Delta Vision Widefield Deconvolution microscope

Leeds bioimagingLeeds Bio-imaging facility

Capabilities

  • Epi-fluorescent imaging of fixed and live cells
  • Improved image resolution via optimised deconvolution processing
  • High degree of sensitivity
  • Ideal for imaging light sensitive samples
  • Temperature, humidity and CO2 control for long time lapse imaging of live cells
  • Precision XYZ stage for multi point imaging and tiling
  • Differential Interference Contrast (DIC) imaging

Location

  • L.C.Miall 5.08

Cost

  • See Charges page (internal link only)

 

Filter Sets:

  • DAPI (ex 360/40) em 457/50
  • FITC / GFP / Cy2 (ex 490/20) em 528/38
  • TRITC / Rhodamine / Cy3 / DsRed (ex 555/28) em 617/73
  • Cy5 / A647 (ex 640/20) em 685/40
  • CFP (ex 430/10) em 470/30
  • YFP (ex 501/20) em 535/30


Objectives and hardware:

  • 10x/0.40, U Plan Apo
  • 20x/0.75, U PlanS Apo
  • 40x/1.35 Oil Iris, U Apo/340 (oil)
  • 60x/1.4 Oil, Plan Apo (oil)
  • 100x/1.4 Oil Iris, U Plan Apo (oil)
  • Motorised XYZ stage for tile scanning, multi-point imaging and slide scanning. 25mm x 25mm x 1mm travel
  • Roper CoolSNAP HQ CCD camera.
  • Solent Scientific temperature controlled chamber, including CO2 delivery, for live cell imaging.

Software

  • SoftWoRx image acquisition and analysis (mirrored on offline deconvolution workstation)