Faculty of Biological Sciences

Research Bulletin

Virus 'barcodes' offer rapid detection of mutated strains

14th May 2012

Researchers are developing a way to 'barcode' viral diseases to test new outbreaks for potentially lethal mutations.

The Faculty's Dr Julian Hiscox and Dr John Barr are working with the Health Protection Agency Porton (HPA) to build a bank of molecular signatures that will help identify the severity of virus infection from characteristic changes seen in cells. Currently the team is barcoding different strains of influenza virus and human respiratory syncytial virus (HRSV) - a virus associated with the onset of asthma in young children.

"Diseases such as flu infect and hijack our cells, turning them into virus producing factories," says Dr Hiscox. "The infection causes the balance of proteins in a cell to change - some proteins are overproduced and others suppressed. Which proteins are affected and by how much varies depending on the type of virus, allowing us to identify a unique barcode of disease for each."

The research, published today (14 May) in Proteomics, investigates changes in lung cells infected with swine flu from the 2009 outbreak compared with seasonal flu. The team used a labelling technique called SILAC to measure and compare thousands of different proteins in a sample.

This technique was used alongside mass spectrometry to identify the proteins most affected by viral infection and used these as molecular signatures to provide the 'barcode' of disease. The paper reports how several processes in the cell were affected by the virus, with most changes seen in proteins involved in cell replication.

"Swine flu affects the lungs in a similar way to seasonal flu and this was reflected in the barcodes we found for each," explains Dr Barr. "Using this test might have been a way to identify how lethal the 2009 swine flu pandemic was going to be, lessening worldwide panic.

"Our next step is to test more lethal strains of flu, such as bird flu, to see how the barcodes differ. Flu virus frequently mutates, resulting in new strains which may be life-threatening and become pandemic. If we can test new strains using our method, we can determine their potential impact on health by comparing their barcode of disease to those of viruses already studied."

The group from Leeds has already barcoded two types of HRSV which can cause severe respiratory disease in young children. Co-author Professor Miles Carroll of HPA Porton says: "We have focused our work on common respiratory viruses, such as flu and HRSV, but this method could be applied to a wide variety of viruses, including tropical diseases that are prone to sudden outbreaks and can be lethal."

The research was funded by the National Institute for Health Research (NIHR), the Biotechnology and Biological Sciences Research Council (BBSRC) and the Medical Research Council (MRC).


Recent Grants

Alan Berry, Wellcome Trust (Oct 2015), £752,365

Urwin, Howard Atkinson, BBSRC (Oct 2015), £200,293

Eric Hewitt, Andrew Macdonald, Yorkshire Kidney Research Fund (Oct 2015), £46,621

Ade Whitehouse, Alison Ashcroft, Ian Carr, BBSRC (Sep 2015), £438,975

Dave Westhead, Leukaemia & Lymphoma Research Leukaemia & Lymphoma Research (Sep 2015), £430,567

Shaunna Burke, Andrea Utley, Sarah Astill, Arts Council of England (Sep 2015), £80,594

Samit Chakrabarty, Ronaldo Ichiyama, Intl Foundn for Research in Paraplegia (Aug 2015), £93,000

Anastasia Zhuravleva, BBSRC (Jul 2015), £483,019

Alex O'Neill, MRC (Jul 2015), £249,822

Ade Whitehouse, Richard Foster, Cancer Research UK (Jul 2015), £201,034

Ronaldo Ichiyama, Jim Deuchars, Sue Deuchars, Wings For Life Spinal Cord Research (Jul 2015), £123,895

Martin Stacey and colleagues in FMH, MRC (Jun 2015), £426,475

Adrian Goldman, Sarah Harris, Roman Tuma, BBSRC (Jun 2015), £420,693

Elwyn Isaac, EU (Jun 2015), £238,915

Christine Foyer, BBSRC (Jun 2015), £160,401

Adrian Goldman, EU (Jun 2015), £116,331

David Brockwell, Sheena Radford, Innovate UK (Jun 2015), £113,378

Yoselin Benitez-Alfonso, EPSRC (Jun 2015), £93,672

Michelle Peckham, Peter Knight, Thomas Edwards, BBSRC (May 2015), £404,987

Michelle Peckham, Ed White, Peter Knight, BHF (May 2015), £208,184

Dave Westhead, Sheena Radford, Alex Breeze, BBSRC (May 2015), £51,021

Steve Clapcote, Vitaflo International Ltd (May 2015), £33,703

Les Firbank, Joe Holden, Pippa Chapman, NERC (Apr 2015), £388,726

Samit Chakrabarty, David Steenson, BBSRC (Apr 2015), £120,103

Paul Millner, Gin Jose, Sarah Aickin, DSTL Porton Down (Apr 2015), £63,407

Chris Hassell, David Lewis, The Physiological Society (Apr 2015), £6,900

Andrew Tuplin, Royal Society (Mar 2015), £15,000

Yoselin Benitez-Alfonso, Royal Society (Mar 2015), £14,770

Patricija Van Oosten-Hawle, Royal Society (Mar 2015), £13,960

Stuart Egginton, BHF (Mar 2015), £272,979

Keith Hamer, Department of Energy & Climate Change (Mar 2015), £58,066

Andrew Macdonald, Yorkshire Kidney Research Fund (Mar 2015), £41,171

Les Firbank, DEFRA Dept for Env. Food & Rural Affairs (Feb 2015), £20,000

Ian Hope, Marie-Anne Shaw, BBSRC (Jan 2015), £381,998

Paul Knox, BBSRC (Jan 2015), £5,000

Andrew Peel, BBSRC (Jan 2015), £359,077

Christine Foyer, BBSRC (Jan 2015), £408,334

Dave Westhead and colleagues in Experimental Haematology, Cancer Research UK (Jan 2015), £700,521

