The study, by a team from the University of Leeds’ Astbury Centre for Structural Molecular Biology and the John Innes Centre in Norwich, describes the structure of an empty version of Cowpea Mosaic Virus (CPMV) and the molecular “glue” that allows the virus to build itself and encapsulate its genome.
The findings, published in the journal Nature Communications and based on revolutionary new electron microscopy, may be a crucial step to eventually allowing scientists to build custom versions of the virus that can carry medicines into the body and target disease.
Lead author Dr Neil Ranson, Associate Professor of Structural Molecular Biology at the University of Leeds, said: “To use Cowpea Mosaic Virus as a drug delivery vehicle, we need to understand how it puts itself together, and to do that we need to understand its structure in solution in very fine detail.
“Just a couple of years ago, that was impossible because we simply couldn’t see complex biological systems in the detail required. A new generation of electron microscopes, however, is revolutionising our ability to peer into the virus’ inner workings and understand how we might make it work for us.”
The Nature Communications paper investigates vital steps to understanding how safe, plant-based virus-like particles could be created in the future.
Dr Ranson said: “The aim of our project is to understand how the virus can put itself together from very simple building blocks. If we understand that properly, we may be able to efficiently make the virus package drugs, and then target them towards specific places or diseases in the human body, such as cancer cells.
“Plant viruses are ideal for such work – they are a huge evolutionary distance from us. You can’t catch plant viruses. Our paper shows the structure of the empty virus shell in unprecedented detail, including a part of the protein that is essential for assembly but has never been seen before. The virus-like particles, which were made by our collaborators at the John Innes Centre in Norwich, have no genome and therefore no ability to reproduce themselves or mutate,” Dr Ranson said.
“We are left with elegant, highly efficient and stable structures that have evolved to a level of perfection that it is currently impossible for man-made designs to rival, and these could be a major asset in developing targeted medicines. We could in the future change the sequences on their protein shells and retarget them at the diseases we want to hit.”
The paper is a product of a revolution in electron microscopy—dubbed the “resolution revolution”—that is transforming the level of detail at which structural biologists can work. It includes some of the most detailed electron microscope structures of protein complexes yet published, and these form the basis of a detailed analysis of how the CPMV virus builds itself.
The researchers show how the virus constructs a highly symmetrical, protein shell from five-sided “pentons” each built from five copies of a protein subunit. At the heart of the assembly process is a segment of a key protein – the C-terminus of the small coat protein subunit – that acts as a dab of molecular glue to hold the pentons together as the virus’ outer structure is built.
The C-terminus is also essential for the virus to package its genes, but it is cleaved from the virus when it has done its job. This has made it impossible to observe using other structural techniques such as x-ray crystallography.
Dr Emma Hesketh, a University of Leeds Research Fellow and the first author of the paper in Nature Communications, said: “The basic unit is very simple, so the virus only needs a very small amount of information to make a large protein shell. Not only is it very efficient but CPMV is known for building a very stable structure that doesn’t break down easily. We need that stability if these structures are going to survive drug manufacture and be introduced into the human body.”
“The new electron microscopes used in this study allowed us to see the segment in detail and understand its real role,” Dr Hesketh said.
The team used new-generation 300-kilovolt electron microscopes equipped with direct electron-detecting cameras at the Medical Research Council (MRC) Laboratory of Molecular Biology in Cambridge. The microscopes are capable of more than 130,000-times magnification. Two of the latest generation of this type of electron microscope are part of a £17 million investment in the new Astbury Biostructure Laboratory and are due to be installed at the University of Leeds next year.
“This equipment is completely transforming the level of detail at which we can interact with molecules. The new microscopes have more power but are also more stable and have sensors that directly detect the electron beam, rather than indirectly detecting it with optical sensors as the previous generation did.
“In practice, that means that, for the first time, we are looking in atomic detail at the individual amino acids in complex biological systems. This opens the way to manipulating those amino acids and intervening in the function of molecules with unprecedented precision.”
The research was funded by the Biotechnology and Biological Sciences Research Council (BBSRC) as part of the project entitled “Untangling the processes of replication and encapsidation in Picornavirales” led by Prof. George Lomonossoff at the John Innes Centre.
