Faculty of Biological Sciences

Dynein c from Chlamydomonas flagella inner arms

Please acknowledge the following publication in any use of the images in these pages:
S.A. Burgess, M.L. Walker, H. Sakakibara, P.J. Knight & K. Oiwa (2003) Dynein structure and power stroke. Nature, 421, 715-718.

Stem flexibility

In the most common orientation of the particle on the carbon film (so-called left view) the downward-pointing stem emerges from the left side of the head. Classification of the stem shows it is flexible, especially at the narrow neck close to the head. Note that the stem of apo-molecules lies closer to the head than in ADP.Vi molecules, and emerges from the head much closer to the stalk. The base of the stem, which anchors dynein on the microtubule, appears similar in both states.
In both movies the stalk flickers randomly across its range of positions: this indicates that stem and stalk flex independently.

The numbers in the top left of the panels are the arbitrary class numbers of the individual averaged images; those in the lower right are the number of raw images used to create each average.

ADP.Vi Apo
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