Faculty of Biological Sciences

Equipment

The NMR facility of the Astbury BioStructure Laboratory (ABSL) houses 3 modern NMR spectrometers featuring proton (1H) resonance frequencies of  950, 750, and 600 MHz, respectively. These spectrometers are dedicated to the analysis of the structure and dynamics of biomolecules - mainly proteins, but also DNA, RNA and other biologically relevant compounds - in solution. All of the spectrometers are equipped with state-of-the-art cryoprobes exhibting unique capabilities of complementary nature. More detailed information for each of the magnets and their associated probes is listed below. In addition, our NMR facility features a small wet lab facility which is well suited for the preparation of NMR samples. IT infrastructure housed in the office adjacent to the magnet hall is available for the recording, processing, and the analysis of NMR data.

950 MHz Bruker Ascend Aeon™ NMR magnet

  • Next-generation ultra-high field NMR magnet; actively shielded and with helium recycling technology
  • TXO-cryoprobe (5mm); allows the use of 15N and 13C direct-detect NMR methods
  • TCI-cryoprobe (3mm); 1H-optimized triple resonance NMR 'inverse' probe; allows the use of small sample volumes; tolerant towards high-ionicity samples
  • Bruker Avance III HD console

750 MHz Oxford NMR magnet

  • TCI-cryoprobe; 1H-optimized triple resonance NMR 'inverse' probe
  • Bruker Avance III HD console

600 MHz Oxford NMR magnet

  • QCI-P-cryoprobe; proton-optimized quadruple resonance NMR 'inverse' probe
  • enables sensitive direct detection of phosphorus (31P)
  • ideally suited to the study of RNA or DNA, whilst also enabling research on proteins (e.g. phosphorylation), small molecules and metabolomic samples.
  • Bruker Avance III HD