Mike McPherson, Christoph Walti, DSTL Porton Down (Jan 2015), £625,125

Sheena Radford, Mark Harris, Peter Stockley, Alan Berry, Alex O'Neill, Thomas Edwards, Adrian Goldman, Anastasia Zhuravleva, Wellcome Trust (Jan 2015), £443,015

Alison Ashcroft, Peter Stockley, Sheena Radford, Nicola Stonehouse, David Brockwell, Darren Tomlinson, BBSRC (Jan 2015), £340,937

Bill Kunin, EU (Jan 2015), £157,490

John Colyer, Leeds Teaching Hospitals Charitable Fund (Jan 2015), £40,000

Chris Hassall, Royal Society (Dec 2014), £14,500

Ryan Seipke, Royal Society (Nov 2014), £13,700

Neil Ranson, BBSRC (Nov 2014), £355,253

Alan Berry, Wellcome Trust (Oct 2014), £749,865

Les Firbank, Joe Holden, BBSRC (Oct 2014), £210,302

Darren Tomlinson and colleagues in Chemistry and Pathology, anatomy and Tumour Biology, Dr Hadwen Trusy (Oct 2014), £194,475

Paul Knox, EU (Oct 2014), £167,229

Martin Stacey and colleagues in Medicine & Health, Pfizer (Oct 2014), £90,453

Darren Tomlinson and colleagues in Experimental Oncology, YCR (Oct 2014), £69,480

Andrew Macdonald, Jamel Mankouri, Kidney Research Fund UK (Oct 2014), £58,878

Mike McPherson and colleagues in Dentistry and Engineering, Wellcome Trust (Oct 2014), £58,437

Dave Westhead and colleagues in Experimental Haemotology, Leukaemia & Lymphoma Research (Sep 2014), £281,424

Emmanuel Paci and colleagues in Chemistry, BBSRC (Sep 2014), £636,759

Andrew Peel, BBSRC (Sep 2014), £371,598

Lars Jeuken, Stephen Evans, BBSRC (Sep 2014), £333,684

Michelle Peckham, Mark Harris, Rao Sivaprasadarao, Eileen Ingham, Nic Stonehouse, Nikita Gamper, Wellcome Trust (Sep 2014), £192,763

Neil Ranson, BBSRC (Aug 2014), £355,253

Stuart Egginton, BHF (Aug 2014), £271,094

Darren Tomlinson, Mike McPherson, Technology Strategy Board (Aug 2014), £98,665

Peter Henderson, Leverhulme Trust (Aug 2014), £15,222

Recent
News

Leeds academics recognised for teaching excellence

11th June 2015

Two University of Leeds academics have received a prestigious teaching award, a National Teaching Fellowship.


The Quest to Engineer the Perfect Potato

8th June 2015

Researchers in the U.K. aim for a new commercial potato that resists many of the worst vulnerabilities of potato crops around the world.


University of Leeds announces £17m Astbury BioStructure Laboratory

3rd June 2015

The new facility will provide the University's internationally renowned Astbury Centre for Structural Molecular Biology with instruments for Electron Microscopy and Nuclear Magnetic Resonance that are competitive with the very best in the world.


The Cutlers Surgical Prize

18th May 2015

Dr Dan Saleh and Dr Andrea Utley win one of the most prestigious surgical prizes with their jointly developed Yorkshire Micro Forcep.


Leeds academics recruited to find pigs breakthrough

12th May 2015

A FIELD trial which is intended to lead to improved productivity in the British pig industry is being led by a group of academics in West Yorkshire.


Fascination of Plants Day 2015

7th May 2015

Plants are essential for life, providing everything, from food to energy, clothing and life-saving drugs.

 


Prestigious Royal Society of Chemistry award for Leeds research

7th May 2015

A research team led by Professors Alison Ashcroft and Sheena Radford joins an illustrious list of winners of the Royal Society of Chemistry's Rita and John Cornforth Award.


New project to develop 'smart' pesticides

27th April 2015

University of Leeds researchers are playing a key role in an international collaboration to develop new, eco-friendly pesticides.


A bit of hot water can prevent the spread of invasive species

20th April 2015

When it comes to invasive species in the United Kingdom, a few ounces of hot water may be worth nearly £2 billion in annual management costs, according to a new study.


Impact
Stories

Professor Eileen Ingham has led research over many years to develop improved approaches to aid healing and repair of soft tissues.

Dr Utley has carried out research over a number of years to increase understanding of the issues faced by children with varying types of movement and co-ordination difficulties.

Research carried out by Dr Ichiyama has led to the development of a rehabilitation training regime which allows an animal with spinal cord injury causing complete paralysis to walk again.

Professor Paul Milner has led a team of scientists on a project to develop antibody-based biosensor technologies.

All impact stories