the tyrosine kinase domain of human fibroblast growth factor receptor 1. Biomol NMR Assign 8 85-88, 2014
DOI:10.1007/s12104-013-9458-6
View abstract

Ma J, McLeod S, Maccormack K, Sriram S, Gao N, Breeze AL, Hu J Real-time monitoring of New Delhi metallo-β-lactamase activity in living bacterial cells by 1HNMR spectroscopy Angewandte Chemie - International Edition 53 2130-2133, 2014
DOI:10.1002/anie.201308636
View abstract

Klein T, Tucker J, Holdgate GA, Norman RA, Breeze AL FGFR1 kinase inhibitors: Close regioisomers adopt divergent binding modes and display distinct biophysical signatures ACS Medicinal Chemistry Letters 5 166-171, 2014
DOI:10.1021/ml4004205
View abstract

Breeze A Future Directions In Understanding and Exploiting Protein-Protein Interactions as drug Targets , 2013
DOI:10.4155/9781909453463

Vo U, Embrey KJ, Breeze AL, Golovanov AP 1H, 13C and 15N resonance assignment for the human K-Ras at physiological pH Biomolecular NMR Assignments 7 215-219, 2013
DOI:10.1007/s12104-012-9413-y
View abstract

Holdgate G, Geschwindner S, Breeze A, Davies G, Colclough N, Temesi D, Ward L Biophysical methods in drug discovery from small molecule to pharmaceutical Methods in Molecular Biology 1008 327-355, 2013
DOI:10.1007/978-1-62703-398-5_12
View abstract

Ward RA, Brassington C, Breeze AL, Caputo A, Critchlow S, Davies G, Goodwin L, Hassall G, Greenwood R, Holdgate GA, Mrosek M, Norman RA, Pearson S, Tart J, Tucker JA, Vogtherr M, Whittaker D, Wingfield J, Winter J, Hudson K Design and Synthesis of Novel Lactate Dehydrogenase A Inhibitors by Fragment-Based Lead Generation JOURNAL OF MEDICINAL CHEMISTRY 55 3285-3306, 2012
DOI:10.1021/jm201734r

Eakin AE, Green O, Hales N, Walkup GK, Bist S, Singh A, Mullen G, Bryant J, Embrey K, Gao N, Breeze A, Timms D, Andrews B, Uria-Nickelsen M, Demeritt J, Loch JT, Hull K, Blodgett A, Illingworth RN, Prince B, Boriack-Sjodin PA, Hauck S, MacPherson LJ, Ni H, Sherer B Pyrrolamide DNA gyrase inhibitors: Fragment-based nuclear magnetic resonance screening to identify antibacterial agents Antimicrobial Agents and Chemotherapy 56 1240-1246, 2012
DOI:10.1128/AAC.05485-11
View abstract

Edfeldt FNB, Folmer RHA, Breeze AL Fragment screening to predict druggability (ligandability) and lead discovery success Drug Discovery Today 16 284-287, 2011
DOI:10.1016/j.drudis.2011.02.002

Albert JS, Blomberg N, Breeze AL, Brown AJH, Burrows JN, Edwards PD, Folmer RHA, Geschwindner S, Griffen EJ, Kenny PW, Nowak T, Olsson LL, Sanganee H, Shapiro AB An integrated approach to fragment-based lead generation: Philosophy, strategy and case studies from AstraZeneca's drug discovery programmes Current Topics in Medicinal Chemistry 7 1600-1629, 2007
DOI:10.2174/156802607782341091
View abstract

Sullivan JE, Holdgate GA, Campbell D, Timms D, Gerhardt S, Breed J, Breeze AL, Bermingham A, Pauptit RA, Norman RA, Embrey KJ, Read J, VanScyoc WS, Ward WHJ Prevention of MKK6-dependent activation by binding to p38α MAP kinase Biochemistry 44 16475-16490, 2005
DOI:10.1021/bi051714v
View abstract

Black E, Breed J, Breeze AL, Embrey K, Garcia R, Gero TW, Godfrey L, Kenny PW, Morley AD, Minshull CA, Pannifer AD, Read J, Rees A, Russell DJ, Toader D, Tucker J Structure-based design of protein tyrosine phosphatase-1B inhibitors Bioorganic and Medicinal Chemistry Letters 15 2503-2507, 2005
DOI:10.1016/j.bmcl.2005.03.068
View abstract

Breeze A, Green OM, Hull KG, Ni H, Hauck SI, Mullen GB, Hales NJ, Timms D Pyrrol derivatives with antibacterial activity, 2005

