Faculty of Biological Sciences

Multiphoton and Fluorescence-lifetime imaging microscopy (FLIM)

This system is ideally suited for studies on cellular behaviour and structural morphology of biological tissues, either in-vivo in small animal models or in-vitro with excised tissue or fixed tissue sections, cells on membranes or in Petri dishes.

An award has been granted from the Wellcome Trust Institutional Strategic Support Fund in order to widen the use of the Multiphoton/TCSPC system in the LIGHT Building. The normal hourly rate is £31 per hour but in order to widen the use of the system this is being reduced to £10 per hour. This is in order to allow researchers who do not have research grants to cover the costs and enable them to do some pilot studies. This reduced price will be available until 31st January 2017.


  • Optical sectioning of relatively thick tissue sections
  • FLIM
  • Physiological temperature and CO2 control and super-perfusion


  • Upright Zeiss 710


  • 633 nm HeNe
  • 543 nm HeNe
  • 458, 288, 514 nm Argon
  • 690 – 1064 nm Chameleon


  • Air 10x, NA 0.3 (Plan – Apochromat)
  • Air 20x, NA 0.8 (Plan – Apochromat)
  • Oil immersion 63x, NA 1.4 (Plan – Apochromat)
  • Dipping lens 20x, NA 1.0 (Plan – Apochromat)
  • Dipping lens 40x, NA 1.0 (Plan – Apochromat)


Spectral detector with PMTs, BiG detector and external non-descanned detector (NDD) for increased sensitivity

Power-Tau 150 M Ultra-fast time correlated single photon counting system

Software - ZEN2012


  • LIGHT Building 6.09


For further information about using the microscope, training, applications and charges - please contact David Myers at multiphoton@leeds.ac.uk

Some examples of images taken so far...

Optical section of the Collagen arrangement in a decellularised artery

Left: Collagen network in the decellularised artery. Right: The same section (as left) when the incorporated fluorescent-stained Peptides within the Collagen network are visualised


To follow...

Links & protocols

To follow...