Faculty of Biological Sciences

Equipment

Becton Dickinson BD-LSRFortessa (Miall 5.13)

Purchased in 2011 this analyzer will detect up to 11 colours and full training on its operation is provided. Emission filters and dichroics are interchangeable if required.

Ideally suited to performing multicolour analysis for cell cycle assays, cell proliferation, apoptosis and multicolour immunophenotyping.

Software: DiVa 6

Specifications:  

488nm Blue Laser

  • 530/30 FITC / GFP / Alexa Fluor 488 / YFP
  • 575/26 PE / DsRed (not optimal excitation with 488 laser)
  • 610/20 PI / PE-Texas Red / 7-AAD / mCherry (not optimal excitation with 488 laser)
  • 695/40 PerCP-Cy5.5
  • 780/60 PE-Cy7

640nm Red Laser

  • 670/14 APC / TOPRO-3 / Cy5 / A633 / A647
  • 730/45 Alexa Fluor 700
  • 780/60 APC-Cy7

405 violet laser

  • 450/40 Pacific Blue / DAPI / Heochst
  • 525/50 AmCyan
  • 605/12 QDot 605

Additional filters available: 585/42 (DsRed) and 675/20 (PE-Cy5, PerCP)

Becton Dickinson FACSAria II (Miall 5.13)

Leeds Flow Cytometry Facility

9 colour analysis and particle sorting. Up graded with improved fluidics and additional violet laser in 2011. Emission filters and dichroics are interchangeable if required.

This machine is a high-speed sorter, and is principally used by the operator only; labs doing lots of sorting can have a user trained on the Aria.  To book a sort on the FACSAria, please contact the Facility Manager by email or by phoning 37270.

Software: DiVa 6

Specifications:  

488nm Blue Laser

  • 525/10 FITC / EGFP / A488 / EYFP
  • 585/21 PE
  • 610/10 PI / PE-Cy5
  • 695/20 PE-Texas Red
  • 780/30 PE-Cy7

633nm Red Laser

  • 660/10 APC / TOPRO-3 / Cy5 / A633 / A647
  • 780/30 APC-Cy7

405 violet laser

  • 450/40 DAPI / Heochst / ECFP / Pacific Blue
  • 530/30 Pacific Orange

Additional filters available: 585/42 and 675/20 (PE-Cy5, PerCP)