Pauptit RA, Dennis CA, Derbyshire DJ, Breeze AL, Weston SA, Rowsell S, Murshudov GN NMR trial models: Experiences with the colicin immunity protein IM7 and the p85α C-terminal SH2-peptide complex Acta Crystallographica - Section D Biological Crystallography 57 1397-1404, 2001
DOI:10.1107/S0907444901012434
View abstract

Breeze AL Isotope-filtered NMR methods for the study of biomolecular structure and interactions Progress in Nuclear Magnetic Resonance Spectroscopy 36 323-372, 2000

Anderson M, Blowers D, Hewitt N, Hedge P, Breeze A, Hampton I, Taylor L Refolding, purification, and characterization of a loop deletion mutant of human Bcl-2 from bacterial inclusion bodies PROTEIN EXPRESSION AND PURIFICATION 15 162-170, 1999
DOI:10.1006/prep.1998.0996

Pauptit R, Weston S, Breeze A, Derbyshire D, Tucker A, Hales N, Hollinshead D, Timms D Antibacterial design based on the structures of gyrase-inhibitor complexes, 1998

Tsai FTF, Singh OMP, Skarzynski T, Wonacott AJ, Weston S, Tucker A, Pauptit RA, Breeze AL, Poyser JP, O'Brien R, Ladbury JE, Wigley DB The high-resolution crystal structure of a 24-kDa gyrase B fragment from E. coli complexed with one of the most potent coumarin inhibitors, clorobiocin Proteins: Structure, Function and Genetics 28 41-52, 1997
DOI:10.1002/(SICI)1097-0134(199705)28:1<41::AID-PROT4>3.0.CO;2-M
View abstract

Osborne MJ, Breeze AL, Lian LY, Reilly A, James R, Kleanthous C, Moore GR Three-dimensional solution structure and<sup>13</sup>C nuclear magnetic resonance assignments of the colicin E9 immunity protein Im9 Biochemistry 35 9505-9512, 1996
DOI:10.1021/bi960401k
View abstract

Breeze AL, Kara BV, Barratt DG, Anderson M, Smith JC, Luke RW, Best JR, Cartlidge SA Structure of a specific peptide complex of the carboxy-terminal SH2 domain from the p85α subunit of phosphatidylinositol 3-kinase EMBO Journal 15 3579-3589, 1996
View abstract

OSBORNE MJ, LIAN LY, BREEZE A, REILLY A, WALLIS R, JAMES R, KLEANTHOUS C, MOORE GR THE NMR-DERIVED SOLUTION STRUCTURE OF THE IMMUNITY PROTEIN OF COLICIN E9, 1995

WERNER JM, BREEZE AL, CAMPBELL ID SOLUTION NMR-STUDIES OF HUMAN GRANULOCYTE-COLONY-STIMULATING FACTOR, 1995

Werner JM, Boyd J, Soffe N, Campbell ID, Breeze AL, Kara B, Rosenbrock G Secondary Structure and Backbone Dynamics of Human Granulocyte Colony-Stimulating Factor in Solution Biochemistry 33 7184-7192, 1994
DOI:10.1021/bi00189a022
View abstract

BREEZE AL, CAMPBELL ID MULTINUCLEAR AND MULTIDIMENSIONAL NMR-STUDIES OF GRANULOCYTE COLONY-STIMULATING FACTOR, 1993

Booker GW, Breeze AL, Downing AK, Panayotou G, Gout I, Waterfield MD, Campbell ID Structure of an SH2 domain of the p85α subunit of phosphatidylinositol-3-OH kinase Nature 358 684-687, 1992
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Breeze AL, Harvey TS, Bazzo R, Campbell ID Solution structure of human calcitonin gene-related peptide by 1H NMR and distance geometry with restrained molecular dynamics. Biochemistry 30 575-582, 1991
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Black AR, Breeze AL, Othman IB, Dolly JO Involvement of neuronal acceptors for dendrotoxin in its convulsive action in rat brain Biochemical Journal 237 397-404, 1986

Dolly JO, Halliwell JV, Black JD, Williams RS, Pelchen-Matthews A, Breeze AL, Mehraban F, Othman IB Botulinum neurotoxin and dendrotoxin as probes for studies on transmitter release Journal de Physiologie 79 280-303, 1984
View abstract

Breeze A Fragment-Based Drug Design by NMR In ENCYCLOPEDIA OF SPECTROSCOPY AND SPECTROMETRY, 3RD ED ,