Bioimaging Leeds


  • Installed in Aug 2012
  • Confocal imaging of fixed and live cells, FRAP and FRET imaging
  • Imaging UV and visual flurophores (eg DAPI, Hoechst, CFP, GFP, YFP, Alexa 488/568/647)
  • Z-sectioning and 3D imaging
  • Motorised stage for screening plates and slides, tile scanning and point visiting
  • DIC and phase contrast objectives
  • Temperature and CO2 regulation for live cell imaging
  • Control emission detection manually
  • Light path redesigned to achieve greater sensitivity and flexibility
  • 4 diode lasers (405, 488, 555, 639nm) ensure ease of use and low heat production


  • L.C.Miall 5.08


  • See Charges page (internal link only)


  • Axio Observer Z1 inverted stand
  • Motorized stage: Marzhauser scan stage DC 120x100 mm
  • Environmental control (temperature and CO2)


  • 405nm laser, 5mW
  • 488nm laser, 10mW
  • 555nm laser, 10mW
  • 639nm laser, 5mW


  • 405/488/555/639

Spectral detector

  • Two channel collection (photomultiplyers)
  • Variable secondary dichroic (VSD) beamsplitter allows two simultaneous detection channels or 'tunable' emission collection


  • 10x/0.3 Ph1 Plan-Neofluar, M27
  • 20x/0.5 Ph2 Plan Neofluar, M27
  • 40/1.3 Oil DIC Plan Apochromat, M27
  • 63x/1.4 Oil DIC Plan Apochrom, M27at
  • also available 40x/1.2 water immersion Corrected, M27