Jeuken Lab Protocols and Methods

This page is still under construction and not all protocols and methods are available via the web yet

See links on the left hand side for an alphabetical order of our lab methods. The same links are given below, but catagorised in subject areas.

Molecular Biology

• Competent Cells

Protein Analysis

• BCA Assay
• SDS PAGE gels
• SDS PAGE electrophoresis
• Coommassie Staining
• Heme staining
• Ponceau Staining
• Western Blotting, including semi-dry protein transfer and imaging

Expression, purification and lipid reconstitution of proteins

• Cell disruption
• Cytochrome bo₃ reconstitution
• Expression and purification of CymA from Shewanella oneidensis MR-1
• Expression and purification of Cytochrome bo₃ from C43
• Expression and purification of Cytochrome bo₃ from GO105
• Expression and purification of his-tagged NupC
• Expression and purification of strep-tagged NupC
• Expression and purification of TEV
• Expression of untagged NupC
• Sucrose Gradient, Separation Inner and Outer membrane

Contact

Lars J. C. Jeuken
School of Biomedical Sciences
Leeds LS2 9JT UK
+44 (0)113 - 3433829
L.J.C.Jeuken@leeds.ac.uk
Office: Garstang (Bioincubator), 7.31

• Protocols

Tools

• PDB Databank
• Web of Knowledge
• Scopus

Networks

• CBMNet: Crossing Biological Membranes
• Metals in Biology
• Beyond the Molecule

@Leeds

• Astbury

Societies

• International Society of Electrochemistry
• Royal Society of Chemistry
• Inorganic Biochemistry Discussion Group
• British Biophysical Society
• Biochemical Society