Ralf Richter, David Brockwell, Eric Hewitt, Jessica Kwok, Emanuele Paci and MAPS/FMH, BBSRC (Jun 2017), £600,000
Eric Blair, Adrian Whitehouse, Nicola Stonehouse, Alison Baker, Richard Bayliss, Joan Boyes, Ryan Seipke, Sally Boxall and MAPS/FMH, BBSRC (Jun 2017), £376,000
Stefan Kepinski, Yoselin Benitez-Alfonso, Tom Bennett, Michelle Peckham, BBSRC (Jun 2017), £331,000
Roman Tuma, Lars Jeuken, Paul Millner, Sheena Radford, Peter Stockley and MAPS/FMH, BBSRC (Jun 2017), £222,000
Vas Ponnambalam, Darren Tomlinson, Stephen Wheatcroft, BHF (May 2017), £107,878
Graham Askew in collaboration with Bangor University, BBSRC (Mar 2017), £477,383
Stephen Muench, BBSRC (Mar 2017), £132,945
Nic Stonehouse, MRC (Mar 2017), £906,341
Bill Kunin, Steve Sait, BBSRC (Mar 2017), £602,831
Adrian Goldman, EU (Mar 2017), £546,576
Sheena Radford, Wellcome Trust (Mar 2017), £1,836,482
Tom Bennett, Royal Society (Mar 2017), £15,000
Jamie Johnston, Royal Society (Mar 2017), £15,000
Beatrice Filippi, Royal Society (Mar 2017), £15,000
Ryan Seipke, BBSRC (Feb 2017), £52,116
Mary O'Connell, BBSRC (Feb 2017), £46,986
Hannah Dugdale, NERC (Feb 2017), £504,138
Anastasia Zhuravleva, EPSRC (Jan 2017), £100,792
Richard Bayliss, Cancer Research UK (Jan 2017), £1,600,000
John Barr, EU (Jan 2017), £339,000
Mark Harris, Royal Society (Jan 2017), £250,000
Alison Dunn, NERC (Jan 2017), £105,000
Alex Breeze, Pancreatic Cancer Research Fund (Jan 2017), £180,000
Alison Dunn, NERC (Dec 2016), £18,000
Lisa Collins, BBSRC (Dec 2016), £1,681,835
Brendan Davies, Leverhulme Trust (Dec 2016), £247,555
Alan Benson, Mark Drinkhill, Ed White, British Heart Foundaion (Dec 2016), £217,223
Adrian Goldman, Royal Society (Dec 2016), £82,999
Lisa Roberts, Alex Breeze, Brendan Davies, Timothy Devinney, Oliver Harlen, Joseph Holden, Anthea Hucklesby, Pamela Jones, Philip Mellor, RCUK (Nov 2016), £484,172
Lisa Roberts, Alex Breeze, Brendan Davies, Timothy Devinney, Oliver Harlen, Joseph Holden, Anthea Hucklesby, Pamela Jones, Philip Mellor, Wellcome Trust (Nov 2016), £119,343
Katie Field, Rank Prize Funds (Nov 2016), £20,000
Jessica Kwok, Royal Society (Nov 2016), £14,948
John Ladbury, Cancer Research UK (Oct 2016), £4,250
Miriam Wittmann, Martin Stacey, Edward Vital, Lupus UK
(Oct 2016), £34,010
Valerie Speirs, NC3Rs
(Oct 2016), £90,000
Nicola Stonehouse, Morgan Herod, David Rowlands, BBSRC
(Sep 2016), £436,424
Joseph Cockburn, Wellcome Trust
(Sep 2016), £100,000
John Barr, Public Health England
(Sep 2016), £94,471
Helen Miller, DSM Nutritional Products A/S
(Sep 2016), £54,680
Steven Clapcote, Vitaflo International Ltd
(Sep 2016), £39,285
Juan Fontana Jordan De Urries
, Royal Society
(Sep 2016), £21,793
Jing Li, Sarah Calaghan, Mark Drinkhill, British Heart Foundation
(Sep 2016), £117,585
Sheena Radford, Alison Ashcroft, BBSRC (Sep 2016), £457,216
Patricija Van Oosten-Hawle, An-Jung Chen, David Westhead, NC3Rs
(Sep 2016), £354,456
Glyn Hemsworth, BBSRC (Sep 2016), £1,024,034
David Jayne, Paul Millner, MRC (Aug 2016), £207,860
Sheena Radford, Alison Ashcroft, BBSRC (Aug 2016), £457,215
Patricija Van Oosten-Hawle, Dave Westhead, An-Jung Chen, NC3Rs (Aug 2016), £354,456
Peter Henderson, EU - European Union
EU - European Union
(Jul 2016), £123,897
Adrian Goldman, EU - European Union
(Jul 2016), £116,290
Urwin, Howard Atkinson, NERC
(Jul 2016), £105,053
Eileen Ingham and colleagues in Engineering and M&H, EPSRC (Jul 2016), £3,867,449
Michael Colman, MRC (Jul 2016), £200,956
Tim Benton, Fresca Group Ltd
(Jul 2016), £52,082