Equipment

 

Becton Dickinson BD-LSRFortessa (Miall 5.13)

****** This Machine is being replaced with a Cytoflex S (4 Laser) system which has recently been installed. More details to follow shortly but please contact the facility for more information ******

 

Purchased in 2011 the Fortessa will detect up to 11 colours and full training on its operation is provided. Emission filters and dichroics are interchangeable if required.

Ideally suited to performing multicolour analysis for cell cycle assays, cell proliferation, apoptosis and multicolour immunophenotyping.

Software: DiVa 6

Specifications:  

488nm Blue Laser

  • 530/30 FITC / GFP / Alexa Fluor 488 / YFP
  • 575/26 PE / DsRed (not optimal excitation with 488 laser)
  • 610/20 PI / PE-Texas Red / 7-AAD / mCherry (not optimal excitation with 488 laser)
  • 695/40 PerCP-Cy5.5
  • 780/60 PE-Cy7

640nm Red Laser

  • 670/14 APC / TOPRO-3 / Cy5 / A633 / A647
  • 730/45 Alexa Fluor 700
  • 780/60 APC-Cy7

405 violet laser

  • 450/40 Pacific Blue / DAPI / Heochst
  • 525/50 AmCyan
  • 605/12 QDot 605

Additional filters available: 585/42 (DsRed) and 675/20 (PE-Cy5, PerCP)

 

 

Becton Dickinson FACSMelody

New Cell sorter installed February 2018.

More details to follow shortly, but please contact the facility for further information.